Restriction map for pF1KSDA0615
 (Linear) (Six-base) MAPSORT of: KIAA0615.seq  Check: 5763  from: 1  to: 2688



KIAA0615.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AclI AA'CG_TT

Cuts at:      0    1632    2688 
   Size:       1632    1056
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     890    2296    2373    2605    2688 
   Size:        890    1406      77     232      83

  Fragments arranged by size: 

                1406     890     232      83      77
 
AflIII A'CryG_T

Cuts at:      0    2237    2688 
   Size:       2237     451
 
AleI CACnn'nnGTG

Cuts at:      0    2242    2688 
   Size:       2242     446
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     172    2688 
   Size:        172    2516
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1459    1771    2526    2688 
   Size:       1459     312     755     162

  Fragments arranged by size: 

                1459     755     312     162
 
ApoI r'AATT_y

Cuts at:      0     475     839     996    1186    1321    2179    2688 
   Size:        475     364     157     190     135     858     509

  Fragments arranged by size: 

                 858     509     475     364     190     157     135
 
AseI AT'TA_AT

Cuts at:      0    1379    2688 
   Size:       1379    1309
 
AvaI C'yCGr_G

Cuts at:      0       8    2688 
   Size:          8    2680
 
BanI G'GyrC_C

Cuts at:      0    2401    2688 
   Size:       2401     287
 
BanII G_rGCy'C

Cuts at:      0    2051    2454    2688 
   Size:       2051     403     234

  Fragments arranged by size: 

                2051     403     234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1184    2688 
   Size:       1184    1504
 
BbvCI CC'TCA_GC

Cuts at:      0     128     371    2474    2569    2688 
   Size:        128     243    2103      95     119

  Fragments arranged by size: 

                2103     243     128     119      95
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      13      47    2688 
   Size:         13      34    2641

  Fragments arranged by size: 

                2641      34      13
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     306    1217    2688 
   Size:        306     911    1471

  Fragments arranged by size: 

                1471     911     306
 
BclI T'GATC_A

Cuts at:      0    1749    2688 
   Size:       1749     939
 
BglI GCCn_nnn'nGGC

Cuts at:      0      92     353    2688 
   Size:         92     261    2335

  Fragments arranged by size: 

                2335     261      92
 
BglII A'GATC_T

Cuts at:      0    2545    2588    2627    2655    2688 
   Size:       2545      43      39      28      33

  Fragments arranged by size: 

                2545      43      39      33      28
 
Bme1580I G_kGCm'C

Cuts at:      0    2358    2688 
   Size:       2358     330
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     437    1781    2504    2688 
   Size:        437    1344     723     184

  Fragments arranged by size: 

                1344     723     437     184
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     644    2214    2688 
   Size:        644    1570     474

  Fragments arranged by size: 

                1570     644     474
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    2026    2500    2688 
   Size:         79    1947     474     188

  Fragments arranged by size: 

                1947     474     188      79
 
Bpu10I CC'TnA_GC

Cuts at:      0     128     371    2474    2569    2688 
   Size:        128     243    2103      95     119

  Fragments arranged by size: 

                2103     243     128     119      95
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1207    2688 
   Size:       1207    1481
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1668    1698    2688 
   Size:       1668      30     990

  Fragments arranged by size: 

                1668     990      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     592    1643    2317    2688 
   Size:        145     447    1051     674     371

  Fragments arranged by size: 

                1051     674     447     371     145
 
BseYI C'CCAG_C

Cuts at:      0     718    1703    2036    2383    2688 
   Size:        718     985     333     347     305

  Fragments arranged by size: 

                 985     718     347     333     305
 
BsiEI CG_ry'CG

Cuts at:      0      75    2688 
   Size:         75    2613
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     188    2051    2688 
   Size:        113      75    1863     637

  Fragments arranged by size: 

                1863     637     113      75
 
BsmI GAATG_Cn'

Cuts at:      0     332     657    2688 
   Size:        332     325    2031

  Fragments arranged by size: 

                2031     332     325
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     188    2051    2358    2454    2688 
   Size:        113      75    1863     307      96     234

  Fragments arranged by size: 

                1863     307     234     113      96      75
 
BspHI T'CATG_A

Cuts at:      0    2106    2688 
   Size:       2106     582
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2534    2688 
   Size:       2534     154
 
BsrDI GCAATG_nn'

Cuts at:      0    1881    2688 
   Size:       1881     807
 
BssHII G'CGCG_C

Cuts at:      0     116     145    2688 
   Size:        116      29    2543

  Fragments arranged by size: 

                2543     116      29
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2526    2688 
   Size:       2526     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2682    2688 
   Size:       2682       6
 
BstYI r'GATC_y

Cuts at:      0    2545    2588    2627    2655    2688 
   Size:       2545      43      39      28      33

  Fragments arranged by size: 

                2545      43      39      33      28
 
BtgI C'CryG_G

Cuts at:      0      69    1067    1890    2675    2688 
   Size:         69     998     823     785      13

  Fragments arranged by size: 

                 998     823     785      69      13
 
BtsI GCAGTG_nn'

Cuts at:      0      29     261    1696    2491    2688 
   Size:         29     232    1435     795     197

  Fragments arranged by size: 

                1435     795     232     197      29
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2127    2162    2688 
   Size:       2127      35     526

  Fragments arranged by size: 

