Restriction map for pF1KSDA0821
 (Linear) (Six-base) MAPSORT of: KIAA0821.seq  Check: 674  from: 1  to: 4422



KIAA0821.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1920    3560    4422 
   Size:       1920    1640     862

  Fragments arranged by size: 

                1920    1640     862
 
AatII G_ACGT'C

Cuts at:      0     164    1709    1961    4422 
   Size:        164    1545     252    2461

  Fragments arranged by size: 

                2461    1545     252     164
 
AccI GT'mk_AC

Cuts at:      0     653    1545    2449    4422 
   Size:        653     892     904    1973

  Fragments arranged by size: 

                1973     904     892     653
 
Acc65I G'GTAC_C

Cuts at:      0    3535    4422 
   Size:       3535     887
 
AfeI AGC'GCT

Cuts at:      0     937    4422 
   Size:        937    3485
 
AflIII A'CryG_T

Cuts at:      0    1416    1418    2494    4422 
   Size:       1416       2    1076    1928

  Fragments arranged by size: 

                1928    1416    1076       2
 
AleI CACnn'nnGTG

Cuts at:      0     282    2593    4422 
   Size:        282    2311    1829

  Fragments arranged by size: 

                2311    1829     282
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3349    4422 
   Size:       3349    1073
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2071    2103    4422 
   Size:       2071      32    2319

  Fragments arranged by size: 

                2319    2071      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1592    1740    1770    2082    4422 
   Size:       1592     148      30     312    2340

  Fragments arranged by size: 

                2340    1592     312     148      30
 
ApaI G_GGCC'C

Cuts at:      0    1579    3796    3907    3974    4047    4214    4385    4422 
   Size:       1579    2217     111      67      73     167     171      37

  Fragments arranged by size: 

                2217    1579     171     167     111      73      67      37
            
 
ApaLI G'TGCA_C

Cuts at:      0    1933    2836    3310    3865    4422 
   Size:       1933     903     474     555     557

  Fragments arranged by size: 

                1933     903     557     555     474
 
ApoI r'AATT_y

Cuts at:      0    3443    4422 
   Size:       3443     979
 
AvaI C'yCGr_G

Cuts at:      0    1445    1520    1571    1682    2106    2256    3367    3781 
   Size:       1445      75      51     111     424     150    1111     414

Cuts at:   3781    3806    3973    4040    4422 
   Size:         25     167      67     382

  Fragments arranged by size: 

                1445    1111     424     414     382     167     150     111
                  75      67      51      25
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3519    3552    4422 
   Size:       3519      33     870

  Fragments arranged by size: 

                3519     870      33
 
BamHI G'GATC_C

Cuts at:      0     536    3382    4422 
   Size:        536    2846    1040

  Fragments arranged by size: 

                2846    1040     536
 
BanI G'GyrC_C

Cuts at:      0     146     661    1308    1333    1972    2272    2968    3535 
   Size:        146     515     647      25     639     300     696     567

Cuts at:   3535    3586    4105    4422 
   Size:         51     519     317

  Fragments arranged by size: 

                 696     647     639     567     519     515     317     300
                 146      51      25
 
BanII G_rGCy'C

Cuts at:      0      89     449    1433    1445    1562    1579    1690    3796 
   Size:         89     360     984      12     117      17     111    2106

Cuts at:   3796    3907    3923    3974    4047    4214    4385    4422 
   Size:        111      16      51      73     167     171      37

  Fragments arranged by size: 

                2106     984     360     171     167     117     111     111
                  89      73      51      37      17      16      12
 
BbeI G_GCGC'C

Cuts at:      0    1976    2276    4109    4422 
   Size:       1976     300    1833     313

  Fragments arranged by size: 

                1976    1833     313     300
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     402     660    2172    2328    3264    3642    4422 
   Size:        402     258    1512     156     936     378     780

  Fragments arranged by size: 

