Restriction map for pF1KSDA0517
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AspEI       AspI        
AvrII       BbeI        BbrPI       BcgI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspEI       
BspHI       BspLU11I    BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        HaeII       Hin1I       HindIII     KasI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1510  GGTACC               G^GTACC
Alw44I        501  GTGCAC               G^TGCAC
ApaLI         501  GTGCAC               G^TGCAC
AseI         1581  ATTAAT               AT^TAAT
AsnI         1581  ATTAAT               AT^TAAT
Asp718I      1510  GGTACC               G^GTACC
AviII         731  TGCGCA               TGC^GCA
BamHI        2156  GGATCC               G^GATCC
BanII         709  GAGCTC               G[GA]GC[CT]^C
BbvCI         373  GCTGAGG              CCTCAGC(-5/-2)
BciVI         804  GGATAC               GTATCC(6/5)
BfaI           77  CTAG                 C^TAG
BglI         2225  GCCCCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          880  GAGACC               GGTCTC(1/5)
BspDI        2300  ATCGAT               AT^CGAT
BspMI        1948  GCAGGT               ACCTGC(4/8)
BsrBI        1407  GAGCGG               CCGCTC(-3/-3)
BstAPI        149  GCAGTATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         2300  ATCGAT               AT^CGAT
DraI          723  TTTAAA               TTT^AAA
EcoRI        2041  GAATTC               G^AATTC
FspI          731  TGCGCA               TGC^GCA
HgaI         1661  GACGC                GACGC(5/10)
HpaI          657  GTTAAC               GTT^AAC
KpnI         1510  GGTACC               GGTAC^C
KspI          958  CCGCGG               CCGC^GG
MaeI           77  CTAG                 C^TAG
NaeI           55  GCCGGC               GCC^GGC
NcoI          870  CCATGG               C^CATGG
NgoMIV         55  GCCGGC               G^CCGGC
NspI         1448  GCATGT               [GA]CATG^[CT]
PshAI         882  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1581  ATTAAT               AT^TAAT
SacI          709  GAGCTC               GAGCT^C
SacII         958  CCGCGG               CCGC^GG
SapI          625  GCTCTTC              GCTCTTC(1/4)
SexAI        1909  ACCAGGT              A^CC[AT]GGT
SfiI         2224  GGCCCCCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1401  CCCGGG               CCC^GGG
SmlI          541  CTCAAG               C^T[CT][GA]AG
XcmI         2160  CCAGGTTTTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1401  CCCGGG               C^CCGGG
XmaI         1401  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

Asp700I       315  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              643  GAAATCATTC          
BseRI        1761  CTCCTC               GAGGAG(10/8)
              376  GAGGAG              
BsrDI        1725  CATTGC               GCAATG(2/0)
             1835  GCAATG              
BsrFI          55  GCCGGC               [GA]^CCGG[CT]
             1159  ACCGGC              
BstYI        1220  AGATCC               [GA]^GATC[CT]
             2156  GGATCC              
Cfr10I         55  GCCGGC               [GA]^CCGG[CT]
             1159  ACCGGC              
EarI          626  CTCTTC               CTCTTC(1/4)
             1383  GAAGAG              
Ksp632I       626  CTCTTC               CTCTTC(1/4)
             1383  GAAGAG              
MaeII         344  ACGT                 A^CGT
             1415  ACGT                
MflI         1220  AGATCC               [GA]^GATC[CT]
             2156  GGATCC              
PflMI        2215  CCACTCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2229  CCAAGGCCTGG         
StuI          566  AGGCCT               AGG^CCT
             2232  AGGCCT              
Van91I       2215  CCACTCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2229  CCAAGGCCTGG         
XhoII        1220  AGATCC               [GA]^GATC[CT]
             2156  GGATCC              
XmnI          315  GAACAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              643  GAAATCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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