Restriction map for pF1KSDA1004
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BbeI        BbrPI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspHI       BsrBI       BsrGI       BssHII      
BssSI       BstBI       CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       KasI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII        2257  CGCG                 CG^CG
AccIII       3113  TCCGGA               T^CCGGA
AocI          836  CCTCAGG              CC^T[ACGT]AGG
AspI         2208  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglI         2461  GCCATCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          391  GAGACC               GGTCTC(1/5)
BsaWI        3113  TCCGGA               [AT]^CCGG[AT]
BseAI        3113  TCCGGA               T^CCGGA
BsmI         1921  GAATGC               GAATGC(1/-1)
BspEI        3113  TCCGGA               T^CCGGA
BsrFI        3075  ACCGGC               [GA]^CCGG[CT]
Bst1107I      858  GTATAC               GTA^TAC
BstEII        775  GGTGACC              G^GT[ACGT]ACC
BstPI         775  GGTGACC              G^GT[ACGT]ACC
BstUI        2257  CGCG                 CG^CG
BstXI        1999  CCAAATTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       858  GTATAC               GTA^TAC
Bsu36I        836  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       3075  ACCGGC               [GA]^CCGG[CT]
Eco81I        836  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1047  CCTAACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       775  GGTGACC              G^GT[ACGT]ACC
EcoRI         237  GAATTC               G^AATTC
HindIII      1561  AAGCTT               A^AGCTT
HpaI         1644  GTTAAC               GTT^AAC
MroI         3113  TCCGGA               T^CCGGA
MvnI         2257  CGCG                 CG^CG
PaeR7I        436  CTCGAG               C^TCGAG
PflFI        2208  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         714  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        3208  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI          593  AGTACT               AGT^ACT
SexAI        3087  ACCAGGT              A^CC[AT]GGT
SfiI         2460  GGCCATCACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2195  CCCGGG               CCC^GGG
Tth111I      2208  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        714  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          436  CTCGAG               C^TCGAG
XmaCI        2195  CCCGGG               C^CCGGG
XmaI         2195  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I         46  GGTACC               G^GTACC
              649  GGTACC              
AccI          505  GTAGAC               GT^[AC][GT]AC
              858  GTATAC              
AflIII        512  ACATGT               A^C[GA][CT]GT
             1855  ACATGT              
Aor51HI      1013  AGCGCT               AGC^GCT
             2264  AGCGCT              
Asp700I        16  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1375  GAAGGCCTTC          
Asp718I        46  GGTACC               G^GTACC
              649  GGTACC              
AvaI          436  CTCGAG               C^[CT]CG[GA]G
             2195  CCCGGG              
BalI          517  TGGCCA               TGG^CCA
             1546  TGGCCA              
BamHI         267  GGATCC               G^GATCC
             1317  GGATCC              
BcgI         3400  CGAAAAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3390  GCAGATCACTCG        
BsmBI        1698  GAGACG               CGTCTC(1/5)
             2663  GAGACG              
BsoBI         436  CTCGAG               C^[CT]CG[GA]G
             2195  CCCGGG              
BspLU11I      512  ACATGT               A^CATGT
             1855  ACATGT              
BspMI        1228  ACCTGC               ACCTGC(4/8)
             3304  GCAGGT              
Eco47III     1013  AGCGCT               AGC^GCT
             2264  AGCGCT              
HaeII        1013  AGCGCT               [GA]GCGC^[CT]
             2264  AGCGCT              
HincII       1198  GTCAAC               GT[CT]^[GA]AC
             1644  GTTAAC              
HindII       1198  GTCAAC               GT[CT]^[GA]AC
             1644  GTTAAC              
KpnI           46  GGTACC               GGTAC^C
              649  GGTACC              
MluNI         517  TGGCCA               TGG^CCA
             1546  TGGCCA              
MscI          517  TGGCCA               TGG^CCA
             1546  TGGCCA              
NcoI          365  CCATGG               C^CATGG
             2312  CCATGG              
PpuMI        3010  AGGACCC              [GA]G^G[AT]CC[CT]
             3044  AGGACCC             
PvuII        1661  CAGCTG               CAG^CTG
             2994  CAGCTG              
StuI         1377  AGGCCT               AGG^CCT
             3141  AGGCCT              
XmnI           16  GAAAGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1375  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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