                1512     936     780     402     378     258     156
 
BbvCI CC'TCA_GC

Cuts at:      0    1231    1585    3396    4422 
   Size:       1231     354    1811    1026

  Fragments arranged by size: 

                1811    1231    1026     354
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1186    1220    4422 
   Size:       1186      34    3202

  Fragments arranged by size: 

                3202    1186      34
 
BclI T'GATC_A

Cuts at:      0    3848    4422 
   Size:       3848     574
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2261    2270    2831    3829    4162    4294    4346    4422 
   Size:       2261       9     561     998     333     132      52      76

  Fragments arranged by size: 

                2261     998     561     333     132      76      52       9
            
 
BglII A'GATC_T

Cuts at:      0    2546    4422 
   Size:       2546    1876
 
BlpI GC'TnA_GC

Cuts at:      0    2717    4422 
   Size:       2717    1705
 
Bme1580I G_kGCm'C

Cuts at:      0     151     421     913    1311    1579    1937    2840    3314 
   Size:        151     270     492     398     268     358     903     474

Cuts at:   3314    3376    3589    3796    3869    3907    3974    4047    4214 
   Size:         62     213     207      73      38      67      73     167

Cuts at:   4214    4385    4422 
   Size:        171      37

  Fragments arranged by size: 

                 903     492     474     398     358     270     268     213
                 207     171     167     151      73      73      67      62
                  38      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294    1196    1321    1372    4041    4208    4422 
   Size:        294     902     125      51    2669     167     214

  Fragments arranged by size: 

                2669     902     294     214     167     125      51
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     468     588    4422 
   Size:        468     120    3834

  Fragments arranged by size: 

                3834     468     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     463    1201    1349    1918    1984    2062    2395 
   Size:        385      78     738     148     569      66      78     333

Cuts at:   2395    2848    3033    3102    4039    4422 
   Size:        453     185      69     937     383

  Fragments arranged by size: 

                 937     738     569     453     385     383     333     185
                 148      78      78      69      66
 
Bpu10I CC'TnA_GC

Cuts at:      0      73    1231    1427    1585    3396    3797    3917    4422 
   Size:         73    1158     196     158    1811     401     120     505

  Fragments arranged by size: 

                1811    1158     505     401     196     158     120      73
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3091    3373    4390    4422 
   Size:       3091     282    1017      32

  Fragments arranged by size: 

                3091    1017     282      32
 
BsaAI yAC'GTr

Cuts at:      0     525     588     914     996    1419    2495    4422 
   Size:        525      63     326      82     423    1076    1927

  Fragments arranged by size: 

                1927    1076     525     423     326      82      63
 
BsaBI GATnn'nnATC

Cuts at:      0    3850    4422 
   Size:       3850     572
 
BsaHI Gr'CG_yC

Cuts at:      0     161     260    1706    1958    1973    2273    4106    4332 
   Size:        161      99    1446     252      15     300    1833     226

Cuts at:   4332    4422 
   Size:         90

  Fragments arranged by size: 

                1833    1446     300     252     226     161      99      90
                  15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133     358     388    1582    1612    1696    1726 
   Size:        103      30     225      30    1194      30      84      30

Cuts at:   1726    2071    2101    3739    3769    4168    4198    4422 
   Size:        345      30    1638      30     399      30     224

  Fragments arranged by size: 

                1638    1194     399     345     225     224     103      84
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1078    1098    1701    1704    1942    1981    2113    3193 
   Size:       1078      20     603       3     238      39     132    1080

Cuts at:   3193    3627    4036    4116    4422 
   Size:        434     409      80     306

  Fragments arranged by size: 

                1080    1078     603     434     409     306     238     132
                  80      39      20       3
 
BseYI C'CCAG_C

Cuts at:      0     782    1212    1347    1384    1401    1478    1757    2436 
   Size:        782     430     135      37      17      77     279     679

Cuts at:   2436    3150    3177    4422 
   Size:        714      27    1245

  Fragments arranged by size: 

