Restriction map for pF1KSDA0525
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AflIII      AgeI        AscI        AseI        AsnI        
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      CpoI        
DrdI        EaeI        EagI        EclXI       Eco52I      
EcoO65I     EcoRI       FauI        FbaI        FseI        
FspI        HpaI        KspI        MamI        MluI        
MluNI       MroI        MscI        MspA1I      MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1679  GGTACC               G^GTACC
AcyI         1663  GGCGCC               G[GA]^CG[CT]C
AhdI          937  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2209  GTGCAC               G^TGCAC
Aor51HI      1357  AGCGCT               AGC^GCT
ApaI         2324  GGGCCC               GGGCC^C
ApaLI        2209  GTGCAC               G^TGCAC
Asp700I      2434  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1679  GGTACC               G^GTACC
AspEI         937  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          406  CCCGAG               C^[CT]CG[GA]G
BbeI         1663  GGCGCC               GGCGC^C
BglII         918  AGATCT               A^GATCT
BsaHI        1663  GGCGCC               G[GA]^CG[CT]C
BseRI        2197  CTCCTC               GAGGAG(10/8)
BsoBI         406  CCCGAG               C^[CT]CG[GA]G
BspDI        1733  ATCGAT               AT^CGAT
BsrDI        1219  GCAATG               GCAATG(2/0)
ClaI         1733  ATCGAT               AT^CGAT
DraIII       1249  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          477  CCACGG               C^C[GA][CT]GG
Eam1105I      937  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1357  AGCGCT               AGC^GCT
EcoNI         318  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         303  GATATC               GAT^ATC
EcoT22I      1925  ATGCAT               ATGCA^T
Hin1I        1663  GGCGCC               G[GA]^CG[CT]C
KasI         1663  GGCGCC               G^GCGCC
KpnI         1679  GGTACC               GGTAC^C
MfeI         2732  CAATTG               C^AATTG
MunI         2732  CAATTG               C^AATTG
NarI         1663  GGCGCC               GG^CGCC
NsiI         1925  ATGCAT               ATGCA^T
NspI         1689  GCATGT               [GA]CATG^[CT]
PflMI        1056  CCATGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI         2293  GTCGAC               G^TCGAC
SbfI          357  CCTGCAGG             CCTGCA^GG
ScaI         2383  AGTACT               AGT^ACT
SexAI        1085  ACCTGGT              A^CC[AT]GGT
SfoI         1663  GGCGCC               GGC^GCC
SmlI         2280  CTTGAG               C^T[CT][GA]AG
Sse8387I      357  CCTGCAGG             CCTGCA^GG
Van91I       1056  CCATGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2434  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1257  GTCTAC               GT^[AC][GT]AC
             2293  GTCGAC              
AciI          856  GCGG                 CCGC(-3/-1)
             2181  GCGG                
AlwNI         361  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1043  CAGTGACTG           
AocI          318  CCTCAGG              CC^T[ACGT]AGG
              379  CCTCAGG             
BbsI          697  GAAGAC               GAAGAC(2/6)
             1014  GTCTTC              
BcnI         1287  CCGGG                CC^[GC]GG
             1668  CCCGG               
BpmI         1393  CTCCAG               CTGGAG(16/14)
              332  CTGGAG              
BpuAI         697  GAAGAC               GAAGAC(2/6)
             1014  GTCTTC              
BstYI         918  AGATCT               [GA]^GATC[CT]
             1283  AGATCC              
Bsu36I        318  CCTCAGG              CC^T[ACGT]AGG
              379  CCTCAGG             
CfoI         1358  GCGC                 GCG^C
             1664  GCGC                
Eco81I        318  CCTCAGG              CC^T[ACGT]AGG
              379  CCTCAGG             
HaeII        1357  AGCGCT               [GA]GCGC^[CT]
             1663  GGCGCC              
HapII        1287  CCGG                 C^CGG
             1669  CCGG                
HhaI         1358  GCGC                 GCG^C
             1664  GCGC                
HinP1I       1358  GCGC                 G^CGC
             1664  GCGC                
HincII       1502  GTCAAC               GT[CT]^[GA]AC
             2293  GTCGAC              
HindII       1502  GTCAAC               GT[CT]^[GA]AC
             2293  GTCGAC              
HpaII        1287  CCGG                 C^CGG
             1669  CCGG                
MflI          918  AGATCT               [GA]^GATC[CT]
             1283  AGATCC              
MspI         1287  CCGG                 C^CGG
             1669  CCGG                
NciI         1287  CCGGG                CC^[GC]GG
             1668  CCCGG               
StuI         1967  AGGCCT               AGG^CCT
             2099  AGGCCT              
TaqI         1734  TCGA                 T^CGA
             2294  TCGA                
TthHB8I      1734  TCGA                 T^CGA
             2294  TCGA                
XbaI          867  TCTAGA               T^CTAGA
             1495  TCTAGA              
XhoII         918  AGATCT               [GA]^GATC[CT]
             1283  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       AvaII       BanI        BanII       BbvI        
BciVI       BfaI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HgaI        HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XcmI        
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