Restriction map for pF1KSDA0873
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsgI        BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       StuI        
SwaI        Tsp45I      Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflIII        405  ACATGT               A^C[GA][CT]GT
AhdI          222  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       226  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         222  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1290  TGCGCA               TGC^GCA
BanII         912  GAGCTC               G[GA]GC[CT]^C
BclI         1025  TGATCA               T^GATCA
BcnI          984  CCGGG                CC^[GC]GG
BsaI          631  GAGACC               GGTCTC(1/5)
BsaWI         269  ACCGGA               [AT]^CCGG[AT]
BseRI        1081  GAGGAG               GAGGAG(10/8)
BsiEI         699  CGGCCG               CG[GA][CT]^CG
BsiYI         977  CCTGCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          977  CCTGCATCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         306  GTCCC                GGGAC(10/14)
BsmI          252  GAATGC               GAATGC(1/-1)
BspLU11I      405  ACATGT               A^CATGT
BspMI         634  ACCTGC               ACCTGC(4/8)
BssSI         310  CTCGTG               CACGAG(-5/-1)
BstYI        1133  AGATCC               [GA]^GATC[CT]
CfoI         1291  GCGC                 GCG^C
DraI          828  TTTAAA               TTT^AAA
EaeI          699  CGGCCG               [CT]^GGCC[GA]
EagI          699  CGGCCG               C^GGCCG
Eam1105I      222  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         699  CGGCCG               C^GGCCG
Eco52I        699  CGGCCG               C^GGCCG
EcoT14I      1116  CCTTGG               C^C[AT][AT]GG
FauI          207  GCGGG                CCCGC(4/6)
FbaI         1025  TGATCA               T^GATCA
FspI         1290  TGCGCA               TGC^GCA
HhaI         1291  GCGC                 GCG^C
HinP1I       1291  GCGC                 G^CGC
MaeIII        355  GTAAC                ^GT[ACGT]AC
MflI         1133  AGATCC               [GA]^GATC[CT]
MspA1I       1213  CAGCTG               C[AC]G^C[GT]G
NciI          984  CCGGG                CC^[GC]GG
NheI         1218  GCTAGC               G^CTAGC
NlaIV         593  GGGTCC               GG[ACGT]^[ACGT]CC
NspI          405  ACATGT               [GA]CATG^[CT]
PleI          160  GACTC                GAGTC(4/5)
PstI          636  CTGCAG               CTGCA^G
PvuII        1213  CAGCTG               CAG^CTG
SacI          912  GAGCTC               GAGCT^C
SbfI          635  CCTGCAGG             CCTGCA^GG
Sse8387I      635  CCTGCAGG             CCTGCA^GG
SspI         1297  AATATT               AAT^ATT
StyI         1116  CCTTGG               C^C[AT][AT]GG
XbaI          687  TCTAGA               T^CTAGA
XhoII        1133  AGATCC               [GA]^GATC[CT]
XmnI          226  GAACGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          906  GTAC                 GT^AC
             1338  GTAC                
AlwI         1134  GATCC                GGATC(4/5)
             1237  GATCC               
AvaI          587  CCCGAG               C^[CT]CG[GA]G
              660  CTCGGG              
AvaII         594  GGTCC                G^G[AT]CC
              843  GGTCC               
BbsI          490  GAAGAC               GAAGAC(2/6)
              230  GTCTTC              
BpmI          124  CTGGAG               CTGGAG(16/14)
              416  CTGGAG              
BpuAI         490  GAAGAC               GAAGAC(2/6)
              230  GTCTTC              
BsaBI         532  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1159  GATGGAAATC          
BsoBI         587  CCCGAG               C^[CT]CG[GA]G
              660  CTCGGG              
EcoRV         385  GATATC               GAT^ATC
              742  GATATC              
HphI          576  GGTGA                GGTGA(8/7)
             1205  TCACC               
MaeII         228  ACGT                 A^CGT
             1112  ACGT                
MamI          532  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1159  GATGGAAATC          
NlaIII        406  CATG                 CATG^
              498  CATG                
PpuMI         593  GGGTCCT              [GA]G^G[AT]CC[CT]
              842  AGGTCCT             
RsaI          906  GTAC                 GT^AC
             1338  GTAC                
SapI          998  GAAGAGC              GCTCTTC(1/4)
             1195  GAAGAGC             
SfcI          164  CTGTAG               C^T[GA][CT]AG
              636  CTGCAG              
SmlI          847  CTCAAG               C^T[CT][GA]AG
             1054  CTTGAG              
TfiI          132  GATTC                G^A[AT]TC
              816  GAATC               
TspRI          90  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1004  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AspHI       BbvI        BfaI        
BmyI        BsaJI       BsiHKAI     BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        Ksp632I     
MaeI        MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        Tru9I       
TseI        Tsp509I     TthHB8I     
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