Restriction map for pF1KSDA0655
 (Linear) (Six-base) MAPSORT of: KIAA0655.seq  Check: 6838  from: 1  to: 3204



KIAA0655.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1482    1949    2179    2940    3204 
   Size:       1482     467     230     761     264

  Fragments arranged by size: 

                1482     761     467     264     230
 
AccI GT'mk_AC

Cuts at:      0     395    2348    3204 
   Size:        395    1953     856

  Fragments arranged by size: 

                1953     856     395
 
Acc65I G'GTAC_C

Cuts at:      0     320    3204 
   Size:        320    2884
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1171    1369    2266    2947    3204 
   Size:        580     591     198     897     681     257

  Fragments arranged by size: 

                 897     681     591     580     257     198
 
AgeI A'CCGG_T

Cuts at:      0     752    3204 
   Size:        752    2452
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1843    1875    3204 
   Size:       1843      32    1329

  Fragments arranged by size: 

                1843    1329      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1293    1725    1833    1845    2034    2232    2298    3204 
   Size:       1293     432     108      12     189     198      66     906

  Fragments arranged by size: 

                1293     906     432     198     189     108      66      12
            
 
ApaI G_GGCC'C

Cuts at:      0     974    1983    2190    3204 
   Size:        974    1009     207    1014

  Fragments arranged by size: 

                1014    1009     974     207
 
ApaLI G'TGCA_C

Cuts at:      0    1378    3204 
   Size:       1378    1826
 
ApoI r'AATT_y

Cuts at:      0    2578    3204 
   Size:       2578     626
 
AvaI C'yCGr_G

Cuts at:      0     847     916    2189    2436    3204 
   Size:        847      69    1273     247     768

  Fragments arranged by size: 

                1273     847     768     247      69
 
BamHI G'GATC_C

Cuts at:      0    2465    3204 
   Size:       2465     739
 
BanI G'GyrC_C

Cuts at:      0     290     320    2119    3204 
   Size:        290      30    1799    1085

  Fragments arranged by size: 

                1799    1085     290      30
 
BanII G_rGCy'C

Cuts at:      0     974    1370    1639    1983    2190    2196    2204    2708 
   Size:        974     396     269     344     207       6       8     504

Cuts at:   2708    3204 
   Size:        496

  Fragments arranged by size: 

                 974     504     496     396     344     269     207       8
                   6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2980    3204 
   Size:       2980     224
 
BbvCI CC'TCA_GC

Cuts at:      0     876    1255    3204 
   Size:        876     379    1949

  Fragments arranged by size: 

                1949     876     379
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     618    2094    3204 
   Size:        618    1476    1110

  Fragments arranged by size: 

                1476    1110     618
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1330    1987    2065    2629    2740    3204 
   Size:       1330     657      78     564     111     464

  Fragments arranged by size: 

                1330     657     564     464     111      78
 
BglII A'GATC_T

Cuts at:      0     419    3204 
   Size:        419    2785
 
BlpI GC'TnA_GC

Cuts at:      0      34     887    1633    1750    3204 
   Size:         34     853     746     117    1454

  Fragments arranged by size: 

                1454     853     746     117      34
 
Bme1580I G_kGCm'C

Cuts at:      0     172     293     974    1382    1983    2190    3204 
   Size:        172     121     681     408     601     207    1014

  Fragments arranged by size: 

                1014     681     601     408     207     172     121
 
BmgBI CAC'GTC

Cuts at:      0     264    3204 
   Size:        264    2940
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     438    2022    3204 
   Size:        438    1584    1182

  Fragments arranged by size: 

                1584    1182     438
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1653    1835    3204 
   Size:       1653     182    1369

  Fragments arranged by size: 

                1653    1369     182
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     481     502    1045    1129    1144    1192    1204 
   Size:         79     402      21     543      84      15      48      12

Cuts at:   1204    1291    1561    1582    2032    2218    2470    2983    2989 
   Size:         87     270      21     450     186     252     513       6

Cuts at:   2989    3001    3074    3204 
   Size:         12      73     130

  Fragments arranged by size: 

                 543     513     450     402     270     252     186     130
                  87      84      79      73      48      21      21      15
                  12      12       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     876    1255    1603    2794    3204 
   Size:        876     379     348    1191     410

  Fragments arranged by size: 

                1191     876     410     379     348
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1534    3204 
   Size:        418    1116    1670

