Restriction map for pF1KSDA0893
 (Linear) (Six-base) MAPSORT of: KIAA0893.seq  Check: 106  from: 1  to: 2757



KIAA0893.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     178     280     727    1679    2757 
   Size:         29     149     102     447     952    1078

  Fragments arranged by size: 

                1078     952     447     149     102      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     327    2757 
   Size:        327    2430
 
ApaLI G'TGCA_C

Cuts at:      0    1925    2757 
   Size:       1925     832
 
ApoI r'AATT_y

Cuts at:      0      66     226     331     520     625     989    1875    2107 
   Size:         66     160     105     189     105     364     886     232

Cuts at:   2107    2757 
   Size:        650

  Fragments arranged by size: 

                 886     650     364     232     189     160     105     105
                  66
 
AseI AT'TA_AT

Cuts at:      0    1799    2757 
   Size:       1799     958
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1124    1157    2757 
   Size:       1124      33    1600

  Fragments arranged by size: 

                1600    1124      33
 
BamHI G'GATC_C

Cuts at:      0    1984    2757 
   Size:       1984     773
 
BanII G_rGCy'C

Cuts at:      0     320    2562    2757 
   Size:        320    2242     195

  Fragments arranged by size: 

                2242     320     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     756     906    1817    2757 
   Size:        756     150     911     940

  Fragments arranged by size: 

                 940     911     756     150
 
BclI T'GATC_A

Cuts at:      0    1395    2757 
   Size:       1395    1362
 
BfrBI ATG'CAT

Cuts at:      0    2118    2757 
   Size:       2118     639
 
BglII A'GATC_T

Cuts at:      0    2100    2757 
   Size:       2100     657
 
Bme1580I G_kGCm'C

Cuts at:      0    1929    2757 
   Size:       1929     828
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2273    2423    2757 
   Size:       2273     150     334

  Fragments arranged by size: 

                2273     334     150
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     920    2757 
   Size:        920    1837
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     668    1684    1718    1871    2025    2210    2310 
   Size:        121     547    1016      34     153     154     185     100

Cuts at:   2310    2576    2757 
   Size:        266     181

  Fragments arranged by size: 

                1016     547     266     185     181     154     153     121
                 100      34
 
Bpu10I CC'TnA_GC

Cuts at:      0    1124    1940    2757 
   Size:       1124     816     817

  Fragments arranged by size: 

                1124     817     816
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1105    2757 
   Size:       1105    1652
 
BsaBI GATnn'nnATC

Cuts at:      0     719    2757 
   Size:        719    2038
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1608    1638    1998    2028    2757 
   Size:       1608      30     360      30     729

  Fragments arranged by size: 

                1608     729     360      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     845    1616    2183    2504    2757 
   Size:        845     771     567     321     253

  Fragments arranged by size: 

                 845     771     567     321     253
 
BseYI C'CCAG_C

Cuts at:      0     320     469    2757 
   Size:        320     149    2288

  Fragments arranged by size: 

                2288     320     149
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2423    2700    2757 
   Size:       2423     277      57

  Fragments arranged by size: 

                2423     277      57
 
BsiHKAI G_wGCw'C

Cuts at:      0    1218    1929    2562    2660    2757 
   Size:       1218     711     633      98      97

  Fragments arranged by size: 

                1218     711     633      98      97
 
BsmI GAATG_Cn'

Cuts at:      0     619    2757 
   Size:        619    2138
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2443    2757 
   Size:       2443     314
 
Bsp1286I G_dGCh'C

Cuts at:      0     320    1218    1929    2562    2660    2757 
   Size:        320     898     711     633      98      97

  Fragments arranged by size: 

                 898     711     633     320      98      97
 
BspHI T'CATG_A

Cuts at:      0     978    2757 
   Size:        978    1779
 
BsrBI CCG'CTC

Cuts at:      0     932    2757 
   Size:        932    1825
 
BsrDI GCAATG_nn'

Cuts at:      0     361    1513    1533    2757 
   Size:        361    1152      20    1224

  Fragments arranged by size: 

                1224    1152     361      20
 
BsrFI r'CCGG_y

Cuts at:      0    1947    2757 
   Size:       1947     810
 
BssSI C'ACGA_G

Cuts at:      0     479    2757 
   Size:        479    2278
 
BstYI r'GATC_y

Cuts at:      0     720    1531    1984    2100    2343    2424    2697    2757 
   Size:        720     811     453     116     243      81     273      60

  Fragments arranged by size: 

                 811     720     453     273     243     116      81      60
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     154    2757 
   Size:        154    2603
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     313    2757 
   Size:        313    2444
 
BtsI GCAGTG_nn'

Cuts at:      0    1170    1838    2406    2411    2757 
   Size:       1170     668     568       5     346

  Fragments arranged by size: 

                1170     668     568     346       5
 
DraI TTT'AAA

Cuts at:      0    1962    2757 
   Size:       1962     795
 
DraIII CAC_nnn'GTG

Cuts at:      0    1678    2757 
   Size:       1678    1079
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     974    2050    2757 
   Size:        974    1076     707

  Fragments arranged by size: 

                1076     974     707
 
EarI CTCTTCn'nnn_

Cuts at:      0     491    1698    1757    2757 
   Size:        491    1207      59    1000

  Fragments arranged by size: 

                1207    1000     491      59
 
EcoICRI GAG'CTC

Cuts at:      0    2560    2757 
   Size:       2560     197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     121     178     280     668     727    1679    1684 
   Size:         29      92      57     102     388      59     952       5

