Restriction map for pF1KSDA0525
 (Linear) (Six-base) MAPSORT of: KIAA0525.seq  Check: 9102  from: 1  to: 2844



KIAA0525.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     305    2844 
   Size:        305    2539
 
AccI GT'mk_AC

Cuts at:      0    1258    2294    2844 
   Size:       1258    1036     550

  Fragments arranged by size: 

                1258    1036     550
 
Acc65I G'GTAC_C

Cuts at:      0    1679    2844 
   Size:       1679    1165
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     704    2667    2844 
   Size:        125     579    1963     177

  Fragments arranged by size: 

                1963     579     177     125
 
AfeI AGC'GCT

Cuts at:      0    1359    2844 
   Size:       1359    1485
 
AhdI GACnn_n'nnGTC

Cuts at:      0     942    2844 
   Size:        942    1902
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1470    2844 
   Size:       1470    1374
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2180    2212    2844 
   Size:       2180      32     632

  Fragments arranged by size: 

                2180     632      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366    1048    2844 
   Size:        366     682    1796

  Fragments arranged by size: 

                1796     682     366
 
ApaI G_GGCC'C

Cuts at:      0    2328    2844 
   Size:       2328     516
 
ApaLI G'TGCA_C

Cuts at:      0    2209    2844 
   Size:       2209     635
 
ApoI r'AATT_y

Cuts at:      0     871    1138    1239    1795    2404    2501    2772    2844 
   Size:        871     267     101     556     609      97     271      72

  Fragments arranged by size: 

                 871     609     556     271     267     101      97      72
            
 
AvaI C'yCGr_G

Cuts at:      0     406    2844 
   Size:        406    2438
 
BanI G'GyrC_C

Cuts at:      0     635    1663    1679    2844 
   Size:        635    1028      16    1165

  Fragments arranged by size: 

                1165    1028     635      16
 
BanII G_rGCy'C

Cuts at:      0     413     428    1657    2161    2328    2844 
   Size:        413      15    1229     504     167     516

  Fragments arranged by size: 

                1229     516     504     413     167      15
 
BbeI G_GCGC'C

Cuts at:      0    1667    2844 
   Size:       1667    1177
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     704    1007    2844 
   Size:        704     303    1837

  Fragments arranged by size: 

                1837     704     303
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     518     911    2527    2844 
   Size:        518     393    1616     317

  Fragments arranged by size: 

                1616     518     393     317
 
BfrBI ATG'CAT

Cuts at:      0    1927    2844 
   Size:       1927     917
 
BglII A'GATC_T

Cuts at:      0     918    2844 
   Size:        918    1926
 
Bme1580I G_kGCm'C

Cuts at:      0    2213    2328    2844 
   Size:       2213     115     516

  Fragments arranged by size: 

                2213     516     115
 
BmgBI CAC'GTC

Cuts at:      0    2298    2844 
   Size:       2298     546
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172     972    1685    2521    2844 
   Size:        172     800     713     836     323

  Fragments arranged by size: 

                 836     800     713     323     172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353    1378    2844 
   Size:        353    1025    1466

  Fragments arranged by size: 

                1466    1025     353
 
Bpu10I CC'TnA_GC

Cuts at:      0    1342    2119    2844 
   Size:       1342     777     725

  Fragments arranged by size: 

                1342     777     725
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2301    2844 
   Size:       2301     543
 
BsaHI Gr'CG_yC

Cuts at:      0    1664    2844 
   Size:       1664    1180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     782     812    1138    1168    1376    1406    2180    2210 
   Size:        782      30     326      30     208      30     774      30

Cuts at:   2210    2844 
   Size:        634

  Fragments arranged by size: 

                 782     774     634     326     208      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2188    2844 
   Size:       2188     656
 
BseYI C'CCAG_C

Cuts at:      0    2342    2844 
   Size:       2342     502
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     730    1643    1766    2213    2335    2844 
   Size:        103     627     913     123     447     122     509

