(Circular) (Six-base) MAPSORT of: pF1KSDA0532.seq Check: 8853 from: 1 to: 7660 pF1KSDA0532 7660 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AccI GT'mk_AC Cuts at: 1878 3194 4696 1878 Size: 1316 1502 4842 Fragments arranged by size: 4842 1502 1316 Acc65I G'GTAC_C Cuts at: 3700 4674 3700 Size: 974 6686 AclI AA'CG_TT Cuts at: 7500 7500 Size: 7660 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 144 276 450 1277 2104 3588 3920 4144 5483 Size: 132 174 827 827 1484 332 224 1339 Cuts at: 5483 5915 6664 6932 144 Size: 432 749 268 872 Fragments arranged by size: 1484 1339 872 827 827 749 432 332 268 224 174 132 AflIII A'CryG_T Cuts at: 173 5010 6116 7093 173 Size: 4837 1106 977 740 Fragments arranged by size: 4837 1106 977 740 AgeI A'CCGG_T Cuts at: 6048 6048 Size: 7660 AhdI GACnn_n'nnGTC Cuts at: 4180 4472 4180 Size: 292 7368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 3880 3880 Size: 7660 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 183 205 215 237 183 Size: 22 10 22 7606 Fragments arranged by size: 7606 22 22 10 AlwNI CAG_nnn'CTG Cuts at: 2161 3843 4167 4963 6532 2161 Size: 1682 324 796 1569 3289 Fragments arranged by size: 3289 1682 1569 796 324 ApaI G_GGCC'C Cuts at: 1548 3510 3637 1548 Size: 1962 127 5571 Fragments arranged by size: 5571 1962 127 ApaLI G'TGCA_C Cuts at: 1053 1693 3475 4328 6430 1053 Size: 640 1782 853 2102 2283 Fragments arranged by size: 2283 2102 1782 853 640 ApoI r'AATT_y Cuts at: 1384 2241 2429 3360 4603 4662 7013 7269 1384 Size: 857 188 931 1243 59 2351 256 1775 Fragments arranged by size: 2351 1775 1243 931 857 256 188 59 AseI AT'TA_AT Cuts at: 20 3137 20 Size: 3117 4543 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7660 AvaI C'yCGr_G Cuts at: 2236 2339 2853 3629 3811 3966 4678 2236 Size: 103 514 776 182 155 712 5218 Fragments arranged by size: 5218 776 712 514 182 155 103 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 160 193 160 Size: 33 7627 BamHI G'GATC_C Cuts at: 4683 4683 Size: 7660 BanI G'GyrC_C Cuts at: 1017 3700 4674 5088 5336 5371 1017 Size: 2683 974 414 248 35 3306 Fragments arranged by size: 3306 2683 974 414 248 35 BanII G_rGCy'C Cuts at: 1548 3510 3637 4219 4672 5702 1548 Size: 1962 127 582 453 1030 3506 Fragments arranged by size: 3506 1962 1030 582 453 127 BbeI G_GCGC'C Cuts at: 5092 5340 5092 Size: 248 7412 BbsI GAAGACnn'nnnn_ Cuts at: 456 774 962 1904 2333 2652 3188 4584 456 Size: 318 188 942 429 319 536 1396 3532 Fragments arranged by size: 3532 1396 942 536 429 319 318 188 BciVI GTATCCnnnnn_n' Cuts at: 516 5556 6319 7660 516 Size: 5040 763 1341 516 Fragments arranged by size: 5040 1341 763 516 BclI T'GATC_A Cuts at: 3891 5178 3891 Size: 1287 6373 BfrBI ATG'CAT Cuts at: 2110 2110 Size: 7660 BglI GCCn_nnn'nGGC Cuts at: 3505 3505 Size: 7660 BglII A'GATC_T Cuts at: 2918 5173 7212 2918 Size: 2255 2039 3366 Fragments arranged by size: 3366 2255 2039 BlpI GC'TnA_GC Cuts at: 2807 4022 4770 2807 Size: 1215 748 5697 Fragments arranged by size: 5697 1215 