Restriction map for pF1KSDA0813
 (Linear) (Six-base) MAPSORT of: KIAA0813.seq  Check: 227  from: 1  to: 4602



KIAA0813.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     124     829     868    2261    2824    2941    3091    3217 
   Size:        124     705      39    1393     563     117     150     126

Cuts at:   3217    4602 
   Size:       1385

  Fragments arranged by size: 

                1393    1385     705     563     150     126     124     117
                  39
 
AccI GT'mk_AC

Cuts at:      0    2341    4602 
   Size:       2341    2261
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1606    1720    2133    4528    4602 
   Size:       1606     114     413    2395      74

  Fragments arranged by size: 

                2395    1606     413     114      74
 
AflII C'TTAA_G

Cuts at:      0    2394    4602 
   Size:       2394    2208
 
AflIII A'CryG_T

Cuts at:      0     365    4602 
   Size:        365    4237
 
AleI CACnn'nnGTG

Cuts at:      0    2208    4154    4602 
   Size:       2208    1946     448

  Fragments arranged by size: 

                2208    1946     448
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     169    1805    4278    4288    4602 
   Size:        169    1636    2473      10     314

  Fragments arranged by size: 

                2473    1636     314     169      10
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     367     368     399     400    2947    2979    4602 
   Size:        367       1      31       1    2547      32    1623

  Fragments arranged by size: 

                2547    1623     367      32      31       1       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     687    1155    1209    1299    1768    4281    4602 
   Size:        687     468      54      90     469    2513     321

  Fragments arranged by size: 

                2513     687     469     468     321      90      54
 
ApaI G_GGCC'C

Cuts at:      0     733    2262    2687    3200    3288    4079    4251    4602 
   Size:        733    1529     425     513      88     791     172     351

  Fragments arranged by size: 

                1529     791     733     513     425     351     172      88
            
 
ApaLI G'TGCA_C

Cuts at:      0     855    4602 
   Size:        855    3747
 
ApoI r'AATT_y

Cuts at:      0     480    4602 
   Size:        480    4122
 
AvaI C'yCGr_G

Cuts at:      0      13     726     922    4221    4602 
   Size:         13     713     196    3299     381

  Fragments arranged by size: 

                3299     713     381     196      13
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1937    1970    4602 
   Size:       1937      33    2632

  Fragments arranged by size: 

                2632    1937      33
 
BamHI G'GATC_C

Cuts at:      0     224    3602    4602 
   Size:        224    3378    1000

  Fragments arranged by size: 

                3378    1000     224
 
BanI G'GyrC_C

Cuts at:      0      80      88    1387    1409    2590    2812    2929    3184 
   Size:         80       8    1299      22    1181     222     117     255

Cuts at:   3184    3846    3919    4005    4306    4602 
   Size:        662      73      86     301     296

  Fragments arranged by size: 

                1299    1181     662     301     296     255     222     117
                  86      80      73      22       8
 
BanII G_rGCy'C

Cuts at:      0      53     251     713     733    2262    2687    3200    3288 
   Size:         53     198     462      20    1529     425     513      88

Cuts at:   3288    3388    3799    4079    4251    4262    4471    4602 
   Size:        100     411     280     172      11     209     131

  Fragments arranged by size: 

                1529     513     462     425     411     280     209     198
                 172     131     100      88      53      20      11
 
BbeI G_GCGC'C

Cuts at:      0      84    1413    3850    4602 
   Size:         84    1329    2437     752

  Fragments arranged by size: 

                2437    1329     752      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2650    4602 
   Size:       2650    1952
 
BbvCI CC'TCA_GC

Cuts at:      0     688    2443    3329    4602 
   Size:        688    1755     886    1273

  Fragments arranged by size: 

                1755    1273     886     688
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     855     889    3616    3650    4602 
   Size:        855      34    2727      34     952

  Fragments arranged by size: 

                2727     952     855      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1965    4602 
   Size:       1965    2637
 
