Restriction map for pF1KSDA0843
 (Linear) (Six-base) MAPSORT of: KIAA0843.seq  Check: 9720  from: 1  to: 2049



KIAA0843.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      94    2049 
   Size:         94    1955
 
AccI GT'mk_AC

Cuts at:      0    1360    1919    2049 
   Size:       1360     559     130

  Fragments arranged by size: 

                1360     559     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351    1600    2047    2049 
   Size:        351    1249     447       2

  Fragments arranged by size: 

                1249     447     351       2
 
AflIII A'CryG_T

Cuts at:      0    1004    2049 
   Size:       1004    1045
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1044    1076    2049 
   Size:       1044      32     973

  Fragments arranged by size: 

                1044     973      32
 
ApaI G_GGCC'C

Cuts at:      0    1208    2049 
   Size:       1208     841
 
ApaLI G'TGCA_C

Cuts at:      0     109    2049 
   Size:        109    1940
 
AvaI C'yCGr_G

Cuts at:      0     794    1119    1207    1490    2049 
   Size:        794     325      88     283     559

  Fragments arranged by size: 

                 794     559     325     283      88
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     636     669    2049 
   Size:        636      33    1380

  Fragments arranged by size: 

                1380     636      33
 
BamHI G'GATC_C

Cuts at:      0     853    2049 
   Size:        853    1196
 
BanI G'GyrC_C

Cuts at:      0     235     770    2049 
   Size:        235     535    1279

  Fragments arranged by size: 

                1279     535     235
 
BanII G_rGCy'C

Cuts at:      0    1208    1225    1609    1642    2049 
   Size:       1208      17     384      33     407

  Fragments arranged by size: 

                1208     407     384      33      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1394    2049 
   Size:       1394     655
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     589     623     866     900    2049 
   Size:        589      34     243      34    1149

  Fragments arranged by size: 

                1149     589     243      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1154    2049 
   Size:       1154     895
 
BglII A'GATC_T

Cuts at:      0    1856    2049 
   Size:       1856     193
 
Bme1580I G_kGCm'C

Cuts at:      0     113    1208    1490    2049 
   Size:        113    1095     282     559

  Fragments arranged by size: 

                1095     559     282     113
 
BmgBI CAC'GTC

Cuts at:      0     516    2049 
   Size:        516    1533
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     165     396     606    1845    2049 
   Size:        165     231     210    1239     204

  Fragments arranged by size: 

                1239     231     210     204     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1042    1098    1325    1532    2049 
   Size:       1042      56     227     207     517

  Fragments arranged by size: 

                1042     517     227     207      56
 
BsaWI w'CCGG_w

Cuts at:      0    1322    2049 
   Size:       1322     727
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     344     374    1044    1074    1117    1147    1441    1471 
   Size:        344      30     670      30      43      30     294      30

Cuts at:   1471    2049 
   Size:        578

  Fragments arranged by size: 

                 670     578     344     294      43      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     481    1529    1534    2049 
   Size:        481    1048       5     515

  Fragments arranged by size: 

                1048     515     481       5
 
BseYI C'CCAG_C

Cuts at:      0    1047    1299    1792    2049 
   Size:       1047     252     493     257

  Fragments arranged by size: 

                1047     493     257     252
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     342     351    1648    1765    2049 
   Size:        192     150       9    1297     117     284

  Fragments arranged by size: 

                1297     284     192     150     117       9
 
BsiEI CG_ry'CG

Cuts at:      0    1603    1708    2049 
   Size:       1603     105     341

  Fragments arranged by size: 

                1603     341     105
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     400    1225    1609    1642    2049 
   Size:        113     287     825     384      33     407

  Fragments arranged by size: 

                 825     407     384     287     113      33
 
BsmI GAATG_Cn'

Cuts at:      0      11    2049 
   Size:         11    2038
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1098    2049 
   Size:       1098     951
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     400    1208    1225    1490    1609    1642    2049 
   Size:        113     287     808      17     265     119      33     407

  Fragments arranged by size: 

                 808     407     287     265     119     113      33      17
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     547    2049 
   Size:         94     453    1502

  Fragments arranged by size: 

                1502     453      94
 
BsrBI CCG'CTC

Cuts at:      0    1178    1199    2049 
   Size:       1178      21     850

  Fragments arranged by size: 

                1178     850      21
 
BsrFI r'CCGG_y

Cuts at:      0    1148    1568    1997    2049 
   Size:       1148     420     429      52

  Fragments arranged by size: 

                1148     429     420      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     329    2049 
   Size:        329    1720
 
BstEII G'GTnAC_C

Cuts at:      0     360     866    2049 
   Size:        360     506    1183

  Fragments arranged by size: 

                1183     506     360
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1972    2049 
   Size:       1972      77
 
BstYI r'GATC_y

Cuts at:      0     853     905    1617    1856    2049 
   Size:        853      52     712     239     193

  Fragments arranged by size: 

                 853     712     239     193      52
 
BtgI C'CryG_G

Cuts at:      0      40     407    1470    2049 
   Size:         40     367    1063     579

  Fragments arranged by size: 

                1063     579     367      40
 
BtsI GCAGTG_nn'

Cuts at:      0     514    2049 
   Size:        514    1535
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     417     452    2049 
   Size:        417      35    1597

  Fragments arranged by size: 

                1597     417      35
 
EaeI y'GGCC_r

Cuts at:      0     410     480    1215    1566    2049 
   Size:        410      70     735     351     483

  Fragments arranged by size: 

                 735     483     410     351      70
 
EarI CTCTTCn'nnn_

Cuts at:      0    1810    1949    2008    2049 
   Size:       1810     139      59      41

