Restriction map for pF1KSDA0659
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BamHI       BfaI        BfrI        BglII       
BlnI        BsiWI       BsmBI       BspDI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        DrdI        Eco47III    
EcoRV       FspI        HincII      HindII      HindIII     
HpaI        KpnI        MaeI        MfeI        MluI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        TfiI        Tru9I       XbaI        
XcmI        XhoI        XmnI        


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# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI         2280  GTCTAC               GT^[AC][GT]AC
AccIII       3056  TCCGGA               T^CCGGA
ApaI         2844  GGGCCC               GGGCC^C
BbrPI        1819  CACGTG               CAC^GTG
BbvCI        2839  GCTGAGG              CCTCAGC(-5/-2)
BsaI         1364  GAGACC               GGTCTC(1/5)
BsaWI        3056  TCCGGA               [AT]^CCGG[AT]
BseAI        3056  TCCGGA               T^CCGGA
BsmAI        1364  GAGAC                GTCTC(1/5)
BsmI         3034  GAATGC               GAATGC(1/-1)
Bsp1407I     2366  TGTACA               T^GTACA
BspEI        3056  TCCGGA               T^CCGGA
BsrGI        2366  TGTACA               T^GTACA
BstAPI        282  GCAGTACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       2428  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1344  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1683  ATGCAT               ATGCA^T
FseI         2663  GGCCGGCC             GGCCGG^CC
MroI         3056  TCCGGA               T^CCGGA
NcoI         2336  CCATGG               C^CATGG
NsiI         1683  ATGCAT               ATGCA^T
PmaCI        1819  CACGTG               CAC^GTG
PmlI         1819  CACGTG               CAC^GTG
SapI          117  GCTCTTC              GCTCTTC(1/4)
ScaI          362  AGTACT               AGT^ACT
SmaI            4  CCCGGG               CCC^GGG
SphI          239  GCATGC               GCATG^C
SspBI        2366  TGTACA               T^GTACA
XmaCI           4  CCCGGG               C^CCGGG
XmaI            4  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          843  GAATTC               [GA]^AATT[CT]
             2734  GAATTC              
ApoI          843  GAATTC               [GA]^AATT[CT]
             2734  GAATTC              
BbeI         1064  GGCGCC               GGCGC^C
             2699  GGCGCC              
BciVI        1187  GTATCC               GTATCC(6/5)
             1464  GTATCC              
BclI         1181  TGATCA               T^GATCA
             1919  TGATCA              
BsaBI         219  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1182  GATCAGTATC          
BsgI          609  CTGCAC               GTGCAG(16/14)
             2689  CTGCAC              
BssSI         142  CACGAG               CACGAG(-5/-1)
              972  CTCGTG              
BstEII        810  GGTGACC              G^GT[ACGT]ACC
             1719  GGTGACC             
BstPI         810  GGTGACC              G^GT[ACGT]ACC
             1719  GGTGACC             
BstXI        1350  CCAGGCCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2030  CCAAGGTCATGG        
BstYI        2870  AGATCC               [GA]^GATC[CT]
             3053  AGATCC              
EcoO65I       810  GGTGACC              G^GT[ACGT]ACC
             1719  GGTGACC             
EcoRI         843  GAATTC               G^AATTC
             2734  GAATTC              
FbaI         1181  TGATCA               T^GATCA
             1919  TGATCA              
KasI         1064  GGCGCC               G^GCGCC
             2699  GGCGCC              
MamI          219  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1182  GATCAGTATC          
MflI         2870  AGATCC               [GA]^GATC[CT]
             3053  AGATCC              
NarI         1064  GGCGCC               GG^CGCC
             2699  GGCGCC              
NspI          239  GCATGC               [GA]CATG^[CT]
              884  ACATGC              
SfoI         1064  GGCGCC               GGC^GCC
             2699  GGCGCC              
SmlI          838  CTCAAG               C^T[CT][GA]AG
             1474  CTCAAG              
Tsp509I       844  AATT                 ^AATT
             2735  AATT                
XhoII        2870  AGATCC               [GA]^GATC[CT]
             3053  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       AocI        AspEI       
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco52I      Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      
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