  Fragments arranged by size: 

                 913     627     509     447     123     122     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     413     428     730    1643    1657    1766    2161 
   Size:        103     310      15     302     913      14     109     395

Cuts at:   2161    2213    2328    2335    2844 
   Size:         52     115       7     509

  Fragments arranged by size: 

                 913     509     395     310     302     115     109     103
                  52      15      14       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     305     351     597    2844 
   Size:        305      46     246    2247

  Fragments arranged by size: 

                2247     305     246      46
 
BsrDI GCAATG_nn'

Cuts at:      0    1226    2844 
   Size:       1226    1618
 
BsrGI T'GTAC_A

Cuts at:      0    1383    1623    2580    2844 
   Size:       1383     240     957     264

  Fragments arranged by size: 

                1383     957     264     240
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     841     850    2178    2314    2844 
   Size:        841       9    1328     136     530

  Fragments arranged by size: 

                1328     841     530     136       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     143    1078    1143    2546    2844 
   Size:        143     935      65    1403     298

  Fragments arranged by size: 

                1403     935     298     143      65
 
BstYI r'GATC_y

Cuts at:      0     918    1283    2844 
   Size:        918     365    1561

  Fragments arranged by size: 

                1561     918     365
 
Bsu36I CC'TnA_GG

Cuts at:      0     319     380    2844 
   Size:        319      61    2464

  Fragments arranged by size: 

                2464     319      61
 
BtgI C'CryG_G

Cuts at:      0     477    2844 
   Size:        477    2367
 
BtsI GCAGTG_nn'

Cuts at:      0     394     551    1573    2315    2844 
   Size:        394     157    1022     742     529

  Fragments arranged by size: 

                1022     742     529     394     157
 
ClaI AT'CG_AT

Cuts at:      0    1734    2844 
   Size:       1734    1110
 
DraI TTT'AAA

Cuts at:      0    1238    1371    1871    2844 
   Size:       1238     133     500     973

  Fragments arranged by size: 

                1238     973     500     133
 
DraIII CAC_nnn'GTG

Cuts at:      0    1254    2844 
   Size:       1254    1590
 
EarI CTCTTCn'nnn_

Cuts at:      0     618     869    1772    2660    2844 
   Size:        618     251     903     888     184

  Fragments arranged by size: 

                 903     888     618     251     184
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     353     704    1378    2667    2844 
   Size:        125     228     351     674    1289     177

  Fragments arranged by size: 

                1289     674     351     228     177     125
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     322    2844 
   Size:        322    2522
 
EcoO109I rG'GnC_Cy

Cuts at:      0     363    1428    2124    2324    2844 
   Size:        363    1065     696     200     520

  Fragments arranged by size: 

                1065     696     520     363     200
 
EcoRV GAT'ATC

Cuts at:      0     305    2844 
   Size:        305    2539
 
HaeII r_GCGC'y

Cuts at:      0    1361    1667    2844 
   Size:       1361     306    1177

  Fragments arranged by size: 

                1361    1177     306
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     328     360     717     748     749     780     928     960 
   Size:        328      32     357      31       1      31     148      32

Cuts at:    960    1138    1170    2143    2175    2844 
   Size:        178      32     973      32     669

  Fragments arranged by size: 

                 973     669     357     328     178     148      32      32
                  32      32      31      31       1
 
HincII GTy'rAC

Cuts at:      0    1504    2295    2844 
   Size:       1504     791     549

  Fragments arranged by size: 

                1504     791     549
 
HindIII A'AGCT_T

Cuts at:      0    1030    2437    2460    2809    2844 
   Size:       1030    1407      23     349      35

  Fragments arranged by size: 

                1407    1030     349      35      23
 
KasI G'GCGC_C

Cuts at:      0    1663    2844 
   Size:       1663    1181
 
KpnI G_GTAC'C

Cuts at:      0    1683    2844 
   Size:       1683    1161
 
MfeI C'AATT_G

Cuts at:      0    2732    2844 
   Size:       2732     112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1167    1745    2740    2748    2844 
   Size:       1167     578     995       8      96

