Restriction map for pF1KSDA0712
 (Circular) (Six-base) MAPSORT of: pF1KSDA0712.seq  Check: 4931  from: 1  to: 8353



pF1KSDA0712  8353 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3275    3275 
   Size:       8353
 
AccI GT'mk_AC

Cuts at:   2284    2513    5389    2284 
   Size:        229    2876    5248

  Fragments arranged by size: 

                5248    2876     229
 
Acc65I G'GTAC_C

Cuts at:   5367    5367 
   Size:       8353
 
AclI AA'CG_TT

Cuts at:   8193    8193 
   Size:       8353
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     681     955    1115    1636    1718    2015    2045    2339 
   Size:        506     274     160     521      82     297      30     294

Cuts at:   2339    2564    2648    2950    3973    6176    6608    7357    7625 
   Size:        225      84     302    1023    2203     432     749     268

Cuts at:   7625     175 
   Size:        903

  Fragments arranged by size: 

                2203    1023     903     749     521     506     432     302
                 297     294     274     268     225     160      84      82
                  30
 
AflIII A'CryG_T

Cuts at:   5703    6809    7786    5703 
   Size:       1106     977    6270

  Fragments arranged by size: 

                6270    1106     977
 
AgeI A'CCGG_T

Cuts at:   6741    6741 
   Size:       8353
 
AhdI GACnn_n'nnGTC

Cuts at:   3150    3150 
   Size:       8353
 
AleI CACnn'nnGTG

Cuts at:   3427    3688    3427 
   Size:        261    8092
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    817    4964     817 
   Size:       4147    4206
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2973    3005    2973 
   Size:         32    8321
 
AlwNI CAG_nnn'CTG

Cuts at:    153    2007    2458    4471    4973    5656    7225     153 
   Size:       1854     451    2013     502     683    1569    1281

  Fragments arranged by size: 

                2013    1854    1569    1281     683     502     451
 
ApaI G_GGCC'C

Cuts at:   4077    4763    4077 
   Size:        686    7667
 
ApaLI G'TGCA_C

Cuts at:   7123    7123 
   Size:       8353
 
ApoI r'AATT_y

Cuts at:    216     340     472     979    2764    5355    7706    7962     216 
   Size:        124     132     507    1785    2591    2351     256     607

  Fragments arranged by size: 

                2591    2351    1785     607     507     256     132     124
            
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       8353
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8353
 
AvaI C'yCGr_G

Cuts at:   3131    3248    4211    4540    4573    5371    3131 
   Size:        117     963     329      33     798    6113

  Fragments arranged by size: 

                6113     963     798     329     117      33
 
AvrII C'CTAG_G

Cuts at:   4159    4159 
   Size:       8353
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3913    3946    3913 
   Size:         33    8320
 
BamHI G'GATC_C

Cuts at:   2463    5376    2463 
   Size:       2913    5440
 
BanI G'GyrC_C

Cuts at:   3244    3640    5367    5781    6029    6064    3244 
   Size:        396    1727     414     248      35    5533

  Fragments arranged by size: 

                5533    1727     414     396     248      35
 
BanII G_rGCy'C

Cuts at:    657    1432    1693    3766    4077    4596    4763    5365    6395 
   Size:        775     261    2073     311     519     167     602    1030

Cuts at:   6395     657 
   Size:       2615

  Fragments arranged by size: 

                2615    2073    1030     775     602     519     311     261
                 167
 
BbeI G_GCGC'C

Cuts at:   5785    6033    5785 
   Size:        248    8105
 
BbsI GAAGACnn'nnnn_

Cuts at:   1263    2636    3664    3746    3800    3842    4690    1263 
   Size:       1373    1028      82      54      42     848    4926

  Fragments arranged by size: 

                4926    1373    1028     848      82      54      42
 
BbvCI CC'TCA_GC

Cuts at:   1412    5177    1412 
   Size:       3765    4588
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3723    3757    3723 
   Size:         34    8319
 
BciVI GTATCCnnnnn_n'

Cuts at:    858    1540    6249    7012    8353     858 
   Size:        682    4709     763    1341     858

  Fragments arranged by size: 

