Restriction map for pF1KSDA0821
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BciVI       BfrI        BlnI        BsaI        
BsaWI       BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eam1105I    EcoRV       EcoT22I     FspI        
HindIII     HpaI        KspI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SfuI        SgrAI       SnaBI       SspBI       
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       3535  GGTACC               G^GTACC
AcsI         3443  GAATTC               [GA]^AATT[CT]
AocI         4204  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       935  AGCGCT               AGC^GCT
ApoI         3443  GAATTC               [GA]^AATT[CT]
Asp718I      3535  GGTACC               G^GTACC
BalI         2624  TGGCCA               TGG^CCA
BcgI         1197  CGACCCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3848  TGATCA               T^GATCA
BglII        2546  AGATCT               A^GATCT
BlpI         2716  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2716  GCTGAGC              GC^T[ACGT]AGC
BsaBI        3846  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI        2159  GCAATG               GCAATG(2/0)
Bsu36I       4204  CCTGAGG              CC^T[ACGT]AGG
CelII        2716  GCTGAGC              GC^T[ACGT]AGC
DrdI          456  GACACACGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4094  CGGCCG               C^GGCCG
EclXI        4094  CGGCCG               C^GGCCG
Eco47III      935  AGCGCT               AGC^GCT
Eco52I       4094  CGGCCG               C^GGCCG
Eco81I       4204  CCTGAGG              CC^T[ACGT]AGG
EcoRI        3443  GAATTC               G^AATTC
FbaI         3848  TGATCA               T^GATCA
FseI         1469  GGCCGGCC             GGCCGG^CC
KpnI         3535  GGTACC               GGTAC^C
MamI         3846  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2624  TGGCCA               TGG^CCA
MscI         2624  TGGCCA               TGG^CCA
MseI         3137  TTAA                 T^TAA
NcoI         3539  CCATGG               C^CATGG
NruI         1128  TCGCGA               TCG^CGA
NspI         1487  GCATGC               [GA]CATG^[CT]
PaeR7I       1520  CTCGAG               C^TCGAG
PflMI         300  CCAGTGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         337  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1429  GAGCTC               GAGCT^C
SalI         2448  GTCGAC               G^TCGAC
SbfI         4308  CCTGCAGG             CCTGCA^GG
ScaI         2891  AGTACT               AGT^ACT
SfiI         2254  GGCCCGGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         2853  ACTAGT               A^CTAGT
SphI         1487  GCATGC               GCATG^C
Sse8387I     4308  CCTGCAGG             CCTGCA^GG
Tru9I        3137  TTAA                 T^TAA
Van91I        300  CCAGTGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1520  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

Asp700I       405  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2385  GAACCACTTC          
BamHI         536  GGATCC               G^GATCC
             3382  GGATCC              
BsiEI        2670  CGACCG               CG[GA][CT]^CG
             4094  CGGCCG              
DraIII        297  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1324  CACCCAGTG           
SapI         1431  GCTCTTC              GCTCTTC(1/4)
             2724  GCTCTTC             
XmnI          405  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2385  GAACCACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AatII       AccI        AccII       AciI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       AspHI       AspI        
AvaI        AvaII       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflFI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XcmI        XhoII       
XmaCI       XmaI        
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