                1245     782     714     679     430     279     135      77
                  37      27      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     391    1578    2095    2773    3061    4074    4422 
   Size:        262     129    1187     517     678     288    1013     348

  Fragments arranged by size: 

                1187    1013     678     517     348     288     262     129
            
 
BsiEI CG_ry'CG

Cuts at:      0    2673    4097    4422 
   Size:       2673    1424     325

  Fragments arranged by size: 

                2673    1424     325
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     473    1433    1937    2531    2840    3107    3314 
   Size:         23     450     960     504     594     309     267     207

Cuts at:   3314    3869    4422 
   Size:        555     553

  Fragments arranged by size: 

                 960     594     555     553     504     450     309     267
                 207      23
 
BsmI GAATG_Cn'

Cuts at:      0     183    1923    2229    4422 
   Size:        183    1740     306    2193

  Fragments arranged by size: 

                2193    1740     306     183
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     730     913    1713    4422 
   Size:        730     183     800    2709

  Fragments arranged by size: 

                2709     800     730     183
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      89     151     421     449     473     913    1311 
   Size:         23      66      62     270      28      24     440     398

Cuts at:   1311    1433    1445    1562    1579    1690    1937    2531    2840 
   Size:        122      12     117      17     111     247     594     309

Cuts at:   2840    3107    3314    3376    3589    3796    3869    3907    3923 
   Size:        267     207      62     213     207      73      38      16

Cuts at:   3923    3974    4047    4214    4385    4422 
   Size:         51      73     167     171      37

  Fragments arranged by size: 

                 594     440     398     309     270     267     247     213
                 207     207     171     167     122     117     111      73
                  73      66      62      62      51      38      37      28
                  24      23      17      16      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     260    1920    2855    3560    3884    4178    4422 
   Size:        260    1660     935     705     324     294     244

  Fragments arranged by size: 

                1660     935     705     324     294     260     244
 
BsrBI CCG'CTC

Cuts at:      0     731    1299    3359    4422 
   Size:        731     568    2060    1063

  Fragments arranged by size: 

                2060    1063     731     568
 
BsrDI GCAATG_nn'

Cuts at:      0    2166    4422 
   Size:       2166    2256
 
BsrFI r'CCGG_y

Cuts at:      0     316    1470    1795    2242    2779    4115    4422 
   Size:        316    1154     325     447     537    1336     307

  Fragments arranged by size: 

                1336    1154     537     447     325     316     307
 
BssSI C'ACGA_G

Cuts at:      0     460     573    4342    4422 
   Size:        460     113    3769      80

  Fragments arranged by size: 

                3769     460     113      80
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     302     438    1488    1770    4422 
   Size:        302     136    1050     282    2652

  Fragments arranged by size: 

                2652    1050     302     282     136
 
BstEII G'GTnAC_C

Cuts at:      0      51     511    3069    4410    4422 
   Size:         51     460    2558    1341      12

  Fragments arranged by size: 

                2558    1341     460      51      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     630    1668    3126    4422 
   Size:        630    1038    1458    1296

  Fragments arranged by size: 

                1458    1296    1038     630
 
BstYI r'GATC_y

Cuts at:      0     536    2546    3382    4422 
   Size:        536    2010     836    1040

  Fragments arranged by size: 

                2010    1040     836     536
 
Bsu36I CC'TnA_GG

Cuts at:      0    4205    4422 
   Size:       4205     217
 
BtgI C'CryG_G

Cuts at:      0    1856    1922    2216    2363    3539    3579    4422 
   Size:       1856      66     294     147    1176      40     843

  Fragments arranged by size: 

                1856    1176     843     294     147      66      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     227    1641    2286    2922    3144    3594    4422 
   Size:        227    1414     645     636     222     450     828

  Fragments arranged by size: 

                1414     828     645     636     450     227     222
 
BtsI GCAGTG_nn'

Cuts at:      0      23     250     895    3288    4422 
   Size:         23     227     645    2393    1134

  Fragments arranged by size: 

