Restriction map for pF1KSDA0517
 (Linear) (Six-base) MAPSORT of: KIAA0517.seq  Check: 8180  from: 1  to: 2313



KIAA0517.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

Acc65I G'GTAC_C

Cuts at:      0    1510    2313 
   Size:       1510     803
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613    1024    1531    2231    2313 
   Size:        613     411     507     700      82

  Fragments arranged by size: 

                 700     613     507     411      82
 
AleI CACnn'nnGTG

Cuts at:      0       8     406    1693    2313 
   Size:          8     398    1287     620

  Fragments arranged by size: 

                1287     620     398       8
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      53     757    2105    2313 
   Size:         53     704    1348     208

  Fragments arranged by size: 

                1348     704     208      53
 
AlwNI CAG_nnn'CTG

Cuts at:      0     141     403    2275    2313 
   Size:        141     262    1872      38

  Fragments arranged by size: 

                1872     262     141      38
 
ApaLI G'TGCA_C

Cuts at:      0     501    2313 
   Size:        501    1812
 
ApoI r'AATT_y

Cuts at:      0     460    1774    2041    2313 
   Size:        460    1314     267     272

  Fragments arranged by size: 

                1314     460     272     267
 
AseI AT'TA_AT

Cuts at:      0    1582    2313 
   Size:       1582     731
 
AvaI C'yCGr_G

Cuts at:      0     289    1024    1401    2313 
   Size:        289     735     377     912

  Fragments arranged by size: 

                 912     735     377     289
 
BamHI G'GATC_C

Cuts at:      0    2156    2313 
   Size:       2156     157
 
BanI G'GyrC_C

Cuts at:      0      94     215    1240    1510    2313 
   Size:         94     121    1025     270     803

  Fragments arranged by size: 

                1025     803     270     121      94
 
BanII G_rGCy'C

Cuts at:      0     713    2313 
   Size:        713    1600
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     374     724    1493    2313 
   Size:        374     350     769     820

  Fragments arranged by size: 

                 820     769     374     350
 
BbvCI CC'TCA_GC

Cuts at:      0     374    2313 
   Size:        374    1939
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     798    2313 
   Size:        798    1515
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2231    2313 
   Size:       2231      82
 
Bme1580I G_kGCm'C

Cuts at:      0     505    1844    2313 
   Size:        505    1339     469

  Fragments arranged by size: 

                1339     505     469
 
BmgBI CAC'GTC

Cuts at:      0     345    1416    2313 
   Size:        345    1071     897

  Fragments arranged by size: 

                1071     897     345
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     256    1703    2151    2313 
   Size:        256    1447     448     162

  Fragments arranged by size: 

                1447     448     256     162
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     344     412     559     697     775     799    2313 
   Size:        295      49      68     147     138      78      24    1514

  Fragments arranged by size: 

                1514     295     147     138      78      68      49      24
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     374    2313 
   Size:        374    1939
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526    2313 
   Size:        526    1787
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     874    2313 
   Size:        874    1439
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      30    1685    1715    1811    1841    2313 
   Size:         30    1655      30      96      30     472

  Fragments arranged by size: 

                1655     472      96      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391    1752    2313 
   Size:        391    1361     561

  Fragments arranged by size: 

                1361     561     391
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     181    1630    2313 
   Size:         59     122    1449     683

  Fragments arranged by size: 

                1449     683     122      59
 
BsiHKAI G_wGCw'C

Cuts at:      0     505     518     527     713    1192    2313 
   Size:        505      13       9     186     479    1121

  Fragments arranged by size: 

                1121     505     479     186      13       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     518     527     713    1192    1844    2313 
   Size:        505      13       9     186     479     652     469

  Fragments arranged by size: 

                 652     505     479     469     186      13       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1939    2313 
   Size:       1939     374
 
BsrBI CCG'CTC

Cuts at:      0    1409    2313 
   Size:       1409     904
 
BsrDI GCAATG_nn'

Cuts at:      0    1724    1842    2313 
   Size:       1724     118     471

  Fragments arranged by size: 

                1724     471     118
 
BsrFI r'CCGG_y

Cuts at:      0      55    1159    2313 
   Size:         55    1104    1154

  Fragments arranged by size: 

                1154    1104      55
 
BsrGI T'GTAC_A

Cuts at:      0    1256    1451    1701    2313 
   Size:       1256     195     250     612

  Fragments arranged by size: 

                1256     612     250     195
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     155    2313 
   Size:        155    2158
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      71     681    1695    2313 
   Size:         71     610    1014     618

  Fragments arranged by size: 

                1014     618     610      71
 
BstYI r'GATC_y

Cuts at:      0    1220    2156    2313 
   Size:       1220     936     157

  Fragments arranged by size: 

                1220     936     157
 
BtgI C'CryG_G

Cuts at:      0     870     958    1070    2313 
   Size:        870      88     112    1243

  Fragments arranged by size: 

                1243     870     112      88
 
BtsI GCAGTG_nn'

Cuts at:      0     401    2114    2313 
   Size:        401    1713     199

  Fragments arranged by size: 

                1713     401     199
 
ClaI AT'CG_AT

Cuts at:      0    2301    2313 
   Size:       2301      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      95     130    2313 
   Size:         95      35    2183

  Fragments arranged by size: 

                2183      95      35
 
DraI TTT'AAA

Cuts at:      0     725    2313 
   Size:        725    1588
 
EaeI y'GGCC_r

Cuts at:      0      12      83     302     932    1067    1410    1629    2135 
   Size:         12      71     219     630     135     343     219     506

Cuts at:   2135    2313 
   Size:        178

  Fragments arranged by size: 

                 630     506     343     219     219     178     135      71
                  12
 
EarI CTCTTCn'nnn_

Cuts at:      0     632    1378    2313 
   Size:        632     746     935

  Fragments arranged by size: 