                4709    1341     858     763     682
 
BclI T'GATC_A

Cuts at:   2610    5871    2610 
   Size:       3261    5092
 
BfrBI ATG'CAT

Cuts at:    627    1879    3439     627 
   Size:       1252    1560    5541

  Fragments arranged by size: 

                5541    1560    1252
 
BglII A'GATC_T

Cuts at:   1264    3016    5866    7905    1264 
   Size:       1752    2850    2039    1712

  Fragments arranged by size: 

                2850    2039    1752    1712
 
BlpI GC'TnA_GC

Cuts at:   5463    5463 
   Size:       8353
 
Bme1580I G_kGCm'C

Cuts at:   3249    4077    4716    4763    5228    5976    6069    7127    3249 
   Size:        828     639      47     465     748      93    1058    4475

  Fragments arranged by size: 

                4475    1058     828     748     639     465      93      47
            
 
BmgBI CAC'GTC

Cuts at:    357    4207     357 
   Size:       3850    4503
 
BmrI ACTGGGnnnn_n'

Cuts at:   2468    5075    5136    5563    5644    5979    8125    2468 
   Size:       2607      61     427      81     335    2146    2696

  Fragments arranged by size: 

                2696    2607    2146     427     335      81      61
 
BmtI G_CTAG'C

Cuts at:   2215    2215 
   Size:       8353
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3617    4376    4980    6718    3617 
   Size:        759     604    1738    5252

  Fragments arranged by size: 

                5252    1738     759     604
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    589    1534    1598    2389    2864    3348    4868    4950     589 
   Size:        945      64     791     475     484    1520      82    3992

  Fragments arranged by size: 

                3992    1520     945     791     484     475      82      64
            
 
Bpu10I CC'TnA_GC

Cuts at:    905    1412    3470    4703    5177     905 
   Size:        507    2058    1233     474    4081

  Fragments arranged by size: 

                4081    2058    1233     507     474
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1446    1707    2049    4330    5527    6406    6900    7198    1446 
   Size:        261     342    2281    1197     879     494     298    2601

  Fragments arranged by size: 

                2601    2281    1197     879     494     342     298     261
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   2253    4107    8057    2253 
   Size:       1854    3950    2549

  Fragments arranged by size: 

                3950    2549    1854
 
BsaAI yAC'GTr

Cuts at:   5343    5704    5343 
   Size:        361    7992
 
BsaBI GATnn'nnATC

Cuts at:      6     520    1463    5865    7904       6 
   Size:        514     943    4402    2039     455

  Fragments arranged by size: 

                4402    2039     943     514     455
 
BsaHI Gr'CG_yC

Cuts at:   5782    6030    6734    8230    5782 
   Size:        248     704    1496    5905

  Fragments arranged by size: 

                5905    1496     704     248
 
BsaWI w'CCGG_w

Cuts at:   3818    4343    5540    6061    6741    7015    7162    7573    3818 
   Size:        525    1197     521     680     274     147     411    4598

  Fragments arranged by size: 

                4598    1197     680     525     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2862    2892    2862 
   Size:         30    8323
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1213    1375    1391    1637    3489    3756    4680    5046    5204 
   Size:        162      16     246    1852     267     924     366     158

Cuts at:   5204    1213 
   Size:       4362

  Fragments arranged by size: 

                4362    1852     924     366     267     246     162     158
                  16
 
BseYI C'CCAG_C

Cuts at:   1308    1386    2278    2915    5776    6534    7113    7609    1308 
   Size:         78     892     637    2861     758     579     496    2052

  Fragments arranged by size: 

                2861    2052     892     758     637     579     496      78
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    237     950    3247    3293    3392    3847    4438     237 
   Size:        713    2297      46      99     455     591    4152

  Fragments arranged by size: 

                4152    2297     713     591     455      99      46
 
BsiEI CG_ry'CG

Cuts at:    104    2382    5893    5939    7149     104 
   Size:       2278    3511      46    1210    1308

  Fragments arranged by size: 

                3511    2278    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    209     356    2902    3766    4362    4709    5365    6143    6333 
   Size:        147    2546     864     596     347     656     778     190

