Restriction map for pF1KSDA0745
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
AspEI       AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BseAI       
BsiWI       BsoBI       BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        KspI        MluI        MroI        NaeI        
NarI        NgoMIV      NheI        NotI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         406  GACGTC               GACGT^C
Acc65I       1736  GGTACC               G^GTACC
AccII         544  CGCG                 CG^CG
AflIII        823  ACGTGT               A^C[GA][CT]GT
Alw44I        827  GTGCAC               G^TGCAC
ApaLI         827  GTGCAC               G^TGCAC
AseI          484  ATTAAT               AT^TAAT
AsnI          484  ATTAAT               AT^TAAT
Asp718I      1736  GGTACC               G^GTACC
AspI          379  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1097  TGGCCA               TGG^CCA
BanI         1736  GGTACC               G^G[CT][GA]CC
BanII        1729  GAGCTC               G[GA]GC[CT]^C
BcnI          972  CCGGG                CC^[GC]GG
BglII        1130  AGATCT               A^GATCT
BsaAI        2306  CACGTA               [CT]AC^GT[GA]
BsaBI        1501  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2538  ACCGGA               [AT]^CCGG[AT]
BsmI         2644  GCATTC               GAATGC(1/-1)
Bsp1407I      730  TGTACA               T^GTACA
BspHI        1670  TCATGA               T^CATGA
BsrGI         730  TGTACA               T^GTACA
BstUI         544  CGCG                 CG^CG
BstYI        1130  AGATCT               [GA]^GATC[CT]
DraIII       2275  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          289  CGGCCG               C^GGCCG
EclXI         289  CGGCCG               C^GGCCG
Eco52I        289  CGGCCG               C^GGCCG
EcoRI        2676  GAATTC               G^AATTC
EcoRV         562  GATATC               GAT^ATC
FauI         1075  GCGGG                CCCGC(4/6)
HaeII         886  AGCGCC               [GA]GCGC^[CT]
HincII       2659  GTCAAC               GT[CT]^[GA]AC
HindII       2659  GTCAAC               GT[CT]^[GA]AC
KpnI         1736  GGTACC               GGTAC^C
MamI         1501  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1724  CAATTG               C^AATTG
MflI         1130  AGATCT               [GA]^GATC[CT]
MluNI        1097  TGGCCA               TGG^CCA
MscI         1097  TGGCCA               TGG^CCA
MslI          909  CATTGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1724  CAATTG               C^AATTG
MvnI          544  CGCG                 CG^CG
NciI          972  CCGGG                CC^[GC]GG
NdeI         2155  CATATG               CA^TATG
NruI          543  TCGCGA               TCG^CGA
PflFI         379  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1209  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         484  ATTAAT               AT^TAAT
PstI         2047  CTGCAG               CTGCA^G
RcaI         1670  TCATGA               T^CATGA
SacI         1729  GAGCTC               GAGCT^C
ScaI         1256  AGTACT               AGT^ACT
SspBI         730  TGTACA               T^GTACA
Tth111I       379  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1130  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          106  GAATTT               [GA]^AATT[CT]
             2676  GAATTC              
ApoI          106  GAATTT               [GA]^AATT[CT]
             2676  GAATTC              
Asp700I      1117  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1783  GAATTGGTTC          
BlpI         1104  GCTGAGC              GC^T[ACGT]AGC
             2109  GCTCAGC             
Bpu1102I     1104  GCTGAGC              GC^T[ACGT]AGC
             2109  GCTCAGC             
BsaI         1207  GAGACC               GGTCTC(1/5)
             1625  GAGACC              
BsiEI         289  CGGCCG               CG[GA][CT]^CG
             2349  CGACCG              
BsrDI         909  CATTGC               GCAATG(2/0)
             2699  GCAATG              
BssSI        1383  CACGAG               CACGAG(-5/-1)
              835  CTCGTG              
CelII        1104  GCTGAGC              GC^T[ACGT]AGC
             2109  GCTCAGC             
CfoI          887  GCGC                 GCG^C
              893  GCGC                
DraII        1798  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1907  AGGTCCC             
EcoNI        2142  CCTGACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2334  CCTGAACCAGG         
EcoO109I     1798  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1907  AGGTCCC             
HapII         972  CCGG                 C^CGG
             2539  CCGG                
HhaI          887  GCGC                 GCG^C
              893  GCGC                
HinP1I        887  GCGC                 G^CGC
              893  GCGC                
HindIII       205  AAGCTT               A^AGCTT
             1348  AAGCTT              
HpaII         972  CCGG                 C^CGG
             2539  CCGG                
MspI          972  CCGG                 C^CGG
             2539  CCGG                
NspI          193  ACATGC               [GA]CATG^[CT]
             1436  ACATGC              
PpuMI        1798  AGGTCCC              [GA]G^G[AT]CC[CT]
             1907  AGGTCCC             
XmnI         1117  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1783  GAATTGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HgaI        Hin1I       HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      
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