################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspEI BspHI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Cfr10I CpoI DraI DraIII Eam1105I Eco47III EcoNI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 416 GACGTC GACGT^C AccI 1681 GTCTAC GT^[AC][GT]AC AflIII 968 ACATGT A^C[GA][CT]GT Asp700I 1123 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1715 TGGCCA TGG^CCA BciVI 960 GTATCC GTATCC(6/5) BglII 1498 AGATCT A^GATCT BlpI 467 GCTCAGC GC^T[ACGT]AGC Bpu1102I 467 GCTCAGC GC^T[ACGT]AGC BsaI 143 GAGACC GGTCTC(1/5) BseRI 997 GAGGAG GAGGAG(10/8) BsgI 7 GTGCAG GTGCAG(16/14) BsiEI 104 CGGCCG CG[GA][CT]^CG Bsp1407I 1357 TGTACA T^GTACA BspDI 874 ATCGAT AT^CGAT BspLU11I 968 ACATGT A^CATGT BsrGI 1357 TGTACA T^GTACA BstEII 1377 GGTAACC G^GT[ACGT]ACC BstPI 1377 GGTAACC G^GT[ACGT]ACC CelII 467 GCTCAGC GC^T[ACGT]AGC ClaI 874 ATCGAT AT^CGAT DrdI 325 GACGGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 104 CGGCCG C^GGCCG EclXI 104 CGGCCG C^GGCCG Eco52I 104 CGGCCG C^GGCCG EcoO65I 1377 GGTAACC G^GT[ACGT]ACC EcoRI 388 GAATTC G^AATTC HgaI 247 GCGTC GACGC(5/10) HincII 1507 GTTGAC GT[CT]^[GA]AC HindII 1507 GTTGAC GT[CT]^[GA]AC MluNI 1715 TGGCCA TGG^CCA MscI 1715 TGGCCA TGG^CCA NspI 968 ACATGT [GA]CATG^[CT] PflFI 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 59 AGGACCT [GA]G^G[AT]CC[CT] SacI 647 GAGCTC GAGCT^C ScaI 1542 AGTACT AGT^ACT SmaI 192 CCCGGG CCC^GGG SspBI 1357 TGTACA T^GTACA TfiI 1123 GAATC G^A[AT]TC Tth111I 1078 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 192 CCCGGG C^CCGGG XmaI 192 CCCGGG C^CCGGG XmnI 1123 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 388 GAATTC [GA]^AATT[CT] 1096 AAATTT AlwI 363 GATCC GGATC(4/5) 933 GGATC ApoI 388 GAATTC [GA]^AATT[CT] 1096 AAATTT AvaI 192 CCCGGG C^[CT]CG[GA]G 198 CTCGGG BbeI 115 GGCGCC GGCGC^C 342 GGCGCC BfaI 91 CTAG C^TAG 808 CTAG BglI 124 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 320 GCCCCGACGGC BsoBI 192 CCCGGG C^[CT]CG[GA]G 198 CTCGGG BspMI 1727 ACCTGC ACCTGC(4/8) 9 GCAGGT BsrBI 212 CCGCTC CCGCTC(-3/-3) 172 GAGCGG EarI 590 GAAGAG CTCTTC(1/4) 642 GAAGAG HaeII 115 GGCGCC [GA]GCGC^[CT] 342 GGCGCC KasI 115 GGCGCC G^GCGCC 342 GGCGCC Ksp632I 590 GAAGAG CTCTTC(1/4) 642 GAAGAG KspI 23 CCGCGG CCGC^GG 167 CCGCGG MaeI 91 CTAG C^TAG 808 CTAG NarI 115 GGCGCC GG^CGCC 342 GGCGCC NcoI 119 CCATGG C^CATGG 1235 CCATGG SacII 23 CCGCGG CCGC^GG 167 CCGCGG SfaNI 1741 GATGC GCATC(5/9) 1415 GCATC SfcI 462 CTACAG C^T[GA][CT]AG 1821 CTACAG SfoI 115 GGCGCC GGC^GCC 342 GGCGCC SmlI 650 CTCAAG C^T[CT][GA]AG 1502 CTCAAG TaqI 875 TCGA T^CGA 1305 TCGA TthHB8I 875 TCGA T^CGA 1305 TCGA XcmI 1382 CCAGAGAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1474 CCAGAACTGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AocI ApaI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII