Restriction map for pF1KSDA0915
 (Linear) (Six-base) MAPSORT of: KIAA0915.seq  Check: 9670  from: 1  to: 2523



KIAA0915.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2313    2363    2523 
   Size:       2313      50     160

  Fragments arranged by size: 

                2313     160      50
 
AatII G_ACGT'C

Cuts at:      0    1282    2523 
   Size:       1282    1241
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     935    1033    1844    2336    2390    2401    2480 
   Size:        503     432      98     811     492      54      11      79

Cuts at:   2480    2523 
   Size:         43

  Fragments arranged by size: 

                 811     503     492     432      98      79      54      43
                  11
 
AfeI AGC'GCT

Cuts at:      0    1797    2200    2523 
   Size:       1797     403     323

  Fragments arranged by size: 

                1797     403     323
 
AleI CACnn'nnGTG

Cuts at:      0    1327    1582    2523 
   Size:       1327     255     941

  Fragments arranged by size: 

                1327     941     255
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     406     838    1714    2523 
   Size:        406     432     876     809

  Fragments arranged by size: 

                 876     809     432     406
 
AlwNI CAG_nnn'CTG

Cuts at:      0     334     439     604     976    2052    2283    2523 
   Size:        334     105     165     372    1076     231     240

  Fragments arranged by size: 

                1076     372     334     240     231     165     105
 
ApaLI G'TGCA_C

Cuts at:      0     171    2523 
   Size:        171    2352
 
ApoI r'AATT_y

Cuts at:      0    1906    2523 
   Size:       1906     617
 
AvaI C'yCGr_G

Cuts at:      0    2476    2523 
   Size:       2476      47
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1039    1072    2523 
   Size:       1039      33    1451

  Fragments arranged by size: 

                1451    1039      33
 
BamHI G'GATC_C

Cuts at:      0    2097    2523 
   Size:       2097     426
 
BanI G'GyrC_C

Cuts at:      0     675    1898    1934    2450    2500    2523 
   Size:        675    1223      36     516      50      23

  Fragments arranged by size: 

                1223     675     516      50      36      23
 
BanII G_rGCy'C

Cuts at:      0     194     495     668    2523 
   Size:        194     301     173    1855

  Fragments arranged by size: 

                1855     301     194     173
 
BbeI G_GCGC'C

Cuts at:      0    1902    2523 
   Size:       1902     621
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1210    2523 
   Size:       1210    1313
 
BbvCI CC'TCA_GC

Cuts at:      0     234     432    1231    1799    2523 
   Size:        234     198     799     568     724

  Fragments arranged by size: 

                 799     724     568     234     198
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1764    1798    2523 
   Size:       1764      34     725

  Fragments arranged by size: 

                1764     725      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1218    2523 
   Size:       1218    1305
 
BclI T'GATC_A

Cuts at:      0    1368    2523 
   Size:       1368    1155
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1756    2523 
   Size:       1756     767
 
BglII A'GATC_T

Cuts at:      0     897    2523 
   Size:        897    1626
 
BlpI GC'TnA_GC

Cuts at:      0     809    1333    2523 
   Size:        809     524    1190

  Fragments arranged by size: 

                1190     809     524
 
Bme1580I G_kGCm'C

Cuts at:      0     175    2523 
   Size:        175    2348
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       3      87    2523 
   Size:          3      84    2436

  Fragments arranged by size: 

                2436      84       3
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268     575    1139    2523 
   Size:        268     307     564    1384

  Fragments arranged by size: 

                1384     564     307     268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     425     581     682     953    1109    1157    2350 
   Size:        332      93     156     101     271     156      48    1193

Cuts at:   2350    2523 
   Size:        173

  Fragments arranged by size: 

                1193     332     271     173     156     156     101      93
                  48
 
Bpu10I CC'TnA_GC

Cuts at:      0     234     432     494    1231    1799    2523 
   Size:        234     198      62     737     568     724

  Fragments arranged by size: 

                 737     724     568     234     198      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     817    2523 
   Size:        193     624    1706

  Fragments arranged by size: 

                1706     624     193
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     350     526    1549    1890    2523 
   Size:        350     176    1023     341     633

  Fragments arranged by size: 

                1023     633     350     341     176
 
BsaBI GATnn'nnATC

Cuts at:      0     902    2523 
   Size:        902    1621
 
BsaHI Gr'CG_yC

Cuts at:      0    1279    1899    2523 
   Size:       1279     620     624

  Fragments arranged by size: 

                1279     624     620
 
BsaWI w'CCGG_w

Cuts at:      0    2094    2523 
   Size:       2094     429
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133    1285    1315    2523 
   Size:        103      30    1152      30    1208

