Restriction map for pF1KSDA0552
 (Linear) (Six-base) MAPSORT of: KIAA0552.seq  Check: 4352  from: 1  to: 2019



KIAA0552.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1119    1164    1612    2019 
   Size:       1119      45     448     407

  Fragments arranged by size: 

                1119     448     407      45
 
Acc65I G'GTAC_C

Cuts at:      0     166    2019 
   Size:        166    1853
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1375    1387    1476    2019 
   Size:       1375      12      89     543

  Fragments arranged by size: 

                1375     543      89      12
 
AlwNI CAG_nnn'CTG

Cuts at:      0     711     816     969    1056    1365    1896    2019 
   Size:        711     105     153      87     309     531     123

  Fragments arranged by size: 

                 711     531     309     153     123     105      87
 
ApaI G_GGCC'C

Cuts at:      0     577    1716    1770    2019 
   Size:        577    1139      54     249

  Fragments arranged by size: 

                1139     577     249      54
 
ApaLI G'TGCA_C

Cuts at:      0     953    2019 
   Size:        953    1066
 
ApoI r'AATT_y

Cuts at:      0     510     528    2019 
   Size:        510      18    1491

  Fragments arranged by size: 

                1491     510      18
 
AvaI C'yCGr_G

Cuts at:      0     212    1769    1993    2019 
   Size:        212    1557     224      26

  Fragments arranged by size: 

                1557     224     212      26
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     385     418    2019 
   Size:        385      33    1601

  Fragments arranged by size: 

                1601     385      33
 
BanI G'GyrC_C

Cuts at:      0     166     714    1783    2019 
   Size:        166     548    1069     236

  Fragments arranged by size: 

                1069     548     236     166
 
BanII G_rGCy'C

Cuts at:      0     413     577     895    1716    1770    2019 
   Size:        413     164     318     821      54     249

  Fragments arranged by size: 

                 821     413     318     249     164      54
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1519    1553    2019 
   Size:       1519      34     466

  Fragments arranged by size: 

                1519     466      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     215     581     590     890    1645    1750    2019 
   Size:        215     366       9     300     755     105     269

  Fragments arranged by size: 

                 755     366     300     269     215     105       9
 
BglII A'GATC_T

Cuts at:      0    1343    2019 
   Size:       1343     676
 
BlpI GC'TnA_GC

Cuts at:      0    1474    1757    1861    2019 
   Size:       1474     283     104     158

  Fragments arranged by size: 

                1474     283     158     104
 
Bme1580I G_kGCm'C

Cuts at:      0     538     577     957    1146    1716    1770    2019 
   Size:        538      39     380     189     570      54     249

  Fragments arranged by size: 

                 570     538     380     249     189      54      39
 
BmgBI CAC'GTC

Cuts at:      0    1141    2019 
   Size:       1141     878
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     944    2019 
   Size:        944    1075
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     605     678     828     991    1045    1078    1270 
   Size:         31     574      73     150     163      54      33     192

Cuts at:   1270    1534    1837    1918    2019 
   Size:        264     303      81     101

  Fragments arranged by size: 

                 574     303     264     192     163     150     101      81
                  73      54      33      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1534    2019 
   Size:       1534     485
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     280    2019 
   Size:        280    1739
 
BsaAI yAC'GTr

Cuts at:      0    1869    2019 
   Size:       1869     150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     413     443     603     624     633     654     967     997 
   Size:        413      30     160      21       9      21     313      30

Cuts at:    997    1932    1962    2019 
   Size:        935      30      57

  Fragments arranged by size: 

                 935     413     313     160      57      30      30      30
                  21      21       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     988    1075    1228    1477    1813    1834    1978    2019 
   Size:        988      87     153     249     336      21     144      41

  Fragments arranged by size: 

                 988     336     249     153     144      87      41      21
            
 
BseYI C'CCAG_C

Cuts at:      0     412    1145    1154    1518    1952    2019 
   Size:        412     733       9     364     434      67

  Fragments arranged by size: 

                 733     434     412     364      67       9
 
BsiEI CG_ry'CG

Cuts at:      0     481    1580    2019 
   Size:        481    1099     439

  Fragments arranged by size: 

                1099     481     439
 
BsiHKAI G_wGCw'C

Cuts at:      0     463     957    2019 
   Size:        463     494    1062

  Fragments arranged by size: 

