Restriction map for pF1KSDA0789
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AocI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoRI       EcoRV       EcoT14I     FbaI        FseI        
HincII      HindII      HpaI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccI          643  GTCTAC               GT^[AC][GT]AC
AccII         915  CGCG                 CG^CG
AccIII       1568  TCCGGA               T^CCGGA
AcsI           19  AAATTC               [GA]^AATT[CT]
Alw44I       1432  GTGCAC               G^TGCAC
AlwI          552  GATCC                GGATC(4/5)
AlwNI        1049  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1556  AGCGCT               AGC^GCT
ApaI          422  GGGCCC               GGGCC^C
ApaLI        1432  GTGCAC               G^TGCAC
ApoI           19  AAATTC               [GA]^AATT[CT]
AspHI        1432  GTGCAC               G[AT]GC[AT]^C
AviII        1320  TGCGCA               TGC^GCA
BbrPI        1062  CACGTG               CAC^GTG
BbvCI         175  GCTGAGG              CCTCAGC(-5/-2)
BfaI          944  CTAG                 C^TAG
BlpI          184  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      184  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1062  CACGTG               [CT]AC^GT[GA]
BsaBI         459  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1568  TCCGGA               [AT]^CCGG[AT]
BseAI        1568  TCCGGA               T^CCGGA
BsiHKAI      1432  GTGCAC               G[AT]GC[AT]^C
BsmAI         133  GTCTC                GTCTC(1/5)
BspEI        1568  TCCGGA               T^CCGGA
BspLU11I      764  ACATGT               A^CATGT
BstEII        499  GGTTACC              G^GT[ACGT]ACC
BstPI         499  GGTTACC              G^GT[ACGT]ACC
BstUI         915  CGCG                 CG^CG
BstXI        1536  CCAGAAGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         551  AGATCC               [GA]^GATC[CT]
CelII         184  GCTGAGC              GC^T[ACGT]AGC
DraI         1147  TTTAAA               TTT^AAA
DrdI         1456  GACCTCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1375  CCGTGG               C^C[GA][CT]GG
EarI          373  GAAGAG               CTCTTC(1/4)
Eco47III     1556  AGCGCT               AGC^GCT
EcoNI        1446  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       499  GGTTACC              G^GT[ACGT]ACC
EcoT22I       208  ATGCAT               ATGCA^T
FspI         1320  TGCGCA               TGC^GCA
HindIII       307  AAGCTT               A^AGCTT
Ksp632I       373  GAAGAG               CTCTTC(1/4)
MaeI          944  CTAG                 C^TAG
MamI          459  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          551  AGATCC               [GA]^GATC[CT]
MroI         1568  TCCGGA               T^CCGGA
MseI         1148  TTAA                 T^TAA
MvnI          915  CGCG                 CG^CG
NsiI          208  ATGCAT               ATGCA^T
PaeR7I       1576  CTCGAG               C^TCGAG
PmaCI        1062  CACGTG               CAC^GTG
PmlI         1062  CACGTG               CAC^GTG
PshAI         966  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          373  GAAGAGC              GCTCTTC(1/4)
SphI         1323  GCATGC               GCATG^C
StuI         1226  AGGCCT               AGG^CCT
TfiI          863  GATTC                G^A[AT]TC
Tru9I        1148  TTAA                 T^TAA
XcmI         1385  CCACTCACACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1576  CTCGAG               C^TCGAG
XhoII         551  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII        764  ACATGT               A^C[GA][CT]GT
             1061  ACACGT              
AvaI          135  CTCGGG               C^[CT]CG[GA]G
             1576  CTCGAG              
BbeI          526  GGCGCC               GGCGC^C
              625  GGCGCC              
BbsI         1608  GAAGAC               GAAGAC(2/6)
               97  GTCTTC              
BpmI          330  CTGGAG               CTGGAG(16/14)
              517  CTGGAG              
BpuAI        1608  GAAGAC               GAAGAC(2/6)
               97  GTCTTC              
BseRI         431  GAGGAG               GAGGAG(10/8)
              919  GAGGAG              
BsoBI         135  CTCGGG               C^[CT]CG[GA]G
             1576  CTCGAG              
BspMI         260  GCAGGT               ACCTGC(4/8)
              677  GCAGGT              
BsrBI         868  CCGCTC               CCGCTC(-3/-3)
             1156  GAGCGG              
BsrDI         252  CATTGC               GCAATG(2/0)
             1029  CATTGC              
BsrFI         647  ACCGGC               [GA]^CCGG[CT]
             1514  ACCGGC              
Cfr10I        647  ACCGGC               [GA]^CCGG[CT]
             1514  ACCGGC              
DpnII         552  GATC                 ^GATC
             1221  GATC                
HgaI         1197  GACGC                GACGC(5/10)
             1234  GACGC               
KasI          526  GGCGCC               G^GCGCC
              625  GGCGCC              
MaeII         566  ACGT                 A^CGT
             1063  ACGT                
MboI          552  GATC                 ^GATC
             1221  GATC                
MslI          765  CATGTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1626  CATCAAGATG          
NarI          526  GGCGCC               GG^CGCC
              625  GGCGCC              
NdeII         552  GATC                 ^GATC
             1221  GATC                
PflMI           5  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1048  CCAGGTGCTGG         
PpuMI        1442  AGGACCT              [GA]G^G[AT]CC[CT]
             1454  AGGACCT             
PstI          161  CTGCAG               CTGCA^G
              652  CTGCAG              
Sau3AI        552  GATC                 ^GATC
             1221  GATC                
SfcI          161  CTGCAG               C^T[GA][CT]AG
              652  CTGCAG              
SfoI          526  GGCGCC               GGC^GCC
              625  GGCGCC              
Tsp45I        203  GTGAC                ^GT[GC]AC
              745  GTGAC               
Tsp509I        20  AATT                 ^AATT
             1085  AATT                
Van91I          5  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1048  CCAGGTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BmyI        BsaHI       BsaJI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeIII      MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau96I      ScrFI       SfaNI       SmlI        
TaqI        TseI        TspRI       TthHB8I     
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