Restriction map for pF1KSDA0789
 (Linear) (Six-base) MAPSORT of: KIAA0789.seq  Check: 6655  from: 1  to: 1695



KIAA0789.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:14  ..

AccI GT'mk_AC

Cuts at:      0     644    1695 
   Size:        644    1051
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1468    1695 
   Size:       1468     227
 
AfeI AGC'GCT

Cuts at:      0    1558    1695 
   Size:       1558     137
 
AflIII A'CryG_T

Cuts at:      0     764    1061    1695 
   Size:        764     297     634

  Fragments arranged by size: 

                 764     634     297
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     914    1046    1438    1695 
   Size:        914     132     392     257

  Fragments arranged by size: 

                 914     392     257     132
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     985    1017    1695 
   Size:        985      32     678

  Fragments arranged by size: 

                 985     678      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1054    1695 
   Size:       1054     641
 
ApaI G_GGCC'C

Cuts at:      0     426    1695 
   Size:        426    1269
 
ApaLI G'TGCA_C

Cuts at:      0    1432    1695 
   Size:       1432     263
 
ApoI r'AATT_y

Cuts at:      0      19    1695 
   Size:         19    1676
 
AvaI C'yCGr_G

Cuts at:      0     135    1576    1695 
   Size:        135    1441     119

  Fragments arranged by size: 

                1441     135     119
 
BanI G'GyrC_C

Cuts at:      0     526     625     829    1695 
   Size:        526      99     204     866

  Fragments arranged by size: 

                 866     526     204      99
 
BanII G_rGCy'C

Cuts at:      0     342     426    1071    1568    1695 
   Size:        342      84     645     497     127

  Fragments arranged by size: 

                 645     497     342     127      84
 
BbeI G_GCGC'C

Cuts at:      0     530     629    1695 
   Size:        530      99    1066

  Fragments arranged by size: 

                1066     530      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90    1615    1695 
   Size:         90    1525      80

  Fragments arranged by size: 

                1525      90      80
 
BbvCI CC'TCA_GC

Cuts at:      0     176    1695 
   Size:        176    1519
 
BfrBI ATG'CAT

Cuts at:      0     210    1695 
   Size:        210    1485
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1276    1310    1493    1695 
   Size:       1276      34     183     202

  Fragments arranged by size: 

                1276     202     183      34
 
BlpI GC'TnA_GC

Cuts at:      0     185    1695 
   Size:        185    1510
 
Bme1580I G_kGCm'C

Cuts at:      0     426     903    1436    1695 
   Size:        426     477     533     259

  Fragments arranged by size: 

                 533     477     426     259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     538    1695 
   Size:        351     187    1157

  Fragments arranged by size: 

                1157     351     187
 
Bpu10I CC'TnA_GC

Cuts at:      0     176    1560    1695 
   Size:        176    1384     135

  Fragments arranged by size: 

                1384     176     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     328    1390    1695 
   Size:        231      97    1062     305

  Fragments arranged by size: 

                1062     305     231      97
 
BsaAI yAC'GTr

Cuts at:      0    1064    1695 
   Size:       1064     631
 
BsaBI GATnn'nnATC

Cuts at:      0     463    1695 
   Size:        463    1232
 
BsaHI Gr'CG_yC

Cuts at:      0     527     626    1235    1695 
   Size:        527      99     609     460

  Fragments arranged by size: 

                 609     527     460      99
 
BsaWI w'CCGG_w

Cuts at:      0    1568    1695 
   Size:       1568     127
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     446     934    1695 
   Size:        446     488     761

  Fragments arranged by size: 

                 761     488     446
 
BseYI C'CCAG_C

Cuts at:      0      85     171    1470    1491    1695 
   Size:         85      86    1299      21     204

  Fragments arranged by size: 

                1299     204      86      85      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710     777     930    1695 
   Size:        710      67     153     765

  Fragments arranged by size: 

                 765     710     153      67
 
BsiHKAI G_wGCw'C

Cuts at:      0    1436    1695 
   Size:       1436     259
 
Bsp1286I G_dGCh'C

Cuts at:      0     342     426     903    1071    1436    1568    1695 
   Size:        342      84     477     168     365     132     127

  Fragments arranged by size: 

                 477     365     342     168     132     127      84
 
BspEI T'CCGG_A

Cuts at:      0    1568    1695 
   Size:       1568     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     251     668    1695 
   Size:        251     417    1027

  Fragments arranged by size: 

                1027     417     251
 
BsrBI CCG'CTC

Cuts at:      0     870    1158    1695 
   Size:        870     288     537

  Fragments arranged by size: 

                 870     537     288
 
BsrDI GCAATG_nn'

Cuts at:      0     251    1028    1695 
   Size:        251     777     667

  Fragments arranged by size: 

                 777     667     251
 
BsrFI r'CCGG_y

Cuts at:      0     647    1514    1695 
   Size:        647     867     181

  Fragments arranged by size: 

                 867     647     181
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     617     823     836    1695 
   Size:        617     206      13     859

  Fragments arranged by size: 

                 859     617     206      13
 
BstEII G'GTnAC_C

Cuts at:      0     499    1695 
   Size:        499    1196
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1543    1695 
   Size:       1543     152
 
BstYI r'GATC_y

Cuts at:      0     551    1695 
   Size:        551    1144
 
BtgI C'CryG_G

Cuts at:      0    1375    1695 
   Size:       1375     320
 
BtsI GCAGTG_nn'

Cuts at:      0     698    1695 
   Size:        698     997
 
DraI TTT'AAA

Cuts at:      0    1149    1695 
   Size:       1149     546
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1462    1695 
   Size:       1462     233
 
