Restriction map for
pF1KSDA0907
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BamHI BanII BbrPI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI NciI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 38 GGCGCC G[GA]^CG[CT]C
AflIII 343 ACATGT A^C[GA][CT]GT
AocI 1526 CCTCAGG CC^T[ACGT]AGG
AspI 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 38 GGCGCC GGCGC^C
BbsI 811 GTCTTC GAAGAC(2/6)
BbvCI 1108 CCTCAGC CCTCAGC(-5/-2)
BciVI 1432 GGATAC GTATCC(6/5)
BmyI 138 GGGCAC G[AGT]GC[ACT]^C
BpuAI 811 GTCTTC GAAGAC(2/6)
BsaHI 38 GGCGCC G[GA]^CG[CT]C
BsaWI 527 ACCGGA [AT]^CCGG[AT]
BsmBI 1383 GAGACG CGTCTC(1/5)
BsmI 240 GAATGC GAATGC(1/-1)
Bsp1286I 138 GGGCAC G[AGT]GC[ACT]^C
BspHI 428 TCATGA T^CATGA
BspLU11I 343 ACATGT A^CATGT
BsrFI 1185 GCCGGC [GA]^CCGG[CT]
BstAPI 1554 GCAGTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1526 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1185 GCCGGC [GA]^CCGG[CT]
DraII 1624 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 855 TGGCCG [CT]^GGCC[GA]
Eco81I 1526 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1624 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 682 ATGCAT ATGCA^T
HaeII 38 GGCGCC [GA]GCGC^[CT]
Hin1I 38 GGCGCC G[GA]^CG[CT]C
KasI 38 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
NaeI 1185 GCCGGC GCC^GGC
NarI 38 GGCGCC GG^CGCC
NcoI 1641 CCATGG C^CATGG
NdeI 1453 CATATG CA^TATG
NgoMIV 1185 GCCGGC G^CCGGC
NheI 1252 GCTAGC G^CTAGC
NsiI 682 ATGCAT ATGCA^T
PflFI 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1624 GGGACCT [GA]G^G[AT]CC[CT]
PstI 404 CTGCAG CTGCA^G
RcaI 428 TCATGA T^CATGA
SacII 5 CCGCGG CCGC^GG
SexAI 302 ACCTGGT A^CC[AT]GGT
SfcI 404 CTGCAG C^T[GA][CT]AG
SfoI 38 GGCGCC GGC^GCC
Tth111I 1262 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 6 CGCG CG^CG
15 CGCG
AvaI 362 CTCGAG C^[CT]CG[GA]G
416 CTCGAG
BsgI 1240 GTGCAG GTGCAG(16/14)
1502 GTGCAG
BsmAI 1383 GAGAC GTCTC(1/5)
642 GTCTC
BsoBI 362 CTCGAG C^[CT]CG[GA]G
416 CTCGAG
BstEII 111 GGTCACC G^GT[ACGT]ACC
1087 GGTTACC
BstPI 111 GGTCACC G^GT[ACGT]ACC
1087 GGTTACC
BstUI 6 CGCG CG^CG
15 CGCG
BstXI 203 CCATGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
548 CCAATGGAGTGG
CfoI 14 GCGC GCG^C
39 GCGC
DsaI 5 CCGCGG C^C[GA][CT]GG
1641 CCATGG
EarI 79 CTCTTC CTCTTC(1/4)
1380 GAAGAG
EcoNI 265 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1003 CCTTTACCAGG
EcoO65I 111 GGTCACC G^GT[ACGT]ACC
1087 GGTTACC
EcoT14I 164 CCTTGG C^C[AT][AT]GG
1641 CCATGG
HapII 528 CCGG C^CGG
1186 CCGG
HhaI 14 GCGC GCG^C
39 GCGC
HinP1I 14 GCGC G^CGC
39 GCGC
HpaII 528 CCGG C^CGG
1186 CCGG
Ksp632I 79 CTCTTC CTCTTC(1/4)
1380 GAAGAG
MslI 204 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1449 CATTCATATG
MspI 528 CCGG C^CGG
1186 CCGG
MvnI 6 CGCG CG^CG
15 CGCG
NspI 343 ACATGT [GA]CATG^[CT]
720 ACATGC
PaeR7I 362 CTCGAG C^TCGAG
416 CTCGAG
StuI 273 AGGCCT AGG^CCT
909 AGGCCT
StyI 164 CCTTGG C^C[AT][AT]GG
1641 CCATGG
XbaI 714 TCTAGA T^CTAGA
970 TCTAGA
XhoI 362 CTCGAG C^TCGAG
416 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AseI AsnI AvaII BanI BbvI
BfaI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I