GENSCAN 1.0	Date run:  3-Nov-116	Time: 04:19:32

Sequence gi568815578f:49715008_49987933 : 272926 bp : 46.92% C+G : Isochore 2 (43 - 51 C+G%)

Parameter matrix: HumanIso.smat

Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

 1.01 Init +   6629   6673   45  1  0   70   94    28 0.200   2.28
 1.02 Intr +  24298  24423  126  0  0   89   60    24 0.137   0.58
 1.03 Intr +  42870  42986  117  2  0  127   89   -40 0.199   0.66
 1.04 Intr +  46159  46205   47  0  2  103   96     6 0.055   0.11
 1.05 Intr +  81700  81732   33  1  0  120   80    17 0.000   1.44
 1.06 Intr +  97843  97941   99  1  0   87   64    97 0.861   6.43
 1.07 Intr + 100001 100182  182  2  2  101   36   163 0.936  11.91
 1.08 Intr + 108054 108134   81  1  0   98   97    15 0.800   3.01
 1.09 Intr + 114769 114984  216  2  0   53   39   150 0.594   5.08
 1.10 Intr + 115094 115324  231  0  0   26    7   205 0.582   3.94
 1.11 Intr + 115703 115779   77  0  2   70   38    69 0.507  -0.67
 1.12 Term + 115932 116063  132  2  0   16   52   179 0.452   5.19
 1.13 PlyA + 118432 118437    6                               1.05

 2.02 PlyA - 118906 118901    6                               1.05
 2.01 Sngl - 130551 130156  396  0  0   78   54   253 0.959  17.15
 2.00 Prom - 133237 133198   40                              -6.36

 3.00 Prom + 137630 137669   40                              -3.16
 3.01 Init + 139911 140134  224  2  2   78  -13   153 0.776   0.26
 3.02 Intr + 140431 140574  144  1  0   95  108   189 0.958  21.00
 3.03 Intr + 144493 144596  104  1  2  110   61   -12 0.216  -1.88
 3.04 Intr + 149721 149837  117  1  0   84  111    35 0.794   5.84
 3.05 Intr + 159698 159814  117  0  0  112   89   149 0.486  17.84
 3.06 Intr + 168839 169059  221  0  2   89   49   607 0.915  54.92
 3.07 Intr + 171745 171891  147  0  0   69   59   322 0.846  27.83
 3.08 Term + 172732 172929  198  0  0  113   48   245 0.760  20.40
 3.09 PlyA + 176410 176415    6                               1.05

 4.03 PlyA - 180699 180694    6                               1.05
 4.02 Term - 191838 190612 1227  0  0  126   41  1451 0.999 135.82
 4.01 Init - 193483 193148  336  1  0   33  100   473 0.379  40.28
 4.00 Prom - 194965 194926   40                              -7.76

 5.00 Prom + 195266 195305   40                              -3.16
 5.01 Init + 196704 196845  142  2  1   68   77    52 0.510   2.40
 5.02 Term + 200479 200705  227  2  2   59   43   208 0.718  10.34
 5.03 PlyA + 201178 201183    6                               1.05

 6.00 Prom + 203633 203672   40                              -6.76
 6.01 Init + 205694 205767   74  1  2   63   68    70 0.282   1.05
 6.02 Intr + 213718 213808   91  1  1  117  117     9 0.771   6.70
 6.03 Intr + 221271 221528  258  2  0   69   49   214 0.014  13.26
 6.04 Intr + 226576 226704  129  0  0   84   48   128 0.501   9.29
 6.05 Intr + 230390 230481   92  1  2   63   94    85 0.528   5.39
 6.06 Intr + 231129 231243  115  0  1   92   89    56 0.998   6.55
 6.07 Intr + 234241 234348  108  0  0   46  103   125 0.994  10.28
 6.08 Intr + 237069 237104   36  2  0  100   41    87 0.011   3.66
 6.09 Intr + 241796 242230  435  1  0   74  -56   582 0.002  36.18
 6.10 Term + 242558 243025  468  1  0  -32   46   422 0.014  20.97
 6.11 PlyA + 243065 243070    6                               1.05

 7.00 Prom + 248644 248683   40                              -3.76
 7.01 Init + 255746 255880  135  1  0   84   93    53 0.477   5.68
 7.02 Intr + 266511 266593   83  2  2   94   77    62 0.878   4.14
 7.03 Intr + 267721 267879  159  1  0   57   46   151 0.924   6.90
 7.04 Intr + 268076 268134   59  2  2  102   96   127 0.740  13.43
 7.05 Intr + 268817 269344  528  0  0  137   92   512 0.827  49.41