                2393    1134     645     227      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     302    1329    4422 
   Size:        302    1027    3093

  Fragments arranged by size: 

                3093    1027     302
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     462    4422 
   Size:        462    3960
 
EaeI y'GGCC_r

Cuts at:      0      59     590    1468    1472    1649    1885    1940    1985 
   Size:         59     531     878       4     177     236      55      45

Cuts at:   1985    2240    2624    3896    4094    4137    4422 
   Size:        255     384    1272     198      43     285

  Fragments arranged by size: 

                1272     878     531     384     285     255     236     198
                 177      59      55      45      43       4
 
EagI C'GGCC_G

Cuts at:      0    4094    4422 
   Size:       4094     328
 
EarI CTCTTCn'nnn_

Cuts at:      0    1438    2212    2713    2731    3211    3247    3953    4422 
   Size:       1438     774     501      18     480      36     706     469

  Fragments arranged by size: 

                1438     774     706     501     480     469      36      18
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     803    1291    4422 
   Size:        803     488    3131

  Fragments arranged by size: 

                3131     803     488
 
EcoICRI GAG'CTC

Cuts at:      0    1431    4422 
   Size:       1431    2991
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     463    1201    1349    1918    1984    2062    2395 
   Size:        385      78     738     148     569      66      78     333

Cuts at:   2395    2848    3033    3102    4039    4422 
   Size:        453     185      69     937     383

  Fragments arranged by size: 

                 937     738     569     453     385     383     333     185
                 148      78      78      69      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     433    1966    3938    4422 
   Size:        433    1533    1972     484

  Fragments arranged by size: 

                1972    1533     484     433
 
EcoO109I rG'GnC_Cy

Cuts at:      0     350     425     495     969    1351    1502    1575    1576 
   Size:        350      75      70     474     382     151      73       1

Cuts at:   1576    1767    3431    3792    3793    3903    3904    4014    4210 
   Size:        191    1664     361       1     110       1     110     196

Cuts at:   4210    4221    4381    4422 
   Size:         11     160      41

  Fragments arranged by size: 

                1664     474     382     361     350     196     191     160
                 151     110     110      75      73      70      41      11
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    3443    4422 
   Size:       3443     979
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2406    3705    4422 
   Size:       2406    1299     717

  Fragments arranged by size: 

                2406    1299     717
 
FseI GG_CCGG'CC

Cuts at:      0    1474    4422 
   Size:       1474    2948
 
HaeII r_GCGC'y

Cuts at:      0     939    1976    2276    3151    4109    4422 
   Size:        939    1037     300     875     958     313

  Fragments arranged by size: 

                1037     958     939     875     313     300
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     460     492     922     954    1696    1728    1774    1806 
   Size:        460      32     430      32     742      32      46      32

Cuts at:   1806    4168    4200    4422 
   Size:       2362      32     222

  Fragments arranged by size: 

                2362     742     460     430     222      46      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1116    1611    2450    3051    4422 
   Size:       1116     495     839     601    1371

  Fragments arranged by size: 

                1371    1116     839     601     495
 
KasI G'GCGC_C

Cuts at:      0    1972    2272    4105    4422 
   Size:       1972     300    1833     317

  Fragments arranged by size: 

                1972    1833     317     300
 
KpnI G_GTAC'C

Cuts at:      0    3539    4422 
   Size:       3539     883
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     971    2501    4061    4422 
   Size:        971    1530    1560     361

  Fragments arranged by size: 

                1560    1530     971     361
 
MscI TGG'CCA

Cuts at:      0    2626    4422 
   Size:       2626    1796
 
MslI CAynn'nnrTG

Cuts at:      0     169     282     523    1492    2393    2593    4422 
   Size:        169     113     241     969     901     200    1829

  Fragments arranged by size: 

                1829     969     901     241     200     169     113
 
MspA1I CmG'CkG

Cuts at:      0     501     782     882    1140    1347    1737    1761    2127 
   Size:        501     281     100     258     207     390      24     366

