Restriction map for pF1KSDA0659
 (Linear) (Six-base) MAPSORT of: KIAA0659.seq  Check: 2909  from: 1  to: 3126



KIAA0659.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1958    3126 
   Size:       1958    1168
 
AccI GT'mk_AC

Cuts at:      0    2281    3126 
   Size:       2281     845
 
AhdI GACnn_n'nnGTC

Cuts at:      0     630    2358    2535    3126 
   Size:        630    1728     177     591

  Fragments arranged by size: 

                1728     630     591     177
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     775    1863    2533    2827    3126 
   Size:        775    1088     670     294     299

  Fragments arranged by size: 

                1088     775     670     299     294
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1623    2565    2730    3126 
   Size:       1623     942     165     396

  Fragments arranged by size: 

                1623     942     396     165
 
ApaI G_GGCC'C

Cuts at:      0    2848    3126 
   Size:       2848     278
 
ApoI r'AATT_y

Cuts at:      0     843    2734    3126 
   Size:        843    1891     392

  Fragments arranged by size: 

                1891     843     392
 
AvaI C'yCGr_G

Cuts at:      0       4    2482    2815    2949    2988    3126 
   Size:          4    2478     333     134      39     138

  Fragments arranged by size: 

                2478     333     138     134      39       4
 
BanI G'GyrC_C

Cuts at:      0     548    1064    1262    1270    2322    2699    2811    2904 
   Size:        548     516     198       8    1052     377     112      93

Cuts at:   2904    3126 
   Size:        222

  Fragments arranged by size: 

                1052     548     516     377     222     198     112      93
                   8
 
BanII G_rGCy'C

Cuts at:      0     269    1874    1976    2047    2075    2416    2506    2824 
   Size:        269    1605     102      71      28     341      90     318

Cuts at:   2824    2848    2897    2999    3126 
   Size:         24      49     102     127

  Fragments arranged by size: 

                1605     341     318     269     127     102     102      90
                  71      49      28      24
 
BbeI G_GCGC'C

Cuts at:      0    1068    2703    3126 
   Size:       1068    1635     423

  Fragments arranged by size: 

                1635    1068     423
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     459    1034    2014    3126 
   Size:        459     575     980    1112

  Fragments arranged by size: 

                1112     980     575     459
 
BbvCI CC'TCA_GC

Cuts at:      0    2840    3126 
   Size:       2840     286
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     370     404     691     725    1554    1588    3126 
   Size:        370      34     287      34     829      34    1538

  Fragments arranged by size: 

                1538     829     370     287      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1198    1475    3126 
   Size:       1198     277    1651

  Fragments arranged by size: 

                1651    1198     277
 
BclI T'GATC_A

Cuts at:      0    1181    1919    3126 
   Size:       1181     738    1207

  Fragments arranged by size: 

                1207    1181     738
 
BfrBI ATG'CAT

Cuts at:      0    1685    3126 
   Size:       1685    1441
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1656    2201    2320    2919    3126 
   Size:       1656     545     119     599     207

  Fragments arranged by size: 

                1656     599     545     207     119
 
BlpI GC'TnA_GC

Cuts at:      0     199     235    1241    2934    2980    3126 
   Size:        199      36    1006    1693      46     146

  Fragments arranged by size: 

                1693    1006     199     146      46      36
 
Bme1580I G_kGCm'C

Cuts at:      0    1265    1429    2325    2816    2848    2886    2909    3126 
   Size:       1265     164     896     491      32      38      23     217

  Fragments arranged by size: 

                1265     896     491     217     164      38      32      23
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     435     624    2175    2606    2711    2880    3126 
   Size:        435     189    1551     431     105     169     246

  Fragments arranged by size: 

                1551     435     431     246     189     169     105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168    2223    2364    3126 
   Size:        168    2055     141     762

  Fragments arranged by size: 

                2055     762     168     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1348    2008    2152    2218    2458    2587    3126 
   Size:       1031     317     660     144      66     240     129     539

  Fragments arranged by size: 

                1031     660     539     317     240     144     129      66
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2069    2500    2600    2840    3126 
   Size:       2069     431     100     240     286

  Fragments arranged by size: 

                2069     431     286     240     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     823    1459    3126 
   Size:        823     636    1667

  Fragments arranged by size: 

                1667     823     636
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1358    3126 
   Size:       1358    1768
 
BsaAI yAC'GTr

Cuts at:      0     288     297    1149    1821    3126 
   Size:        288       9     852     672    1305