                1062     494     463
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       7    2019 
   Size:          7    2012
 
Bsp1286I G_dGCh'C

Cuts at:      0     413     463     538     577     895     957    1146    1716 
   Size:        413      50      75      39     318      62     189     570

Cuts at:   1716    1770    2019 
   Size:         54     249

  Fragments arranged by size: 

                 570     413     318     249     189      75      62      54
                  50      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1119    1164    1612    2019 
   Size:       1119      45     448     407

  Fragments arranged by size: 

                1119     448     407      45
 
BsrBI CCG'CTC

Cuts at:      0     978    1065    1083    1703    1761    2019 
   Size:        978      87      18     620      58     258

  Fragments arranged by size: 

                 978     620     258      87      58      18
 
BsrFI r'CCGG_y

Cuts at:      0    1554    2019 
   Size:       1554     465
 
BssHII G'CGCG_C

Cuts at:      0      27    1149    2019 
   Size:         27    1122     870

  Fragments arranged by size: 

                1122     870      27
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1179    1933    2019 
   Size:       1179     754      86

  Fragments arranged by size: 

                1179     754      86
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     432    1336    2019 
   Size:        432     904     683

  Fragments arranged by size: 

                 904     683     432
 
BstYI r'GATC_y

Cuts at:      0    1343    2019 
   Size:       1343     676
 
Bsu36I CC'TnA_GG

Cuts at:      0     560    2019 
   Size:        560    1459
 
BtgI C'CryG_G

Cuts at:      0      67     107    1568    1670    2019 
   Size:         67      40    1461     102     349

  Fragments arranged by size: 

                1461     349     102      67      40
 
BtsI GCAGTG_nn'

Cuts at:      0     129     225     231     321     378     387     755    2019 
   Size:        129      96       6      90      57       9     368    1264

  Fragments arranged by size: 

                1264     368     129      96      90      57       9       6
            
 
EaeI y'GGCC_r

Cuts at:      0     104     434     478     877    1091    1262    1577    1598 
   Size:        104     330      44     399     214     171     315      21

Cuts at:   1598    1646    2019 
   Size:         48     373

  Fragments arranged by size: 

                 399     373     330     315     214     171     104      48
                  44      21
 
EagI C'GGCC_G

Cuts at:      0     478    1577    2019 
   Size:        478    1099     442

  Fragments arranged by size: 

                1099     478     442
 
EarI CTCTTCn'nnn_

Cuts at:      0     151    1238    2019 
   Size:        151    1087     781

  Fragments arranged by size: 

                1087     781     151
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     608     920    1396    1699    2019 
   Size:        608     312     476     303     320

  Fragments arranged by size: 

                 608     476     320     312     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     605     678     828     991    1045    1078    1270 
   Size:         31     574      73     150     163      54      33     192

Cuts at:   1270    1375    1387    1476    1534    1837    1918    2019 
   Size:        105      12      89      58     303      81     101

  Fragments arranged by size: 

                 574     303     192     163     150     105     101      89
                  81      73      58      54      33      31      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     573     574     813     821    1161    1713    2019 
   Size:         45     528       1     239       8     340     552     306

  Fragments arranged by size: 

                 552     528     340     306     239      45       8       1
            
 
HaeII r_GCGC'y

Cuts at:      0    1561    1779    1806    2019 
   Size:       1561     218      27     213

  Fragments arranged by size: 

                1561     218     213      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     603     635    1617    1649    2019 
   Size:        445      32     126      32     982      32     370

  Fragments arranged by size: 

                 982     445     370     126      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     170    2019 
   Size:        170    1849
 
MscI TGG'CCA

Cuts at:      0     106     436    1600    2019 
   Size:        106     330    1164     419

  Fragments arranged by size: 

                1164     419     330     106
 
MslI CAynn'nnrTG

Cuts at:      0     326    2019 
   Size:        326    1693
 
MspA1I CmG'CkG

Cuts at:      0     356     625     791    1115    1170    1206    1239    1365 
   Size:        356     269     166     324      55      36      33     126

Cuts at:   1365    1428    1470    1651    1854    1860    1896    2019 
   Size:         63      42     181     203       6      36     123

  Fragments arranged by size: 