  Fragments arranged by size: 

                1208    1152     103      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111     709     820     944     956    1009    1102    1567 
   Size:        111     598     111     124      12      53      93     465

Cuts at:   1567    2460    2523 
   Size:        893      63

  Fragments arranged by size: 

                 893     598     465     124     111     111      93      63
                  53      12
 
BseYI C'CCAG_C

Cuts at:      0     195    2084    2205    2408    2523 
   Size:        195    1889     121     203     115

  Fragments arranged by size: 

                1889     203     195     121     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     366    1414    2285    2341    2523 
   Size:        366    1048     871      56     182

  Fragments arranged by size: 

                1048     871     366     182      56
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     597     668    1955    2523 
   Size:        175     422      71    1287     568

  Fragments arranged by size: 

                1287     568     422     175      71
 
BsmI GAATG_Cn'

Cuts at:      0    1752    2523 
   Size:       1752     771
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     194     495     597     668    1955    2523 
   Size:        175      19     301     102      71    1287     568

  Fragments arranged by size: 

                1287     568     301     175     102      71      19
 
BspEI T'CCGG_A

Cuts at:      0    2094    2523 
   Size:       2094     429
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     342     455    1304    2015    2079    2313    2363    2523 
   Size:        342     113     849     711      64     234      50     160

  Fragments arranged by size: 

                 849     711     342     234     160     113      64      50
            
 
BsrBI CCG'CTC

Cuts at:      0     800    1419    1635    2523 
   Size:        800     619     216     888

  Fragments arranged by size: 

                 888     800     619     216
 
BsrFI r'CCGG_y

Cuts at:      0    1607    2523 
   Size:       1607     916
 
BssSI C'ACGA_G

Cuts at:      0     851    2523 
   Size:        851    1672
 
BstEII G'GTnAC_C

Cuts at:      0     374     769    2523 
   Size:        374     395    1754

  Fragments arranged by size: 

                1754     395     374
 
BstYI r'GATC_y

Cuts at:      0     897    2097    2523 
   Size:        897    1200     426

  Fragments arranged by size: 

                1200     897     426
 
BtgI C'CryG_G

Cuts at:      0     398    2523 
   Size:        398    2125
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2050    2523 
   Size:       2050     473
 
BtsI GCAGTG_nn'

Cuts at:      0     803    2523 
   Size:        803    1720
 
DraIII CAC_nnn'GTG

Cuts at:      0    1184    1500    2523 
   Size:       1184     316    1023

  Fragments arranged by size: 

                1184    1023     316
 
EarI CTCTTCn'nnn_

Cuts at:      0     986    1387    1458    1718    1820    2523 
   Size:        986     401      71     260     102     703

  Fragments arranged by size: 

                 986     703     401     260     102      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      49     309    1584    2110    2523 
   Size:         49     260    1275     526     413

  Fragments arranged by size: 

                1275     526     413     260      49
 
EcoICRI GAG'CTC

Cuts at:      0     666    2523 
   Size:        666    1857
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     425     503     581     682     935     953    1033 
   Size:        332      93      78      78     101     253      18      80

Cuts at:   1033    1109    1157    1844    2336    2350    2390    2401    2480 
   Size:         76      48     687     492      14      40      11      79

Cuts at:   2480    2523 
   Size:         43

  Fragments arranged by size: 

                 687     492     332     253     101      93      80      79
                  78      78      76      48      43      40      18      14
                  11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1186    2077    2495    2523 
   Size:       1186     891     418      28

  Fragments arranged by size: 

                1186     891     418      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     103     578     699     708     882    1504    1758    1872 
   Size:        103     475     121       9     174     622     254     114

Cuts at:   1872    2227    2396    2523 
   Size:        355     169     127

  Fragments arranged by size: 

                 622     475     355     254     174     169     127     121
                 114     103       9
 
EcoRI G'AATT_C

Cuts at:      0    1906    2523 
   Size:       1906     617
 
FspI TGC'GCA

Cuts at:      0    1690    2523 
   Size:       1690     833
 
HaeII r_GCGC'y

Cuts at:      0     318    1799    1902    2202    2523 
   Size:        318    1481     103     300     321

  Fragments arranged by size: 

                1481     321     318     300     103
 
HindIII A'AGCT_T

Cuts at:      0    2174    2523 
   Size:       2174     349
 
KasI G'GCGC_C

Cuts at:      0    1898    2523 
   Size:       1898     625
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2466    2523 
   Size:       2466      57
 
