Restriction map for pF1KSDA0843
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MslI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccII         106  CGCG                 CG^CG
AflIII       1004  ACATGT               A^C[GA][CT]GT
Alw44I        109  GTGCAC               G^TGCAC
ApaI         1204  GGGCCC               GGGCC^C
ApaLI         109  GTGCAC               G^TGCAC
Asp700I       336  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          410  TGGCCA               TGG^CCA
BamHI         853  GGATCC               G^GATCC
BbsI         1387  GAAGAC               GAAGAC(2/6)
BfaI            8  CTAG                 C^TAG
BglI         1148  GCCGGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1856  AGATCT               A^GATCT
BpuAI        1387  GAAGAC               GAAGAC(2/6)
BsaWI        1322  ACCGGA               [AT]^CCGG[AT]
BsmBI        1104  GAGACG               CGTCTC(1/5)
BsmI           11  GCATTC               GAATGC(1/-1)
BspLU11I     1004  ACATGT               A^CATGT
BstAPI        323  GCAGCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         106  CGCG                 CG^CG
BstXI        1965  CCAAGTCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
HaeII        1937  AGCGCC               [GA]GCGC^[CT]
HgaI          545  GCGTC                GACGC(5/10)
HincII       1831  GTCAAC               GT[CT]^[GA]AC
HindII       1831  GTCAAC               GT[CT]^[GA]AC
MaeI            8  CTAG                 C^TAG
MluNI         410  TGGCCA               TGG^CCA
MscI          410  TGGCCA               TGG^CCA
MvnI          106  CGCG                 CG^CG
PflMI         402  CCAGTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          540  CTGCAG               CTGCA^G
ScaI         1457  AGTACT               AGT^ACT
TaqI         1603  TCGA                 T^CGA
TthHB8I      1603  TCGA                 T^CGA
Van91I        402  CCAGTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          336  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         1359  GTCTAC               GT^[AC][GT]AC
             1918  GTAGAC              
BanI          235  GGCACC               G^G[CT][GA]CC
              770  GGCACC              
BcgI          600  CGACTACCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              877  CGAGTCATCTGC        
BsiEI        1600  CGGTCG               CG[GA][CT]^CG
             1705  CGGTCG              
BspMI          85  ACCTGC               ACCTGC(4/8)
              538  ACCTGC              
BsrBI        1176  CCGCTC               CCGCTC(-3/-3)
             1197  CCGCTC              
BstEII        360  GGTGACC              G^GT[ACGT]ACC
              866  GGTCACC             
BstPI         360  GGTGACC              G^GT[ACGT]ACC
              866  GGTCACC             
DdeI         1454  CTGAG                C^T[ACGT]AG
             1988  CTGAG               
EcoNI         695  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1944  CCTGTCCCAGG         
EcoO65I       360  GGTGACC              G^GT[ACGT]ACC
              866  GGTCACC             
FauI          239  CCCGC                CCCGC(4/6)
             1175  CCCGC               
MaeII         394  ACGT                 A^CGT
              515  ACGT                
NaeI         1148  GCCGGC               GCC^GGC
             1568  GCCGGC              
NcoI          407  CCATGG               C^CATGG
             1470  CCATGG              
NgoMIV       1148  GCCGGC               G^CCGGC
             1568  GCCGGC              
SfaNI         134  GATGC                GCATC(5/9)
             1017  GATGC               
SmaI          794  CCCGGG               CCC^GGG
             1119  CCCGGG              
SphI         1541  GCATGC               GCATG^C
             1841  GCATGC              
Tsp509I       913  AATT                 ^AATT
             1563  AATT                
XmaCI         794  CCCGGG               C^CCGGG
             1119  CCCGGG              
XmaI          794  CCCGGG               C^CCGGG
             1119  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com