Restriction map for pF1KSDA0482
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AgeI        
ApoI        AscI        AseI        AsnI        Asp700I     
AspI        AviII       AvrII       BbrPI       BclI        
BfrI        BlnI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco52I      EcoRI       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KspI        MfeI        MluI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tsp509I     Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI          694  GTCTAC               GT^[AC][GT]AC
AccIII       3563  TCCGGA               T^CCGGA
AhdI          349  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         349  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1537  GGCGCC               GGCGC^C
BglII        1643  AGATCT               A^GATCT
BsaAI        3370  TACGTG               [CT]AC^GT[GA]
BsaBI        1654  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3563  TCCGGA               [AT]^CCGG[AT]
BseAI        3563  TCCGGA               T^CCGGA
BspEI        3563  TCCGGA               T^CCGGA
BspLU11I      587  ACATGT               A^CATGT
DrdI         3412  GACCAGCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      349  GACAACCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1453  GATATC               GAT^ATC
HindIII       436  AAGCTT               A^AGCTT
KasI         1537  GGCGCC               G^GCGCC
MamI         1654  GATGTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3563  TCCGGA               T^CCGGA
MseI         3365  TTAA                 T^TAA
NarI         1537  GGCGCC               GG^CGCC
NspI          587  ACATGT               [GA]CATG^[CT]
SfoI         1537  GGCGCC               GGC^GCC
SmaI          389  CCCGGG               CCC^GGG
Tru9I        3365  TTAA                 T^TAA
XbaI          184  TCTAGA               T^CTAGA
XmaCI         389  CCCGGG               C^CCGGG
XmaI          389  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Alw44I       1333  GTGCAC               G^TGCAC
             3550  GTGCAC              
Aor51HI      2052  AGCGCT               AGC^GCT
             2519  AGCGCT              
ApaLI        1333  GTGCAC               G^TGCAC
             3550  GTGCAC              
BamHI         921  GGATCC               G^GATCC
             3381  GGATCC              
BbsI          793  GTCTTC               GAAGAC(2/6)
              889  GTCTTC              
BcgI         2494  CGACACCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3504  GCAGCAGCCTCG        
BciVI         651  GGATAC               GTATCC(6/5)
             1055  GGATAC              
BlpI         3052  GCTGAGC              GC^T[ACGT]AGC
             3343  GCTGAGC             
Bpu1102I     3052  GCTGAGC              GC^T[ACGT]AGC
             3343  GCTGAGC             
BpuAI         793  GTCTTC               GAAGAC(2/6)
              889  GTCTTC              
BsrFI          98  ACCGGC               [GA]^CCGG[CT]
             1481  ACCGGC              
BstEII       2742  GGTGACC              G^GT[ACGT]ACC
             3637  GGTCACC             
BstPI        2742  GGTGACC              G^GT[ACGT]ACC
             3637  GGTCACC             
BstXI        1563  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3627  CCAAGATCCTGG        
CelII        3052  GCTGAGC              GC^T[ACGT]AGC
             3343  GCTGAGC             
Cfr10I         98  ACCGGC               [GA]^CCGG[CT]
             1481  ACCGGC              
Eco47III     2052  AGCGCT               AGC^GCT
             2519  AGCGCT              
EcoO65I      2742  GGTGACC              G^GT[ACGT]ACC
             3637  GGTCACC             
PaeR7I       2672  CTCGAG               C^TCGAG
             2925  CTCGAG              
SapI         2368  GAAGAGC              GCTCTTC(1/4)
             2426  GCTCTTC             
SexAI        3356  ACCAGGT              A^CC[AT]GGT
             3434  ACCAGGT             
StuI           43  AGGCCT               AGG^CCT
              858  AGGCCT              
XcmI          593  CCACCTATACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2180  CCATCGTCCATGTGG     
XhoI         2672  CTCGAG               C^TCGAG
             2925  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        Asp718I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrBI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
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