Restriction map for pF1KSDA0694
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EcoNI       EcoO65I     EcoRI       FseI        FspI        
Hin1I       HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        RsrII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tsp45I      
Tth111I     Van91I      XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AclI         1754  AACGTT               AA^CGTT
AlwNI         284  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          877  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       623  AGCGCT               AGC^GCT
ApaI          883  GGGCCC               GGGCC^C
AvaII          14  GGACC                G^G[AT]CC
AvrII         133  CCTAGG               C^CTAGG
BalI         1523  TGGCCA               TGG^CCA
BamHI         842  GGATCC               G^GATCC
BanI         1564  GGTGCC               G^G[CT][GA]CC
BbsI          507  GTCTTC               GAAGAC(2/6)
BcnI           29  CCCGG                CC^[GC]GG
BglI           88  GCCGCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          133  CCTAGG               C^CTAGG
BpmI          392  CTGGAG               CTGGAG(16/14)
BpuAI         507  GTCTTC               GAAGAC(2/6)
BsaI           42  GAGACC               GGTCTC(1/5)
BsaWI         191  ACCGGA               [AT]^CCGG[AT]
BsgI          742  GTGCAG               GTGCAG(16/14)
BsiEI          56  CGGCCG               CG[GA][CT]^CG
BsmFI        1443  GGGAC                GGGAC(10/14)
Bsp1407I      730  TGTACA               T^GTACA
BspHI        1181  TCATGA               T^CATGA
BspMI          20  GCAGGT               ACCTGC(4/8)
BsrDI        1554  CATTGC               GCAATG(2/0)
BsrGI         730  TGTACA               T^GTACA
BstXI        1450  CCAGAGGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        877  CCTGAGG              CC^T[ACGT]AGG
DsaI           89  CCGCGG               C^C[GA][CT]GG
EagI           56  CGGCCG               C^GGCCG
EarI          203  CTCTTC               CTCTTC(1/4)
EclXI          56  CGGCCG               C^GGCCG
Eco47III      623  AGCGCT               AGC^GCT
Eco52I         56  CGGCCG               C^GGCCG
Eco81I        877  CCTGAGG              CC^T[ACGT]AGG
EcoRV         262  GATATC               GAT^ATC
EcoT22I       711  ATGCAT               ATGCA^T
HgaI           79  GCGTC                GACGC(5/10)
HincII       1690  GTTGAC               GT[CT]^[GA]AC
HindII       1690  GTTGAC               GT[CT]^[GA]AC
Ksp632I       203  CTCTTC               CTCTTC(1/4)
KspI           89  CCGCGG               CCGC^GG
MluNI        1523  TGGCCA               TGG^CCA
MscI         1523  TGGCCA               TGG^CCA
MslI          466  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          107  GCCGGC               GCC^GGC
NciI           29  CCCGG                CC^[GC]GG
NgoMIV        107  GCCGGC               G^CCGGC
NsiI          711  ATGCAT               ATGCA^T
NspI          360  GCATGT               [GA]CATG^[CT]
PaeR7I        142  CTCGAG               C^TCGAG
Psp1406I     1754  AACGTT               AA^CGTT
PstI         1638  CTGCAG               CTGCA^G
RcaI         1181  TCATGA               T^CATGA
SacII          89  CCGCGG               CCGC^GG
SfcI         1638  CTGCAG               C^T[GA][CT]AG
SmlI          142  CTCGAG               C^T[CT][GA]AG
SspBI         730  TGTACA               T^GTACA
XbaI         1083  TCTAGA               T^CTAGA
XhoI          142  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          78  CGCG                 CG^CG
               90  CGCG                
AvaI          142  CTCGAG               C^[CT]CG[GA]G
              413  CCCGAG              
BclI         1145  TGATCA               T^GATCA
             1184  TGATCA              
BglII         210  AGATCT               A^GATCT
             1122  AGATCT              
BsmI          958  GAATGC               GAATGC(1/-1)
              860  GCATTC              
BsoBI         142  CTCGAG               C^[CT]CG[GA]G
              413  CCCGAG              
BsrBI          10  GAGCGG               CCGCTC(-3/-3)
              527  GAGCGG              
BsrFI           4  GCCGGT               [GA]^CCGG[CT]
              107  GCCGGC              
BstAPI       1003  GCAGACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1558  GCAAGTGGTGC         
BstNI          46  CCTGG                CC^[AT]GG
              257  CCTGG               
BstUI          78  CGCG                 CG^CG
               90  CGCG                
CfoI           74  GCGC                 GCG^C
              624  GCGC                
Cfr10I          4  GCCGGT               [GA]^CCGG[CT]
              107  GCCGGC              
DraII         882  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              883  GGGCCCC             
EcoO109I      882  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              883  GGGCCCC             
EcoRII         46  CCTGG                ^CC[AT]GG
              257  CCTGG               
FauI           17  CCCGC                CCCGC(4/6)
              529  GCGGG               
FbaI         1145  TGATCA               T^GATCA
             1184  TGATCA              
HaeII          73  AGCGCC               [GA]GCGC^[CT]
              623  AGCGCT              
HhaI           74  GCGC                 GCG^C
              624  GCGC                
HinP1I         74  GCGC                 G^CGC
              624  GCGC                
HindIII       436  AAGCTT               A^AGCTT
             1740  AAGCTT              
HphI            7  GGTGA                GGTGA(8/7)
               26  TCACC               
MaeII         271  ACGT                 A^CGT
             1755  ACGT                
MvaI           46  CCTGG                CC^[AT]GG
              257  CCTGG               
MvnI           78  CGCG                 CG^CG
               90  CGCG                
PvuII         634  CAGCTG               CAG^CTG
              812  CAGCTG              
SacI           61  GAGCTC               GAGCT^C
              910  GAGCTC              
SspI          386  AATATT               AAT^ATT
             1255  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       BanII       BbvI        BfaI        
BmyI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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