Restriction map for pF1KSDA0712
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
BbeI        BbrPI       BfrI        BglI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsiWI       BsmBI       
BspDI       BspLU11I    BsrFI       BssSI       BstBI       
BstEII      BstPI       CelII       Cfr10I      ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       FseI        
Hin1I       HpaI        KasI        KpnI        KspI        
MluI        NaeI        NarI        NgoMIV      NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII        3145  CGCG                 CG^CG
AhdI         3019  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        3019  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3162  TGCGCA               TGC^GCA
AvrII        4033  CCTAGG               C^CTAGG
BamHI        2337  GGATCC               G^GATCC
BcgI         3608  GCAGCCGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2484  TGATCA               T^GATCA
BlnI         4033  CCTAGG               C^CTAGG
BsiEI        2253  CGATCG               CG[GA][CT]^CG
Bsp1407I     4055  TGTACA               T^GTACA
BspHI        1218  TCATGA               T^CATGA
BsrBI        3837  CCGCTC               CCGCTC(-3/-3)
BsrDI        3314  CATTGC               GCAATG(2/0)
BsrGI        4055  TGTACA               T^GTACA
BssHII       3144  GCGCGC               G^CGCGC
Bst1107I     2157  GTATAC               GTA^TAC
BstAPI       1153  GCACTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        3145  CGCG                 CG^CG
BstZ17I      2157  GTATAC               GTA^TAC
DraIII       3502  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         5004  GACTGTTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     3019  GACCCTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         2484  TGATCA               T^GATCA
FspI         3162  TGCGCA               TGC^GCA
HgaI         3025  GCGTC                GACGC(5/10)
HincII       1294  GTTGAC               GT[CT]^[GA]AC
HindII       1294  GTTGAC               GT[CT]^[GA]AC
HindIII      4549  AAGCTT               A^AGCTT
MvnI         3145  CGCG                 CG^CG
NheI         2085  GCTAGC               G^CTAGC
PaeR7I       3005  CTCGAG               C^TCGAG
PshAI         458  GACACTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         2253  CGATCG               CGAT^CG
RcaI         1218  TCATGA               T^CATGA
SacI         3636  GAGCTC               GAGCT^C
SapI         1116  GCTCTTC              GCTCTTC(1/4)
SexAI        1886  ACCAGGT              A^CC[AT]GGT
SspBI        4055  TGTACA               T^GTACA
XbaI         2229  TCTAGA               T^CTAGA
XhoI         3005  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         2157  GTATAC               GT^[AC][GT]AC
             2386  GTAGAC              
AccIII       3692  TCCGGA               T^CCGGA
             4217  TCCGGA              
ApaI         3947  GGGCCC               GGGCC^C
             4633  GGGCCC              
Asp700I      2447  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3667  GAAGACATTC          
BalI          671  TGGCCA               TGG^CCA
             4656  TGGCCA              
BanI         3118  GGTGCC               G^G[CT][GA]CC
             3514  GGTGCC              
BbvCI        5050  CCTCAGC              CCTCAGC(-5/-2)
             1285  GCTGAGG             
BciVI        1420  GGATAC               GTATCC(6/5)
              721  GTATCC              
BglII        1138  AGATCT               A^GATCT
             2890  AGATCT              
BsaBI         390  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1333  GATTTTGATC          
BsaI         2133  GAGACC               GGTCTC(1/5)
             3987  GAGACC              
BsaWI        3692  TCCGGA               [AT]^CCGG[AT]
             4217  TCCGGA              
BseAI        3692  TCCGGA               T^CCGGA
             4217  TCCGGA              
BsmI         1749  GAATGC               GAATGC(1/-1)
              133  GCATTC              
BspEI        3692  TCCGGA               T^CCGGA
             4217  TCCGGA              
HaeII        2082  GGCGCT               [GA]GCGC^[CT]
             4988  AGCGCC              
MamI          390  GATAAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1333  GATTTTGATC          
MfeI          518  CAATTG               C^AATTG
              794  CAATTG              
MluNI         671  TGGCCA               TGG^CCA
             4656  TGGCCA              
MroI         3692  TCCGGA               T^CCGGA
             4217  TCCGGA              
MscI          671  TGGCCA               TGG^CCA
             4656  TGGCCA              
MunI          518  CAATTG               C^AATTG
              794  CAATTG              
NdeI         1859  CATATG               CA^TATG
             3560  CATATG              
SmaI         3122  CCCGGG               CCC^GGG
             4085  CCCGGG              
XmaCI        3122  CCCGGG               C^CCGGG
             4085  CCCGGG              
XmaI         3122  CCCGGG               C^CCGGG
             4085  CCCGGG              
XmnI         2447  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3667  GAAGACATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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