  Fragments arranged by size: 

                1810     139      59      41
 
EcoICRI GAG'CTC

Cuts at:      0    1223    1607    1640    2049 
   Size:       1223     384      33     409

  Fragments arranged by size: 

                1223     409     384      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351    1042    1098    1325    1532    1600    2047    2049 
   Size:        351     691      56     227     207      68     447       2

  Fragments arranged by size: 

                 691     447     351     227     207      68      56       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     699    1948    2049 
   Size:        699    1249     101

  Fragments arranged by size: 

                1249     699     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1253    1475    1925    2049 
   Size:       1253     222     450     124

  Fragments arranged by size: 

                1253     450     222     124
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     381    2049 
   Size:        381    1668
 
HaeII r_GCGC'y

Cuts at:      0    1941    2049 
   Size:       1941     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1237    1269    2049 
   Size:       1237      32     780

  Fragments arranged by size: 

                1237     780      32
 
HincII GTy'rAC

Cuts at:      0    1833    2049 
   Size:       1833     216
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    1034    1288    2049 
   Size:        623     411     254     761

  Fragments arranged by size: 

                 761     623     411     254
 
MscI TGG'CCA

Cuts at:      0     412    2049 
   Size:        412    1637
 
MspA1I CmG'CkG

Cuts at:      0      74     242     251     371     521    1303    1672    2049 
   Size:         74     168       9     120     150     782     369     377

  Fragments arranged by size: 

                 782     377     369     168     150     120      74       9
            
 
NaeI GCC'GGC

Cuts at:      0    1150    1570    2049 
   Size:       1150     420     479

  Fragments arranged by size: 

                1150     479     420
 
NcoI C'CATG_G

Cuts at:      0     407    1470    2049 
   Size:        407    1063     579

  Fragments arranged by size: 

                1063     579     407
 
NgoMIV G'CCGG_C

Cuts at:      0    1148    1568    2049 
   Size:       1148     420     481

  Fragments arranged by size: 

                1148     481     420
 
NspI r_CATG'y

Cuts at:      0    1008    1161    1545    1845    2049 
   Size:       1008     153     384     300     204

  Fragments arranged by size: 

                1008     384     300     204     153
 
PasI CC'CwG_GG

Cuts at:      0     288    2049 
   Size:        288    1761
 
PciI A'CATG_T

Cuts at:      0    1004    2049 
   Size:       1004    1045
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     408    2049 
   Size:        408    1641
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1044    1076    2049 
   Size:       1044      32     973

  Fragments arranged by size: 

                1044     973      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1253    1475    1925    2049 
   Size:       1253     222     450     124

  Fragments arranged by size: 

                1253     450     222     124
 
PspOMI G'GGCC_C

Cuts at:      0    1204    2049 
   Size:       1204     845
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     184     216    2049 
   Size:        184      32    1833

  Fragments arranged by size: 

                1833     184      32
 
PstI C_TGCA'G

Cuts at:      0     544    2049 
   Size:        544    1505
 
PvuII CAG'CTG

Cuts at:      0     251     521    1303    1672    2049 
   Size:        251     270     782     369     377

  Fragments arranged by size: 

                 782     377     369     270     251
 
SacI G_AGCT'C

Cuts at:      0    1225    1609    1642    2049 
   Size:       1225     384      33     407

  Fragments arranged by size: 

                1225     407     384      33
 
SanDI GG'GwC_CC

Cuts at:      0    1475    2049 
   Size:       1475     574
 
ScaI AGT'ACT

Cuts at:      0    1459    2049 
   Size:       1459     590
 
SfcI C'TryA_G

Cuts at:      0     540    1143    1361    2049 
   Size:        540     603     218     688

  Fragments arranged by size: 

                 688     603     540     218
 
SmaI CCC'GGG

Cuts at:      0     796    1121    2049 
   Size:        796     325     928

  Fragments arranged by size: 

                 928     796     325
 
SphI G_CATG'C

Cuts at:      0    1545    1845    2049 
   Size:       1545     300     204

  Fragments arranged by size: 

                1545     300     204
 
SrfI GCCC'GGGC

Cuts at:      0     796    2049 
   Size:        796    1253
 
StyI C'CwwG_G

Cuts at:      0     407     938    1470    1910    2049 
   Size:        407     531     532     440     139

  Fragments arranged by size: 

                 532     531     440     407     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     656     704     885    1694    1806    2049 
   Size:        226     430      48     181     809     112     243

  Fragments arranged by size: 

                 809     430     243     226     181     112      48
 
TatI w'GTAC_w

Cuts at:      0     200    1409    1457    1850    2049 
   Size:        200    1209      48     393     199

  Fragments arranged by size: 

                1209     393     200     199      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29     344     376     493     525    2049 
   Size:         29     315      32     117      32    1524

  Fragments arranged by size: 

                1524     315     117      32      32      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     229     613    1561    2049 
   Size:        229     384     948     488

  Fragments arranged by size: 

                 948     488     384     229
 
XmaI C'CCGG_G

Cuts at:      0     794    1119    2049 
   Size:        794     325     930

  Fragments arranged by size: 

                 930     794     325
 
XmnI GAAnn'nnTTC

Cuts at:      0     340    2049 
   Size:        340    1709
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AloI     ApaI    ApaLI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI     BcgI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BpmI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI    CspCI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PpiI    PpuMI   PspOMI     PsrI
     PstI    PvuII     SacI    SanDI     ScaI     SfcI     SmaI     SphI
     SrfI     StyI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BlpI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsiWI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PvuI
    RsrII    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SspI     StuI     SwaI
  Tth111I     XbaI     XhoI     ZraI
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