Restriction map for pF1KSDA0745
 (Linear) (Six-base) MAPSORT of: KIAA0745.seq  Check: 1906  from: 1  to: 2715



KIAA0745.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AatII G_ACGT'C

Cuts at:      0     410    2715 
   Size:        410    2305
 
Acc65I G'GTAC_C

Cuts at:      0    1736    2715 
   Size:       1736     979
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     584    1954    1990    2237    2715 
   Size:        372     212    1370      36     247     478

  Fragments arranged by size: 

                1370     478     372     247     212      36
 
AflIII A'CryG_T

Cuts at:      0     823    2715 
   Size:        823    1892
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     210    2715 
   Size:        210    2505
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     183     876    2400    2637    2715 
   Size:         21     162     693    1524     237      78

  Fragments arranged by size: 

                1524     693     237     162      78      21
 
ApaLI G'TGCA_C

Cuts at:      0     827    2715 
   Size:        827    1888
 
ApoI r'AATT_y

Cuts at:      0     106    2676    2715 
   Size:        106    2570      39

  Fragments arranged by size: 

                2570     106      39
 
AseI AT'TA_AT

Cuts at:      0     485    2715 
   Size:        485    2230
 
BanI G'GyrC_C

Cuts at:      0    1736    2715 
   Size:       1736     979
 
BanII G_rGCy'C

Cuts at:      0    1733    2715 
   Size:       1733     982
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     378     826    1218    2438    2715 
   Size:        378     448     392    1220     277

  Fragments arranged by size: 

                1220     448     392     378     277
 
BglII A'GATC_T

Cuts at:      0    1130    2715 
   Size:       1130    1585
 
BlpI GC'TnA_GC

Cuts at:      0    1105    2110    2715 
   Size:       1105    1005     605

  Fragments arranged by size: 

                1105    1005     605
 
Bme1580I G_kGCm'C

Cuts at:      0     831    2715 
   Size:        831    1884
 
BmgBI CAC'GTC

Cuts at:      0     824    2187    2715 
   Size:        824    1363     528

  Fragments arranged by size: 

                1363     824     528
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     171     361     855    1269    2715 
   Size:        171     190     494     414    1446

  Fragments arranged by size: 

                1446     494     414     190     171
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     774    1021    1795    2715 
   Size:         67     707     247     774     920

  Fragments arranged by size: 

                 920     774     707     247      67
 
Bpu10I CC'TnA_GC

Cuts at:      0     130    1219    2715 
   Size:        130    1089    1496

  Fragments arranged by size: 

                1496    1089     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    1753    1924    2715 
   Size:        643    1110     171     791

  Fragments arranged by size: 

                1110     791     643     171
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1201    1619    2715 
   Size:       1201     418    1096

  Fragments arranged by size: 

                1201    1096     418
 
BsaAI yAC'GTr

Cuts at:      0    2308    2715 
   Size:       2308     407
 
BsaBI GATnn'nnATC

Cuts at:      0    1505    2715 
   Size:       1505    1210
 
BsaHI Gr'CG_yC

Cuts at:      0     407    1004    1473    2715 
   Size:        407     597     469    1242

  Fragments arranged by size: 

                1242     597     469     407
 
BsaWI w'CCGG_w

Cuts at:      0    2538    2715 
   Size:       2538     177
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     823    1468    2059    2715 
   Size:        139     684     645     591     656

  Fragments arranged by size: 

                 684     656     645     591     139
 
BseYI C'CCAG_C

Cuts at:      0    1802    2715 
   Size:       1802     913
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     757     859     937    1171    2213    2344    2715 
   Size:         34     723     102      78     234    1042     131     371

  Fragments arranged by size: 

                1042     723     371     234     131     102      78      34
            
 
BsiEI CG_ry'CG

Cuts at:      0     292    2352    2715 
   Size:        292    2060     363

  Fragments arranged by size: 

                2060     363     292
 
BsiHKAI G_wGCw'C

Cuts at:      0     272     831    1037    1394    1733    2715 
   Size:        272     559     206     357     339     982