BglI GCCn_nnn'nGGC

Cuts at:      0      42     436     746     910    2861    3464    4046    4286 
   Size:         42     394     310     164    1951     603     582     240

Cuts at:   4286    4602 
   Size:        316

  Fragments arranged by size: 

                1951     603     582     394     316     310     240     164
                  42
 
BlpI GC'TnA_GC

Cuts at:      0    1461    4602 
   Size:       1461    3141
 
Bme1580I G_kGCm'C

Cuts at:      0     103     733     818     859    1270    1392    2203    2262 
   Size:        103     630      85      41     411     122     811      59

Cuts at:   2262    2687    2815    2932    3094    3187    3200    3288    4079 
   Size:        425     128     117     162      93      13      88     791

Cuts at:   4079    4178    4230    4251    4320    4477    4593    4602 
   Size:         99      52      21      69     157     116       9

  Fragments arranged by size: 

                 811     791     630     425     411     162     157     128
                 122     117     116     103      99      93      88      85
                  69      59      52      41      21      13       9
 
BmgBI CAC'GTC

Cuts at:      0    1530    2133    2562    4602 
   Size:       1530     603     429    2040

  Fragments arranged by size: 

                2040    1530     603     429
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     714    1611    1682    3168    3282    4602 
   Size:        714     897      71    1486     114    1320

  Fragments arranged by size: 

                1486    1320     897     714     114      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     568     967     998    1563    1684    1822    1963 
   Size:        352     216     399      31     565     121     138     141

Cuts at:   1963    4602 
   Size:       2639

  Fragments arranged by size: 

                2639     565     399     352     216     141     138     121
                  31
 
Bpu10I CC'TnA_GC

Cuts at:      0     688     789    1018    1604    2371    2443    3329    4602 
   Size:        688     101     229     586     767      72     886    1273

  Fragments arranged by size: 

                1273     886     767     688     586     229     101      72
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     438    1324    1863    2854    4602 
   Size:        235     203     886     539     991    1748

  Fragments arranged by size: 

                1748     991     886     539     235     203
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     919    3585    4602 
   Size:        919    2666    1017

  Fragments arranged by size: 

                2666    1017     919
 
BsaAI yAC'GTr

Cuts at:      0    2969    4602 
   Size:       2969    1633
 
BsaBI GATnn'nnATC

Cuts at:      0    1657    4602 
   Size:       1657    2945
 
BsaHI Gr'CG_yC

Cuts at:      0      81    1052    1410    3053    3847    4602 
   Size:         81     971     358    1643     794     755

  Fragments arranged by size: 

                1643     971     794     755     358      81
 
BsaWI w'CCGG_w

Cuts at:      0     121     372    1454    1811    3144    4602 
   Size:        121     251    1082     357    1333    1458

  Fragments arranged by size: 

                1458    1333    1082     357     251     121
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1985    2015    2551    2581    3298    3328    4602 
   Size:       1985      30     536      30     717      30    1274

  Fragments arranged by size: 

                1985    1274     717     536      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1912    2185    2701    4573    4602 
   Size:       1912     273     516    1872      29

  Fragments arranged by size: 

                1912    1872     516     273      29
 
BseYI C'CCAG_C

Cuts at:      0     732    2636    2680    2857    3691    4100    4602 
   Size:        732    1904      44     177     834     409     502

  Fragments arranged by size: 

                1904     834     732     502     409     177      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     102     346     619     807    1285    1765    4096 
   Size:         96       6     244     273     188     478     480    2331

Cuts at:   4096    4554    4602 
   Size:        458      48

  Fragments arranged by size: 

                2331     480     478     458     273     244     188      96
                  48       6
 
BsiEI CG_ry'CG

Cuts at:      0      46    4602 
   Size:         46    4556
 
BsiHKAI G_wGCw'C

Cuts at:      0      53     537     727     859    1462    1579    2974    3142 
   Size:         53     484     190     132     603     117    1395     168

Cuts at:   3142    4602 
   Size:       1460

  Fragments arranged by size: 

                1460    1395     603     484     190     168     132     117
                  53
 