Cuts at:   6333    7127     209 
   Size:        794    1435

  Fragments arranged by size: 

                2546    1435     864     794     778     656     596     347
                 190     147
 
BsmI GAATG_Cn'

Cuts at:    259    1881     259 
   Size:       1622    6731
 
Bsp1286I G_dGCh'C

Cuts at:    209     356     657    1432    1693    2902    3249    3766    4077 
   Size:        147     301     775     261    1209     347     517     311

Cuts at:   4077    4362    4596    4709    4716    4763    5228    5365    5976 
   Size:        285     234     113       7      47     465     137     611

Cuts at:   5976    6069    6143    6333    6395    7127     209 
   Size:         93      74     190      62     732    1435

  Fragments arranged by size: 

                1435    1209     775     732     611     517     465     347
                 311     301     285     261     234     190     147     137
                 113      93      74      62      47       7
 
BspEI T'CCGG_A

Cuts at:   3818    4343    7573    3818 
   Size:        525    3230    4598

  Fragments arranged by size: 

                4598    3230     525
 
BspHI T'CATG_A

Cuts at:   1344    7529    1344 
   Size:       6185    2168
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    228     969    3036    3275    5401    5615    5917    6298     228 
   Size:        741    2067     239    2126     214     302     381    2283

  Fragments arranged by size: 

                2283    2126    2067     741     381     302     239     214
            
 
BsrBI CCG'CTC

Cuts at:     50    3965    6643    6697    8183    8348      50 
   Size:       3915    2678      54    1486     165      55

  Fragments arranged by size: 

                3915    2678    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3439    6263    3439 
   Size:       2824    5529
 
BsrFI r'CCGG_y

Cuts at:   6349    6530    6741    6349 
   Size:        181     211    7961

  Fragments arranged by size: 

                7961     211     181
 
BsrGI T'GTAC_A

Cuts at:   4181    4181 
   Size:       8353
 
BssHII G'CGCG_C

Cuts at:   3270    3270 
   Size:       8353
 
BssSI C'ACGA_G

Cuts at:   6622    6982    6622 
   Size:        360    7993
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1285    5728    1285 
   Size:       4443    3910
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       8353
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3333    3429    3637    4780    4846    4920    3333 
   Size:         96     208    1143      66      74    6766

  Fragments arranged by size: 

                6766    1143     208      96      74      66
 
BstYI r'GATC_y

Cuts at:      1    1264    1930    2463    3016    4305    4436    4467    5327 
   Size:       1263     666     533     553    1289     131      31     860

Cuts at:   5327    5376    5843    5866    6201    6447    7461    7547    7559 
   Size:         49     467      23     335     246    1014      86      12

Cuts at:   7559    7905       1 
   Size:        346     449

  Fragments arranged by size: 

                1289    1263    1014     860     666     553     533     467
                 449     346     335     246     131      86      49      31
                  23      12
 
BstZ17I GTA'TAC

Cuts at:   2285    2285 
   Size:       8353
 
Bsu36I CC'TnA_GG

Cuts at:   1493    2132    4663    1493 
   Size:        639    2531    5183

  Fragments arranged by size: 

                5183    2531     639
 
BtgI C'CryG_G

Cuts at:   1145    1828    3137    3197    3665    4611    1145 
   Size:        683    1309      60     468     946    4887

  Fragments arranged by size: 

                4887    1309     946     683     468      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   6301    6482    6645    6301 
   Size:        181     163    8009

  Fragments arranged by size: 

                8009     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2939    3313    3375    3410    5130    5594    2939 
   Size:        374      62      35    1720     464    5698

  Fragments arranged by size: 

                5698    1720     464     374      62      35
 
DraI TTT'AAA

Cuts at:   5350    5350 
   Size:       8353
 
DraIII CAC_nnn'GTG

Cuts at:   3633    3633 
   Size:       8353
 
DrdI GACnn_nn'nnGTC

Cuts at:   5136    6057    6917    5136 
   Size:        921     860    6572

  Fragments arranged by size: 

                6572     921     860
 
EaeI y'GGCC_r

Cuts at:    797    4008    4782    5936    6110    6501    6528    7875     797 
   Size:       3211     774    1154     174     391      27    1347    1275

