Restriction map for pF1KSDA0873
 (Linear) (Six-base) MAPSORT of: KIAA0873.seq  Check: 9770  from: 1  to: 1341



KIAA0873.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      72     194    1341 
   Size:         22      50     122    1147

  Fragments arranged by size: 

                1147     122      50      22
 
AflIII A'CryG_T

Cuts at:      0     405    1341 
   Size:        405     936
 
AhdI GACnn_n'nnGTC

Cuts at:      0     227    1341 
   Size:        227    1114
 
AvaI C'yCGr_G

Cuts at:      0     587     660    1341 
   Size:        587      73     681

  Fragments arranged by size: 

                 681     587      73
 
BanII G_rGCy'C

Cuts at:      0     916    1341 
   Size:        916     425
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     223     497    1341 
   Size:        223     274     844

  Fragments arranged by size: 

                 844     274     223
 
BclI T'GATC_A

Cuts at:      0    1025    1341 
   Size:       1025     316
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     297    1341 
   Size:        297    1044
 
BmtI G_CTAG'C

Cuts at:      0    1222    1341 
   Size:       1222     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     437    1341 
   Size:        145     292     904

  Fragments arranged by size: 

                 904     292     145
 
Bpu10I CC'TnA_GC

Cuts at:      0     910    1341 
   Size:        910     431
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1075    1341 
   Size:        832     243     266

  Fragments arranged by size: 

                 832     266     243
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     625    1341 
   Size:        625     716
 
BsaBI GATnn'nnATC

Cuts at:      0     536    1163    1341 
   Size:        536     627     178

  Fragments arranged by size: 

                 627     536     178
 
BsaWI w'CCGG_w

Cuts at:      0     269    1341 
   Size:        269    1072
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     898     928    1341 
   Size:        898      30     413

  Fragments arranged by size: 

                 898     413      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1096    1341 
   Size:       1096     245
 
BsiEI CG_ry'CG

Cuts at:      0     702    1341 
   Size:        702     639
 
BsiHKAI G_wGCw'C

Cuts at:      0     659     916    1088    1341 
   Size:        659     257     172     253

  Fragments arranged by size: 

                 659     257     253     172
 
BsmI GAATG_Cn'

Cuts at:      0     258    1341 
   Size:        258    1083
 
Bsp1286I G_dGCh'C

Cuts at:      0     659     916    1088    1341 
   Size:        659     257     172     253

  Fragments arranged by size: 

                 659     257     253     172
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     643    1341 
   Size:        643     698
 
BssSI C'ACGA_G

Cuts at:      0     310    1341 
   Size:        310    1031
 
BstYI r'GATC_y

Cuts at:      0    1133    1341 
   Size:       1133     208
 
BtsI GCAGTG_nn'

Cuts at:      0    1010    1341 
   Size:       1010     331
 
DraI TTT'AAA

Cuts at:      0     830    1341 
   Size:        830     511
 
EaeI y'GGCC_r

Cuts at:      0     699    1341 
   Size:        699     642
 
EagI C'GGCC_G

Cuts at:      0     699    1341 
   Size:        699     642
 
EarI CTCTTCn'nnn_

Cuts at:      0     770     993    1190    1244    1341 
   Size:        770     223     197      54      97

  Fragments arranged by size: 

                 770     223     197      97      54
 
EcoICRI GAG'CTC

Cuts at:      0     914    1341 
   Size:        914     427
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      72     145     194     437    1341 
   Size:         22      50      73      49     243     904

  Fragments arranged by size: 

                 904     243      73      50      49      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     594     843    1050    1341 
   Size:        594     249     207     291

  Fragments arranged by size: 

                 594     291     249     207
 
EcoRV GAT'ATC

Cuts at:      0     387     744    1341 
   Size:        387     357     597

  Fragments arranged by size: 

                 597     387     357
 
FspI TGC'GCA

Cuts at:      0    1292    1341 
   Size:       1292      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245     410     442     801     833     992    1024 
   Size:        213      32     165      32     359      32     159      32

Cuts at:   1024    1075    1107    1306    1338    1341 
   Size:         51      32     199      32       3

  Fragments arranged by size: 

                 359     213     199     165     159      51      32      32
                  32      32      32      32       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     929    1341 
   Size:        224     705     412

  Fragments arranged by size: 

                 705     412     224
 
MspA1I CmG'CkG

Cuts at:      0    1215    1341 
   Size:       1215     126
 
NheI G'CTAG_C

Cuts at:      0    1218    1341 
   Size:       1218     123
 
NspI r_CATG'y

Cuts at:      0     409    1341 
   Size:        409     932
 
PciI A'CATG_T

Cuts at:      0     405    1341 
   Size:        405     936
 
PfoI T'CCnGG_A

Cuts at:      0     983    1341 
   Size:        983     358
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     218     250    1341 
   Size:        218      32    1091

  Fragments arranged by size: 

                1091     218      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     594     843    1341 
   Size:        594     249     498

  Fragments arranged by size: 

                 594     498     249
 
PsiI TTA'TAA

Cuts at:      0     338    1341 
   Size:        338    1003
 
PstI C_TGCA'G

Cuts at:      0     640    1341 
   Size:        640     701
 
PvuII CAG'CTG

Cuts at:      0    1215    1341 
   Size:       1215     126
 
SacI G_AGCT'C

Cuts at:      0     916    1341 
   Size:        916     425
 
SapI GCTCTTCn'nnn_

Cuts at:      0     993    1190    1341 
   Size:        993     197     151

  Fragments arranged by size: 

                 993     197     151
 
SbfI CC_TGCA'GG

Cuts at:      0     640    1341 
   Size:        640     701
 
SfcI C'TryA_G

Cuts at:      0     164     636    1341 
   Size:        164     472     705

  Fragments arranged by size: 

                 705     472     164
 
SmlI C'TyrA_G

Cuts at:      0     847    1054    1341 
   Size:        847     207     287

  Fragments arranged by size: 

                 847     287     207
 
SspI AAT'ATT

Cuts at:      0    1299    1341 
   Size:       1299      42
 
StyI C'CwwG_G

Cuts at:      0    1116    1341 
   Size:       1116     225
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     604    1103    1341 
   Size:        278     326     499     238

  Fragments arranged by size: 

                 499     326     278     238
 
XbaI T'CTAG_A

Cuts at:      0     687    1341 
   Size:        687     654
 
XmnI GAAnn'nnTTC

Cuts at:      0     230    1341 
   Size:        230    1111
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AvaI    BanII     BbsI     BclI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI    BstYI
     BtsI     DraI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FspI    Hin4I     MmeI   MspA1I     NheI     NspI     PciI
     PfoI     PpiI    PpuMI     PsiI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SmlI     SspI     StyI    TaqII     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BplI    BsaAI    BsaHI    BseYI     BsgI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EciI    EcoNI    EcoRI     FalI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     ZraI
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