Restriction map for pF1KSDA0964
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvrII       BamHI       BbrPI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BsgI        
BsiWI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
CelII       CpoI        DraI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
RcaI        RsrII       SalI        SapI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SspBI       
SspI        SwaI        Tru9I       XbaI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI         2557  AAATTC               [GA]^AATT[CT]
AgeI         1686  ACCGGT               A^CCGGT
AlwI         1377  GATCC                GGATC(4/5)
AocI         1888  CCTGAGG              CC^T[ACGT]AGG
ApaI         1068  GGGCCC               GGGCC^C
ApoI         2557  AAATTC               [GA]^AATT[CT]
AspI         1576  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1091  TGCGCA               TGC^GCA
BalI         1392  TGGCCA               TGG^CCA
BciVI        2139  GGATAC               GTATCC(6/5)
BsrDI         497  GCAATG               GCAATG(2/0)
Bsu36I       1888  CCTGAGG              CC^T[ACGT]AGG
DraIII       1865  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1797  GACTAGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1061  CGGCCG               C^GGCCG
EclXI        1061  CGGCCG               C^GGCCG
Eco52I       1061  CGGCCG               C^GGCCG
Eco81I       1888  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2037  CCTCTCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2671  GATATC               GAT^ATC
FspI         1091  TGCGCA               TGC^GCA
MaeII         863  ACGT                 A^CGT
MluNI        1392  TGGCCA               TGG^CCA
MscI         1392  TGGCCA               TGG^CCA
MslI          343  CACCGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1115  CCATGG               C^CATGG
PaeR7I       2341  CTCGAG               C^TCGAG
PflFI        1576  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1686  ACCGGT               A^CCGGT
PvuI         2063  CGATCG               CGAT^CG
SacI          615  GAGCTC               GAGCT^C
SbfI          993  CCTGCAGG             CCTGCA^GG
ScaI         1741  AGTACT               AGT^ACT
SexAI         422  ACCTGGT              A^CC[AT]GGT
SmaI          139  CCCGGG               CCC^GGG
SphI         1088  GCATGC               GCATG^C
Sse8387I      993  CCTGCAGG             CCTGCA^GG
TfiI         1258  GATTC                G^A[AT]TC
Tth111I      1576  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2341  CTCGAG               C^TCGAG
XmaCI         139  CCCGGG               C^CCGGG
XmaI          139  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI         615  GAGCTC               G[AT]GC[AT]^C
             1023  GAGCAC              
BbeI          646  GGCGCC               GGCGC^C
             1181  GGCGCC              
BbsI          921  GAAGAC               GAAGAC(2/6)
             1674  GAAGAC              
BpuAI         921  GAAGAC               GAAGAC(2/6)
             1674  GAAGAC              
BsaWI        1686  ACCGGT               [AT]^CCGG[AT]
             2772  TCCGGT              
BsiHKAI       615  GAGCTC               G[AT]GC[AT]^C
             1023  GAGCAC              
BssSI          58  CACGAG               CACGAG(-5/-1)
              907  CACGAG              
KasI          646  GGCGCC               G^GCGCC
             1181  GGCGCC              
KspI         1058  CCGCGG               CCGC^GG
             1172  CCGCGG              
NarI          646  GGCGCC               GG^CGCC
             1181  GGCGCC              
PpuMI        2627  AGGACCT              [GA]G^G[AT]CC[CT]
             2645  GGGACCT             
SacII        1058  CCGCGG               CCGC^GG
             1172  CCGCGG              
SfoI          646  GGCGCC               GGC^GCC
             1181  GGCGCC              
Tsp509I       368  AATT                 ^AATT
             2558  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsiEI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspDI       BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      ClaI        
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        
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