Restriction map for pF1KSDA0513
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BspMI       BsrFI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp509I     Van91I      XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          255  GTCTAC               GT^[AC][GT]AC
AlwI          339  GGATC                GGATC(4/5)
AlwNI         583  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           46  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       428  AGCGCT               AGC^GCT
ApaI          760  GGGCCC               GGGCC^C
Asp700I       312  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         246  GAGCAC               G[AT]GC[AT]^C
AspI          937  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          784  GAAGAC               GAAGAC(2/6)
BglI          894  GCCCATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         784  GAAGAC               GAAGAC(2/6)
BsaWI        1055  TCCGGT               [AT]^CCGG[AT]
BsgI          475  GTGCAG               GTGCAG(16/14)
BsiHKAI       246  GAGCAC               G[AT]GC[AT]^C
BsmI          921  GAATGC               GAATGC(1/-1)
Bsp1407I      872  TGTACA               T^GTACA
BspHI         542  TCATGA               T^CATGA
BsrBI         682  GAGCGG               CCGCTC(-3/-3)
BsrDI        1139  GCAATG               GCAATG(2/0)
BsrGI         872  TGTACA               T^GTACA
BstAPI       1101  GCACAACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         46  CCTGAGG              CC^T[ACGT]AGG
DrdI          138  GACCACTGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      428  AGCGCT               AGC^GCT
Eco81I         46  CCTGAGG              CC^T[ACGT]AGG
EcoNI         701  CCTCGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         428  AGCGCT               [GA]GCGC^[CT]
HgaI          937  GACGC                GACGC(5/10)
KspI          614  CCGCGG               CCGC^GG
MslI          543  CATGACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI         1106  ACATGC               [GA]CATG^[CT]
PflFI         937  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          14  AGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          542  TCATGA               T^CATGA
SacII         614  CCGCGG               CCGC^GG
SapI         1183  GAAGAGC              GCTCTTC(1/4)
ScaI          368  AGTACT               AGT^ACT
SmaI          602  CCCGGG               CCC^GGG
SspBI         872  TGTACA               T^GTACA
Tth111I       937  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         602  CCCGGG               C^CCGGG
XmaI          602  CCCGGG               C^CCGGG
XmnI          312  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI          602  CCCGGG               C^[CT]CG[GA]G
              838  CCCGAG              
AvaII          15  GGTCC                G^G[AT]CC
              198  GGACC               
BalI          767  TGGCCA               TGG^CCA
             1223  TGGCCA              
BanII          24  GGGCTC               G[GA]GC[CT]^C
              760  GGGCCC              
BglII         314  AGATCT               A^GATCT
              863  AGATCT              
BsaAI         304  TACGTG               [CT]AC^GT[GA]
              415  TACGTG              
BsoBI         602  CCCGGG               C^[CT]CG[GA]G
              838  CCCGAG              
BstYI         314  AGATCT               [GA]^GATC[CT]
              863  AGATCT              
CfoI          429  GCGC                 GCG^C
             1041  GCGC                
DsaI           20  CCGTGG               C^C[GA][CT]GG
              614  CCGCGG              
FauI          613  CCCGC                CCCGC(4/6)
              616  GCGGG               
HhaI          429  GCGC                 GCG^C
             1041  GCGC                
HinP1I        429  GCGC                 G^CGC
             1041  GCGC                
HphI          557  TCACC                GGTGA(8/7)
             1073  TCACC               
MaeII         305  ACGT                 A^CGT
              416  ACGT                
MaeIII        823  GTGAC                ^GT[ACGT]AC
             1205  GTGAC               
MflI          314  AGATCT               [GA]^GATC[CT]
              863  AGATCT              
MluNI         767  TGGCCA               TGG^CCA
             1223  TGGCCA              
MscI          767  TGGCCA               TGG^CCA
             1223  TGGCCA              
PstI           91  CTGCAG               CTGCA^G
              372  CTGCAG              
SfcI           91  CTGCAG               C^T[GA][CT]AG
              372  CTGCAG              
Tsp45I        823  GTGAC                ^GT[GC]AC
             1205  GTGAC               
XhoII         314  AGATCT               [GA]^GATC[CT]
              863  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BanI        
BbvI        BcnI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TseI        TspRI       
TthHB8I     
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