  Fragments arranged by size: 

                 982     559     357     339     272     206
 
BsmI GAATG_Cn'

Cuts at:      0    2644    2715 
   Size:       2644      71
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1480    2018    2020    2715 
   Size:       1480     538       2     695

  Fragments arranged by size: 

                1480     695     538       2
 
Bsp1286I G_dGCh'C

Cuts at:      0     272     831    1037    1394    1733    2715 
   Size:        272     559     206     357     339     982

  Fragments arranged by size: 

                 982     559     357     339     272     206
 
BspHI T'CATG_A

Cuts at:      0    1670    2715 
   Size:       1670    1045
 
BsrDI GCAATG_nn'

Cuts at:      0     908    2706    2715 
   Size:        908    1798       9

  Fragments arranged by size: 

                1798     908       9
 
BsrGI T'GTAC_A

Cuts at:      0     730    2715 
   Size:        730    1985
 
BssSI C'ACGA_G

Cuts at:      0     835    1383    2715 
   Size:        835     548    1332

  Fragments arranged by size: 

                1332     835     548
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     642    1524    2255    2367    2556    2715 
   Size:        642     882     731     112     189     159

  Fragments arranged by size: 

                 882     731     642     189     159     112
 
BstYI r'GATC_y

Cuts at:      0    1130    2715 
   Size:       1130    1585
 
BtgI C'CryG_G

Cuts at:      0    1007    1211    2240    2405    2715 
   Size:       1007     204    1029     165     310

  Fragments arranged by size: 

                1029    1007     310     204     165
 
BtsI GCAGTG_nn'

Cuts at:      0     920     965    2715 
   Size:        920      45    1750

  Fragments arranged by size: 

                1750     920      45
 
DraIII CAC_nnn'GTG

Cuts at:      0    2280    2715 
   Size:       2280     435
 
EaeI y'GGCC_r

Cuts at:      0     289     969    1097    2715 
   Size:        289     680     128    1618

  Fragments arranged by size: 

                1618     680     289     128
 
EagI C'GGCC_G

Cuts at:      0     289    2715 
   Size:        289    2426
 
EarI CTCTTCn'nnn_

Cuts at:      0     540    1830    1852    2211    2296    2314    2715 
   Size:        540    1290      22     359      85      18     401

  Fragments arranged by size: 

                1290     540     401     359      85      22      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    2715 
   Size:         19    2696
 
EcoICRI GAG'CTC

Cuts at:      0    1731    2715 
   Size:       1731     984
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     372     584     774    1021    1795    1954    1990 
   Size:         67     305     212     190     247     774     159      36

Cuts at:   1990    2237    2715 
   Size:        247     478

  Fragments arranged by size: 

                 774     478     305     247     247     212     190     159
                  67      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2146    2338    2715 
   Size:       2146     192     377

  Fragments arranged by size: 

                2146     377     192
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1799    1908    2715 
   Size:       1799     109     807

  Fragments arranged by size: 

                1799     807     109
 
EcoRI G'AATT_C

Cuts at:      0    2676    2715 
   Size:       2676      39
 
EcoRV GAT'ATC

Cuts at:      0     564    2715 
   Size:        564    2151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     185    1683    2715 
   Size:        185    1498    1032

  Fragments arranged by size: 

                1498    1032     185
 
HaeII r_GCGC'y

Cuts at:      0     890    2715 
   Size:        890    1825
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     700     732     947     979    2715 
   Size:        700      32     215      32    1736

  Fragments arranged by size: 

                1736     700     215      32      32
 
HincII GTy'rAC

Cuts at:      0    2661    2715 
   Size:       2661      54
 
HindIII A'AGCT_T

Cuts at:      0     205    1348    2715 
   Size:        205    1143    1367

  Fragments arranged by size: 

                1367    1143     205
 
KpnI G_GTAC'C

Cuts at:      0    1740    2715 
   Size:       1740     975
 
MfeI C'AATT_G

Cuts at:      0    1724    2715 
   Size:       1724     991
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1426    1448    1740    2715 
   Size:       1426      22     292     975

