Restriction map for pF1KSDA0712
 (Linear) (Six-base) MAPSORT of: KIAA0712.seq  Check: 9963  from: 1  to: 5214



KIAA0712.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3149    5214 
   Size:       3149    2065
 
AccI GT'mk_AC

Cuts at:      0    2158    2387    5214 
   Size:       2158     229    2827

  Fragments arranged by size: 

                2827    2158     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     555     829     989    1510    1592    1889    1919 
   Size:         49     506     274     160     521      82     297      30

Cuts at:   1919    2213    2438    2522    2824    3847    5214 
   Size:        294     225      84     302    1023    1367

  Fragments arranged by size: 

                1367    1023     521     506     302     297     294     274
                 225     160      84      82      49      30
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3024    5214 
   Size:       3024    2190
 
AleI CACnn'nnGTG

Cuts at:      0    3301    3562    5214 
   Size:       3301     261    1652

  Fragments arranged by size: 

                3301    1652     261
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     691    4838    5214 
   Size:        691    4147     376

  Fragments arranged by size: 

                4147     691     376
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2847    2879    5214 
   Size:       2847      32    2335

  Fragments arranged by size: 

                2847    2335      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27    1881    2332    4345    4847    5214 
   Size:         27    1854     451    2013     502     367

  Fragments arranged by size: 

                2013    1854     502     451     367      27
 
ApaI G_GGCC'C

Cuts at:      0    3951    4637    5214 
   Size:       3951     686     577

  Fragments arranged by size: 

                3951     686     577
 
ApoI r'AATT_y

Cuts at:      0      90     214     346     853    2638    5214 
   Size:         90     124     132     507    1785    2576

  Fragments arranged by size: 

                2576    1785     507     132     124      90
 
AvaI C'yCGr_G

Cuts at:      0    3005    3122    4085    4414    4447    5214 
   Size:       3005     117     963     329      33     767

  Fragments arranged by size: 

                3005     963     767     329     117      33
 
AvrII C'CTAG_G

Cuts at:      0    4033    5214 
   Size:       4033    1181
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3787    3820    5214 
   Size:       3787      33    1394

  Fragments arranged by size: 

                3787    1394      33
 
BamHI G'GATC_C

Cuts at:      0    2337    5214 
   Size:       2337    2877
 
BanI G'GyrC_C

Cuts at:      0    3118    3514    5214 
   Size:       3118     396    1700

  Fragments arranged by size: 

                3118    1700     396
 
BanII G_rGCy'C

Cuts at:      0     531    1306    1567    3640    3951    4470    4637    5214 
   Size:        531     775     261    2073     311     519     167     577

  Fragments arranged by size: 

                2073     775     577     531     519     311     261     167
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1137    2510    3538    3620    3674    3716    4564    5214 
   Size:       1137    1373    1028      82      54      42     848     650

  Fragments arranged by size: 

                1373    1137    1028     848     650      82      54      42
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1286    5051    5214 
   Size:       1286    3765     163

  Fragments arranged by size: 

                3765    1286     163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3597    3631    5214 
   Size:       3597      34    1583

  Fragments arranged by size: 

                3597    1583      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     732    1414    5214 
   Size:        732     682    3800

  Fragments arranged by size: 

                3800     732     682
 
BclI T'GATC_A

Cuts at:      0    2484    5214 
   Size:       2484    2730
 
BfrBI ATG'CAT

Cuts at:      0     501    1753    3313    5214 
   Size:        501    1252    1560    1901

  Fragments arranged by size: 

                1901    1560    1252     501
 
BglII A'GATC_T

Cuts at:      0    1138    2890    5214 
   Size:       1138    1752    2324

  Fragments arranged by size: 

                2324    1752    1138
 
Bme1580I G_kGCm'C

Cuts at:      0    3123    3951    4590    4637    5102    5214 
   Size:       3123     828     639      47     465     112

  Fragments arranged by size: 

                3123     828     639     465     112      47
 
BmgBI CAC'GTC

Cuts at:      0     231    4081    5214 
   Size:        231    3850    1133

  Fragments arranged by size: 