BsmI GAATG_Cn'

Cuts at:      0    1170    2273    2743    4602 
   Size:       1170    1103     470    1859

  Fragments arranged by size: 

                1859    1170    1103     470
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1372    3709    4602 
   Size:       1372    2337     893

  Fragments arranged by size: 

                2337    1372     893
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     103     251     537     713     727     733     818 
   Size:         53      50     148     286     176      14       6      85

Cuts at:    818     859    1270    1392    1462    1579    2203    2262    2687 
   Size:         41     411     122      70     117     624      59     425

Cuts at:   2687    2815    2932    2974    3094    3142    3187    3200    3288 
   Size:        128     117      42     120      48      45      13      88

Cuts at:   3288    3388    3799    4079    4178    4230    4251    4262    4320 
   Size:        100     411     280      99      52      21      11      58

Cuts at:   4320    4471    4477    4593    4602 
   Size:        151       6     116       9

  Fragments arranged by size: 

                 624     425     411     411     286     280     176     151
                 148     128     122     120     117     117     116     100
                  99      88      85      70      59      58      53      52
                  50      48      45      42      41      21      14      13
                  11       9       6       6
 
BspEI T'CCGG_A

Cuts at:      0    1811    3144    4602 
   Size:       1811    1333    1458

  Fragments arranged by size: 

                1811    1458    1333
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     124     829     868     926    1217    2261    2363    2824 
   Size:        124     705      39      58     291    1044     102     461

Cuts at:   2824    2941    3091    3217    3536    3551    3567    4602 
   Size:        117     150     126     319      15      16    1035

  Fragments arranged by size: 

                1044    1035     705     461     319     291     150     126
                 124     117     102      58      39      16      15
 
BsrBI CCG'CTC

Cuts at:      0     336    1070    1817    2495    4602 
   Size:        336     734     747     678    2107

  Fragments arranged by size: 

                2107     747     734     678     336
 
BsrDI GCAATG_nn'

Cuts at:      0     873    2529    2666    3632    4174    4602 
   Size:        873    1656     137     966     542     428

  Fragments arranged by size: 

                1656     966     873     542     428     137
 
BsrFI r'CCGG_y

Cuts at:      0      36    2983    3922    4602 
   Size:         36    2947     939     680

  Fragments arranged by size: 

                2947     939     680      36
 
BsrGI T'GTAC_A

Cuts at:      0    2840    3611    3950    4602 
   Size:       2840     771     339     652

  Fragments arranged by size: 

                2840     771     652     339
 
BssSI C'ACGA_G

Cuts at:      0    2362    3166    4470    4602 
   Size:       2362     804    1304     132

  Fragments arranged by size: 

                2362    1304     804     132
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1877    4173    4281    4602 
   Size:       1877    2296     108     321

  Fragments arranged by size: 

                2296    1877     321     108
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1569    1800    3426    3807    4602 
   Size:       1569     231    1626     381     795

  Fragments arranged by size: 

                1626    1569     795     381     231
 
BstYI r'GATC_y

Cuts at:      0     224    1247    1598    3147    3356    3602    3908    4602 
   Size:        224    1023     351    1549     209     246     306     694

  Fragments arranged by size: 

                1549    1023     694     351     306     246     224     209
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    4302    4602 
   Size:       4302     300
 
BtgI C'CryG_G

Cuts at:      0     128     141    1119    1622    2228    2517    3556    3584 
   Size:        128      13     978     503     606     289    1039      28

Cuts at:   3584    3806    3872    3930    4143    4161    4602 
   Size:        222      66      58     213      18     441

  Fragments arranged by size: 

                1039     978     606     503     441     289     222     213
                 128      66      58      28      18      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2103    4553    4602 
   Size:       2103    2450      49

  Fragments arranged by size: 

                2450    2103      49
 
BtsI GCAGTG_nn'

Cuts at:      0     688     899    1222    2467    3246    4086    4282    4290 
   Size:        688     211     323    1245     779     840     196       8