748 Bme1580I G_kGCm'C Cuts at: 1057 1548 1697 3479 3510 3637 4332 5283 5376 Size: 491 149 1782 31 127 695 951 93 Cuts at: 5376 6434 1057 Size: 1058 2283 Fragments arranged by size: 2283 1782 1058 951 695 491 149 127 93 31 BmgBI CAC'GTC Cuts at: 4200 4200 Size: 7660 BmrI ACTGGGnnnn_n' Cuts at: 1683 4870 4951 5286 7432 1683 Size: 3187 81 335 2146 1911 Fragments arranged by size: 3187 2146 1911 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6025 6025 Size: 7660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1376 2199 3645 3659 1376 Size: 823 1446 14 5377 Fragments arranged by size: 5377 1446 823 14 Bpu10I CC'TnA_GC Cuts at: 4218 4586 4218 Size: 368 7292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 215 1067 3373 4354 4834 5713 6207 6505 215 Size: 852 2306 981 480 879 494 298 1370 Fragments arranged by size: 2306 1370 981 879 852 494 480 298 BsaI GGTCTCn'nnnn_ Cuts at: 3542 7364 3542 Size: 3822 3838 BsaAI yAC'GTr Cuts at: 3177 4650 5011 3177 Size: 1473 361 5826 Fragments arranged by size: 5826 1473 361 BsaBI GATnn'nnATC Cuts at: 6 757 5172 7211 6 Size: 751 4415 2039 455 Fragments arranged by size: 4415 2039 751 455 BsaHI Gr'CG_yC Cuts at: 3656 5089 5337 6041 7537 3656 Size: 1433 248 704 1496 3779 Fragments arranged by size: 3779 1496 1433 704 248 BsaWI w'CCGG_w Cuts at: 2484 4847 5368 6048 6322 6469 6880 2484 Size: 2363 521 680 274 147 411 3264 Fragments arranged by size: 3264 2363 680 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 205 235 1349 1379 3632 3662 205 Size: 30 1114 30 2253 30 4203 Fragments arranged by size: 4203 2253 1114 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 469 1143 3031 3407 3784 3805 4028 469 Size: 674 1888 376 377 21 223 4101 Fragments arranged by size: 4101 1888 674 377 376 223 21 BseYI C'CCAG_C Cuts at: 2069 2136 3502 3524 3852 4486 5083 5841 6420 Size: 67 1366 22 328 634 597 758 579 Cuts at: 6420 6916 2069 Size: 496 2813 Fragments arranged by size: 2813 1366 758 634 597 579 496 328 67 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 276 333 1322 4384 276 Size: 57 989 3062 3552 Fragments arranged by size: 3552 3062 989 57 BsiEI CG_ry'CG Cuts at: 104 5200 5246 6456 104 Size: 5096 46 1210 1308 Fragments arranged by size: 5096 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 1057 1697 2446 3479 3776 4265 4332 4672 5450 Size: 640 749 1033 297 489 67 340 778 Cuts at: 5450 5640 6434 1057 Size: 190 794 2283 Fragments arranged by size: 2283 1033 794 778 749 640 489 340 297 190 67 BsmBI CGTCTCn'nnnn_ Cuts at: 3663 3663 Size: 7660 Bsp1286I G_dGCh'C Cuts at: 1057 1548 1697 2446 3479 3510 3637 3776 4219 Size: 491 149 749 1033 31 127 139 443 Cuts at: 4219 4265 4332 4672 5283 5376 5450 5640 5702 Size: 46 67 340 611 93 74 190 62 Cuts at: 5702 6434 1057 Size: 732 2283 Fragments arranged by size: 2283 1033 749 732 611 491 443 340 190 149 139 127 93 74 67 62 46 31 BspEI T'CCGG_A Cuts at: 2484 6880 2484 Size: 4396 3264 BspHI T'CATG_A Cuts at: 1124 2454 6836 1124 Size: 1330 4382 