  Fragments arranged by size: 

                1305     852     672     288       9
 
BsaBI GATnn'nnATC

Cuts at:      0     223    1186    3126 
   Size:        223     963    1940

  Fragments arranged by size: 

                1940     963     223
 
BsaHI Gr'CG_yC

Cuts at:      0    1065    2591    2700    3010    3126 
   Size:       1065    1526     109     310     116

  Fragments arranged by size: 

                1526    1065     310     116     109
 
BsaWI w'CCGG_w

Cuts at:      0    3056    3126 
   Size:       3056      70
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385     507     537    3126 
   Size:        355      30     122      30    2589

  Fragments arranged by size: 

                2589     355     122      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     528     574    1723    1882    2379    2647    2937    2976 
   Size:        528      46    1149     159     497     268     290      39

Cuts at:   2976    3126 
   Size:        150

  Fragments arranged by size: 

                1149     528     497     290     268     159     150      46
                  39
 
BseYI C'CCAG_C

Cuts at:      0    1278    1756    2419    2857    3126 
   Size:       1278     478     663     438     269

  Fragments arranged by size: 

                1278     663     478     438     269
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     594    2674    3126 
   Size:        594    2080     452

  Fragments arranged by size: 

                2080     594     452
 
BsiEI CG_ry'CG

Cuts at:      0     177    1809    2528    3126 
   Size:        177    1632     719     598

  Fragments arranged by size: 

                1632     719     598     177
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     293    1949    2075    2506    2999    3126 
   Size:        119     174    1656     126     431     493     127

  Fragments arranged by size: 

                1656     493     431     174     127     126     119
 
BsmI GAATG_Cn'

Cuts at:      0    3040    3126 
   Size:       3040      86
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     269     293    1265    1429    1874    1949    1976 
   Size:        119     150      24     972     164     445      75      27

Cuts at:   1976    2047    2075    2325    2416    2506    2816    2824    2848 
   Size:         71      28     250      91      90     310       8      24

Cuts at:   2848    2886    2897    2909    2999    3126 
   Size:         38      11      12      90     127

  Fragments arranged by size: 

                 972     445     310     250     164     150     127     119
                  91      90      90      75      71      38      28      27
                  24      24      12      11       8
 
BspEI T'CCGG_A

Cuts at:      0    3056    3126 
   Size:       3056      70
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     536     551    1958    3126 
   Size:        536      15    1407    1168

  Fragments arranged by size: 

                1407    1168     536      15
 
BsrBI CCG'CTC

Cuts at:      0    2014    2292    2387    2604    2979    3126 
   Size:       2014     278      95     217     375     147

  Fragments arranged by size: 

                2014     375     278     217     147      95
 
BsrDI GCAATG_nn'

Cuts at:      0     707    1052    1529    3126 
   Size:        707     345     477    1597

  Fragments arranged by size: 

                1597     707     477     345
 
BsrFI r'CCGG_y

Cuts at:      0     587     807    2664    2929    3126 
   Size:        587     220    1857     265     197

  Fragments arranged by size: 

                1857     587     265     220     197
 
BsrGI T'GTAC_A

Cuts at:      0    2366    3126 
   Size:       2366     760
 
BssSI C'ACGA_G

Cuts at:      0     142     972    3126 
   Size:        142     830    2154

  Fragments arranged by size: 

                2154     830     142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     288    3126 
   Size:        288    2838
 
BstEII G'GTnAC_C

Cuts at:      0     810    1719    3126 
   Size:        810     909    1407

  Fragments arranged by size: 

                1407     909     810
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1357    2037    3126 
   Size:       1357     680    1089

  Fragments arranged by size: 

                1357    1089     680
 
BstYI r'GATC_y

Cuts at:      0    2870    3053    3126 
   Size:       2870     183      73

  Fragments arranged by size: 

                2870     183      73
 
Bsu36I CC'TnA_GG

Cuts at:      0    1706    2391    2723    3126 
   Size:       1706     685     332     403

  Fragments arranged by size: 

                1706     685     403     332
 
BtgI C'CryG_G

Cuts at:      0     110     171     177     476     997    1253    1266    1374 
   Size:        110      61       6     299     521     256      13     108

Cuts at:   1374    2099    2336    2673    2917    3014    3078    3126 
   Size:        725     237     337     244      97      64      48

  Fragments arranged by size: 