Cuts at:   4290    4602 
   Size:        312

  Fragments arranged by size: 

                1245     840     779     688     323     312     211     196
                   8
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     634     669    2970    3005    4602 
   Size:        634      35    2301      35    1597

  Fragments arranged by size: 

                2301    1597     634      35      35
 
DraI TTT'AAA

Cuts at:      0    1683    4602 
   Size:       1683    2919
 
DraIII CAC_nnn'GTG

Cuts at:      0     722    4324    4326    4580    4602 
   Size:        722    3602       2     254      22

  Fragments arranged by size: 

                3602     722     254      22       2
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2230    2571    4602 
   Size:       2230     341    2031

  Fragments arranged by size: 

                2230    2031     341
 
EaeI y'GGCC_r

Cuts at:      0      43     697     848     911    2006    2582    3553    4146 
   Size:         43     654     151      63    1095     576     971     593

Cuts at:   4146    4287    4602 
   Size:        141     315

  Fragments arranged by size: 

                1095     971     654     593     576     315     151     141
                  63      43
 
EagI C'GGCC_G

Cuts at:      0      43    4602 
   Size:         43    4559
 
EarI CTCTTCn'nnn_

Cuts at:      0     718     766    4602 
   Size:        718      48    3836

  Fragments arranged by size: 

                3836     718      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     619    3886    4602 
   Size:        619    3267     716

  Fragments arranged by size: 

                3267     716     619
 
EcoICRI GAG'CTC

Cuts at:      0      51    4602 
   Size:         51    4551
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     568     967     998    1563    1606    1684    1720 
   Size:        352     216     399      31     565      43      78      36

Cuts at:   1720    1822    1963    2133    4528    4602 
   Size:        102     141     170    2395      74

  Fragments arranged by size: 

                2395     565     399     352     216     170     141     102
                  78      74      43      36      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1261    2266    2542    4602 
   Size:       1261    1005     276    2060

  Fragments arranged by size: 

                2060    1261    1005     276
 
EcoO109I rG'GnC_Cy

Cuts at:      0      23     524    1062    1206    1748    1847    2259    2684 
   Size:         23     501     538     144     542      99     412     425

Cuts at:   2684    2746    3284    4075    4247    4248    4383    4497    4544 
   Size:         62     538     791     172       1     135     114      47

Cuts at:   4544    4602 
   Size:         58

  Fragments arranged by size: 

                 791     542     538     538     501     425     412     172
                 144     135     114      99      62      58      47      23
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     255    4542    4602 
   Size:        255    4287      60

  Fragments arranged by size: 

                4287     255      60
 
FspI TGC'GCA

Cuts at:      0    1363    1909    4599    4602 
   Size:       1363     546    2690       3

  Fragments arranged by size: 

                2690    1363     546       3
 
HaeII r_GCGC'y

Cuts at:      0       7      84     195    1413    3850    4602 
   Size:          7      77     111    1218    2437     752

  Fragments arranged by size: 

                2437    1218     752     111      77       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1011    1043    2551    2583    3124    3156    3759    3791 
   Size:       1011      32    1508      32     541      32     603      32

Cuts at:   3791    3868    3900    4204    4236    4351    4383    4531    4563 
   Size:         77      32     304      32     115      32     148      32

Cuts at:   4563    4602 
   Size:         39

  Fragments arranged by size: 

                1508    1011     603     541     304     148     115      77
                  39      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3163    3306    3504    3882    4602 
   Size:       3163     143     198     378     720

  Fragments arranged by size: 

                3163     720     378     198     143
 
HindIII A'AGCT_T

Cuts at:      0     452    4602 
   Size:        452    4150
 
KasI G'GCGC_C

Cuts at:      0      80    1409    3846    4602 
   Size:         80    1329    2437     756

  Fragments arranged by size: 

                2437    1329     756      80
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    2776    4602 
   Size:        665    2111    1826

  Fragments arranged by size: 

                2111    1826     665
 
MscI TGG'CCA

Cuts at:      0    2008    2584    3555    4148    4289    4602 
   Size:       2008     576     971     593     141     313

