Restriction map for pF1KSDA0883
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspHI       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvI        BcgI        BclI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsiEI       BsiHKAI     
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MslI        MunI        NarI        NciI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TseI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         847  CGCG                 CG^CG
AhdI          670  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          948  GGATC                GGATC(4/5)
AspEI         670  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          871  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          351  CTCGGG               C^[CT]CG[GA]G
BalI          583  TGGCCA               TGG^CCA
BanI          926  GGTGCC               G^G[CT][GA]CC
BbsI          765  GAAGAC               GAAGAC(2/6)
BbvCI         460  CCTCAGC              CCTCAGC(-5/-2)
BciVI          74  GGATAC               GTATCC(6/5)
BglI          626  GCCTGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         765  GAAGAC               GAAGAC(2/6)
BsmBI        1085  GAGACG               CGTCTC(1/5)
BsmI          180  GAATGC               GAATGC(1/-1)
BsoBI         351  CTCGGG               C^[CT]CG[GA]G
BspHI        1077  TCATGA               T^CATGA
BsrBI         833  GAGCGG               CCGCTC(-3/-3)
BsrDI         445  GCAATG               GCAATG(2/0)
BstAPI        654  GCACATAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         847  CGCG                 CG^CG
DraI          126  TTTAAA               TTT^AAA
DraIII        655  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          123  GACTTTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          563  CCACGG               C^C[GA][CT]GG
Eam1105I      670  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII           3  GGCGCT               [GA]GCGC^[CT]
MluNI         583  TGGCCA               TGG^CCA
MscI          583  TGGCCA               TGG^CCA
MspA1I       1068  CCGCTG               C[AC]G^C[GT]G
MvnI          847  CGCG                 CG^CG
NaeI          929  GCCGGC               GCC^GGC
NgoMIV        929  GCCGGC               G^CCGGC
NspI          453  ACATGC               [GA]CATG^[CT]
PflFI         871  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         429  CCATTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          535  GAGTC                GAGTC(4/5)
PpuMI         110  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1077  TCATGA               T^CATGA
SapI         1039  GAAGAGC              GCTCTTC(1/4)
SexAI         872  ACCAGGT              A^CC[AT]GGT
SmlI          973  CTTGAG               C^T[CT][GA]AG
Tsp45I         57  GTCAC                ^GT[GC]AC
Tsp509I       419  AATT                 ^AATT
Tth111I       871  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        429  CCATTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          327  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccIII        167  TCCGGA               T^CCGGA
              827  TCCGGA              
AlwNI         463  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              916  CAGATGCTG           
ApaI          633  GGGCCC               GGGCC^C
              954  GGGCCC              
BpmI          389  CTCCAG               CTGGAG(16/14)
              883  CTGGAG              
BsaI          348  GGTCTC               GGTCTC(1/5)
              792  GGTCTC              
BseAI         167  TCCGGA               T^CCGGA
              827  TCCGGA              
BseRI          91  GAGGAG               GAGGAG(10/8)
             1003  GAGGAG              
BsgI           27  GTGCAG               GTGCAG(16/14)
              661  GTGCAG              
BsmFI         438  GGGAC                GGGAC(10/14)
              517  GTCCC               
BspEI         167  TCCGGA               T^CCGGA
              827  TCCGGA              
BspMI         140  GCAGGT               ACCTGC(4/8)
              888  GCAGGT              
BsrFI          78  ACCGGC               [GA]^CCGG[CT]
              929  GCCGGC              
BstF5I        852  CATCC                GGATG(2/0)
               45  GGATG               
Cfr10I         78  ACCGGC               [GA]^CCGG[CT]
              929  GCCGGC              
DpnII         273  GATC                 ^GATC
              949  GATC                
EcoNI         369  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              882  CCTGGAGCAGG         
FauI          957  CCCGC                CCCGC(4/6)
              630  GCGGG               
Fnu4HI        737  GCCGC                GC^[ACGT]GC
             1067  GCCGC               
FokI          852  CATCC                GGATG(9/13)
               45  GGATG               
ItaI          737  GCCGC                GC^[ACGT]GC
             1067  GCCGC               
MboI          273  GATC                 ^GATC
              949  GATC                
MroI          167  TCCGGA               T^CCGGA
              827  TCCGGA              
NdeII         273  GATC                 ^GATC
              949  GATC                
Sau3AI        273  GATC                 ^GATC
              949  GATC                
SfaNI         918  GATGC                GCATC(5/9)
              407  GCATC               
StuI          704  AGGCCT               AGG^CCT
             1013  AGGCCT              
TaqI          363  TCGA                 T^CGA
             1037  TCGA                
TfiI          102  GATTC                G^A[AT]TC
             1074  GAATC               
TthHB8I       363  TCGA                 T^CGA
             1037  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BfaI        
BmyI        BsaJI       BsaWI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
EaeI        EarI        EcoO109I    EcoRII      HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        Ksp632I     MaeI        MaeIII      MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NlaIII      NlaIV       Sau96I      ScrFI       Tru9I       
TspRI       
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