Suboptimal exons with probability > 0.800

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

S.001 Term + 237069 237134   66  2  0  100   53   167 0.955  12.34
S.002 Sngl + 242615 243025  411  1  0   74   46   362 0.976  26.89


Predicted peptide sequence(s):

Predicted coding sequence(s):


>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_1|461_aa
MSPGHMVYKRGFGEQALRPSGGILRNLGGGSHVPTALLGTVHPKPGPTGAASGMAEETGS
CSVTRLEYSGAIMAHCSLNLLGSSNPPSSVPKQLGQVQAILLTPPPDSWEHRCFSLQNGN
NASGSRKQKRVLLAPRLRTRWSWKLRRMGEKMAEEERFPNTTHEGFNVTLHTTLVVTTKL
VLPTPGKPILPVQTGEQAQQEEQSSGMTIFFSLLVLAICIILVHLLIRYRLHFLPESVAV
VSLVRREAGQTQVMRSANSLSEGKVKKIMGDGKKGVSSGSSETGSKSPLKRIQEQSPQKR
GQPPKNEKNVTIVESAKQAISVCYQAITKKLKICEEETGSTSIRAADSTAVNGSITDKKM
GFGGLGLMRSGIVSNLLKMGHTVTVWNRTAKKREVGNAAKMMLIVNIVQGSFIAMITEKD
LCLAIALGNAVNSLTPMAAAANQVYKRAKALDQSNNMSAVS

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_1|1386_bp
atgtctccaggtcacatggtttacaagagaggatttggtgaacaggccctgaggccttct
gggggcatccttagaaacttaggtggaggcagccacgtccccacagctttgctgggcaca
gtacaccccaaaccagggcccactggagcggcatctgggatggccgaggagacaggatct
tgctctgtcaccaggctggagtacagtggtgcaatcatggctcactgcagcctcaatctc
ttgggctcaagcaatcctcctagctcagtccccaagcagctaggacaggttcaagccatc
ctcctgactccacctcctgatagctgggagcacagatgtttctcattgcaaaatgggaat
aatgccagcggaagccggaagcaaaagcgggtcctgctagccccgcggctccgaactcgg
tggtcctggaagctccgcaggatgggggagaagatggcggaagaggagaggttccccaat
acaactcatgagggtttcaatgtcaccctccacaccaccctggttgtcacgacgaaactg
gtgctcccgacccctggcaagcccatcctccccgtgcagacaggggagcaggcccagcaa
gaggagcagtccagcggcatgaccattttcttcagcctccttgtcctagctatctgcatc
atattggtgcatttactgatccgatacagattacatttcttgccagagagtgttgctgtt
gtttctttagtgaggagagaagcaggccaaactcaggtgatgagaagtgcaaacagcctg
tctgaaggaaaagtgaagaagatcatgggagatggaaagaagggggtgtcttcgggctct
tcagagacaggctccaaatcccctctgaaaagaatccaagagcaaagtccccagaagcgg
ggtcagcccccaaagaatgagaagaatgtcaccatcgtggagtccgccaaacaagccatc
tctgtctgttaccaggcaatcacaaagaagttgaaaatatgtgaagaggaaactggttcc
acctccatccgggcagctgacagcacggccgtgaatggcagcatcacagacaaaaagatg
ggatttgggggccttggtctcatgagaagtggaatcgtctctaacttgctaaaaatgggt
cacacagtgactgtctggaaccgcactgccaagaaacgtgaagttggcaacgcagccaag
atgatgctgattgtaaacatagtccaagggagcttcatagccatgatcactgagaaggat
ctctgcttagccattgcgctgggcaatgcggtcaactctctgactcccatggcagctgca
gccaaccaggtgtacaaaagagccaaggcactggaccagtccaacaatatgtccgctgtg
tcctga

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_2|131_aa
MDGPEKVRKAPVGELKETKVLGHMSPSSQDTVYPLYIYQSVSRKTSQCIICEPLNITSAI
FLGKAPIYGKHWLSEPYCLKPLKQPIHHVCSSPEVRHCSPASTEHNQEGAELLLLLLLNY
TQAFKELALQT