                3850    1133     231
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2342    4949    5010    5214 
   Size:       2342    2607      61     204

  Fragments arranged by size: 

                2607    2342     204      61
 
BmtI G_CTAG'C

Cuts at:      0    2089    5214 
   Size:       2089    3125
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3491    4250    4854    5214 
   Size:       3491     759     604     360

  Fragments arranged by size: 

                3491     759     604     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463    1408    1472    2263    2738    3222    4742    4824 
   Size:        463     945      64     791     475     484    1520      82

Cuts at:   4824    5214 
   Size:        390

  Fragments arranged by size: 

                1520     945     791     484     475     463     390      82
                  64
 
Bpu10I CC'TnA_GC

Cuts at:      0     779    1286    3344    4577    5051    5214 
   Size:        779     507    2058    1233     474     163

  Fragments arranged by size: 

                2058    1233     779     507     474     163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1320    1581    1923    4204    5214 
   Size:       1320     261     342    2281    1010

  Fragments arranged by size: 

                2281    1320    1010     342     261
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2127    3981    5214 
   Size:       2127    1854    1233

  Fragments arranged by size: 

                2127    1854    1233
 
BsaBI GATnn'nnATC

Cuts at:      0     394    1337    5214 
   Size:        394     943    3877

  Fragments arranged by size: 

                3877     943     394
 
BsaWI w'CCGG_w

Cuts at:      0    3692    4217    5214 
   Size:       3692     525     997

  Fragments arranged by size: 

                3692     997     525
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2736    2766    5214 
   Size:       2736      30    2448

  Fragments arranged by size: 

                2736    2448      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1087    1249    1265    1511    3363    3630    4554    4920 
   Size:       1087     162      16     246    1852     267     924     366

Cuts at:   4920    5078    5214 
   Size:        158     136

  Fragments arranged by size: 

                1852    1087     924     366     267     246     162     158
                 136      16
 
BseYI C'CCAG_C

Cuts at:      0    1182    1260    2152    2789    5214 
   Size:       1182      78     892     637    2425

  Fragments arranged by size: 

                2425    1182     892     637      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     824    3121    3167    3266    3721    4312    5214 
   Size:        111     713    2297      46      99     455     591     902

  Fragments arranged by size: 

                2297     902     713     591     455     111      99      46
            
 
BsiEI CG_ry'CG

Cuts at:      0    2256    5214 
   Size:       2256    2958
 
BsiHKAI G_wGCw'C

Cuts at:      0      83     230    2776    3640    4236    4583    5214 
   Size:         83     147    2546     864     596     347     631

  Fragments arranged by size: 

                2546     864     631     596     347     147      83
 
BsmI GAATG_Cn'

Cuts at:      0     133    1755    5214 
   Size:        133    1622    3459

  Fragments arranged by size: 

                3459    1622     133
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     230     531    1306    1567    2776    3123    3640 
   Size:         83     147     301     775     261    1209     347     517

Cuts at:   3640    3951    4236    4470    4583    4590    4637    5102    5214 
   Size:        311     285     234     113       7      47     465     112

  Fragments arranged by size: 

                1209     775     517     465     347     311     301     285
                 261     234     147     113     112      83      47       7
            
 
BspEI T'CCGG_A

Cuts at:      0    3692    4217    5214 
   Size:       3692     525     997

  Fragments arranged by size: 

                3692     997     525
 
BspHI T'CATG_A

Cuts at:      0    1218    5214 
   Size:       1218    3996
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     102     843    2910    3149    5214 
   Size:        102     741    2067     239    2065

  Fragments arranged by size: 

                2067    2065     741     239     102
 
BsrBI CCG'CTC

Cuts at:      0    3839    5214 
   Size:       3839    1375
 
BsrDI GCAATG_nn'