  Fragments arranged by size: 

                1670    1116     418
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     341    3204 
   Size:        341    2863
 
BsaAI yAC'GTr

Cuts at:      0    3027    3204 
   Size:       3027     177
 
BsaHI Gr'CG_yC

Cuts at:      0    2051    3204 
   Size:       2051    1153
 
BsaWI w'CCGG_w

Cuts at:      0     752    3204 
   Size:        752    2452
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1495    1525    2023    2053    3047    3077    3204 
   Size:       1495      30     498      30     994      30     127

  Fragments arranged by size: 

                1495     994     498     127      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     934     952    1201    1315    1462    1537    2029    2344 
   Size:        934      18     249     114     147      75     492     315

Cuts at:   2344    2716    3073    3204 
   Size:        372     357     131

  Fragments arranged by size: 

                 934     492     372     357     315     249     147     131
                 114      75      18
 
BseYI C'CCAG_C

Cuts at:      0     229     493    1130    1250    1265    2286    2334    2639 
   Size:        229     264     637     120      15    1021      48     305

Cuts at:   2639    2744    3000    3034    3076    3130    3183    3204 
   Size:        105     256      34      42      54      53      21

  Fragments arranged by size: 

                1021     637     305     264     256     229     120     105
                  54      53      48      42      34      21      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1921    2463    3204 
   Size:       1921     542     741

  Fragments arranged by size: 

                1921     741     542
 
BsiEI CG_ry'CG

Cuts at:      0    2157    3204 
   Size:       2157    1047
 
BsiHKAI G_wGCw'C

Cuts at:      0     893    1370    1382    1639    2008    2204    2708    3204 
   Size:        893     477      12     257     369     196     504     496

  Fragments arranged by size: 

                 893     504     496     477     369     257     196      12
            
 
BsmI GAATG_Cn'

Cuts at:      0    1170    3204 
   Size:       1170    2034
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     278    2938    3204 
   Size:        278    2660     266

  Fragments arranged by size: 

                2660     278     266
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     293     893     974    1370    1382    1639    1983 
   Size:        172     121     600      81     396      12     257     344

Cuts at:   1983    2008    2190    2196    2204    2708    3204 
   Size:         25     182       6       8     504     496

  Fragments arranged by size: 

                 600     504     496     396     344     257     182     172
                 121      81      25      12       8       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1482    1715    1949    2179    2940    3204 
   Size:       1482     233     234     230     761     264

  Fragments arranged by size: 

                1482     761     264     234     233     230
 
BsrBI CCG'CTC

Cuts at:      0    1100    1281    2552    3204 
   Size:       1100     181    1271     652

  Fragments arranged by size: 

                1271    1100     652     181
 
BsrFI r'CCGG_y

Cuts at:      0      21     752     900    1799    3204 
   Size:         21     731     148     899    1405

  Fragments arranged by size: 

                1405     899     731     148      21
 
BssHII G'CGCG_C

Cuts at:      0    1333    1589    3204 
   Size:       1333     256    1615

  Fragments arranged by size: 

                1615    1333     256
 
BssSI C'ACGA_G

Cuts at:      0     178     760    1240    2722    3193    3204 
   Size:        178     582     480    1482     471      11

  Fragments arranged by size: 

                1482     582     480     471     178      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1833    1845    2180    2232    3204 
   Size:       1833      12     335      52     972

  Fragments arranged by size: 

                1833     972     335      52      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2634    2655    2847    3204 
   Size:       2634      21     192     357

  Fragments arranged by size: 

                2634     357     192      21
 
BstYI r'GATC_y

Cuts at:      0     419     833    2465    3204 
   Size:        419     414    1632     739

  Fragments arranged by size: 

                1632     739     419     414
 
Bsu36I CC'TnA_GG

Cuts at:      0     621     778    3204 
   Size:        621     157    2426

  Fragments arranged by size: 

                2426     621     157
 
BtgI C'CryG_G

Cuts at:      0     452     977    1325    1892    2342    2894    3204 
   Size:        452     525     348     567     450     552     310

  Fragments arranged by size: 

                 567     552     525     452     450     348     310
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     585    1125    3204 
   Size:        585     540    2079

  Fragments arranged by size: 

                2079     585     540
 
BtsI GCAGTG_nn'

Cuts at:      0    1871    3080    3204 
   Size:       1871    1209     124

  Fragments arranged by size: 