  Fragments arranged by size: 

                1167     995     578      96       8
 
MslI CAynn'nnrTG

Cuts at:      0     707    1042    1606    2623    2844 
   Size:        707     335     564    1017     221

  Fragments arranged by size: 

                1017     707     564     335     221
 
NarI GG'CG_CC

Cuts at:      0    1664    2844 
   Size:       1664    1180
 
NsiI A_TGCA'T

Cuts at:      0    1929    2844 
   Size:       1929     915
 
NspI r_CATG'y

Cuts at:      0    1693    2844 
   Size:       1693    1151
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1062    2844 
   Size:       1062    1782
 
PfoI T'CCnGG_A

Cuts at:      0     365    1286    2844 
   Size:        365     921    1558

  Fragments arranged by size: 

                1558     921     365
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2180    2212    2844 
   Size:       2180      32     632

  Fragments arranged by size: 

                2180     632      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     363    1428    2124    2844 
   Size:        363    1065     696     720

  Fragments arranged by size: 

                1065     720     696     363
 
PspOMI G'GGCC_C

Cuts at:      0    2324    2844 
   Size:       2324     520
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     509     541    2844 
   Size:        509      32    2303

  Fragments arranged by size: 

                2303     509      32
 
PstI C_TGCA'G

Cuts at:      0     302     362     558    1580    2419    2844 
   Size:        302      60     196    1022     839     425

  Fragments arranged by size: 

                1022     839     425     302     196      60
 
SalI G'TCGA_C

Cuts at:      0    2293    2844 
   Size:       2293     551
 
SbfI CC_TGCA'GG

Cuts at:      0     362    2844 
   Size:        362    2482
 
ScaI AGT'ACT

Cuts at:      0    2385    2844 
   Size:       2385     459
 
SexAI A'CCwGG_T

Cuts at:      0    1085    2844 
   Size:       1085    1759
 
SfcI C'TryA_G

Cuts at:      0     298     358     498     554    1460    1576    2415    2844 
   Size:        298      60     140      56     906     116     839     429

  Fragments arranged by size: 

                 906     839     429     298     140     116      60      56
            
 
SfoI GGC'GCC

Cuts at:      0    1665    2844 
   Size:       1665    1179
 
SmlI C'TyrA_G

Cuts at:      0    2280    2844 
   Size:       2280     564
 
StuI AGG'CCT

Cuts at:      0    1969    2101    2844 
   Size:       1969     132     743

  Fragments arranged by size: 

                1969     743     132
 
StyI C'CwwG_G

Cuts at:      0     142     506     631    1970    2303    2844 
   Size:        142     364     125    1339     333     541

  Fragments arranged by size: 

                1339     541     364     333     142     125
 
TatI w'GTAC_w

Cuts at:      0    1383    1623    1985    2383    2580    2844 
   Size:       1383     240     362     398     197     264

  Fragments arranged by size: 

                1383     398     362     264     240     197
 
XbaI T'CTAG_A

Cuts at:      0     867    1495    2844 
   Size:        867     628    1349

  Fragments arranged by size: 

                1349     867     628
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     657    1075    2616    2844 
   Size:        657     418    1541     228

  Fragments arranged by size: 

                1541     657     418     228
 
XmnI GAAnn'nnTTC

Cuts at:      0    2438    2844 
   Size:       2438     406
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AhdI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BciVI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     DraI   DraIII     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI
     MmeI     MslI     NarI     NsiI     NspI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PsrI     PstI     SalI     SbfI     ScaI    SexAI
     SfcI     SfoI     SmlI     StuI     StyI     TatI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AleI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI     BclI     BglI
     BlpI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI    CspCI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI     HpaI     MluI     MscI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I
     XhoI     XmaI     ZraI
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