Restriction map for
pF1KE2503
(Linear) (Six-base) MAPSORT of: KIEE2503.seq Check: 5758 from: 1 to: 1893
KIEE2503.orf
With 182 enzymes: SgfI *
February 16, 2012 13:36 ..
AccI GT'mk_AC
Cuts at: 0 342 1893
Size: 342 1551
AclI AA'CG_TT
Cuts at: 0 573 1893
Size: 573 1320
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1093 1135 1210 1893
Size: 112 981 42 75 683
Fragments arranged by size:
981 683 112 75 42
AflIII A'CryG_T
Cuts at: 0 726 1893
Size: 726 1167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 1893
Size: 310 32 1551
Fragments arranged by size:
1551 310 32
AlwNI CAG_nnn'CTG
Cuts at: 0 46 714 1158 1321 1844 1893
Size: 46 668 444 163 523 49
Fragments arranged by size:
668 523 444 163 49 46
ApaI G_GGCC'C
Cuts at: 0 810 1893
Size: 810 1083
ApoI r'AATT_y
Cuts at: 0 1603 1893
Size: 1603 290
AvaI C'yCGr_G
Cuts at: 0 321 654 1669 1893
Size: 321 333 1015 224
Fragments arranged by size:
1015 333 321 224
BanI G'GyrC_C
Cuts at: 0 178 1893
Size: 178 1715
BanII G_rGCy'C
Cuts at: 0 40 292 810 1025 1653 1670 1893
Size: 40 252 518 215 628 17 223
Fragments arranged by size:
628 518 252 223 215 40 17
BlpI GC'TnA_GC
Cuts at: 0 935 1893
Size: 935 958
Bme1580I G_kGCm'C
Cuts at: 0 183 810 1598 1893
Size: 183 627 788 295
Fragments arranged by size:
788 627 295 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 804 1331 1634 1893
Size: 184 620 527 303 259
Fragments arranged by size:
620 527 303 259 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 787 919 1009 1024 1375 1568 1893
Size: 121 666 132 90 15 351 193 325
Fragments arranged by size:
666 351 325 193 132 121 90 15
Bpu10I CC'TnA_GC
Cuts at: 0 1031 1893
Size: 1031 862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1893
Size: 661 1232
BsaI GGTCTCn'nnnn_
Cuts at: 0 611 1893
Size: 611 1282
BsaAI yAC'GTr
Cuts at: 0 729 1893
Size: 729 1164
BsaBI GATnn'nnATC
Cuts at: 0 881 1893
Size: 881 1012
BsaWI w'CCGG_w
Cuts at: 0 1415 1893
Size: 1415 478
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 114 144 1550 1580 1893
Size: 114 30 1406 30 313
Fragments arranged by size:
1406 313 114 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 246 255 258 877 907 918 1114
Size: 148 98 9 3 619 30 11 196
Cuts at: 1114 1198 1372 1508 1541 1893
Size: 84 174 136 33 352
Fragments arranged by size:
619 352 196 174 148 136 98 84
33 30 11 9 3
BseYI C'CCAG_C
Cuts at: 0 214 957 1251 1308 1653 1865 1893
Size: 214 743 294 57 345 212 28
Fragments arranged by size:
743 345 294 214 212 57 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 147 1715 1760 1810 1893
Size: 92 55 1568 45 50 83
Fragments arranged by size:
1568 92 83 55 50 45
BsiEI CG_ry'CG
Cuts at: 0 564 1283 1893
Size: 564 719 610
Fragments arranged by size:
719 610 564
BsiHKAI G_wGCw'C
Cuts at: 0 1025 1670 1826 1893
Size: 1025 645 156 67
Fragments arranged by size:
1025 645 156 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1261 1893
Size: 1261 632
Bsp1286I G_dGCh'C
Cuts at: 0 40 183 292 810 1025 1598 1653 1670
Size: 40 143 109 518 215 573 55 17
Cuts at: 1670 1826 1893
Size: 156 67
Fragments arranged by size:
573 518 215 156 143 109 67 55
40 17
BsrFI r'CCGG_y
Cuts at: 0 559 1893
Size: 559 1334
BssSI C'ACGA_G
Cuts at: 0 1195 1893
Size: 1195 698
BstAPI GCAn_nnn'nTGC
Cuts at: 0 714 1844 1893
Size: 714 1130 49
Fragments arranged by size:
1130 714 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 555 1722 1893
Size: 555 1167 171
Fragments arranged by size:
1167 555 171
BstYI r'GATC_y
Cuts at: 0 96 552 1893
Size: 96 456 1341
Fragments arranged by size:
1341 456 96
Bsu36I CC'TnA_GG
Cuts at: 0 991 1314 1372 1893
Size: 991 323 58 521
Fragments arranged by size:
991 521 323 58
BtgI C'CryG_G
Cuts at: 0 1068 1893
Size: 1068 825
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 563 1429 1893
Size: 563 866 464
Fragments arranged by size:
866 563 464
BtsI GCAGTG_nn'
Cuts at: 0 135 940 968 1210 1893
Size: 135 805 28 242 683
Fragments arranged by size:
805 683 242 135 28