1948 Fragments arranged by size: 4382 1948 1330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2942 3171 3227 3282 4708 4922 5224 5605 2942 Size: 229 56 55 1426 214 302 381 4997 Fragments arranged by size: 4997 1426 381 302 229 214 56 55 BsrBI CCG'CTC Cuts at: 50 5950 6004 7490 7655 50 Size: 5900 54 1486 165 55 Fragments arranged by size: 5900 1486 165 55 54 BsrDI GCAATG_nn' Cuts at: 3494 5570 3494 Size: 2076 5584 BsrFI r'CCGG_y Cuts at: 3752 3782 5656 5837 6048 3752 Size: 30 1874 181 211 5364 Fragments arranged by size: 5364 1874 211 181 30 BsrGI T'GTAC_A Cuts at: 1251 2640 3395 1251 Size: 1389 755 5516 Fragments arranged by size: 5516 1389 755 BssSI C'ACGA_G Cuts at: 5929 6289 5929 Size: 360 7300 BstAPI GCAn_nnn'nTGC Cuts at: 3474 5035 3474 Size: 1561 6099 BstBI TT'CG_AA Cuts at: 110 110 Size: 7660 BstXI CCAn_nnnn'nTGG Cuts at: 3315 3735 3315 Size: 420 7240 BstYI r'GATC_y Cuts at: 1 215 1373 2918 4683 5150 5173 5508 5754 Size: 214 1158 1545 1765 467 23 335 246 Cuts at: 5754 6768 6854 6866 7212 1 Size: 1014 86 12 346 449 Fragments arranged by size: 1765 1545 1158 1014 467 449 346 335 246 214 86 23 12 BstZ17I GTA'TAC Cuts at: 1879 3195 1879 Size: 1316 6344 Bsu36I CC'TnA_GG Cuts at: 446 4630 446 Size: 4184 3476 BtgI C'CryG_G Cuts at: 1862 4610 1862 Size: 2748 4912 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 338 5608 5789 5952 338 Size: 5270 181 163 2046 Fragments arranged by size: 5270 2046 181 163 BtsI GCAGTG_nn' Cuts at: 410 502 1225 2777 3458 4901 410 Size: 92 723 1552 681 1443 3169 Fragments arranged by size: 3169 1552 1443 723 681 92 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 3660 3695 3660 Size: 35 7625 DraI TTT'AAA Cuts at: 2742 4657 2742 Size: 1915 5745 DrdI GACnn_nn'nnGTC Cuts at: 5364 6224 5364 Size: 860 6800 EaeI y'GGCC_r Cuts at: 513 5243 5417 5808 5835 7182 513 Size: 4730 174 391 27 1347 991 Fragments arranged by size: 4730 1347 991 391 174 27 EagI C'GGCC_G Cuts at: 5243 5243 Size: 7660 EarI CTCTTCn'nnn_ Cuts at: 2873 4270 5681 5891 2873 Size: 1397 1411 210 4642 Fragments arranged by size: 4642 1411 1397 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5866 6178 6324 7470 5866 Size: 312 146 1146 6056 Fragments arranged by size: 6056 1146 312 146 EcoICRI GAG'CTC Cuts at: 4670 4670 Size: 7660 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 144 276 450 1277 1376 2104 2199 3588 3645 Size: 132 174 827 99 728 95 1389 57 Cuts at: 3645 3659 3920 4144 5483 5915 6664 6932 144 Size: 14 261 224 1339 432 749 268 872 Fragments arranged by size: 1389 1339 872 827 749 728 432 268 261 224 174 132 99 95 57 14 EcoNI CCTnn'n_nnAGG Cuts at: 2153 3823 2153 Size: 1670 5990 EcoO109I rG'GnC_Cy Cuts at: 1545 1572 2692 2931 3311 3445 3506 3507 3633 Size: 27 1120 239 380 134 61 1 126 Cuts at: 3633 3634 3840 4797 1545 Size: 1 206 957 4408 Fragments arranged by size: 4408 1120 957 380 239 206 134 126 61 27 1 1 EcoRI G'AATT_C Cuts at: 1384 4662 1384 Size: 3278 4382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2524 