  Fragments arranged by size: 

                3211    1347    1275    1154     774     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   5936    5936 
   Size:       8353
 
EarI CTCTTCn'nnn_

Cuts at:    599     875    1249    1655    2251    2831    6374    6584     599 
   Size:        276     374     406     596     580    3543     210    2368

  Fragments arranged by size: 

                3543    2368     596     580     406     374     276     210
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2993    3833    6559    6871    7017    8163    2993 
   Size:        840    2726     312     146    1146    3183

  Fragments arranged by size: 

                3183    2726    1146     840     312     146
 
EcoICRI GAG'CTC

Cuts at:   3764    5363    3764 
   Size:       1599    6754
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    175     589     681     955    1115    1534    1598    1636    1718 
   Size:        414      92     274     160     419      64      38      82

Cuts at:   1718    2015    2045    2339    2389    2564    2648    2864    2950 
   Size:        297      30     294      50     175      84     216      86

Cuts at:   2950    3348    3973    4868    4950    6176    6608    7357    7625 
   Size:        398     625     895      82    1226     432     749     268

Cuts at:   7625     175 
   Size:        903

  Fragments arranged by size: 

                1226     903     895     749     625     432     419     414
                 398     297     294     274     268     216     175     160
                  92      86      84      82      82      64      50      38
                  30
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2011    3507    3771    4935    2011 
   Size:       1496     264    1164    5429

  Fragments arranged by size: 

                5429    1496    1164     264
 
EcoO109I rG'GnC_Cy

Cuts at:    958    2267    2556    2828    3252    4073    4074    4378    4760 
   Size:       1309     289     272     424     821       1     304     382

Cuts at:   4760    5076    5255    5282    5490     958 
   Size:        316     179      27     208    3821

  Fragments arranged by size: 

                3821    1309     821     424     382     316     304     289
                 272     208     179      27       1
 
EcoRI G'AATT_C

Cuts at:   5355    5355 
   Size:       8353
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    441    6370     441 
   Size:       5929    2424
 
FspI TGC'GCA

Cuts at:   3290    6132    3290 
   Size:       2842    5511
 
HaeII r_GCGC'y

Cuts at:   2212    5118    5785    6033    7057    8036    2212 
   Size:       2906     667     248    1024     979    2529

  Fragments arranged by size: 

                2906    2529    1024     979     667     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    263     295    1267    1299    1456    1488    1693    1725    2041 
   Size:         32     972      32     157      32     205      32     316

Cuts at:   2041    2073    2314    2346    2862    2894    3136    3168    3736 
   Size:         32     241      32     516      32     242      32     568

Cuts at:   3736    3768    4621    4653    4783    4815    5152    5184    6193 
   Size:         32     853      32     130      32     337      32    1009

Cuts at:   6193    6223    6225    6255     263 
   Size:         30       2      30    2361

  Fragments arranged by size: 

                2361    1009     972     853     568     516     337     316
                 242     241     205     157     130      32      32      32
                  32      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1422    5390    1422 
   Size:       3968    4385
 
HindIII A'AGCT_T

Cuts at:   4675    4675 
   Size:       8353
 
KasI G'GCGC_C

Cuts at:   5781    6029    5781 
   Size:        248    8105
 
KpnI G_GTAC'C

Cuts at:   5371    5371 
   Size:       8353
 
MfeI C'AATT_G

Cuts at:    644     920     644 
   Size:        276    8077
 
MluI A'CGCG_T

Cuts at:   7786    7786 
   Size:       8353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    163    1272    2501    7024    7208     163 
   Size:       1109    1229    4523     184    1308

  Fragments arranged by size: 

                4523    1308    1229    1109     184
 
MscI TGG'CCA

Cuts at:    799    4784    6112     799 
   Size:       3985    1328    3040

  Fragments arranged by size: 

                3985    3040    1328
 
MslI CAynn'nnrTG

Cuts at:    622     721     767     799    1349    1479    2305    3296    3328 
   Size:         99      46      32     550     130     826     991      32