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_2|396_bp
atggatgggcctgagaaggttagaaaggccccagtgggggagctgaaagaaacaaaagtc
ctggggcacatgtccccgtcgtcccaggacacagtctatcctctttacatctatcagtct
gtgtccaggaagacgagtcaatgcatcatctgtgagccacttaacatcacttcagccatc
ttcctgggcaaagcacccatttatggcaaacactggctcagtgagccatactgcctgaaa
ccattaaagcagccaattcatcatgtctgctcctctccagaagtcaggcactgcagtcca
gcttccactgagcacaaccaggaaggagcagagctgctgctgctgctgctgctaaattat
acccaagcttttaaggaacttgccttacaaacatga

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_3|423_aa
MASVFLSAVYATHPQSTLGLLLLFDTFLDLANLSAGWGRSRTAFQGAGGEGQSRGQMQEL
MGHLALAALTRGTGRFAFGSLISAVDPVATIAIFNALHVDPVLNMLVFGESILNDAVSIV
LTNTEEKYAPGSCCHLSLVIRIIPGFEEVYFINDHLQIVIYVLKHIDLRKTPSLEFGMMI
IFAYLPYGLAEGISLSGIMAILFSGIVMSHYTHHNLSPVTQILMQQTLRTVAFLCGLRGA
IPYALSLHLDLEPMEKRQLIGTTTIVIVLFTILLLGGSTMPLIRLMDIEDAKAHRRNKKD
VNLSKTEKMGNTVESEHLSELTEEEYEAHYIRRQDLKGFVWLDAKYLNPFFTRRLTQETP
HTKHPVASPPFHGPAPDALTAWLCLSTQDLHHGRIQMKTLTNKWYEEVRQGPSGSEDDEQ
ELL

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_3|1272_bp
atggcctcagtgtttctctcggctgtctacgccactcacccccaaagcacactggggttg
ctgctgctttttgacactttcttagatcttgcaaatctgagtgcaggctggggtcggtca
cggacagcattccaaggggctggtggcgaggggcagagcagaggtcagatgcaggagctc
atgggccatttggctctagctgctctgacccgtggaactgggcgttttgcgtttggctcc
ctaatatctgctgtcgatccagtggccactattgccattttcaatgcacttcatgtggac
cccgtgctcaacatgctggtctttggagaaagtattctcaacgatgcagtctccattgtt
ctgaccaatacagaagaaaaatatgctcctgggagctgttgtcacctttccttggttatc
agaatcatacctggttttgaagaggtatacttcataaacgatcatctccaaattgtcatt
tacgtgctgaagcatattgacttgaggaaaacgccttccttggagtttggcatgatgatc
atttttgcttatctgccttatgggcttgcagaaggaatctcactctcaggcatcatggcc
atccttttctcaggcatcgtgatgtcccactacacgcaccataacctctccccagtcacc
cagatcctcatgcagcagaccctccgcaccgtggccttcttatgtggcctgcggggagcc
atcccctatgccctgagcctacacctggacctggagcccatggagaagcggcagctcatc
ggcaccaccaccatcgtcatcgtgctcttcaccatcctgctgctgggcggcagcaccatg
cccctcattcgcctcatggacatcgaggacgccaaggcacaccgcaggaacaagaaggac
gtcaacctcagcaagactgagaagatgggcaacactgtggagtcggagcacctgtcggag
ctcacggaggaggagtacgaggcccactacatcaggcggcaggaccttaagggcttcgtg
tggctggacgccaagtacctgaaccccttcttcactcggaggctgacgcaggagacaccc
cacacaaaacacccagtagcatcccctcccttccatggccctgcccctgacgccctgacg
gcttggttgtgtctctcgacccaggacctgcaccacgggcgcatccagatgaaaactctc
accaacaagtggtacgaggaggtacgccagggcccctccggctccgaggacgacgagcag
gagctgctctga