Cuts at:      0    3313    5214 
   Size:       3313    1901
 
BsrGI T'GTAC_A

Cuts at:      0    4055    5214 
   Size:       4055    1159
 
BssHII G'CGCG_C

Cuts at:      0    3144    5214 
   Size:       3144    2070
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1159    5214 
   Size:       1159    4055
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3207    3303    3511    4654    4720    4794    5214 
   Size:       3207      96     208    1143      66      74     420

  Fragments arranged by size: 

                3207    1143     420     208      96      74      66
 
BstYI r'GATC_y

Cuts at:      0    1138    1804    2337    2890    4179    4310    4341    5201 
   Size:       1138     666     533     553    1289     131      31     860

Cuts at:   5201    5214 
   Size:         13

  Fragments arranged by size: 

                1289    1138     860     666     553     533     131      31
                  13
 
BstZ17I GTA'TAC

Cuts at:      0    2159    5214 
   Size:       2159    3055
 
Bsu36I CC'TnA_GG

Cuts at:      0    1367    2006    4537    5214 
   Size:       1367     639    2531     677

  Fragments arranged by size: 

                2531    1367     677     639
 
BtgI C'CryG_G

Cuts at:      0    1019    1702    3011    3071    3539    4485    5214 
   Size:       1019     683    1309      60     468     946     729

  Fragments arranged by size: 

                1309    1019     946     729     683     468      60
 
BtsI GCAGTG_nn'

Cuts at:      0    2813    3187    3249    3284    5004    5214 
   Size:       2813     374      62      35    1720     210

  Fragments arranged by size: 

                2813    1720     374     210      62      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    3507    5214 
   Size:       3507    1707
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    5010    5214 
   Size:       5010     204
 
EaeI y'GGCC_r

Cuts at:      0     671    3882    4656    5214 
   Size:        671    3211     774     558

  Fragments arranged by size: 

                3211     774     671     558
 
EarI CTCTTCn'nnn_

Cuts at:      0     473     749    1123    1529    2125    2705    5214 
   Size:        473     276     374     406     596     580    2509

  Fragments arranged by size: 

                2509     596     580     473     406     374     276
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2867    3707    5214 
   Size:       2867     840    1507

  Fragments arranged by size: 

                2867    1507     840
 
EcoICRI GAG'CTC

Cuts at:      0    3638    5214 
   Size:       3638    1576
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     463     555     829     989    1408    1472    1510 
   Size:         49     414      92     274     160     419      64      38

Cuts at:   1510    1592    1889    1919    2213    2263    2438    2522    2738 
   Size:         82     297      30     294      50     175      84     216

Cuts at:   2738    2824    3222    3847    4742    4824    5214 
   Size:         86     398     625     895      82     390

  Fragments arranged by size: 

                 895     625     419     414     398     390     297     294
                 274     216     175     160      92      86      84      82
                  82      64      50      49      38      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1885    3381    3645    4809    5214 
   Size:       1885    1496     264    1164     405

  Fragments arranged by size: 

                1885    1496    1164     405     264
 
EcoO109I rG'GnC_Cy

Cuts at:      0     832    2141    2430    2702    3126    3947    3948    4252 
   Size:        832    1309     289     272     424     821       1     304

Cuts at:   4252    4634    4950    5129    5156    5214 
   Size:        382     316     179      27      58

  Fragments arranged by size: 

                1309     832     821     424     382     316     304     289
                 272     179      58      27       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     315    5214 
   Size:        315    4899
 
FspI TGC'GCA

Cuts at:      0    3164    5214 
   Size:       3164    2050
 
HaeII r_GCGC'y

Cuts at:      0    2086    4992    5214 
   Size:       2086    2906     222

  Fragments arranged by size: 

                2906    2086     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     137     169    1141    1173    1330    1362    1567    1599 
   Size:        137      32     972      32     157      32     205      32

Cuts at:   1599    1915    1947    2188    2220    2736    2768    3010    3042 
   Size:        316      32     241      32     516      32     242      32

Cuts at:   3042    3610    3642    4495    4527    4657    4689    5026    5058 
   Size:        568      32     853      32     130      32     337      32

Cuts at:   5058    5214 
   Size:        156

  Fragments arranged by size: 