Cuts at:   2127    4160    4407    4422 
   Size:       2033     247      15

  Fragments arranged by size: 

                2033     501     390     366     281     258     247     207
                 100      24      15
 
NaeI GCC'GGC

Cuts at:      0     318    1472    1797    2244    2781    4117    4422 
   Size:        318    1154     325     447     537    1336     305

  Fragments arranged by size: 

                1336    1154     537     447     325     318     305
 
NarI GG'CG_CC

Cuts at:      0    1973    2273    4106    4422 
   Size:       1973     300    1833     316

  Fragments arranged by size: 

                1973    1833     316     300
 
NcoI C'CATG_G

Cuts at:      0    3539    4422 
   Size:       3539     883
 
NgoMIV G'CCGG_C

Cuts at:      0     316    1470    1795    2242    2779    4115    4422 
   Size:        316    1154     325     447     537    1336     307

  Fragments arranged by size: 

                1336    1154     537     447     325     316     307
 
NruI TCG'CGA

Cuts at:      0    1130    4422 
   Size:       1130    3292
 
NspI r_CATG'y

Cuts at:      0    1491    4422 
   Size:       1491    2931
 
PasI CC'CwG_GG

Cuts at:      0     421    1604    2266    3661    4422 
   Size:        421    1183     662    1395     761

  Fragments arranged by size: 

                1395    1183     761     662     421
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306    4422 
   Size:        306    4116
 
PfoI T'CCnGG_A

Cuts at:      0    1748    1903    1961    2039    4422 
   Size:       1748     155      58      78    2383

  Fragments arranged by size: 

                2383    1748     155      78      58
 
PmlI CAC'GTG

Cuts at:      0     914    1419    2495    4422 
   Size:        914     505    1076    1927

  Fragments arranged by size: 

                1927    1076     914     505
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2071    2103    4422 
   Size:       2071      32    2319

  Fragments arranged by size: 

                2319    2071      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     350     425     495     969    1351    3431    4422 
   Size:        350      75      70     474     382    2080     991

  Fragments arranged by size: 

                2080     991     474     382     350      75      70
 
PshAI GACnn'nnGTC

Cuts at:      0     341    4422 
   Size:        341    4081
 
PspOMI G'GGCC_C

Cuts at:      0    1575    3792    3903    3970    4043    4210    4381    4422 
   Size:       1575    2217     111      67      73     167     171      41

  Fragments arranged by size: 

                2217    1575     171     167     111      73      67      41
            
 
PspXI vC'TCGA_Gb

Cuts at:      0    1520    4422 
   Size:       1520    2902
 
PstI C_TGCA'G

Cuts at:      0     434     506    1766    2506    2666    3575    4313    4422 
   Size:        434      72    1260     740     160     909     738     109

  Fragments arranged by size: 

                1260     909     740     738     434     160     109      72
            
 
PvuII CAG'CTG

Cuts at:      0     882    1140    1347    1737    1761    2127    4407    4422 
   Size:        882     258     207     390      24     366    2280      15

  Fragments arranged by size: 

                2280     882     390     366     258     207      24      15
            
 
SacI G_AGCT'C

Cuts at:      0    1433    4422 
   Size:       1433    2989
 
SalI G'TCGA_C

Cuts at:      0    2448    4422 
   Size:       2448    1974
 
SanDI GG'GwC_CC

Cuts at:      0     425    3431    4422 
   Size:        425    3006     991

  Fragments arranged by size: 

                3006     991     425
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1438    2731    4422 
   Size:       1438    1293    1691

  Fragments arranged by size: 

                1691    1438    1293
 
SbfI CC_TGCA'GG

Cuts at:      0    4313    4422 
   Size:       4313     109
 
ScaI AGT'ACT

Cuts at:      0    2893    4422 
   Size:       2893    1529
 
SexAI A'CCwGG_T

Cuts at:      0    1613    3053    4322    4422 
   Size:       1613    1440    1269     100