Cuts at:   3328    3427    3688    6340    6467     622 
   Size:         99     261    2652     127    2508

  Fragments arranged by size: 

                2652    2508     991     826     550     261     130     127
                  99      99      46      32      32
 
MspA1I CmG'CkG

Cuts at:    162     462     782    1113    1319    1411    2915    3208    3258 
   Size:        300     320     331     206      92    1504     293      50

Cuts at:   3258    5462    5776    6136    7151    7396     162 
   Size:       2204     314     360    1015     245    1119

  Fragments arranged by size: 

                2204    1504    1119    1015     360     331     320     314
                 300     293     245     206      92      50
 
NaeI GCC'GGC

Cuts at:   6532    6532 
   Size:       8353
 
NarI GG'CG_CC

Cuts at:   5782    6030    5782 
   Size:        248    8105
 
NcoI C'CATG_G

Cuts at:   1145    3137    3197    3665    4611    1145 
   Size:       1992      60     468     946    4887

  Fragments arranged by size: 

                4887    1992     946     468      60
 
NdeI CA'TA_TG

Cuts at:   1986    3687    1986 
   Size:       1701    6652
 
NgoMIV G'CCGG_C

Cuts at:   6530    6530 
   Size:       8353
 
NheI G'CTAG_C

Cuts at:   2211    2211 
   Size:       8353
 
NruI TCG'CGA

Cuts at:   6714    6714 
   Size:       8353
 
NsiI A_TGCA'T

Cuts at:    629    1881    3441     629 
   Size:       1252    1560    5541

  Fragments arranged by size: 

                5541    1560    1252
 
NspI r_CATG'y

Cuts at:    631    3478    4266    4335    5404    5904    6813     631 
   Size:       2847     788      69    1069     500     909    2171

  Fragments arranged by size: 

                2847    2171    1069     909     788     500      69
 
PasI CC'CwG_GG

Cuts at:   4773    4931    4773 
   Size:        158    8195
 
PciI A'CATG_T

Cuts at:   6809    6809 
   Size:       8353
 
PflMI CCAn_nnn'nTGG

Cuts at:    402    1441    2007    5067     402 
   Size:       1039     566    3060    3688

  Fragments arranged by size: 

                3688    3060    1039     566
 
PfoI T'CCnGG_A

Cuts at:    428    1614     428 
   Size:       1186    7167
 
PmeI GTTT'AAAC

Cuts at:   5350    5350 
   Size:       8353
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    888     920    7492    7524     888 
   Size:         32    6572      32    1717

  Fragments arranged by size: 

                6572    1717      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    958    2267    2556    2828    3252    5076    5255     958 
   Size:       1309     289     272     424    1824     179    4056

  Fragments arranged by size: 

                4056    1824    1309     424     289     272     179
 
PshAI GACnn'nnGTC

Cuts at:    588     588 
   Size:       8353
 
PsiI TTA'TAA

Cuts at:   3680    3680 
   Size:       8353
 
PspOMI G'GGCC_C

Cuts at:   4073    4759    4073 
   Size:        686    7667
 
PspXI vC'TCGA_Gb

Cuts at:   3131    3131 
   Size:       8353
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1044    1076    3092    3124    1044 
   Size:         32    2016      32    6273

  Fragments arranged by size: 

                6273    2016      32      32
 
PstI C_TGCA'G

Cuts at:   1112    2349    3024    4696    5398    6083    1112 
   Size:       1237     675    1672     702     685    3382

  Fragments arranged by size: 

                3382    1672    1237     702     685     675
 
PvuI CG_AT'CG

Cuts at:    104    2382     104 
   Size:       2278    6075
 
PvuII CAG'CTG

Cuts at:    462    1411    2915    3208    5776    6136     462 
   Size:        949    1504     293    2568     360    2679

  Fragments arranged by size: 

                2679    2568    1504     949     360     293
 
RsrII CG'GwC_CG

Cuts at:   6546    6546 
   Size:       8353
 
SacI G_AGCT'C

Cuts at:   3766    5365    3766 
   Size:       1599    6754
 
SalI G'TCGA_C

Cuts at:   5388    5388 
   Size:       8353
 
SanDI GG'GwC_CC

Cuts at:   2556    2828    3252    5076    2556 
   Size:        272     424    1824    5833