  Fragments arranged by size: 

                2008     971     593     576     313     141
 
MslI CAynn'nnrTG

Cuts at:      0     233     589    1675    2208    2416    2522    3038    3662 
   Size:        233     356    1086     533     208     106     516     624

Cuts at:   3662    3805    4154    4602 
   Size:        143     349     448

  Fragments arranged by size: 

                1086     624     533     516     448     356     349     233
                 208     143     106
 
MspA1I CmG'CkG

Cuts at:      0     258     273     360     489     509     696     785    1394 
   Size:        258      15      87     129      20     187      89     609

Cuts at:   1394    1506    1553    1800    1877    2034    2337    2427    2624 
   Size:        112      47     247      77     157     303      90     197

Cuts at:   2624    2636    2861    2900    3126    3272    3889    4129    4199 
   Size:         12     225      39     226     146     617     240      70

Cuts at:   4199    4602 
   Size:        403

  Fragments arranged by size: 

                 617     609     403     303     258     247     240     226
                 225     197     187     157     146     129     112      90
                  89      87      77      70      47      39      20      15
                  12
 
NarI GG'CG_CC

Cuts at:      0      81    1410    3847    4602 
   Size:         81    1329    2437     755

  Fragments arranged by size: 

                2437    1329     755      81
 
NcoI C'CATG_G

Cuts at:      0    3806    4143    4161    4602 
   Size:       3806     337      18     441

  Fragments arranged by size: 

                3806     441     337      18
 
NdeI CA'TA_TG

Cuts at:      0     608    4602 
   Size:        608    3994
 
NspI r_CATG'y

Cuts at:      0     369    1824    3216    4602 
   Size:        369    1455    1392    1386

  Fragments arranged by size: 

                1455    1392    1386     369
 
PasI CC'CwG_GG

Cuts at:      0    4072    4602 
   Size:       4072     530
 
PciI A'CATG_T

Cuts at:      0     365    4602 
   Size:        365    4237
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2694    3004    3180    3294    4602 
   Size:       2694     310     176     114    1308

  Fragments arranged by size: 

                2694    1308     310     176     114
 
PfoI T'CCnGG_A

Cuts at:      0     975    1202    1661    2328    4391    4602 
   Size:        975     227     459     667    2063     211

  Fragments arranged by size: 

                2063     975     667     459     227     211
 
PmlI CAC'GTG

Cuts at:      0    2969    4602 
   Size:       2969    1633
 
PpuMI rG'GwC_Cy

Cuts at:      0      23    1206    1847    2746    4383    4544    4602 
   Size:         23    1183     641     899    1637     161      58

  Fragments arranged by size: 

                1637    1183     899     641     161      58      23
 
PshAI GACnn'nnGTC

Cuts at:      0    2337    4602 
   Size:       2337    2265
 
PspOMI G'GGCC_C

Cuts at:      0     729    2258    2683    3196    3284    4075    4247    4602 
   Size:        729    1529     425     513      88     791     172     355

  Fragments arranged by size: 

                1529     791     729     513     425     355     172      88
            
 
PstI C_TGCA'G

Cuts at:      0     158     272     865    1214    2129    2360    2462    3101 
   Size:        158     114     593     349     915     231     102     639

Cuts at:   3101    3241    3262    3533    3971    4267    4277    4602 
   Size:        140      21     271     438     296      10     325

  Fragments arranged by size: 

                 915     639     593     438     349     325     296     271
                 231     158     140     114     102      21      10
 
PvuII CAG'CTG

Cuts at:      0     258     273     360     489     509     785    1506    1800 
   Size:        258      15      87     129      20     276     721     294

Cuts at:   1800    1877    2034    2337    2427    2636    2900    3126    3889 
   Size:         77     157     303      90     209     264     226     763

Cuts at:   3889    4199    4602 
   Size:        310     403

  Fragments arranged by size: 

                 763     721     403     310     303     294     276     264
                 258     226     209     157     129      90      87      77
                  20      15
 