                1871    1209     124
 
DraIII CAC_nnn'GTG

Cuts at:      0    3027    3204 
   Size:       3027     177
 
EaeI y'GGCC_r

Cuts at:      0     596    2363    3065    3204 
   Size:        596    1767     702     139

  Fragments arranged by size: 

                1767     702     596     139
 
EarI CTCTTCn'nnn_

Cuts at:      0    1012    1072    1147    1522    3204 
   Size:       1012      60      75     375    1682

  Fragments arranged by size: 

                1682    1012     375      75      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1225    2046    3204 
   Size:       1225     821    1158

  Fragments arranged by size: 

                1225    1158     821
 
EcoICRI GAG'CTC

Cuts at:      0    1368    1637    2202    2706    3204 
   Size:       1368     269     565     504     498

  Fragments arranged by size: 

                1368     565     504     498     269
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     481     502     580    1045    1129    1144    1171 
   Size:         79     402      21      78     465      84      15      27

Cuts at:   1171    1192    1204    1291    1369    1561    1582    2032    2218 
   Size:         21      12      87      78     192      21     450     186

Cuts at:   2218    2266    2470    2947    2983    2989    3001    3074    3204 
   Size:         48     204     477      36       6      12      73     130

  Fragments arranged by size: 

                 477     465     450     402     204     192     186     130
                  87      84      79      78      78      73      48      36
                  27      21      21      21      15      12      12       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271     649    1297    2014    2491    2533    3204 
   Size:        271     378     648     717     477      42     671

  Fragments arranged by size: 

                 717     671     648     477     378     271      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     276     853     924     970     971    1056    1212 
   Size:        129     147     577      71      46       1      85     156

Cuts at:   1212    1290    1599    1979    2186    3204 
   Size:         78     309     380     207    1018

  Fragments arranged by size: 

                1018     577     380     309     207     156     147     129
                  85      78      71      46       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     441    3204 
   Size:        441    2763
 
HaeII r_GCGC'y

Cuts at:      0    1750    1880    3204 
   Size:       1750     130    1324

  Fragments arranged by size: 

                1750    1324     130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1801    1833    3204 
   Size:       1801      32    1371

  Fragments arranged by size: 

                1801    1371      32
 
HincII GTy'rAC

Cuts at:      0     507    2349    3204 
   Size:        507    1842     855

  Fragments arranged by size: 

                1842     855     507
 
HindIII A'AGCT_T

Cuts at:      0     562    3204 
   Size:        562    2642
 
KpnI G_GTAC'C

Cuts at:      0     324    3204 
   Size:        324    2880
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     606     827    1487    1607    3204 
   Size:        606     221     660     120    1597

  Fragments arranged by size: 

                1597     660     606     221     120
 
MscI TGG'CCA

Cuts at:      0    3067    3204 
   Size:       3067     137
 
MslI CAynn'nnrTG

Cuts at:      0     296     365    3204 
   Size:        296      69    2839

  Fragments arranged by size: 

                2839     296      69
 
MspA1I CmG'CkG

Cuts at:      0     225     248     318     384     454     493     979    1134 
   Size:        225      23      70      66      70      39     486     155

Cuts at:   1134    1149    1194    1254    1269    1422    1473    1539    1695 
   Size:         15      45      60      15     153      51      66     156

Cuts at:   1695    1894    2214    2286    2655    2668    2748    3204 
   Size:        199     320      72     369      13      80     456

  Fragments arranged by size: 

                 486     456     369     320     225     199     156     155
                 153      80      72      70      70      66      66      60
                  51      45      39      23      15      15      13
 
NaeI GCC'GGC

Cuts at:      0      23    1801    3204 
   Size:         23    1778    1403

  Fragments arranged by size: 

                1778    1403      23
 
NcoI C'CATG_G

Cuts at:      0    2894    3204 
   Size:       2894     310
 
NgoMIV G'CCGG_C

Cuts at:      0      21    1799    3204 
   Size:         21    1778    1405

  Fragments arranged by size: 

                1778    1405      21
 
NspI r_CATG'y

Cuts at:      0     810    2244    2997    3204 
   Size:        810    1434     753     207

  Fragments arranged by size: 

                1434     810     753     207
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57    1245    2751    3204 
   Size:         57    1188    1506     453

  Fragments arranged by size: 

                1506    1188     453      57
 
PfoI T'CCnGG_A

Cuts at:      0     335     659    2960    3204 
   Size:        335     324    2301     244