                 725     521     337     299     256     244     237     110
                 108      97      64      61      48      13       6
 
BtsI GCAGTG_nn'

Cuts at:      0      48     386    1427    1852    2589    3126 
   Size:         48     338    1041     425     737     537

  Fragments arranged by size: 

                1041     737     537     425     338      48
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      78     113    1020    1055    1863    1898    2106    2141 
   Size:         78      35     907      35     808      35     208      35

Cuts at:   2141    3126 
   Size:        985

  Fragments arranged by size: 

                 985     907     808     208      78      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2433    3126 
   Size:       2433     693
 
EaeI y'GGCC_r

Cuts at:      0     174     221     305    1730    1806    2330    2525    2621 
   Size:        174      47      84    1425      76     524     195      96

Cuts at:   2621    3075    3126 
   Size:        454      51

  Fragments arranged by size: 

                1425     524     454     195     174      96      84      76
                  51      47
 
EagI C'GGCC_G

Cuts at:      0     174    1806    2525    3126 
   Size:        174    1632     719     601

  Fragments arranged by size: 

                1632     719     601     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     124    2464    2633    2764    3126 
   Size:        124    2340     169     131     362

  Fragments arranged by size: 

                2340     362     169     131     124
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1674    2317    3126 
   Size:       1674     643     809

  Fragments arranged by size: 

                1674     809     643
 
EcoICRI GAG'CTC

Cuts at:      0    2073    2504    2997    3126 
   Size:       2073     431     493     129

  Fragments arranged by size: 

                2073     493     431     129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1348    2008    2152    2218    2458    2587    3126 
   Size:       1031     317     660     144      66     240     129     539

  Fragments arranged by size: 

                1031     660     539     317     240     144     129      66
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1348    3126 
   Size:       1348    1778
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681    1196    1503    2256    2844    2845    3126 
   Size:        681     515     307     753     588       1     281

  Fragments arranged by size: 

                 753     681     588     515     307     281       1
 
EcoRI G'AATT_C

Cuts at:      0     843    2734    3126 
   Size:        843    1891     392

  Fragments arranged by size: 

                1891     843     392
 
FseI GG_CCGG'CC

Cuts at:      0    2668    3126 
   Size:       2668     458
 
HaeII r_GCGC'y

Cuts at:      0      30    1068    1248    2689    2703    3126 
   Size:         30    1038     180    1441      14     423

  Fragments arranged by size: 

                1441    1038     423     180      30      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     616     628     648     660    1004    1036    2344    2376 
   Size:        616      12      20      12     344      32    1308      32

Cuts at:   2376    2521    2553    2932    2964    3126 
   Size:        145      32     379      32     162

  Fragments arranged by size: 

                1308     616     379     344     162     145      32      32
                  32      32      20      12      12
 
KasI G'GCGC_C

Cuts at:      0    1064    2699    3126 
   Size:       1064    1635     427

  Fragments arranged by size: 

                1635    1064     427
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     725    1492    1676    3126 
   Size:        725     767     184    1450

  Fragments arranged by size: 

                1450     767     725     184
 
MscI TGG'CCA

Cuts at:      0     223     307    1732    2332    3077    3126 
   Size:        223      84    1425     600     745      49

  Fragments arranged by size: 

                1425     745     600     223      84      49
 
MslI CAynn'nnrTG

Cuts at:      0     313    1819    2809    3126 
   Size:        313    1506     990     317

  Fragments arranged by size: 

                1506     990     317     313
 
MspA1I CmG'CkG

Cuts at:      0     179     578     735     741     999    1306    1376    1599 
   Size:        179     399     157       6     258     307      70     223

Cuts at:   1599    2675    3126 
   Size:       1076     451

  Fragments arranged by size: 

                1076     451     399     307     258     223     179     157
                  70       6
 
NaeI GCC'GGC

Cuts at:      0     589    2666    2931    3126 
   Size:        589    2077     265     195

  Fragments arranged by size: 

                2077     589     265     195
 
NarI GG'CG_CC

Cuts at:      0    1065    2700    3126 
   Size:       1065    1635     426

  Fragments arranged by size: 

                1635    1065     426
 
NcoI C'CATG_G

Cuts at:      0    2336    3126 
   Size:       2336     790
 
NgoMIV G'CCGG_C

Cuts at:      0     587    2664    2929    3126 
   Size:        587    2077     265     197

  Fragments arranged by size: 