                 972     853     568     516     337     316     242     241
                 205     157     156     137     130      32      32      32
                  32      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1296    5214 
   Size:       1296    3918
 
HindIII A'AGCT_T

Cuts at:      0    4549    5214 
   Size:       4549     665
 
MfeI C'AATT_G

Cuts at:      0     518     794    5214 
   Size:        518     276    4420

  Fragments arranged by size: 

                4420     518     276
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1146    2375    5214 
   Size:         37    1109    1229    2839

  Fragments arranged by size: 

                2839    1229    1109      37
 
MscI TGG'CCA

Cuts at:      0     673    4658    5214 
   Size:        673    3985     556

  Fragments arranged by size: 

                3985     673     556
 
MslI CAynn'nnrTG

Cuts at:      0     496     595     641     673    1223    1353    2179    3170 
   Size:        496      99      46      32     550     130     826     991

Cuts at:   3170    3202    3301    3562    5214 
   Size:         32      99     261    1652

  Fragments arranged by size: 

                1652     991     826     550     496     261     130      99
                  99      46      32      32
 
MspA1I CmG'CkG

Cuts at:      0      36     336     656     987    1193    1285    2789    3082 
   Size:         36     300     320     331     206      92    1504     293

Cuts at:   3082    3132    5214 
   Size:         50    2082

  Fragments arranged by size: 

                2082    1504     331     320     300     293     206      92
                  50      36
 
NcoI C'CATG_G

Cuts at:      0    1019    3011    3071    3539    4485    5214 
   Size:       1019    1992      60     468     946     729

  Fragments arranged by size: 

                1992    1019     946     729     468      60
 
NdeI CA'TA_TG

Cuts at:      0    1860    3561    5214 
   Size:       1860    1701    1653

  Fragments arranged by size: 

                1860    1701    1653
 
NheI G'CTAG_C

Cuts at:      0    2085    5214 
   Size:       2085    3129
 
NsiI A_TGCA'T

Cuts at:      0     503    1755    3315    5214 
   Size:        503    1252    1560    1899

  Fragments arranged by size: 

                1899    1560    1252     503
 
NspI r_CATG'y

Cuts at:      0     505    3352    4140    4209    5214 
   Size:        505    2847     788      69    1005

  Fragments arranged by size: 

                2847    1005     788     505      69
 
PasI CC'CwG_GG

Cuts at:      0    4647    4805    5214 
   Size:       4647     158     409

  Fragments arranged by size: 

                4647     409     158
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     276    1315    1881    4941    5214 
   Size:        276    1039     566    3060     273

  Fragments arranged by size: 

                3060    1039     566     276     273
 
PfoI T'CCnGG_A

Cuts at:      0     302    1488    5214 
   Size:        302    1186    3726

  Fragments arranged by size: 

                3726    1186     302
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     762     794    5214 
   Size:        762      32    4420

  Fragments arranged by size: 

                4420     762      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     832    2141    2430    2702    3126    4950    5129    5214 
   Size:        832    1309     289     272     424    1824     179      85

  Fragments arranged by size: 

                1824    1309     832     424     289     272     179      85
            
 
PshAI GACnn'nnGTC

Cuts at:      0     462    5214 
   Size:        462    4752
 
PsiI TTA'TAA

Cuts at:      0    3554    5214 
   Size:       3554    1660
 
PspOMI G'GGCC_C

Cuts at:      0    3947    4633    5214 
   Size:       3947     686     581

  Fragments arranged by size: 

                3947     686     581
 
PspXI vC'TCGA_Gb

Cuts at:      0    3005    5214 
   Size:       3005    2209
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     918     950    2966    2998    5214 
   Size:        918      32    2016      32    2216

  Fragments arranged by size: 

                2216    2016     918      32      32
 
PstI C_TGCA'G

Cuts at:      0     986    2223    2898    4570    5214 
   Size:        986    1237     675    1672     644

  Fragments arranged by size: 