  Fragments arranged by size: 

                5833    1824     424     272
 
SapI GCTCTTCn'nnn_

Cuts at:   1249    6374    6584    1249 
   Size:       5125     210    3018

  Fragments arranged by size: 

                5125    3018     210
 
SbfI CC_TGCA'GG

Cuts at:   5398    5398 
   Size:       8353
 
ScaI AGT'ACT

Cuts at:   7870    7870 
   Size:       8353
 
SexAI A'CCwGG_T

Cuts at:   2012    2012 
   Size:       8353
 
SfcI C'TryA_G

Cuts at:     33     558    1108    2084    2345    2700    3020    3203    4020 
   Size:        525     550     976     261     355     320     183     817

Cuts at:   4020    4560    4692    5264    5394    6079    7074    7265      33 
   Size:        540     132     572     130     685     995     191    1121

  Fragments arranged by size: 

                1121     995     976     817     685     572     550     540
                 525     355     320     261     191     183     132     130
            
 
SfoI GGC'GCC

Cuts at:   5783    6031    5783 
   Size:        248    8105
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       8353
 
SmaI CCC'GGG

Cuts at:   3250    4213    5373    3250 
   Size:        963    1160    6230

  Fragments arranged by size: 

                6230    1160     963
 
SmlI C'TyrA_G

Cuts at:   1425    1686    2064    3131    4309    5506    6421    6915    7177 
   Size:        261     378    1067    1178    1197     915     494     262

Cuts at:   7177    1425 
   Size:       2601

  Fragments arranged by size: 

                2601    1197    1178    1067     915     494     378     262
                 261
 
SnaBI TAC'GTA

Cuts at:   5343    5343 
   Size:       8353
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       8353
 
SphI G_CATG'C

Cuts at:    631    3478    4266    4335    5404    5904     631 
   Size:       2847     788      69    1069     500    3080

  Fragments arranged by size: 

                3080    2847    1069     788     500      69
 
StuI AGG'CCT

Cuts at:   1020    2365    3221    1020 
   Size:       1345     856    6152

  Fragments arranged by size: 

                6152    1345     856
 
StyI C'CwwG_G

Cuts at:   1145    1553    2903    3137    3197    3665    4159    4526    4611 
   Size:        408    1350     234      60     468     494     367      85

Cuts at:   4611    5485    5839    1145 
   Size:        874     354    3659

  Fragments arranged by size: 

                3659    1350     874     494     468     408     367     354
                 234      85      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1954    4420    5934    6067    6526    6737    1954 
   Size:       2466    1514     133     459     211    3570

  Fragments arranged by size: 

                3570    2466    1514     459     211     133
 
TatI w'GTAC_w

Cuts at:    422    4181    7868     422 
   Size:       3759    3687     907

  Fragments arranged by size: 

                3759    3687     907
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    557     589    1107    1139    3443    3475    7870    7902     557 
   Size:         32     518      32    2304      32    4395      32    1008

  Fragments arranged by size: 

                4395    2304    1008     518      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   6148    6148 
   Size:       8353
 
XbaI T'CTAG_A

Cuts at:   2355    5382    2355 
   Size:       3027    5326
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    840    1225    2919    3330    4792    5846     840 
   Size:        385    1694     411    1462    1054    3347

  Fragments arranged by size: 

                3347    1694    1462    1054     411     385
 
XhoI C'TCGA_G

Cuts at:   3131    3131 
   Size:       8353
 
XmaI C'CCGG_G

Cuts at:   3248    4211    5371    3248 
   Size:        963    1160    6230

  Fragments arranged by size: 

                6230    1160     963
 
XmnI GAAnn'nnTTC

Cuts at:   2577    3797    2577 
   Size:       1220    7133
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AscI     BglI    BsiWI    BsmBI   BstEII
     ClaI    CspCI    EcoRV     FseI    FspAI     HpaI     NotI     PacI
     PmlI    SacII     SfiI    SgrAI     SrfI     SspI     SwaI     ZraI
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