MslI CAynn'nnrTG

Cuts at:      0    1327    1582    2523 
   Size:       1327     255     941

  Fragments arranged by size: 

                1327     941     255
 
MspA1I CmG'CkG

Cuts at:      0     199     729    1214    1644    2040    2412    2523 
   Size:        199     530     485     430     396     372     111

  Fragments arranged by size: 

                 530     485     430     396     372     199     111
 
NaeI GCC'GGC

Cuts at:      0    1609    2523 
   Size:       1609     914
 
NarI GG'CG_CC

Cuts at:      0    1899    2523 
   Size:       1899     624
 
NcoI C'CATG_G

Cuts at:      0     398    2523 
   Size:        398    2125
 
NgoMIV G'CCGG_C

Cuts at:      0    1607    2523 
   Size:       1607     916
 
NspI r_CATG'y

Cuts at:      0    1254    1492    1695    2196    2523 
   Size:       1254     238     203     501     327

  Fragments arranged by size: 

                1254     501     327     238     203
 
PasI CC'CwG_GG

Cuts at:      0     100     704    1761    2523 
   Size:        100     604    1057     762

  Fragments arranged by size: 

                1057     762     604     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     185     604    1500    2523 
   Size:        185     419     896    1023

  Fragments arranged by size: 

                1023     896     419     185
 
PfoI T'CCnGG_A

Cuts at:      0    1346    2523 
   Size:       1346    1177
 
PpuMI rG'GwC_Cy

Cuts at:      0     578     699     708     882    2523 
   Size:        578     121       9     174    1641

  Fragments arranged by size: 

                1641     578     174     121       9
 
PstI C_TGCA'G

Cuts at:      0      24    1043    1616    1703    2523 
   Size:         24    1019     573      87     820

  Fragments arranged by size: 

                1019     820     573      87      24
 
PvuII CAG'CTG

Cuts at:      0     199    2412    2523 
   Size:        199    2213     111

  Fragments arranged by size: 

                2213     199     111
 
SacI G_AGCT'C

Cuts at:      0     668    2523 
   Size:        668    1855
 
SanDI GG'GwC_CC

Cuts at:      0     578     708     882    2523 
   Size:        578     130     174    1641

  Fragments arranged by size: 

                1641     578     174     130
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1458    1820    2523 
   Size:       1458     362     703

  Fragments arranged by size: 

                1458     703     362
 
SbfI CC_TGCA'GG

Cuts at:      0    1043    2523 
   Size:       1043    1480
 
SfcI C'TryA_G

Cuts at:      0      20    1039    1560    1612    1699    2523 
   Size:         20    1019     521      52      87     824

  Fragments arranged by size: 

                1019     824     521      87      52      20
 
SfoI GGC'GCC

Cuts at:      0    1900    2523 
   Size:       1900     623
 
SmlI C'TyrA_G

Cuts at:      0     208     832    2476    2523 
   Size:        208     624    1644      47

  Fragments arranged by size: 

                1644     624     208      47
 
SphI G_CATG'C

Cuts at:      0    1254    1492    1695    2523 
   Size:       1254     238     203     828

  Fragments arranged by size: 

                1254     828     238     203
 
StyI C'CwwG_G

Cuts at:      0     398    2523 
   Size:        398    2125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     375     663     757    1093    1161    1333    1753    1858 
   Size:        375     288      94     336      68     172     420     105

Cuts at:   1858    2173    2523 
   Size:        315     350

  Fragments arranged by size: 

                 420     375     350     336     315     288     172     105
                  94      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135     285     317    1136    1168    2523 
   Size:        103      32     150      32     819      32    1355

  Fragments arranged by size: 

                1355     819     150     103      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2324    2523 
   Size:       2324     199
 
XbaI T'CTAG_A

Cuts at:      0     241    2523 
   Size:        241    2282
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1218    2523 
   Size:       1218    1305
 
XhoI C'TCGA_G

Cuts at:      0    2476    2523 
   Size:       2476      47
 
ZraI GAC'GTC

Cuts at:      0    1280    2523 
   Size:       1280    1243
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AleI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BssSI   BstEII    BstYI     BtgI
    BtgZI     BtsI   DraIII     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FspI    HaeII  HindIII     KasI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI     PstI    PvuII     SacI    SanDI     SapI     SbfI
     SfcI     SfoI     SmlI     SphI     StyI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII    BfrBI    BmgBI     BmtI
    BsaAI    BsiEI    BsiWI    BsmBI    BspHI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EaeI     EagI    EcoRV     FalI     FseI    FspAI    Hin4I
   HincII     HpaI     KpnI     MfeI     MluI     MscI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     XmaI     XmnI
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