                1672    1237     986     675     644
 
PvuI CG_AT'CG

Cuts at:      0    2256    5214 
   Size:       2256    2958
 
PvuII CAG'CTG

Cuts at:      0     336    1285    2789    3082    5214 
   Size:        336     949    1504     293    2132

  Fragments arranged by size: 

                2132    1504     949     336     293
 
SacI G_AGCT'C

Cuts at:      0    3640    5214 
   Size:       3640    1574
 
SanDI GG'GwC_CC

Cuts at:      0    2430    2702    3126    4950    5214 
   Size:       2430     272     424    1824     264

  Fragments arranged by size: 

                2430    1824     424     272     264
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1123    5214 
   Size:       1123    4091
 
SexAI A'CCwGG_T

Cuts at:      0    1886    5214 
   Size:       1886    3328
 
SfcI C'TryA_G

Cuts at:      0     432     982    1958    2219    2574    2894    3077    3894 
   Size:        432     550     976     261     355     320     183     817

Cuts at:   3894    4434    4566    5138    5214 
   Size:        540     132     572      76

  Fragments arranged by size: 

                 976     817     572     550     540     432     355     320
                 261     183     132      76
 
SmaI CCC'GGG

Cuts at:      0    3124    4087    5214 
   Size:       3124     963    1127

  Fragments arranged by size: 

                3124    1127     963
 
SmlI C'TyrA_G

Cuts at:      0    1299    1560    1938    3005    4183    5214 
   Size:       1299     261     378    1067    1178    1031

  Fragments arranged by size: 

                1299    1178    1067    1031     378     261
 
SphI G_CATG'C

Cuts at:      0     505    3352    4140    4209    5214 
   Size:        505    2847     788      69    1005

  Fragments arranged by size: 

                2847    1005     788     505      69
 
StuI AGG'CCT

Cuts at:      0     894    2239    3095    5214 
   Size:        894    1345     856    2119

  Fragments arranged by size: 

                2119    1345     894     856
 
StyI C'CwwG_G

Cuts at:      0    1019    1427    2777    3011    3071    3539    4033    4400 
   Size:       1019     408    1350     234      60     468     494     367

Cuts at:   4400    4485    5214 
   Size:         85     729

  Fragments arranged by size: 

                1350    1019     729     494     468     408     367     234
                  85      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1828    4294    5214 
   Size:       1828    2466     920

  Fragments arranged by size: 

                2466    1828     920
 
TatI w'GTAC_w

Cuts at:      0     296    4055    5214 
   Size:        296    3759    1159

  Fragments arranged by size: 

                3759    1159     296
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     431     463     981    1013    3317    3349    5214 
   Size:        431      32     518      32    2304      32    1865

  Fragments arranged by size: 

                2304    1865     518     431      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2229    5214 
   Size:       2229    2985
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     714    1099    2793    3204    4666    5214 
   Size:        714     385    1694     411    1462     548

  Fragments arranged by size: 

                1694    1462     714     548     411     385
 
XhoI C'TCGA_G

Cuts at:      0    3005    5214 
   Size:       3005    2209
 
XmaI C'CCGG_G

Cuts at:      0    3122    4085    5214 
   Size:       3122     963    1129

  Fragments arranged by size: 

                3122    1129     963
 
XmnI GAAnn'nnTTC

Cuts at:      0    2451    3671    5214 
   Size:       2451    1220    1543

  Fragments arranged by size: 

                2451    1543    1220
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI     AlfI     AloI    AlwNI
     ApaI     ApoI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    HaeII    Hin4I   HincII  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NsiI
     NspI     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII     SacI    SanDI
     SapI    SexAI     SfcI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI    ApaLI
     AscI     AseI    AsiSI     BbeI     BglI     BlpI    BsaAI    BsaHI
    BsiWI    BsmBI    BsrFI    BssSI    BstBI   BstEII    BtgZI     ClaI
    CspCI     DraI     EagI    EcoRI    EcoRV     FseI    FspAI     HpaI
     KasI     KpnI     MluI     NaeI     NarI   NgoMIV     NotI     NruI
     PacI     PciI     PmeI     PmlI    RsrII    SacII     SalI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com