5677 2524 Size: 3153 4507 FspI TGC'GCA Cuts at: 5439 5439 Size: 7660 HaeII r_GCGC'y Cuts at: 579 2722 4092 5092 5340 6364 7343 579 Size: 2143 1370 1000 248 1024 979 896 Fragments arranged by size: 2143 1370 1024 1000 979 896 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 761 793 1119 1151 2333 2365 3434 3466 3742 Size: 32 326 32 1182 32 1069 32 276 Cuts at: 3742 3774 4015 4047 4166 4198 4408 4440 4458 Size: 32 241 32 119 32 210 32 18 Cuts at: 4458 4490 5500 5530 5532 5562 761 Size: 32 1010 30 2 30 2859 Fragments arranged by size: 2859 1182 1069 1010 326 276 241 210 119 32 32 32 32 32 32 32 32 32 30 30 18 2 HincII GTy'rAC Cuts at: 4697 4697 Size: 7660 HindIII A'AGCT_T Cuts at: 422 1652 422 Size: 1230 6430 KasI G'GCGC_C Cuts at: 5088 5336 5088 Size: 248 7412 KpnI G_GTAC'C Cuts at: 3704 4678 3704 Size: 974 6686 MfeI C'AATT_G Cuts at: 3875 3875 Size: 7660 MluI A'CGCG_T Cuts at: 7093 7093 Size: 7660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3041 3074 6331 6515 3041 Size: 33 3257 184 4186 Fragments arranged by size: 4186 3257 184 33 MscI TGG'CCA Cuts at: 515 5419 515 Size: 4904 2756 MslI CAynn'nnrTG Cuts at: 1015 2648 2668 3991 5647 5774 1015 Size: 1633 20 1323 1656 127 2901 Fragments arranged by size: 2901 1656 1633 1323 127 20 MspA1I CmG'CkG Cuts at: 572 597 2806 3502 4490 4769 5083 5443 6458 Size: 25 2209 696 988 279 314 360 1015 Cuts at: 6458 6703 572 Size: 245 1529 Fragments arranged by size: 2209 1529 1015 988 696 360 314 279 245 25 NaeI GCC'GGC Cuts at: 5839 5839 Size: 7660 NarI GG'CG_CC Cuts at: 5089 5337 5089 Size: 248 7412 NcoI C'CATG_G Cuts at: 1862 4610 1862 Size: 2748 4912 NdeI CA'TA_TG Cuts at: 1417 1509 1417 Size: 92 7568 NgoMIV G'CCGG_C Cuts at: 5837 5837 Size: 7660 NruI TCG'CGA Cuts at: 6021 6021 Size: 7660 NsiI A_TGCA'T Cuts at: 2112 2112 Size: 7660 NspI r_CATG'y Cuts at: 177 394 4114 4711 5211 6120 177 Size: 217 3720 597 500 909 1717 Fragments arranged by size: 3720 1717 909 597 500 217 PasI CC'CwG_GG Cuts at: 1541 2872 1541 Size: 1331 6329 PciI A'CATG_T Cuts at: 173 6116 173 Size: 5943 1717 PflMI CCAn_nnn'nTGG Cuts at: 4566 4566 Size: 7660 PfoI T'CCnGG_A Cuts at: 1203 1824 2246 1203 Size: 621 422 6617 Fragments arranged by size: 6617 621 422 PmeI GTTT'AAAC Cuts at: 4657 4657 Size: 7660 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 205 237 693 725 6799 6831 205 Size: 32 456 32 6074 32 1034 Fragments arranged by size: 6074 1034 456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 1572 2692 2931 3445 1572 Size: 1120 239 514 5787 Fragments arranged by size: 5787 1120 514 239 PshAI GACnn'nnGTC Cuts at: 2090 2333 2090 Size: 243 7417 PsiI TTA'TAA Cuts at: 1943 2380 2957 1943 Size: 437 577 6646 Fragments arranged by size: 6646 577 437 PspOMI G'GGCC_C Cuts at: 1544 3506 3633 1544 Size: 1962 127 5571 Fragments arranged by size: 5571 1962 127 PspXI vC'TCGA_Gb Cuts at: 2339 2339 Size: 7660 PstI C_TGCA'G Cuts at: 2939 4530 4705 5390 2939 Size: 1591 175 685 5209 Fragments arranged by