  Fragments arranged by size: 

                1426     975     292      22
 
MscI TGG'CCA

Cuts at:      0    1099    2715 
   Size:       1099    1616
 
MslI CAynn'nnrTG

Cuts at:      0     913    2715 
   Size:        913    1802
 
MspA1I CmG'CkG

Cuts at:      0      51     752     789    1041    2301    2715 
   Size:         51     701      37     252    1260     414

  Fragments arranged by size: 

                1260     701     414     252      51      37
 
NcoI C'CATG_G

Cuts at:      0    1007    1211    2240    2715 
   Size:       1007     204    1029     475

  Fragments arranged by size: 

                1029    1007     475     204
 
NdeI CA'TA_TG

Cuts at:      0    2156    2715 
   Size:       2156     559
 
NruI TCG'CGA

Cuts at:      0     545    2715 
   Size:        545    2170
 
NspI r_CATG'y

Cuts at:      0     197    1440    2715 
   Size:        197    1243    1275

  Fragments arranged by size: 

                1275    1243     197
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     183     752    2470    2715 
   Size:        183     569    1718     245

  Fragments arranged by size: 

                1718     569     245     183
 
PpuMI rG'GwC_Cy

Cuts at:      0    1799    1908    2715 
   Size:       1799     109     807

  Fragments arranged by size: 

                1799     807     109
 
PshAI GACnn'nnGTC

Cuts at:      0    1213    2715 
   Size:       1213    1502
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1504    1536    2715 
   Size:       1504      32    1179

  Fragments arranged by size: 

                1504    1179      32
 
PstI C_TGCA'G

Cuts at:      0    2051    2715 
   Size:       2051     664
 
PvuII CAG'CTG

Cuts at:      0      51     752     789    1041    2301    2715 
   Size:         51     701      37     252    1260     414

  Fragments arranged by size: 

                1260     701     414     252      51      37
 
SacI G_AGCT'C

Cuts at:      0    1733    2715 
   Size:       1733     982
 
ScaI AGT'ACT

Cuts at:      0    1258    2715 
   Size:       1258    1457
 
SfcI C'TryA_G

Cuts at:      0     880    1294    2047    2436    2715 
   Size:        880     414     753     389     279

  Fragments arranged by size: 

                 880     753     414     389     279
 
SmlI C'TyrA_G

Cuts at:      0     658    1768    1939    2715 
   Size:        658    1110     171     776

  Fragments arranged by size: 

                1110     776     658     171
 
StyI C'CwwG_G

Cuts at:      0      98    1007    1211    2240    2715 
   Size:         98     909     204    1029     475

  Fragments arranged by size: 

                1029     909     475     204      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     517    2644    2715 
   Size:        517    2127      71

  Fragments arranged by size: 

                2127     517      71
 
TatI w'GTAC_w

Cuts at:      0     730    1256    1753    1764    2715 
   Size:        730     526     497      11     951

  Fragments arranged by size: 

                 951     730     526     497      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     489     521    2715 
   Size:        489      32    2194

  Fragments arranged by size: 

                2194     489      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     382    2715 
   Size:        382    2333
 
XmnI GAAnn'nnTTC

Cuts at:      0    1121    1787    2715 
   Size:       1121     666     928

  Fragments arranged by size: 

                1121     928     666
 
ZraI GAC'GTC

Cuts at:      0     408    2715 
   Size:        408    2307
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI   AflIII     AlfI    AlwNI    ApaLI     ApoI
     AseI     BanI    BanII     BbsI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrDI    BsrGI    BssSI   BstAPI    BstYI     BtgI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NruI     NspI    PflMI    PpuMI    PshAI     PsrI     PstI
    PvuII     SacI     ScaI     SfcI     SmlI     StyI    TaqII     TatI
     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BmtI
     BplI    BsaXI    BsiWI    BspEI    BspMI    BsrBI    BsrFI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     FseI     FspI    FspAI     HpaI     KasI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI     XmaI
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