                2127     526      35
 
DraI TTT'AAA

Cuts at:      0    1062    2688 
   Size:       1062    1626
 
DraIII CAC_nnn'GTG

Cuts at:      0     574    2688 
   Size:        574    2114
 
EaeI y'GGCC_r

Cuts at:      0      72    2688 
   Size:         72    2616
 
EagI C'GGCC_G

Cuts at:      0      72    2688 
   Size:         72    2616
 
EarI CTCTTCn'nnn_

Cuts at:      0     695     812    2688 
   Size:        695     117    1876

  Fragments arranged by size: 

                1876     695     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     196    2688 
   Size:         64     132    2492

  Fragments arranged by size: 

                2492     132      64
 
EcoICRI GAG'CTC

Cuts at:      0    2049    2688 
   Size:       2049     639
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     890    2026    2296    2373    2500    2605    2688 
   Size:         79     811    1136     270      77     127     105      83

  Fragments arranged by size: 

                1136     811     270     127     105      83      79      77
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84    1741    2688 
   Size:         84    1657     947

  Fragments arranged by size: 

                1657     947      84
 
EcoRI G'AATT_C

Cuts at:      0     475    2688 
   Size:        475    2213
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     841    1547    2117    2344    2688 
   Size:        841     706     570     227     344

  Fragments arranged by size: 

                 841     706     570     344     227
 
HaeII r_GCGC'y

Cuts at:      0     194    2688 
   Size:        194    2494
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     828     860    1717    1749    2688 
   Size:        828      32     857      32     939

  Fragments arranged by size: 

                 939     857     828      32      32
 
HindIII A'AGCT_T

Cuts at:      0     300    1951    2688 
   Size:        300    1651     737

  Fragments arranged by size: 

                1651     737     300
 
MfeI C'AATT_G

Cuts at:      0    1928    2688 
   Size:       1928     760
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2458    2663    2688 
   Size:       2458     205      25

  Fragments arranged by size: 

                2458     205      25
 
MslI CAynn'nnrTG

Cuts at:      0    1105    2155    2242    2257    2688 
   Size:       1105    1050      87      15     431

  Fragments arranged by size: 

                1105    1050     431      87      15
 
MspA1I CmG'CkG

Cuts at:      0      40      71     138    1195    1707    2688 
   Size:         40      31      67    1057     512     981

  Fragments arranged by size: 

                1057     981     512      67      40      31
 
NcoI C'CATG_G

Cuts at:      0    1067    1890    2675    2688 
   Size:       1067     823     785      13

  Fragments arranged by size: 

                1067     823     785      13
 
NspI r_CATG'y

Cuts at:      0     261    2376    2688 
   Size:        261    2115     312

  Fragments arranged by size: 

                2115     312     261
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     799    2631    2688 
   Size:        799    1832      57

  Fragments arranged by size: 

                1832     799      57
 
PfoI T'CCnGG_A

Cuts at:      0    2042    2688 
   Size:       2042     646
 
PpuMI rG'GwC_Cy

Cuts at:      0    1741    2688 
   Size:       1741     947
 
PstI C_TGCA'G

Cuts at:      0     161    2234    2556    2688 
   Size:        161    2073     322     132

  Fragments arranged by size: 

                2073     322     161     132
 
PvuII CAG'CTG

Cuts at:      0      40    1195    1707    2688 
   Size:         40    1155     512     981

  Fragments arranged by size: 

                1155     981     512      40
 
SacI G_AGCT'C

Cuts at:      0    2051    2688 
   Size:       2051     637
 
SacII CC_GC'GG

Cuts at:      0      72    2688 
   Size:         72    2616
 
SfcI C'TryA_G

Cuts at:      0     157    2230    2552    2688 
   Size:        157    2073     322     136

  Fragments arranged by size: 

                2073     322     157     136
 
SmaI CCC'GGG

Cuts at:      0      10    2688 
   Size:         10    2678
 
SrfI GCCC'GGGC

Cuts at:      0      10    2688 
   Size:         10    2678
 
SspI AAT'ATT

Cuts at:      0     672    1942    2057    2688 
   Size:        672    1270     115     631

  Fragments arranged by size: 

                1270     672     631     115
 
StyI C'CwwG_G

Cuts at:      0     194     251    1067    1890    2675    2688 
   Size:        194      57     816     823     785      13

  Fragments arranged by size: 

                 823     816     785     194      57      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2419    2657    2688 
   Size:       2419     238      31

  Fragments arranged by size: 

                2419     238      31
 
TatI w'GTAC_w

Cuts at:      0    2234    2688 
   Size:       2234     454
 
Tth111I GACn'n_nGTC

Cuts at:      0     757    2688 
   Size:        757    1931
 
XbaI T'CTAG_A

Cuts at:      0     676    2688 
   Size:        676    2012
 
XmaI C'CCGG_G

Cuts at:      0       8    2688 
   Size:          8    2680
 
XmnI GAAnn'nnTTC

Cuts at:      0     908    1903    2100    2579    2590    2688 
   Size:        908     995     197     479      11      98

  Fragments arranged by size: 

                 995     908     479     197      98      11
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI   BssHII   BstAPI    BstXI    BstYI     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I  HindIII     MfeI
     MmeI     MslI   MspA1I     NcoI     NspI    PflMI     PfoI    PpuMI
     PstI    PvuII     SacI    SacII     SfcI     SmaI     SrfI     SspI
     StyI    TaqII     TatI  Tth111I     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BfrBI     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI    EcoNI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     StuI
     SwaI     TstI     XcmI     XhoI     ZraI
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