  Fragments arranged by size: 

                2301     335     324     244
 
PpuMI rG'GwC_Cy

Cuts at:      0     276     853    1056    3204 
   Size:        276     577     203    2148

  Fragments arranged by size: 

                2148     577     276     203
 
PshAI GACnn'nnGTC

Cuts at:      0     502    3204 
   Size:        502    2702
 
PspOMI G'GGCC_C

Cuts at:      0     970    1979    2186    3204 
   Size:        970    1009     207    1018

  Fragments arranged by size: 

                1018    1009     970     207
 
PstI C_TGCA'G

Cuts at:      0     670    1907    2176    2219    2273    2534    2807    3204 
   Size:        670    1237     269      43      54     261     273     397

  Fragments arranged by size: 

                1237     670     397     273     269     261      54      43
            
 
PvuII CAG'CTG

Cuts at:      0     248     384     493    1149    1254    1269    1422    1473 
   Size:        248     136     109     656     105      15     153      51

Cuts at:   1473    1539    2214    2286    2655    2668    2748    3204 
   Size:         66     675      72     369      13      80     456

  Fragments arranged by size: 

                 675     656     456     369     248     153     136     109
                 105      80      72      66      51      15      13
 
SacI G_AGCT'C

Cuts at:      0    1370    1639    2204    2708    3204 
   Size:       1370     269     565     504     496

  Fragments arranged by size: 

                1370     565     504     496     269
 
SacII CC_GC'GG

Cuts at:      0     455     980    1895    3204 
   Size:        455     525     915    1309

  Fragments arranged by size: 

                1309     915     525     455
 
SalI G'TCGA_C

Cuts at:      0    2347    3204 
   Size:       2347     857
 
SanDI GG'GwC_CC

Cuts at:      0     853    3204 
   Size:        853    2351
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1147    1522    3204 
   Size:       1147     375    1682

  Fragments arranged by size: 

                1682    1147     375
 
SbfI CC_TGCA'GG

Cuts at:      0    1907    2176    2273    2807    3204 
   Size:       1907     269      97     534     397

  Fragments arranged by size: 

                1907     534     397     269      97
 
SexAI A'CCwGG_T

Cuts at:      0    1967    3204 
   Size:       1967    1237
 
SfcI C'TryA_G

Cuts at:      0     666    1903    2172    2215    2269    2530    2803    3204 
   Size:        666    1237     269      43      54     261     273     401

  Fragments arranged by size: 

                1237     666     401     273     269     261      54      43
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1987    3204 
   Size:       1987    1217
 
SmaI CCC'GGG

Cuts at:      0    2191    3204 
   Size:       2191    1013
 
SmlI C'TyrA_G

Cuts at:      0     433    1549    3204 
   Size:        433    1116    1655

  Fragments arranged by size: 

                1655    1116     433
 
SphI G_CATG'C

Cuts at:      0    2997    3204 
   Size:       2997     207
 
SrfI GCCC'GGGC

Cuts at:      0    2191    3204 
   Size:       2191    1013
 
StuI AGG'CCT

Cuts at:      0    1990    2612    3204 
   Size:       1990     622     592

  Fragments arranged by size: 

                1990     622     592
 
StyI C'CwwG_G

Cuts at:      0    1568    1991    2042    2627    2762    2894    3204 
   Size:       1568     423      51     585     135     132     310

  Fragments arranged by size: 

                1568     585     423     310     135     132      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     136     514    2620    3204 
   Size:        136     378    2106     584

  Fragments arranged by size: 

                2106     584     378     136
 
TatI w'GTAC_w

Cuts at:      0     812    3204 
   Size:        812    2392
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     371     403    1305    1337    3047    3079    3204 
   Size:        371      32     902      32    1710      32     125

  Fragments arranged by size: 

                1710     902     371     125      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2130    2655    3204 
   Size:       2130     525     549

  Fragments arranged by size: 

                2130     549     525
 
XmaI C'CCGG_G

Cuts at:      0    2189    3204 
   Size:       2189    1015
 
XmnI GAAnn'nnTTC

Cuts at:      0     787    3204 
   Size:        787    2417
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AgeI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AhdI     AleI     AlfI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI     BclI
    BfrBI     BmtI    BsaBI    BsiWI    BspEI    BspHI    BsrDI    BsrGI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI
    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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