Restriction map for pF1KSDA0753
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       BbrPI       BbvCI       BclI        
BfrI        BlpI        Bpu1102I    BsaAI       BsiEI       
BsiWI       BsoBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       BstUI       Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HpaI        KpnI        KspI        MluI        
MslI        MvnI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        Tth111I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       1341  TCCGGA               T^CCGGA
AcyI         1527  GGCGCC               G[GA]^CG[CT]C
AvrII          43  CCTAGG               C^CTAGG
BalI         1213  TGGCCA               TGG^CCA
BbeI         1527  GGCGCC               GGCGC^C
BglI          928  GCCATTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           43  CCTAGG               C^CTAGG
BsaBI        1315  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1527  GGCGCC               G[GA]^CG[CT]C
BsaI         1345  GAGACC               GGTCTC(1/5)
BsaWI        1341  TCCGGA               [AT]^CCGG[AT]
BseAI        1341  TCCGGA               T^CCGGA
BsmBI        1700  CGTCTC               CGTCTC(1/5)
BsmI          800  GAATGC               GAATGC(1/-1)
BspEI        1341  TCCGGA               T^CCGGA
BspMI         822  GCAGGT               ACCTGC(4/8)
BsrDI         895  GCAATG               GCAATG(2/0)
BsrFI        1637  ACCGGC               [GA]^CCGG[CT]
Bst1107I      517  GTATAC               GTA^TAC
BstEII        613  GGTGACC              G^GT[ACGT]ACC
BstPI         613  GGTGACC              G^GT[ACGT]ACC
BstXI          51  CCAACTTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       517  GTATAC               GTA^TAC
Cfr10I       1637  ACCGGC               [GA]^CCGG[CT]
DraIII        424  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1213  TGGCCA               [CT]^GGCC[GA]
EcoO65I       613  GGTGACC              G^GT[ACGT]ACC
EcoRI        2881  GAATTC               G^AATTC
HaeII        1527  GGCGCC               [GA]GCGC^[CT]
HgaI         1699  GCGTC                GACGC(5/10)
Hin1I        1527  GGCGCC               G[GA]^CG[CT]C
HincII       1283  GTCGAC               GT[CT]^[GA]AC
HindII       1283  GTCGAC               GT[CT]^[GA]AC
KasI         1527  GGCGCC               G^GCGCC
MamI         1315  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2002  CAATTG               C^AATTG
MluNI        1213  TGGCCA               TGG^CCA
MroI         1341  TCCGGA               T^CCGGA
MscI         1213  TGGCCA               TGG^CCA
MunI         2002  CAATTG               C^AATTG
NarI         1527  GGCGCC               GG^CGCC
NcoI         1666  CCATGG               C^CATGG
NdeI         2399  CATATG               CA^TATG
PflMI         423  CCACAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          46  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          100  CTGCAG               CTGCA^G
SalI         1283  GTCGAC               G^TCGAC
SapI         2794  GAAGAGC              GCTCTTC(1/4)
ScaI           78  AGTACT               AGT^ACT
SfoI         1527  GGCGCC               GGC^GCC
SmlI         1781  CTCAAG               C^T[CT][GA]AG
SphI         1904  GCATGC               GCATG^C
SspI         2113  AATATT               AAT^ATT
StuI         1199  AGGCCT               AGG^CCT
Van91I        423  CCACAGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI        1802  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2861  CAGAAGCTG           
BamHI         738  GGATCC               G^GATCC
             2517  GGATCC              
BbsI          329  GAAGAC               GAAGAC(2/6)
             2499  GAAGAC              
BciVI        1670  GGATAC               GTATCC(6/5)
              686  GTATCC              
BcnI          586  CCGGG                CC^[GC]GG
             2677  CCGGG               
BpuAI         329  GAAGAC               GAAGAC(2/6)
             2499  GAAGAC              
BsgI         2162  CTGCAC               GTGCAG(16/14)
             1600  GTGCAG              
BspHI        2330  TCATGA               T^CATGA
             2742  TCATGA              
CfoI         1528  GCGC                 GCG^C
             2337  GCGC                
HhaI         1528  GCGC                 GCG^C
             2337  GCGC                
HinP1I       1528  GCGC                 G^CGC
             2337  GCGC                
MaeII         753  ACGT                 A^CGT
              809  ACGT                
MspA1I         97  CAGCTG               C[AC]G^C[GT]G
             2177  CAGCTG              
NciI          586  CCGGG                CC^[GC]GG
             2677  CCGGG               
NspI          162  ACATGC               [GA]CATG^[CT]
             1904  GCATGC              
PvuII          97  CAGCTG               CAG^CTG
             2177  CAGCTG              
RcaI         2330  TCATGA               T^CATGA
             2742  TCATGA              
XbaI         1416  TCTAGA               T^CTAGA
             1945  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbvI        BcgI        BfaI        BglII       
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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