Restriction map for pF1KSDA0552
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AlwI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BlnI        BpuAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I        166  GGTACC               G^GTACC
Alw44I        953  GTGCAC               G^TGCAC
AocI          559  CCTGAGG              CC^T[ACGT]AGG
ApaLI         953  GTGCAC               G^TGCAC
Asp718I       166  GGTACC               G^GTACC
BcgI         1530  CGAGCTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1343  AGATCT               A^GATCT
BsaAI        1867  TACGTG               [CT]AC^GT[GA]
BsaI          274  GGTCTC               GGTCTC(1/5)
BsmBI          13  GAGACG               CGTCTC(1/5)
BsrFI        1554  GCCGGC               [GA]^CCGG[CT]
BstYI        1343  AGATCT               [GA]^GATC[CT]
Bsu36I        559  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1554  GCCGGC               [GA]^CCGG[CT]
Eco81I        559  CCTGAGG              CC^T[ACGT]AGG
KpnI          166  GGTACC               GGTAC^C
MflI         1343  AGATCT               [GA]^GATC[CT]
MseI          743  TTAA                 T^TAA
MslI          322  CACACCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1554  GCCGGC               GCC^GGC
NcoI          107  CCATGG               C^CATGG
NgoMIV       1554  GCCGGC               G^CCGGC
PaeR7I       1993  CTCGAG               C^TCGAG
PshAI         268  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI         1769  CCCGGG               CCC^GGG
SpeI          471  ACTAGT               A^CTAGT
Tru9I         743  TTAA                 T^TAA
XhoI         1993  CTCGAG               C^TCGAG
XhoII        1343  AGATCT               [GA]^GATC[CT]
XmaCI        1769  CCCGGG               C^CCGGG
XmaI         1769  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          510  GAATTT               [GA]^AATT[CT]
              528  GAATTT              
ApoI          510  GAATTT               [GA]^AATT[CT]
              528  GAATTT              
AspHI         459  GTGCTC               G[AT]GC[AT]^C
              953  GTGCAC              
BfaI          451  CTAG                 C^TAG
              472  CTAG                
BsiEI         478  CGGCCG               CG[GA][CT]^CG
             1577  CGGCCG              
BsiHKAI       459  GTGCTC               G[AT]GC[AT]^C
              953  GTGCAC              
BssHII         27  GCGCGC               G^CGCGC
             1149  GCGCGC              
BstAPI       1173  GCAGGTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1927  GCAGGGGGTGC         
BstXI         425  CCAAGCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1329  CCAGAAGGCTGG        
EagI          478  CGGCCG               C^GGCCG
             1577  CGGCCG              
EarI          156  GAAGAG               CTCTTC(1/4)
             1243  GAAGAG              
EclXI         478  CGGCCG               C^GGCCG
             1577  CGGCCG              
Eco52I        478  CGGCCG               C^GGCCG
             1577  CGGCCG              
EcoT14I       107  CCATGG               C^C[AT][AT]GG
             1661  CCTTGG              
HgaI           15  GACGC                GACGC(5/10)
             1707  GACGC               
Ksp632I       156  GAAGAG               CTCTTC(1/4)
             1243  GAAGAG              
MaeI          451  CTAG                 C^TAG
              472  CTAG                
MaeIII        317  GTGAC                ^GT[ACGT]AC
              383  GTGAC               
SapI          156  GAAGAGC              GCTCTTC(1/4)
             1243  GAAGAGC             
SfiI          574  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1638  GGCCGGGGTGGCC       
SmlI         1549  CTCAAG               C^T[CT][GA]AG
             1993  CTCGAG              
StuI          915  AGGCCT               AGG^CCT
             1973  AGGCCT              
StyI          107  CCATGG               C^C[AT][AT]GG
             1661  CCTTGG              
TaqI         1838  TCGA                 T^CGA
             1994  TCGA                
Tsp45I        317  GTGAC                ^GT[GC]AC
              383  GTGAC               
Tsp509I       511  AATT                 ^AATT
              529  AATT                
TthHB8I      1838  TCGA                 T^CGA
             1994  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
ApaI        AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MboI        MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        TspRI       Van91I      
XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com