Restriction map for pF1KSDA0964
 (Linear) (Six-base) MAPSORT of: KIAA0964.seq  Check: 3881  from: 1  to: 2967



KIAA0964.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     835     987    2967 
   Size:        835     152    1980

  Fragments arranged by size: 

                1980     835     152
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339    2516    2967 
   Size:        339    2177     451

  Fragments arranged by size: 

                2177     451     339
 
AgeI A'CCGG_T

Cuts at:      0    1686    2967 
   Size:       1686    1281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1048    2967 
   Size:       1048    1919
 
AlwNI CAG_nnn'CTG

Cuts at:      0     673    1215    1888    2544    2631    2893    2967 
   Size:        673     542     673     656      87     262      74

  Fragments arranged by size: 

                 673     673     656     542     262      87      74
 
ApaI G_GGCC'C

Cuts at:      0    1072    2967 
   Size:       1072    1895
 
ApoI r'AATT_y

Cuts at:      0    2557    2967 
   Size:       2557     410
 
AvaI C'yCGr_G

Cuts at:      0     139    1966    2341    2737    2967 
   Size:        139    1827     375     396     230

  Fragments arranged by size: 

                1827     396     375     230     139
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1827    1860    2967 
   Size:       1827      33    1107

  Fragments arranged by size: 

                1827    1107      33
 
BanI G'GyrC_C

Cuts at:      0     243     459     646     999    1181    2076    2967 
   Size:        243     216     187     353     182     895     891

  Fragments arranged by size: 

                 895     891     353     243     216     187     182
 
BanII G_rGCy'C

Cuts at:      0      65     259     406     578     619     877    1072    2967 
   Size:         65     194     147     172      41     258     195    1895

  Fragments arranged by size: 

                1895     258     195     194     172     147      65      41
            
 
BbeI G_GCGC'C

Cuts at:      0     650    1185    2967 
   Size:        650     535    1782

  Fragments arranged by size: 

                1782     650     535
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     928    1681    2967 
   Size:        928     753    1286

  Fragments arranged by size: 

                1286     928     753
 
BbvCI CC'TCA_GC

Cuts at:      0     895     927    1624    2967 
   Size:        895      32     697    1343

  Fragments arranged by size: 

                1343     895     697      32
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1036    1070    1544    1578    2667    2701    2967 
   Size:       1036      34     474      34    1089      34     266

  Fragments arranged by size: 

                1089    1036     474     266      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2133    2967 
   Size:       2133     834
 
BglI GCCn_nnn'nGGC

Cuts at:      0     127     582    1552    1808    1891    2308    2399    2884 
   Size:        127     455     970     256      83     417      91     485

Cuts at:   2884    2967 
   Size:         83

  Fragments arranged by size: 

                 970     485     455     417     256     127      91      83
                  83
 
Bme1580I G_kGCm'C

Cuts at:      0     478     755     850    1004    1072    2538    2967 
   Size:        478     277      95     154      68    1466     429

  Fragments arranged by size: 

                1466     478     429     277     154      95      68
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1377    1604    2967 
   Size:       1377     227    1363

  Fragments arranged by size: 

                1377    1363     227
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     184    2331    2367    2967 
   Size:        184    2147      36     600

  Fragments arranged by size: 

                2147     600     184      36
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1323    2337    2967 
   Size:        490     833    1014     630

  Fragments arranged by size: 

                1014     833     630     490
 
Bpu10I CC'TnA_GC

Cuts at:      0     895     927    1624    1813    2967 
   Size:        895      32     697     189    1154

  Fragments arranged by size: 

                1154     895     697     189      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937    2145    2689    2967 
   Size:        937    1208     544     278

  Fragments arranged by size: 

                1208     937     544     278
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2338    2467    2725    2967 
   Size:       2338     129     258     242

  Fragments arranged by size: 

                2338     258     242     129
 
BsaHI Gr'CG_yC

Cuts at:      0     647    1182    2311    2831    2967 
   Size:        647     535    1129     520     136

  Fragments arranged by size: 

                1129     647     535     520     136
 
BsaWI w'CCGG_w

Cuts at:      0    1686    2772    2967 
   Size:       1686    1086     195

  Fragments arranged by size: 

                1686    1086     195
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     214     244    1504    1534    1567    1597    2197    2227 
   Size:        214      30    1260      30      33      30     600      30

Cuts at:   2227    2967 
   Size:        740

  Fragments arranged by size: 

                1260     740     600     214      33      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265    1585    2073    2196    2340    2967 
   Size:        265    1320     488     123     144     627

  Fragments arranged by size: 

                1320     627     488     265     144     123
 
BseYI C'CCAG_C

Cuts at:      0     199     265     568     666     819     832    1215    1295 
   Size:        199      66     303      98     153      13     383      80

Cuts at:   1295    1507    1855    1881    2361    2537    2967 
   Size:        212     348      26     480     176     430

  Fragments arranged by size: 

                 480     430     383     348     303     212     199     176
                 153      98      80      66      26      13
 
BsiEI CG_ry'CG

Cuts at:      0      84    1064    1852    2066    2967 
   Size:         84     980     788     214     901

