Restriction map for pF1KSDA0992
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        AlwI        AlwNI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BciVI       BfrI        BglI        
BlnI        BsaBI       BsaWI       BseAI       BsgI        
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrGI       BssSI       BstAPI      BstBI       BstXI       
ClaI        CpoI        Eam1105I    EcoNI       EcoRI       
EcoRV       EcoT22I     FseI        FspI        HindIII     
KasI        MamI        MfeI        MluI        MroI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        Tth111I     
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       1610  GGTACC               G^GTACC
AcsI          554  AAATTC               [GA]^AATT[CT]
Alw44I       1435  GTGCAC               G^TGCAC
Aor51HI       430  AGCGCT               AGC^GCT
ApaI           97  GGGCCC               GGGCC^C
ApaLI        1435  GTGCAC               G^TGCAC
ApoI          554  AAATTC               [GA]^AATT[CT]
Asp718I      1610  GGTACC               G^GTACC
BalI          630  TGGCCA               TGG^CCA
BbrPI        1466  CACGTG               CAC^GTG
BbsI          283  GTCTTC               GAAGAC(2/6)
BcgI         1912  GCAGCACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          223  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      223  GCTGAGC              GC^T[ACGT]AGC
BpuAI         283  GTCTTC               GAAGAC(2/6)
BsaAI        1466  CACGTG               [CT]AC^GT[GA]
BsiWI        1389  CGTACG               C^GTACG
BsmBI        1232  GAGACG               CGTCTC(1/5)
BsmI          911  GAATGC               GAATGC(1/-1)
BspMI        1036  ACCTGC               ACCTGC(4/8)
Bst1107I     1782  GTATAC               GTA^TAC
BstEII       1346  GGTTACC              G^GT[ACGT]ACC
BstPI        1346  GGTTACC              G^GT[ACGT]ACC
BstZ17I      1782  GTATAC               GTA^TAC
CelII         223  GCTGAGC              GC^T[ACGT]AGC
DraI          970  TTTAAA               TTT^AAA
DraIII       1845  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1330  GACTGCAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          718  GAAGAG               CTCTTC(1/4)
Eco47III      430  AGCGCT               AGC^GCT
EcoO65I      1346  GGTTACC              G^GT[ACGT]ACC
HpaI          642  GTTAAC               GTT^AAC
KpnI         1610  GGTACC               GGTAC^C
Ksp632I       718  GAAGAG               CTCTTC(1/4)
KspI          416  CCGCGG               CCGC^GG
MluNI         630  TGGCCA               TGG^CCA
MscI          630  TGGCCA               TGG^CCA
MslI         1189  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI          767  GACTC                GAGTC(4/5)
PmaCI        1466  CACGTG               CAC^GTG
PmlI         1466  CACGTG               CAC^GTG
PshAI         362  GACAGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1320  CTGCAG               CTGCA^G
PvuII         753  CAGCTG               CAG^CTG
SacII         416  CCGCGG               CCGC^GG
SalI          668  GTCGAC               G^TCGAC
SfcI         1320  CTGCAG               C^T[GA][CT]AG
SgrAI         455  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          357  CCCGGG               CCC^GGG
SphI         1712  GCATGC               GCATG^C
SspI          703  AATATT               AAT^ATT
StuI         1204  AGGCCT               AGG^CCT
XbaI         1213  TCTAGA               T^CTAGA
XmaCI         357  CCCGGG               C^CCGGG
XmaI          357  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          668  GTCGAC               GT^[AC][GT]AC
             1782  GTATAC              
Asp700I      1243  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1515  GAACTCATTC          
BbvCI         426  CCTCAGC              CCTCAGC(-5/-2)
             1895  CCTCAGC             
BclI         1002  TGATCA               T^GATCA
             1445  TGATCA              
BsaI          800  GGTCTC               GGTCTC(1/5)
             1175  GGTCTC              
BseRI         246  CTCCTC               GAGGAG(10/8)
              249  CTCCTC              
BsiEI         183  CGGCCG               CG[GA][CT]^CG
              461  CGGCCG              
BspLU11I      928  ACATGT               A^CATGT
             1486  ACATGT              
BsrBI          18  CCGCTC               CCGCTC(-3/-3)
              346  CCGCTC              
BssHII          5  GCGCGC               G^CGCGC
               61  GCGCGC              
EagI          183  CGGCCG               C^GGCCG
              461  CGGCCG              
EclXI         183  CGGCCG               C^GGCCG
              461  CGGCCG              
Eco52I        183  CGGCCG               C^GGCCG
              461  CGGCCG              
EcoT14I       485  CCAAGG               C^C[AT][AT]GG
             1994  CCTTGG              
FbaI         1002  TGATCA               T^GATCA
             1445  TGATCA              
HaeII         220  GGCGCT               [GA]GCGC^[CT]
              430  AGCGCT              
MspA1I        416  CCGCGG               C[AC]G^C[GT]G
              753  CAGCTG              
StyI          485  CCAAGG               C^C[AT][AT]GG
             1994  CCTTGG              
XcmI          955  CCAAAGATCTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1357  CCAGATCTAAGCTGG     
XmnI         1243  GAACCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1515  GAACTCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AocI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglII       BmyI        BpmI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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