EaeI y'GGCC_r
Cuts at: 0 557 561 1160 1280 1610 1893
Size: 557 4 599 120 330 283
Fragments arranged by size:
599 557 330 283 120 4
EagI C'GGCC_G
Cuts at: 0 561 1280 1893
Size: 561 719 613
Fragments arranged by size:
719 613 561
EarI CTCTTCn'nnn_
Cuts at: 0 536 1138 1893
Size: 536 602 755
Fragments arranged by size:
755 602 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 249 1381 1893
Size: 135 114 1132 512
Fragments arranged by size:
1132 512 135 114
EcoICRI GAG'CTC
Cuts at: 0 1023 1668 1893
Size: 1023 645 225
Fragments arranged by size:
1023 645 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 121 787 919 1009 1024 1093 1135
Size: 112 9 666 132 90 15 69 42
Cuts at: 1135 1210 1375 1568 1893
Size: 75 165 193 325
Fragments arranged by size:
666 325 193 165 132 112 90 75
69 42 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 994 1354 1733 1893
Size: 994 360 379 160
Fragments arranged by size:
994 379 360 160
EcoO109I rG'GnC_Cy
Cuts at: 0 806 1893
Size: 806 1087
FseI GG_CCGG'CC
Cuts at: 0 563 1893
Size: 563 1330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 637 669 1416 1448 1893
Size: 46 32 559 32 747 32 445
Fragments arranged by size:
747 559 445 46 32 32 32
HincII GTy'rAC
Cuts at: 0 343 1893
Size: 343 1550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 176 1199 1893
Size: 11 165 1023 694
Fragments arranged by size:
1023 694 165 11
MslI CAynn'nnrTG
Cuts at: 0 66 1893
Size: 66 1827
MspA1I CmG'CkG
Cuts at: 0 168 193 399 765 1158 1615 1893
Size: 168 25 206 366 393 457 278
Fragments arranged by size:
457 393 366 278 206 168 25
NaeI GCC'GGC
Cuts at: 0 561 1893
Size: 561 1332
NgoMIV G'CCGG_C
Cuts at: 0 559 1893
Size: 559 1334
NruI TCG'CGA
Cuts at: 0 1091 1893
Size: 1091 802
NspI r_CATG'y
Cuts at: 0 280 1384 1893
Size: 280 1104 509
Fragments arranged by size:
1104 509 280
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 1263 1893
Size: 13 1250 630
Fragments arranged by size:
1250 630 13
PfoI T'CCnGG_A
Cuts at: 0 1352 1644 1893
Size: 1352 292 249
Fragments arranged by size:
1352 292 249
PmlI CAC'GTG
Cuts at: 0 729 1893
Size: 729 1164
PspOMI G'GGCC_C
Cuts at: 0 806 1893
Size: 806 1087
PspXI vC'TCGA_Gb
Cuts at: 0 321 1893
Size: 321 1572
PstI C_TGCA'G
Cuts at: 0 1217 1244 1557 1893
Size: 1217 27 313 336
Fragments arranged by size:
1217 336 313 27
PvuII CAG'CTG
Cuts at: 0 193 399 765 1158 1893
Size: 193 206 366 393 735
Fragments arranged by size:
735 393 366 206 193
SacI G_AGCT'C
Cuts at: 0 1025 1670 1893
Size: 1025 645 223
Fragments arranged by size:
1025 645 223
SalI G'TCGA_C
Cuts at: 0 341 1893
Size: 341 1552
SfcI C'TryA_G
Cuts at: 0 1213 1240 1553 1893
Size: 1213 27 313 340
Fragments arranged by size:
1213 340 313 27
SmlI C'TyrA_G
Cuts at: 0 321 654 676 1893
Size: 321 333 22 1217
Fragments arranged by size:
1217 333 321 22
SphI G_CATG'C
Cuts at: 0 280 1893
Size: 280 1613
StyI C'CwwG_G
Cuts at: 0 548 1797 1893
Size: 548 1249 96
Fragments arranged by size:
1249 548 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 1841 1893
Size: 166 1675 52
Fragments arranged by size:
1675 166 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 114 146 1893
Size: 114 32 1747
Fragments arranged by size:
1747 114 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1507 1862 1893
Size: 1507 355 31
Fragments arranged by size:
1507 355 31
XhoI C'TCGA_G
Cuts at: 0 321 654 1893
Size: 321 333 1239
Fragments arranged by size:
1239 333 321
Enzymes that do cut:
AccI AclI AcuI AflIII AloI AlwNI ApaI ApoI
AvaI BanI BanII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI Hin4I
HincII MmeI MslI MspA1I NaeI NgoMIV NruI NspI
PflMI PfoI PmlI PspOMI PspXI PstI PvuII SacI
SalI SfcI SmlI SphI StyI TaqII TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BsaHI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EcoRI EcoRV
FalI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PsrI PvuI RsrII SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI
XmaI XmnI ZraI