  Fragments arranged by size: 

                 980     901     788     214      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     619    1027    2967 
   Size:        619     408    1940

  Fragments arranged by size: 

                1940     619     408
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1042    1480    2390    2967 
   Size:       1042     438     910     577

  Fragments arranged by size: 

                1042     910     577     438
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     259     406     478     578     619     755     850 
   Size:         65     194     147      72     100      41     136      95

Cuts at:    850     877    1004    1027    1072    2538    2967 
   Size:         27     127      23      45    1466     429

  Fragments arranged by size: 

                1466     429     194     147     136     127     100      95
                  72      65      45      41      27      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     835     987    1001    1395    1547    2657    2967 
   Size:        104     731     152      14     394     152    1110     310

  Fragments arranged by size: 

                1110     731     394     310     152     152     104      14
            
 
BsrBI CCG'CTC

Cuts at:      0     558     593    1520    2439    2967 
   Size:        558      35     927     919     528

  Fragments arranged by size: 

                 927     919     558     528      35
 
BsrDI GCAATG_nn'

Cuts at:      0     504    2967 
   Size:        504    2463
 
BsrFI r'CCGG_y

Cuts at:      0     293     643    1082    1615    1686    2230    2370    2802 
   Size:        293     350     439     533      71     544     140     432

Cuts at:   2802    2967 
   Size:        165

  Fragments arranged by size: 

                 544     533     439     432     350     293     165     140
                  71
 
BssSI C'ACGA_G

Cuts at:      0      58     907    2967 
   Size:         58     849    2060

  Fragments arranged by size: 

                2060     849      58
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312     349    1360    1390    2967 
   Size:        312      37    1011      30    1577

  Fragments arranged by size: 

                1577    1011     312      37      30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1889    2967 
   Size:       1889    1078
 
BtgI C'CryG_G

Cuts at:      0     107     375     393    1058    1115    1172    2967 
   Size:        107     268      18     665      57      57    1795

  Fragments arranged by size: 

                1795     665     268     107      57      57      18
 
BtsI GCAGTG_nn'

Cuts at:      0     666    1467    1501    2538    2813    2967 
   Size:        666     801      34    1037     275     154

  Fragments arranged by size: 

                1037     801     666     275     154      34
 
ClaI AT'CG_AT

Cuts at:      0    1996    2066    2225    2967 
   Size:       1996      70     159     742

  Fragments arranged by size: 

                1996     742     159      70
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     375     410     474     495     509     530    2967 
   Size:        375      35      64      21      14      21    2437

  Fragments arranged by size: 

                2437     375      64      35      21      21      14
 
DraIII CAC_nnn'GTG

Cuts at:      0    1870    2967 
   Size:       1870    1097
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1803    2967 
   Size:       1803    1164
 
EaeI y'GGCC_r

Cuts at:      0     408    1061    1392    2876    2967 
   Size:        408     653     331    1484      91

  Fragments arranged by size: 

                1484     653     408     331      91
 
EagI C'GGCC_G

Cuts at:      0    1061    2967 
   Size:       1061    1906
 
EarI CTCTTCn'nnn_

Cuts at:      0    1295    2737    2747    2967 
   Size:       1295    1442      10     220

  Fragments arranged by size: 

                1442    1295     220      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     406    2967 
   Size:        406    2561
 
EcoICRI GAG'CTC

Cuts at:      0     617    2967 
   Size:        617    2350
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339     490    1323    2337    2516    2967 
   Size:        339     151     833    1014     179     451

  Fragments arranged by size: 

                1014     833     451     339     179     151
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2041    2967 
   Size:       2041     926
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1068    1069    1893    2244    2384    2574    2628    2646 
   Size:       1068       1     824     351     140     190      54      18

Cuts at:   2646    2967 
   Size:        321

  Fragments arranged by size: 

                1068     824     351     321     190     140      54      18
                   1
 
EcoRV GAT'ATC

Cuts at:      0    2673    2967 
   Size:       2673     294
 
FspI TGC'GCA

Cuts at:      0    1093    2967 
   Size:       1093    1874
 
HaeII r_GCGC'y

Cuts at:      0     650    1185    2850    2967 
   Size:        650     535    1665     117

  Fragments arranged by size: 

                1665     650     535     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1567    1599    2119    2151    2809    2841    2967 
   Size:       1567      32     520      32     658      32     126

  Fragments arranged by size: 

                1567     658     520     126      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     646    1181    2967 
   Size:        646     535    1786

  Fragments arranged by size: 

                1786     646     535
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     620    1805    2967 
   Size:         62     558    1185    1162

  Fragments arranged by size: 

                1185    1162     558      62
 
MscI TGG'CCA

Cuts at:      0    1394    2967 
   Size:       1394    1573
 
MslI CAynn'nnrTG

Cuts at:      0     347    2967 
   Size:        347    2620
 
MspA1I CmG'CkG

Cuts at:      0     189     333     441     481    1060    1097    1174    1343 
   Size:        189     144     108      40     579      37      77     169