                 356     324     269     203     181     166     126     123
                  63      55      42      36      36      33       6
 
NaeI GCC'GGC

Cuts at:      0    1556    2019 
   Size:       1556     463
 
NcoI C'CATG_G

Cuts at:      0     107    2019 
   Size:        107    1912
 
NgoMIV G'CCGG_C

Cuts at:      0    1554    2019 
   Size:       1554     465
 
PasI CC'CwG_GG

Cuts at:      0      37    2019 
   Size:         37    1982
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     628     711     816     969    1056    1896    2019 
   Size:        628      83     105     153      87     840     123

  Fragments arranged by size: 

                 840     628     153     123     105      87      83
 
PfoI T'CCnGG_A

Cuts at:      0     962    2019 
   Size:        962    1057
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     813     821    2019 
   Size:         45     768       8    1198

  Fragments arranged by size: 

                1198     768      45       8
 
PshAI GACnn'nnGTC

Cuts at:      0     272    2019 
   Size:        272    1747
 
PspOMI G'GGCC_C

Cuts at:      0     573    1712    1766    2019 
   Size:        573    1139      54     253

  Fragments arranged by size: 

                1139     573     253      54
 
PspXI vC'TCGA_Gb

Cuts at:      0    1993    2019 
   Size:       1993      26
 
PstI C_TGCA'G

Cuts at:      0    1114    1175    1190    1211    1742    1859    2019 
   Size:       1114      61      15      21     531     117     160

  Fragments arranged by size: 

                1114     531     160     117      61      21      15
 
PvuII CAG'CTG

Cuts at:      0    1170    1206    1365    1428    1470    1854    1860    1896 
   Size:       1170      36     159      63      42     384       6      36

Cuts at:   1896    2019 
   Size:        123

  Fragments arranged by size: 

                1170     384     159     123      63      42      36      36
                   6
 
SanDI GG'GwC_CC

Cuts at:      0      45    2019 
   Size:         45    1974
 
SapI GCTCTTCn'nnn_

Cuts at:      0     151    1238    2019 
   Size:        151    1087     781

  Fragments arranged by size: 

                1087     781     151
 
SfcI C'TryA_G

Cuts at:      0     687     699    1110    1123    1165    1171    1186    1207 
   Size:        687      12     411      13      42       6      15      21

Cuts at:   1207    1738    1855    2019 
   Size:        531     117     164

  Fragments arranged by size: 

                 687     531     411     164     117      42      21      15
                  13      12       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     581    1645    2019 
   Size:        581    1064     374

  Fragments arranged by size: 

                1064     581     374
 
SmaI CCC'GGG

Cuts at:      0    1771    2019 
   Size:       1771     248
 
SmlI C'TyrA_G

Cuts at:      0    1549    1993    2019 
   Size:       1549     444      26

  Fragments arranged by size: 

                1549     444      26
 
SpeI A'CTAG_T

Cuts at:      0     471    2019 
   Size:        471    1548
 
StuI AGG'CCT

Cuts at:      0     917    1975    2019 
   Size:        917    1058      44

  Fragments arranged by size: 

                1058     917      44
 
StyI C'CwwG_G

Cuts at:      0     107    1661    2019 
   Size:        107    1554     358

  Fragments arranged by size: 

                1554     358     107
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     434     438    2019 
   Size:        434       4    1581

  Fragments arranged by size: 

                1581     434       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     413     445     624     656     967     999    1300    1332 
   Size:        413      32     179      32     311      32     301      32

Cuts at:   1332    1932    1964    2019 
   Size:        600      32      55

  Fragments arranged by size: 

                 600     413     311     301     179      55      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     114     159     892    1515    2019 
   Size:        114      45     733     623     504

  Fragments arranged by size: 

                 733     623     504     114      45
 
XhoI C'TCGA_G

Cuts at:      0    1993    2019 
   Size:       1993      26
 
XmaI C'CCGG_G

Cuts at:      0    1769    2019 
   Size:       1769     250
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI     BanI    BanII     BcgI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BssHII   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I     KpnI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI
     PfoI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII    SanDI
     SapI     SfcI     SfiI     SmaI     SmlI     SpeI     StuI     StyI
    TaqII     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BmtI     BplI
   Bpu10I    BsaBI    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XmnI     ZraI
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