RsrII CG'GwC_CG

Cuts at:      0      39    4602 
   Size:         39    4563
 
SacI G_AGCT'C

Cuts at:      0      53    4602 
   Size:         53    4549
 
SanDI GG'GwC_CC

Cuts at:      0    4383    4602 
   Size:       4383     219
 
SapI GCTCTTCn'nnn_

Cuts at:      0     718    4602 
   Size:        718    3884
 
SbfI CC_TGCA'GG

Cuts at:      0    2129    4602 
   Size:       2129    2473
 
ScaI AGT'ACT

Cuts at:      0    1477    4602 
   Size:       1477    3125
 
SexAI A'CCwGG_T

Cuts at:      0    4403    4602 
   Size:       4403     199
 
SfcI C'TryA_G

Cuts at:      0     154     268     861     993    1147    1210    2125    2356 
   Size:        154     114     593     132     154      63     915     231

Cuts at:   2356    2458    3097    3237    3258    3336    3529    3705    3967 
   Size:        102     639     140      21      78     193     176     262

Cuts at:   3967    4194    4263    4273    4602 
   Size:        227      69      10     329

  Fragments arranged by size: 

                 915     639     593     329     262     231     227     193
                 176     154     154     140     132     114     102      78
                  69      63      21      10
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      42    4602 
   Size:         42    4560
 
SfoI GGC'GCC

Cuts at:      0      82    1411    3848    4602 
   Size:         82    1329    2437     754

  Fragments arranged by size: 

                2437    1329     754      82
 
SmaI CCC'GGG

Cuts at:      0      15    4602 
   Size:         15    4587
 
SmlI C'TyrA_G

Cuts at:      0     250     417    1339    1842    2394    2833    4602 
   Size:        250     167     922     503     552     439    1769

  Fragments arranged by size: 

                1769     922     552     503     439     250     167
 
SphI G_CATG'C

Cuts at:      0    3216    4602 
   Size:       3216    1386
 
StuI AGG'CCT

Cuts at:      0     896    1136    3115    3444    4271    4300    4602 
   Size:        896     240    1979     329     827      29     302

  Fragments arranged by size: 

                1979     896     827     329     302     240      29
 
StyI C'CwwG_G

Cuts at:      0    1262    2267    2742    3806    4143    4161    4310    4602 
   Size:       1262    1005     475    1064     337      18     149     292

  Fragments arranged by size: 

                1262    1064    1005     475     337     292     149      18
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      76     733    2628    4092    4443    4602 
   Size:         76     657    1895    1464     351     159

  Fragments arranged by size: 

                1895    1464     657     351     159      76
 
TatI w'GTAC_w

Cuts at:      0    1475    2840    3611    3950    4602 
   Size:       1475    1365     771     339     652

  Fragments arranged by size: 

                1475    1365     771     652     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148    1985    2017    2310    2342    4602 
   Size:        116      32    1837      32     293      32    2260

  Fragments arranged by size: 

                2260    1837     293     116      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1531    4387    4602 
   Size:       1531    2856     215

  Fragments arranged by size: 

                2856    1531     215
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4093    4210    4602 
   Size:       4093     117     392

  Fragments arranged by size: 

                4093     392     117
 
XmaI C'CCGG_G

Cuts at:      0      13    4602 
   Size:         13    4589
 
XmnI GAAnn'nnTTC

Cuts at:      0    1318    2314    4602 
   Size:       1318     996    2288

  Fragments arranged by size: 

                2288    1318     996
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NspI     PasI     PciI    PflMI
     PfoI     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII
     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AscI     AseI
    AsiSI    AvrII     BclI    BfrBI    BglII     BmtI     BplI    BsiWI
    BspHI   BssHII    BstBI   BstEII  BstZ17I     ClaI    EcoRI    EcoRV
     FseI    FspAI     HpaI     KpnI     MfeI     MluI     NaeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI     PpiI     PsiI
    PspXI     PsrI     PvuI    SacII     SalI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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