                2077     587     265     197
 
NsiI A_TGCA'T

Cuts at:      0    1687    3126 
   Size:       1687    1439
 
NspI r_CATG'y

Cuts at:      0     243     888    3126 
   Size:        243     645    2238

  Fragments arranged by size: 

                2238     645     243
 
PasI CC'CwG_GG

Cuts at:      0    1416    2577    3126 
   Size:       1416    1161     549

  Fragments arranged by size: 

                1416    1161     549
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     230     578    1074    1416    3126 
   Size:        230     348     496     342    1710

  Fragments arranged by size: 

                1710     496     348     342     230
 
PfoI T'CCnGG_A

Cuts at:      0     677    1073    1622    3126 
   Size:        677     396     549    1504

  Fragments arranged by size: 

                1504     677     549     396
 
PmlI CAC'GTG

Cuts at:      0    1821    3126 
   Size:       1821    1305
 
PpuMI rG'GwC_Cy

Cuts at:      0     681    1196    2256    3126 
   Size:        681     515    1060     870

  Fragments arranged by size: 

                1060     870     681     515
 
PspOMI G'GGCC_C

Cuts at:      0    2844    3126 
   Size:       2844     282
 
PstI C_TGCA'G

Cuts at:      0    1637    1842    2028    2148    2312    3126 
   Size:       1637     205     186     120     164     814

  Fragments arranged by size: 

                1637     814     205     186     164     120
 
PvuII CAG'CTG

Cuts at:      0     578    1306    1599    3126 
   Size:        578     728     293    1527

  Fragments arranged by size: 

                1527     728     578     293
 
SacI G_AGCT'C

Cuts at:      0    2075    2506    2999    3126 
   Size:       2075     431     493     127

  Fragments arranged by size: 

                2075     493     431     127
 
SacII CC_GC'GG

Cuts at:      0     180    1000    1377    2676    3126 
   Size:        180     820     377    1299     450

  Fragments arranged by size: 

                1299     820     450     377     180
 
SanDI GG'GwC_CC

Cuts at:      0    1196    3126 
   Size:       1196    1930
 
SapI GCTCTTCn'nnn_

Cuts at:      0     124    3126 
   Size:        124    3002
 
ScaI AGT'ACT

Cuts at:      0     364    3126 
   Size:        364    2762
 
SexAI A'CCwGG_T

Cuts at:      0      91     161    2900    3126 
   Size:         91      70    2739     226

  Fragments arranged by size: 

                2739     226      91      70
 
SfcI C'TryA_G

Cuts at:      0    1633    1769    1838    2024    2144    2308    3126 
   Size:       1633     136      69     186     120     164     818

  Fragments arranged by size: 

                1633     818     186     164     136     120      69
 
SfoI GGC'GCC

Cuts at:      0    1066    2701    3126 
   Size:       1066    1635     425

  Fragments arranged by size: 

                1635    1066     425
 
SmaI CCC'GGG

Cuts at:      0       6    3126 
   Size:          6    3120
 
SmlI C'TyrA_G

Cuts at:      0     838    1474    3126 
   Size:        838     636    1652

  Fragments arranged by size: 

                1652     838     636
 
SphI G_CATG'C

Cuts at:      0     243    3126 
   Size:        243    2883
 
StuI AGG'CCT

Cuts at:      0     148    1354    1906    3126 
   Size:        148    1206     552    1220

  Fragments arranged by size: 

                1220    1206     552     148
 
StyI C'CwwG_G

Cuts at:      0    1208    1535    2030    2336    3126 
   Size:       1208     327     495     306     790

  Fragments arranged by size: 

                1208     790     495     327     306
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     953    3126 
   Size:        953    2173
 
TatI w'GTAC_w

Cuts at:      0     362    1310    2366    3126 
   Size:        362     948    1056     760

  Fragments arranged by size: 

                1056     948     760     362
 
Tth111I GACn'n_nGTC

Cuts at:      0      52    1102    1200    1573    3126 
   Size:         52    1050      98     373    1553

  Fragments arranged by size: 

                1553    1050     373      98      52
 
XmaI C'CCGG_G

Cuts at:      0       4    3126 
   Size:          4    3122
 

 Enzymes that do cut:

     AarI     AccI     AhdI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BglII    BmgBI     BmtI    BsiWI    BsmBI    BspHI   BssHII
    BstBI  BstZ17I    BtgZI     ClaI     DraI     DrdI    EcoRV     FalI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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