EaeI y'GGCC_r

Cuts at:      0       2     543    1037    1088    1209    1302    1348    1695 
   Size:          2     541     494      51     121      93      46     347

  Fragments arranged by size: 

                 541     494     347     121      93      51      46       2
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     368    1695 
   Size:        368    1327
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25    1671    1695 
   Size:         25    1646      24

  Fragments arranged by size: 

                1646      25      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     538    1468    1695 
   Size:        351     187     930     227

  Fragments arranged by size: 

                 930     351     227     187
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1450    1695 
   Size:       1450     245
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423    1443    1455    1695 
   Size:        423    1020      12     240

  Fragments arranged by size: 

                1020     423     240      12
 
FspI TGC'GCA

Cuts at:      0    1322    1695 
   Size:       1322     373
 
HaeII r_GCGC'y

Cuts at:      0     530     629    1560    1695 
   Size:        530      99     931     135

  Fragments arranged by size: 

                 931     530     135      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     854     886    1169    1201    1695 
   Size:        854      32     283      32     494

  Fragments arranged by size: 

                 854     494     283      32      32
 
HindIII A'AGCT_T

Cuts at:      0     307    1695 
   Size:        307    1388
 
KasI G'GCGC_C

Cuts at:      0     526     625    1695 
   Size:        526      99    1070

  Fragments arranged by size: 

                1070     526      99
 
MscI TGG'CCA

Cuts at:      0       4    1039    1090    1350    1695 
   Size:          4    1035      51     260     345

  Fragments arranged by size: 

                1035     345     260      51       4
 
MslI CAynn'nnrTG

Cuts at:      0     769    1630    1695 
   Size:        769     861      65

  Fragments arranged by size: 

                 861     769      65
 
MspA1I CmG'CkG

Cuts at:      0      27     130     157     175     657     751     813    1175 
   Size:         27     103      27      18     482      94      62     362

Cuts at:   1175    1470    1695 
   Size:        295     225

  Fragments arranged by size: 

                 482     362     295     225     103      94      62      27
                  27      18
 
NarI GG'CG_CC

Cuts at:      0     527     626    1695 
   Size:        527      99    1069

  Fragments arranged by size: 

                1069     527      99
 
NsiI A_TGCA'T

Cuts at:      0     212    1695 
   Size:        212    1483
 
NspI r_CATG'y

Cuts at:      0     210     768    1327    1605    1695 
   Size:        210     558     559     278      90

  Fragments arranged by size: 

                 559     558     278     210      90
 
PciI A'CATG_T

Cuts at:      0     764    1695 
   Size:        764     931
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      11    1054    1695 
   Size:         11    1043     641

  Fragments arranged by size: 

                1043     641      11
 
PfoI T'CCnGG_A

Cuts at:      0     227     359    1695 
   Size:        227     132    1336

  Fragments arranged by size: 

                1336     227     132
 
PmlI CAC'GTG

Cuts at:      0    1064    1695 
   Size:       1064     631
 
PpuMI rG'GwC_Cy

Cuts at:      0    1443    1455    1695 
   Size:       1443      12     240

  Fragments arranged by size: 

                1443     240      12
 
PshAI GACnn'nnGTC

Cuts at:      0     970    1695 
   Size:        970     725
 
PspOMI G'GGCC_C

Cuts at:      0     422    1695 
   Size:        422    1273
 
PspXI vC'TCGA_Gb

Cuts at:      0    1576    1695 
   Size:       1576     119
 
PstI C_TGCA'G

Cuts at:      0     165     656    1695 
   Size:        165     491    1039

  Fragments arranged by size: 

                1039     491     165
 
PvuII CAG'CTG

Cuts at:      0     175     657     751    1470    1695 
   Size:        175     482      94     719     225

  Fragments arranged by size: 

                 719     482     225     175      94
 
SapI GCTCTTCn'nnn_

Cuts at:      0     368    1695 
   Size:        368    1327
 
SfcI C'TryA_G

Cuts at:      0     161     652    1695 
   Size:        161     491    1043

  Fragments arranged by size: 

                1043     491     161
 
SfoI GGC'GCC

Cuts at:      0     528     627    1695 
   Size:        528      99    1068

  Fragments arranged by size: 

                1068     528      99
 
SmlI C'TyrA_G

Cuts at:      0     246     343    1405    1576    1695 
   Size:        246      97    1062     171     119

  Fragments arranged by size: 

                1062     246     171     119      97
 
SphI G_CATG'C

Cuts at:      0    1327    1695 
   Size:       1327     368
 
StuI AGG'CCT

Cuts at:      0    1228    1695 
   Size:       1228     467
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     427    1695 
   Size:        190     237    1268

  Fragments arranged by size: 

                1268     237     190
 
TatI w'GTAC_w

Cuts at:      0     386    1695 
   Size:        386    1309
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     677     709    1695 
   Size:        677      32     986

  Fragments arranged by size: 

                 986     677      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1392    1695 
   Size:       1392     303
 
XhoI C'TCGA_G

Cuts at:      0    1576    1695 
   Size:       1576     119
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
    BfrBI     BglI     BlpI Bme1580I     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI
    BstYI     BtgI     BtsI     DraI     DrdI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I  HindIII     KasI
     MscI     MslI   MspA1I     NarI     NsiI     NspI     PciI    PflMI
     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII
     SapI     SfcI     SfoI     SmlI     SphI     StuI    TaqII     TatI
     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
     BclI    BglII    BmgBI     BmrI     BmtI     BplI     BsaI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI
  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PmeI     PpiI     PsiI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XmaI     XmnI
     ZraI
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