  Fragments arranged by size: 

                1613    1440    1269     100
 
SfcI C'TryA_G

Cuts at:      0     430     502    1173    1762    2502    2662    3571    4309 
   Size:        430      72     671     589     740     160     909     738

Cuts at:   4309    4422 
   Size:        113

  Fragments arranged by size: 

                 909     740     738     671     589     430     160     113
                  72
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2261    4422 
   Size:       2261    2161
 
SfoI GGC'GCC

Cuts at:      0    1974    2274    4107    4422 
   Size:       1974     300    1833     315

  Fragments arranged by size: 

                1974    1833     315     300
 
SmaI CCC'GGG

Cuts at:      0    1573    1684    2258    3369    3783    3975    4042    4422 
   Size:       1573     111     574    1111     414     192      67     380

  Fragments arranged by size: 

                1573    1111     574     414     380     192     111      67
            
 
SmlI C'TyrA_G

Cuts at:      0    1520    3106    3388    4369    4422 
   Size:       1520    1586     282     981      53

  Fragments arranged by size: 

                1586    1520     981     282      53
 
SpeI A'CTAG_T

Cuts at:      0    2853    4422 
   Size:       2853    1569
 
SphI G_CATG'C

Cuts at:      0    1491    4422 
   Size:       1491    2931
 
StuI AGG'CCT

Cuts at:      0      71    2980    4367    4422 
   Size:         71    2909    1387      55

  Fragments arranged by size: 

                2909    1387      71      55
 
StyI C'CwwG_G

Cuts at:      0      66    1511    1553    2024    2454    3539    3689    3899 
   Size:         66    1445      42     471     430    1085     150     210

Cuts at:   3899    4422 
   Size:        523

  Fragments arranged by size: 

                1445    1085     523     471     430     210     150      66
                  42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     313     811    1321    1340    1492    2571    2583 
   Size:         79     234     498     510      19     152    1079      12

Cuts at:   2583    2683    2893    4422 
   Size:        100     210    1529

  Fragments arranged by size: 

                1529    1079     510     498     234     210     152     100
                  79      19      12
 
TatI w'GTAC_w

Cuts at:      0    2891    3320    4422 
   Size:       2891     429    1102

  Fragments arranged by size: 

                2891    1102     429
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      18      50     103     135    4422 
   Size:         18      32      53      32    4287

  Fragments arranged by size: 

                4287      53      32      32      18
 
Tth111I GACn'n_nGTC

Cuts at:      0     382    1993    2350    3483    4422 
   Size:        382    1611     357    1133     939

  Fragments arranged by size: 

                1611    1133     939     382     357
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     954    1326    1344    2031    2235    3687    4422 
   Size:        954     372      18     687     204    1452     735

  Fragments arranged by size: 

                1452     954     735     687     372     204      18
 
XhoI C'TCGA_G

Cuts at:      0    1520    4422 
   Size:       1520    2902
 
XmaI C'CCGG_G

Cuts at:      0    1571    1682    2256    3367    3781    3973    4040    4422 
   Size:       1571     111     574    1111     414     192      67     382

  Fragments arranged by size: 

                1571    1111     574     414     382     192     111      67
            
 
XmnI GAAnn'nnTTC

Cuts at:      0     409    2389    4422 
   Size:        409    1980    2033

  Fragments arranged by size: 

                2033    1980     409
 
ZraI GAC'GTC

Cuts at:      0     162    1707    1959    4422 
   Size:        162    1545     252    2463

  Fragments arranged by size: 

                2463    1545     252     162
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NruI     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI     AcuI    AflII     AgeI     AhdI     AscI     AseI    AsiSI
    AvrII    BciVI    BfrBI    BmgBI     BmtI     BsaI    BsaWI    BsiWI
    BspEI    BspHI    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI
     DraI    EcoRV     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NsiI     PacI     PciI     PmeI     PsiI
     PsrI     PvuI    RsrII    SacII     SgfI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XbaI
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