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_4|520_aa
MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYR
FRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVY
YVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIH
SQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAK
DYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLT
MASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLL
RNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTH
DSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSC
DACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_4|1563_bp
atggggaagcccagttcaatggatactaaattcaaggatgacttatttcggaagtacgtg
cagttccatgagagcaaagtggataccaccaccagcaggcagcggcctggcagcgatgag
tgcctgcgggtggcagcctcaaccctgctcagcctgcacaaggtggatcccttttatcga
ttccggctgatccagttctatgaggtggtggagagctccttgcgctcgctcagctcctct
agcctgcgggctctgcacggcgccttcagcatgctggagacggtgggcatcaacctcttc
ctctacccgtggaagaaggaattcagaagcatcaagacctacacgggcccttttgtttat
tatgtcaagtcgacattactggaagaggacatccgagccatcctgagctgcatgggctac
acacctgagctgggcactgcatacaagctcagagagctcgtggagaccctccaggtgaag
atggtctcctttgagctctttctggccaaagtcgagtgtgagcagatgctagaaatccac
tcacaagtgaaggacaagggctactccgagctggacattgtgagcgagcgcaagagcagt
gcagaggatgtgcgcggctgctcggacgccctgcggcggcgggcagagggccgggagcac
ctgacggcctccatgtcacgagtggcactccagaagtcggccagcgagcgggcggccaag
gactactacaagccccgcgtgaccaagccctcgaggtcagtggatgcctatgacagctac
tgggagagccggaagccacccctgaaggcctcattgagtcttcggaaggagcctgtggca
acggatgtgggggacgacctcaaggatgagatcatccgcccatccccttcgctgctgacc
atggccagctccccccacggcagcccggatgtgcttccacccgcctcccccagcaacggc
ccggccctgctgcgcggtacctacttctccactcaggatgacgtggatctgtacacagac
tctgaacccagggccacctaccgtcggcaggatgctctgcggccggatgtgtggctgctc
agaaacgatgcccactccctctaccacaagcgctcgccccctgccaaagagtccgccctc
tccaagtgccaaagctgcgggctgtcctgcagctcctccctctgccagcgctgtgacagc
ctgctcacctgtcctccagcttccaagcccagcgccttccccagcaaggcctcgactcat
gacagcctggcccacggggcatctctgcgggagaagtacccaggccagactcagggcctc
gaccgcctcccgcaccttcactccaaatccaagccctccaccacgcccacttcccgctgt
ggcttctgcaaccgcccaggcgccaccaacacctgcacccagtgttcaaaagtctcatgt
gacgcctgcctcagcgcttaccattatgacccctgctacaaaaagagtgagctgcacaag
ttcatgcccaacaaccagctgaactacaagtccacccagctctcccatctcgtgtacaga
tag

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_5|122_aa
MDAAKDVCIHDAITLVSHWYLPRILGDQLESKETGSERWHEVRQVAQGVLRPVPGGQQRP
GNTRPGNDVTMRRARREGGPRLRAGSGKRSMPAADGDYNPEAEDKAEGRRARTKPAEPHF
GA

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_5|369_bp
atggatgctgcaaaggatgtctgcattcacgatgccatcactttagtttctcattggtac
ctcccaagaatcctgggagatcagctggagagcaaagaaacgggctcagaaagatggcat
gaggtacgacaagtagctcaaggggtactgagaccagtacccgggggccagcagcgaccc
ggaaacactcggcccggaaatgatgtcaccatgaggcgggcccgaagagagggtggacca
cggctgcgcgctggctccgggaagcggtcgatgcccgcggccgacggagactacaaccca
gaggcggaggacaaagcggaaggccgaagagcgaggacgaaaccggcggaaccgcacttt
ggagcctaa

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_6|601_aa
MVITGVWLRLCLALGARSAPCTKDGQCTVSHDGQRQEILRELERIKEPVFSSQGPLACVR
GAPRGGPPASPAPNRSPPREPRPLGLLLIGRRCAAQSGSKMAAQQRDCGGAAQLAGPAAE
ADPLGRFTCPVCLEVYEKPVQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHG
CRKNIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVE
HCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMN
QAPSYGARPVSSMVSVYAGARGSGSRISESHSTSFWGGMGSGDLAGGMAGDLAGMGGIQN
EKETMQSLNDHLASYLDRMRSLETKNWKPESKIREHLEKKGPQVRDWSHHFKTIEDLRAQ
IFTNTVDNACIVLQINNACLAADDFTIEENTTEVTTQSTEVGTAEMTHRTETCSPVLGDR
PGLHEKSEGQLGEQPEGGGGPLFPADGTAQWDTAVPGVRAGTDPGRGTVPGPGVGSPAEH
KVKLEAEITTYCRLLEDSEDFNLGDALDSRNSMQTIQKTTTRQTVDGKVVSETNDTKVLR
H