Cuts at:   1343    1480    2658    2717    2724    2967 
   Size:        137    1178      59       7     243

  Fragments arranged by size: 

                1178     579     243     189     169     144     137     108
                  77      59      40      37       7
 
NaeI GCC'GGC

Cuts at:      0     645    1084    2372    2804    2967 
   Size:        645     439    1288     432     163

  Fragments arranged by size: 

                1288     645     439     432     163
 
NarI GG'CG_CC

Cuts at:      0     647    1182    2967 
   Size:        647     535    1785

  Fragments arranged by size: 

                1785     647     535
 
NcoI C'CATG_G

Cuts at:      0    1115    2967 
   Size:       1115    1852
 
NgoMIV G'CCGG_C

Cuts at:      0     643    1082    2370    2802    2967 
   Size:        643     439    1288     432     165

  Fragments arranged by size: 

                1288     643     439     432     165
 
NspI r_CATG'y

Cuts at:      0     759    1092    1272    1918    2967 
   Size:        759     333     180     646    1049

  Fragments arranged by size: 

                1049     759     646     333     180
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     163     673    2631    2717    2967 
   Size:        163     510    1958      86     250

  Fragments arranged by size: 

                1958     510     250     163      86
 
PfoI T'CCnGG_A

Cuts at:      0     311    2189    2945    2967 
   Size:        311    1878     756      22

  Fragments arranged by size: 

                1878     756     311      22
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2476    2508    2967 
   Size:       2476      32     459

  Fragments arranged by size: 

                2476     459      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2628    2646    2967 
   Size:       2628      18     321

  Fragments arranged by size: 

                2628     321      18
 
PspOMI G'GGCC_C

Cuts at:      0    1068    2967 
   Size:       1068    1899
 
PstI C_TGCA'G

Cuts at:      0     998    1462    1592    2426    2967 
   Size:        998     464     130     834     541

  Fragments arranged by size: 

                 998     834     541     464     130
 
PvuI CG_AT'CG

Cuts at:      0    2066    2967 
   Size:       2066     901
 
PvuII CAG'CTG

Cuts at:      0     189     333    1343    2658    2717    2724    2967 
   Size:        189     144    1010    1315      59       7     243

  Fragments arranged by size: 

                1315    1010     243     189     144      59       7
 
SacI G_AGCT'C

Cuts at:      0     619    2967 
   Size:        619    2348
 
SacII CC_GC'GG

Cuts at:      0    1061    1175    2967 
   Size:       1061     114    1792

  Fragments arranged by size: 

                1792    1061     114
 
SbfI CC_TGCA'GG

Cuts at:      0     998    2967 
   Size:        998    1969
 
ScaI AGT'ACT

Cuts at:      0    1743    2967 
   Size:       1743    1224
 
SexAI A'CCwGG_T

Cuts at:      0     422    2967 
   Size:        422    2545
 
SfcI C'TryA_G

Cuts at:      0     994    1458    1588    2422    2653    2967 
   Size:        994     464     130     834     231     314

  Fragments arranged by size: 

                 994     834     464     314     231     130
 
SfoI GGC'GCC

Cuts at:      0     648    1183    2967 
   Size:        648     535    1784

  Fragments arranged by size: 

                1784     648     535
 
SmaI CCC'GGG

Cuts at:      0     141    2967 
   Size:        141    2826
 
SmlI C'TyrA_G

Cuts at:      0     952    2160    2341    2704    2967 
   Size:        952    1208     181     363     263

  Fragments arranged by size: 

                1208     952     363     263     181
 
SphI G_CATG'C

Cuts at:      0    1092    2967 
   Size:       1092    1875
 
StuI AGG'CCT

Cuts at:      0       8     115    1369    2824    2967 
   Size:          8     107    1254    1455     143

  Fragments arranged by size: 

                1455    1254     143     107       8
 
StyI C'CwwG_G

Cuts at:      0     386     455     563     837     935    1115    2967 
   Size:        386      69     108     274      98     180    1852

  Fragments arranged by size: 

                1852     386     274     180     108      98      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      94    1179    2967 
   Size:         94    1085    1788

  Fragments arranged by size: 

                1788    1085      94
 
TatI w'GTAC_w

Cuts at:      0    1741    2967 
   Size:       1741    1226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     214     246    2098    2130    2197    2229    2437    2469 
   Size:        214      32    1852      32      67      32     208      32

Cuts at:   2469    2967 
   Size:        498

  Fragments arranged by size: 

                1852     498     214     208      67      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1579    2967 
   Size:       1579    1388
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     373     840    2333    2967 
   Size:        373     467    1493     634

  Fragments arranged by size: 

                1493     634     467     373
 
XhoI C'TCGA_G

Cuts at:      0    2341    2967 
   Size:       2341     626
 
XmaI C'CCGG_G

Cuts at:      0     139    2967 
   Size:        139    2828
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI   Bsu36I
     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII     SacI    SacII     SbfI     ScaI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaBI
     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I    BtgZI     DraI    EcoRI     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI    RsrII     SalI
    SanDI     SapI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XmnI     ZraI
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