size: 5209 1591 685 175 PvuI CG_AT'CG Cuts at: 104 104 Size: 7660 PvuII CAG'CTG Cuts at: 572 597 2806 4490 5083 5443 572 Size: 25 2209 1684 593 360 2789 Fragments arranged by size: 2789 2209 1684 593 360 25 RsrII CG'GwC_CG Cuts at: 5853 5853 Size: 7660 SacI G_AGCT'C Cuts at: 4672 4672 Size: 7660 SalI G'TCGA_C Cuts at: 4695 4695 Size: 7660 SapI GCTCTTCn'nnn_ Cuts at: 2873 4270 5681 5891 2873 Size: 1397 1411 210 4642 Fragments arranged by size: 4642 1411 1397 210 SbfI CC_TGCA'GG Cuts at: 4705 4705 Size: 7660 ScaI AGT'ACT Cuts at: 2102 7177 2102 Size: 5075 2585 SexAI A'CCwGG_T Cuts at: 4565 4565 Size: 7660 SfcI C'TryA_G Cuts at: 33 780 848 1606 2192 2273 2935 4526 4701 Size: 747 68 758 586 81 662 1591 175 Cuts at: 4701 5386 6381 6572 33 Size: 685 995 191 1121 Fragments arranged by size: 1591 1121 995 758 747 685 662 586 191 175 81 68 SfoI GGC'GCC Cuts at: 5090 5338 5090 Size: 248 7412 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7660 SmaI CCC'GGG Cuts at: 2855 3631 4680 2855 Size: 776 1049 5835 Fragments arranged by size: 5835 1049 776 SmlI C'TyrA_G Cuts at: 230 1082 2339 3388 4369 4813 5728 6222 6484 Size: 852 1257 1049 981 444 915 494 262 Cuts at: 6484 230 Size: 1406 Fragments arranged by size: 1406 1257 1049 981 915 852 494 444 262 SnaBI TAC'GTA Cuts at: 4650 4650 Size: 7660 SpeI A'CTAG_T Cuts at: 67 67 Size: 7660 SphI G_CATG'C Cuts at: 4711 5211 4711 Size: 500 7160 SspI AAT'ATT Cuts at: 537 1937 537 Size: 1400 6260 StuI AGG'CCT Cuts at: 4303 4303 Size: 7660 StyI C'CwwG_G Cuts at: 381 1862 3314 3953 4610 4792 5146 381 Size: 1481 1452 639 657 182 354 2895 Fragments arranged by size: 2895 1481 1452 657 639 354 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2803 5241 5374 5833 6044 2803 Size: 2438 133 459 211 4419 Fragments arranged by size: 4419 2438 459 211 133 TatI w'GTAC_w Cuts at: 566 689 741 998 1251 2100 2640 3395 4436 Size: 123 52 257 253 849 540 755 1041 Cuts at: 4436 7175 566 Size: 2739 1051 Fragments arranged by size: 2739 1051 1041 849 755 540 257 253 123 52 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3210 3242 3632 3664 7177 7209 3210 Size: 32 390 32 3513 32 3661 Fragments arranged by size: 3661 3513 390 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5455 5455 Size: 7660 XbaI T'CTAG_A Cuts at: 249 2538 4689 249 Size: 2289 2151 3220 Fragments arranged by size: 3220 2289 2151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 327 2220 3057 5153 327 Size: 1893 837 2096 2834 Fragments arranged by size: 2834 2096 1893 837 XhoI C'TCGA_G Cuts at: 2339 2339 Size: 7660 XmaI C'CCGG_G Cuts at: 2853 3629 4678 2853 Size: 776 1049 5835 Fragments arranged by size: 5835 1049 776 XmnI GAAnn'nnTTC Cuts at: 289 536 705 1655 3014 289 Size: 247 169 950 1359 4935 Fragments arranged by size: 4935 1359 950 247 169 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AscI AvrII BbvCI BcgI BmtI BsiWI BsmI BssHII BstEII ClaI DraIII EcoRV FseI FspAI HpaI NheI NotI PacI PmlI PsrI SacII SanDI SfiI SgrAI SrfI SwaI ZraI