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_6|1806_bp
atggttatcacaggagtgtggctgaggctgtgtttggccctgggtgccaggtctgcaccc
tgcactaaggatgggcaatgtacagtaagccatgatgggcagaggcaggaaatacttaga
gaactggaaagaataaaggagccggtgttctcttcgcagggcccgctcgcttgcgtcaga
ggggccccgaggggcggcccacccgctagccccgcccccaaccgctcaccgccccgcgag
ccccgccccctcggcctcctcctcatcggccgccgttgcgcggcgcagagcggcagcaag
atggcggcgcaacagcgggactgcgggggtgctgcgcagctggcggggccggcggcggag
gctgaccccctaggacgcttcacgtgtcccgtgtgcttagaggtgtacgagaagccggta
caggaatgtctgaagccgaagaagcctgtctgtggggtgtgtcgcagcgctctggcacct
ggcgtccgagccgtggagctcgagcggcagatcgagagcacagagacttcttgccatggc
tgccgtaagaatatccggtcccacgtggctacttgttccaaataccagaattacatcatg
gaaggtgtgaaggccaccattaaggatgcatctcttcagccaaggaatgttccaaaccgt
tacacctttccttgtccttactgtcctgagaagaactttgatcaggaaggacttgtggaa
cactgcaaattattccatagcacggataccaaatctgtggtttgtccgatatgtgcctcg
atgccctggggagaccccaactaccgcagcgccaacttcagagagcacatccagcgccgg
caccggttttcttatgacacttttgtggattatgatgttgatgaagaggacatgatgaat
caggcgcccagctatggcgcccggccggtcagcagtatggttagcgtctatgcaggtgcc
cggggctctggttcccggatctccgagtcccactccaccagcttctggggcggcatgggg
tccggggacctggccggggggatggctggggatctggcaggaatgggaggcatccagaac
gagaaggagaccatgcaaagcctgaacgaccatctggcctcctacctggacagaatgagg
agcctggagaccaagaactggaagccggagagcaaaatccgggagcacctggagaagaag
ggaccccaggtcagagactggagccatcacttcaaaaccatcgaggacctgagggctcag
atcttcacaaatactgtggacaatgcctgcattgttctgcagatcaacaatgcctgtctt
gctgctgatgactttacaattgaggagaacactacagaagtcaccacgcagtccaccgag
gttggaactgctgagatgactcacagaactgagacatgcagtccagtccttggagatcga
cctggactccatgagaaatctgaaggccagcttggagaacagcctgagggaggtggaggc
ccgctatttcctgcagatggaacagctcagtgggatactgctgtacctggagtcagagct
ggcacagacccaggcagagggacagtgccaggcccaggagtaggaagccctgctgaacat
aaggtcaagctggaggctgagatcaccacctactgccgcctgctggaagacagcgaggac
ttcaatcttggtgatgccctggacagccgcaactccatgcaaaccatccaaaagaccacc
acccgccagacagtggatggcaaagtggtgtctgagaccaacgacaccaaagttctgaga
cattaa

>gi568815578f:49715008_49987933|GENSCAN_predicted_peptide_7|322_aa
MERAGLYQCSLMGKGPRPETVCEQVCVYEPLSPPTLQVPGDSLGKLEPGEDDFVHGCHTR
HQVTKQTVVLPFSPSTGDDPRCASEPRLGGVPARALTATRREPGQQPAHLLGEWPSAETS
LRLARRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWD
SVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLE
AEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPCVC
GTCGKAFSRPWLLQGHVRTHTX

>gi568815578f:49715008_49987933|GENSCAN_predicted_CDS_7|966_bp
atggagagagcagggctctatcagtgcagtctgatgggtaaggggcctaggcccgagaca
gtctgcgagcaagtgtgcgtgtatgagcccctgagcccgcccaccctgcaagtgcctgga
gactcactggggaagctagaaccaggggaggacgattttgttcacggctgtcacacccgg
caccaagtgactaaacagacagtagttctgcccttcagccccagcaccggggacgacccg
cgctgcgccagcgaaccccgcctcggaggagtccccgcccgggctctcaccgccacgcgg
cgcgagcccggccagcagccggcgcacctgctcggggagtggccttcggcggagacgagc
ctccgattggcgcggaggaagccctccgaccccaatcggaagcctaactacagcgagctg
caggactctaatccagagtttaccttccagcagccctacgaccaggcccacctgctggca
gccatcccacctccggagatcctcaaccccaccgcctcgctgccaatgctcatctgggac
tctgtcctggcgccccaagcccagccaattgcctgggcctcccttcggctccaggagagt
cccagggtggcagagctgacctccctgtcagatgaggacagtgggaaaggctcccagccc
cccagcccaccctcaccggctccttcgtccttctcctctacttcagtctcttccttggag
gccgaggcctatgctgccttcccaggcttgggccaagtgcccaagcagctggcccagctc
tctgaggccaaggatctccaggctcgaaaggccttcaactgcaaatactgcaacaaggaa
tacctcagcctgggtgccctcaagatgcacatccgaagccacacgctgccctgcgtctgc
ggaacctgcgggaaggccttctctaggccctggctgctacaaggccatgtccggacccac
actgnn