Restriction map for
pF1KSDA0854
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbvCI BfrI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmI
BspDI BspEI BspLU11I BspMI BsrDI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoRI FseI FspI Hin1I KasI
KpnI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1539 CGCG CG^CG
AgeI 2309 ACCGGT A^CCGGT
AocI 1926 CCTGAGG CC^T[ACGT]AGG
Aor51HI 938 AGCGCT AGC^GCT
BbrPI 631 CACGTG CAC^GTG
BcgI 1509 CGAATCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 813 GGATAC GTATCC(6/5)
BlpI 1701 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1701 GCTGAGC GC^T[ACGT]AGC
BsgI 335 GTGCAG GTGCAG(16/14)
BsmBI 1161 CGTCTC CGTCTC(1/5)
Bsp1407I 735 TGTACA T^GTACA
BspHI 557 TCATGA T^CATGA
BsrBI 1309 CCGCTC CCGCTC(-3/-3)
BsrGI 735 TGTACA T^GTACA
BstAPI 239 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1539 CGCG CG^CG
Bsu36I 1926 CCTGAGG CC^T[ACGT]AGG
CelII 1701 GCTGAGC GC^T[ACGT]AGC
CfoI 939 GCGC GCG^C
DrdI 1143 GACCAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 938 AGCGCT AGC^GCT
Eco81I 1926 CCTGAGG CC^T[ACGT]AGG
EcoRV 1472 GATATC GAT^ATC
EcoT22I 30 ATGCAT ATGCA^T
HaeII 938 AGCGCT [GA]GCGC^[CT]
HhaI 939 GCGC GCG^C
HinP1I 939 GCGC G^CGC
HindIII 1744 AAGCTT A^AGCTT
HpaI 1863 GTTAAC GTT^AAC
MfeI 1895 CAATTG C^AATTG
MunI 1895 CAATTG C^AATTG
MvnI 1539 CGCG CG^CG
NheI 4 GCTAGC G^CTAGC
NsiI 30 ATGCAT ATGCA^T
PacI 427 TTAATTAA TTAAT^TAA
PinAI 2309 ACCGGT A^CCGGT
PmaCI 631 CACGTG CAC^GTG
PmlI 631 CACGTG CAC^GTG
PstI 2492 CTGCAG CTGCA^G
RcaI 557 TCATGA T^CATGA
SacI 697 GAGCTC GAGCT^C
SalI 289 GTCGAC G^TCGAC
SfcI 2492 CTGCAG C^T[GA][CT]AG
SfiI 1533 GGCCATCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 400 CCCGGG CCC^GGG
SspBI 735 TGTACA T^GTACA
SspI 2186 AATATT AAT^ATT
XmaCI 400 CCCGGG C^CCGGG
XmaI 400 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 79 GTAGAC GT^[AC][GT]AC
289 GTCGAC
AflIII 329 ACGTGT A^C[GA][CT]GT
720 ACACGT
AlwNI 918 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2495 CAGAGGCTG
AspI 1550 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2482 GACTGCGTC
BbsI 1335 GAAGAC GAAGAC(2/6)
1633 GAAGAC
BclI 830 TGATCA T^GATCA
2103 TGATCA
BfaI 5 CTAG C^TAG
1121 CTAG
BpuAI 1335 GAAGAC GAAGAC(2/6)
1633 GAAGAC
BsaI 1218 GAGACC GGTCTC(1/5)
1693 GAGACC
BsaWI 1852 TCCGGT [AT]^CCGG[AT]
2309 ACCGGT
BsiEI 1554 CGGTCG CG[GA][CT]^CG
2390 CGGTCG
BsrFI 1409 ACCGGC [GA]^CCGG[CT]
2309 ACCGGT
BstEII 1140 GGTGACC G^GT[ACGT]ACC
2235 GGTGACC
BstPI 1140 GGTGACC G^GT[ACGT]ACC
2235 GGTGACC
Cfr10I 1409 ACCGGC [GA]^CCGG[CT]
2309 ACCGGT
DraII 770 AGGTCCC [GA]G^G[ACGT]CC[CT]
2195 AGGACCC
EcoO109I 770 AGGTCCC [GA]G^G[ACGT]CC[CT]
2195 AGGACCC
EcoO65I 1140 GGTGACC G^GT[ACGT]ACC
2235 GGTGACC
FbaI 830 TGATCA T^GATCA
2103 TGATCA
MaeI 5 CTAG C^TAG
1121 CTAG
NspI 285 GCATGT [GA]CATG^[CT]
293 ACATGC
PflFI 1550 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2482 GACTGCGTC
PflMI 1803 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2088 CCAGCCCATGG
PpuMI 770 AGGTCCC [GA]G^G[AT]CC[CT]
2195 AGGACCC
SmlI 678 CTCAAG C^T[CT][GA]AG
1360 CTCAAG
Tsp509I 429 AATT ^AATT
1896 AATT
Tth111I 1550 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2482 GACTGCGTC
Van91I 1803 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2088 CCAGCCCATGG
XcmI 1520 CCAAAGACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1988 CCAAGACTGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII XmnI