                 935     746     632
 
EcoICRI GAG'CTC

Cuts at:      0     711    2313 
   Size:        711    1602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     344     412     559     613     697     775     799 
   Size:        295      49      68     147      54      84      78      24

Cuts at:    799    1024    1531    2231    2313 
   Size:        225     507     700      82

  Fragments arranged by size: 

                 700     507     295     225     147      84      82      78
                  68      54      49      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     861     888    1810    1951    2313 
   Size:        861      27     922     141     362

  Fragments arranged by size: 

                 922     861     362     141      27
 
EcoRI G'AATT_C

Cuts at:      0    2041    2313 
   Size:       2041     272
 
FspI TGC'GCA

Cuts at:      0     733    2313 
   Size:        733    1580
 
HincII GTy'rAC

Cuts at:      0     597     659     765    2313 
   Size:        597      62     106    1548

  Fragments arranged by size: 

                1548     597     106      62
 
HpaI GTT'AAC

Cuts at:      0     659    2313 
   Size:        659    1654
 
KpnI G_GTAC'C

Cuts at:      0    1514    2313 
   Size:       1514     799
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2119    2313 
   Size:       2119     194
 
MscI TGG'CCA

Cuts at:      0      85    1069    1631    2313 
   Size:         85     984     562     682

  Fragments arranged by size: 

                 984     682     562      85
 
MslI CAynn'nnrTG

Cuts at:      0       8      69     406    1693    1709    2131    2313 
   Size:          8      61     337    1287      16     422     182

  Fragments arranged by size: 

                1287     422     337     182      61      16       8
 
MspA1I CmG'CkG

Cuts at:      0     585     801     960     975    1354    1677    1683    1966 
   Size:        585     216     159      15     379     323       6     283

Cuts at:   1966    2313 
   Size:        347

  Fragments arranged by size: 

                 585     379     347     323     283     216     159      15
                   6
 
NaeI GCC'GGC

Cuts at:      0      57    2313 
   Size:         57    2256
 
NcoI C'CATG_G

Cuts at:      0     870    2313 
   Size:        870    1443
 
NgoMIV G'CCGG_C

Cuts at:      0      55    2313 
   Size:         55    2258
 
NspI r_CATG'y

Cuts at:      0    1452    2313 
   Size:       1452     861
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2221    2235    2313 
   Size:       2221      14      78

  Fragments arranged by size: 

                2221      78      14
 
PfoI T'CCnGG_A

Cuts at:      0     389    1223    2313 
   Size:        389     834    1090

  Fragments arranged by size: 

                1090     834     389
 
PpuMI rG'GwC_Cy

Cuts at:      0     888    1810    1951    2313 
   Size:        888     922     141     362

  Fragments arranged by size: 

                 922     888     362     141
 
PshAI GACnn'nnGTC

Cuts at:      0     886    2313 
   Size:        886    1427
 
PstI C_TGCA'G

Cuts at:      0     224     353     844    1682    2313 
   Size:        224     129     491     838     631

  Fragments arranged by size: 

                 838     631     491     224     129
 
PvuII CAG'CTG

Cuts at:      0     585     801     975    1677    1966    2313 
   Size:        585     216     174     702     289     347

  Fragments arranged by size: 

                 702     585     347     289     216     174
 
SacI G_AGCT'C

Cuts at:      0     713    2313 
   Size:        713    1600
 
SacII CC_GC'GG

Cuts at:      0     961    2313 
   Size:        961    1352
 
SanDI GG'GwC_CC

Cuts at:      0    1810    2313 
   Size:       1810     503
 
SapI GCTCTTCn'nnn_

Cuts at:      0     632    2313 
   Size:        632    1681
 
SexAI A'CCwGG_T

Cuts at:      0    1909    2313 
   Size:       1909     404
 
SfcI C'TryA_G

Cuts at:      0     220     349     840    1678    2313 
   Size:        220     129     491     838     635

  Fragments arranged by size: 

                 838     635     491     220     129
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2231    2313 
   Size:       2231      82
 
SmaI CCC'GGG

Cuts at:      0    1403    2313 
   Size:       1403     910
 
SmlI C'TyrA_G

Cuts at:      0     541    2313 
   Size:        541    1772
 
StuI AGG'CCT

Cuts at:      0     568    2234    2313 
   Size:        568    1666      79

  Fragments arranged by size: 

                1666     568      79
 
StyI C'CwwG_G

Cuts at:      0     179     870    1130    2229    2313 
   Size:        179     691     260    1099      84

  Fragments arranged by size: 

                1099     691     260     179      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     898    2313 
   Size:        898    1415
 
TatI w'GTAC_w

Cuts at:      0    1256    1451    1701    2313 
   Size:       1256     195     250     612

  Fragments arranged by size: 

                1256     612     250     195
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      72     104    1811    1843    2313 
   Size:         72      32    1707      32     470

  Fragments arranged by size: 

                1707     470      72      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2167    2313 
   Size:       2167     146
 
XmaI C'CCGG_G

Cuts at:      0    1401    2313 
   Size:       1401     912
 
XmnI GAAnn'nnTTC

Cuts at:      0     319     647    2313 
   Size:        319     328    1666

  Fragments arranged by size: 

                1666     328     319
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI
    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI     BtgI     BtsI     ClaI    CspCI
     DraI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI
   HincII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI    PflMI     PfoI    PpuMI    PshAI     PstI
    PvuII     SacI    SacII    SanDI     SapI    SexAI     SfcI     SfiI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI     AscI    AsiSI    AvrII     BaeI     BbeI
     BcgI     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV     FalI
     FseI    FspAI    HaeII    Hin4I  HindIII     KasI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SalI     SbfI     ScaI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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