Cuts at:   1684    1718    1871    2025    2210    2310    2576    2757 
   Size:         34     153     154     185     100     266     181

  Fragments arranged by size: 

                 952     388     266     185     181     154     153     102
                 100      92      59      57      34      29       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     103    2757 
   Size:        103    2654
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2238    2619    2757 
   Size:       2238     381     138

  Fragments arranged by size: 

                2238     381     138
 
EcoRI G'AATT_C

Cuts at:      0      66     520    2757 
   Size:         66     454    2237

  Fragments arranged by size: 

                2237     454      66
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     622     694    2757 
   Size:        622      72    2063

  Fragments arranged by size: 

                2063     622      72
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     414     446     918     950    1759    1791    2521    2553 
   Size:        414      32     472      32     809      32     730      32

Cuts at:   2553    2757 
   Size:        204

  Fragments arranged by size: 

                 809     730     472     414     204      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     291     807    2757 
   Size:        291     516    1950

  Fragments arranged by size: 

                1950     516     291
 
HindIII A'AGCT_T

Cuts at:      0      76     278    1444    2757 
   Size:         76     202    1166    1313

  Fragments arranged by size: 

                1313    1166     202      76
 
HpaI GTT'AAC

Cuts at:      0     291    2757 
   Size:        291    2466
 
MslI CAynn'nnrTG

Cuts at:      0      88    2525    2647    2757 
   Size:         88    2437     122     110

  Fragments arranged by size: 

                2437     122     110      88
 
MspA1I CmG'CkG

Cuts at:      0     913    1338    1849    2757 
   Size:        913     425     511     908

  Fragments arranged by size: 

                 913     908     511     425
 
NdeI CA'TA_TG

Cuts at:      0     837    2757 
   Size:        837    1920
 
NsiI A_TGCA'T

Cuts at:      0    2120    2757 
   Size:       2120     637
 
NspI r_CATG'y

Cuts at:      0     367    2475    2757 
   Size:        367    2108     282

  Fragments arranged by size: 

                2108     367     282
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     327    1723    2757 
   Size:        327    1396    1034

  Fragments arranged by size: 

                1396    1034     327
 
PfoI T'CCnGG_A

Cuts at:      0    1224    1635    2757 
   Size:       1224     411    1122

  Fragments arranged by size: 

                1224    1122     411
 
PpuMI rG'GwC_Cy

Cuts at:      0    2619    2757 
   Size:       2619     138
 
PshAI GACnn'nnGTC

Cuts at:      0    1580    2757 
   Size:       1580    1177
 
PstI C_TGCA'G

Cuts at:      0    2724    2757 
   Size:       2724      33
 
PvuII CAG'CTG

Cuts at:      0     913    1849    2757 
   Size:        913     936     908

  Fragments arranged by size: 

                 936     913     908
 
SacI G_AGCT'C

Cuts at:      0    2562    2757 
   Size:       2562     195
 
SfcI C'TryA_G

Cuts at:      0     399    2414    2420    2720    2757 
   Size:        399    2015       6     300      37

  Fragments arranged by size: 

                2015     399     300      37       6
 
SmlI C'TyrA_G

Cuts at:      0    1084    2757 
   Size:       1084    1673
 
SpeI A'CTAG_T

Cuts at:      0    1552    2757 
   Size:       1552    1205
 
SspI AAT'ATT

Cuts at:      0     798    1285    1461    1611    2757 
   Size:        798     487     176     150    1146

  Fragments arranged by size: 

                1146     798     487     176     150
 
StuI AGG'CCT

Cuts at:      0     602    1155    2757 
   Size:        602     553    1602

  Fragments arranged by size: 

                1602     602     553
 
StyI C'CwwG_G

Cuts at:      0     999    1476    2757 
   Size:        999     477    1281

  Fragments arranged by size: 

                1281     999     477
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1695    2707    2757 
   Size:       1695    1012      50

  Fragments arranged by size: 

                1695    1012      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     531     563    1998    2030    2757 
   Size:        531      32    1435      32     727

  Fragments arranged by size: 

                1435     727     531      32      32
 
XbaI T'CTAG_A

Cuts at:      0     210     369    2757 
   Size:        210     159    2388

  Fragments arranged by size: 

                2388     210     159
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1510    1995    2251    2757 
   Size:       1510     485     256     506

  Fragments arranged by size: 

                1510     506     485     256
 
XmnI GAAnn'nnTTC

Cuts at:      0     191     674    1274    2757 
   Size:        191     483     600    1483

  Fragments arranged by size: 

                1483     600     483     191
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI     AseI     BaeI    BamHI    BanII
     BbsI     BclI    BfrBI    BglII Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BssSI
    BstYI   Bsu36I    BtgZI     BtsI     DraI   DraIII     DrdI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII
  HindIII     HpaI     MslI   MspA1I     NdeI     NsiI     NspI    PflMI
     PfoI    PpuMI    PshAI     PstI    PvuII     SacI     SfcI     SmlI
     SpeI     SspI     StuI     StyI    TaqII     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI
     AvaI    AvrII     BanI     BbeI    BbvCI     BcgI    BciVI     BglI
     BlpI    BmgBI     BmtI     BsaI    BsaAI    BsaHI    BsaWI    BsiEI
    BsiWI    BspEI    BspMI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI     ClaI    CspCI     EaeI     EagI     EciI
    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI     TatI  Tth111I
     XhoI     XmaI     ZraI
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