Restriction map for pF1KSDA0478
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AscI        AseI        
AsnI        AspEI       AviII       AvrII       BalI        
BamHI       BbeI        BciVI       BfrI        BglI        
BlnI        BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BspEI       BssHII      Bst1107I    BstBI       
BstUI       BstXI       BstZ17I     CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MamI        MluI        MluNI       
MroI        MscI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Aor51HI      2267  AGCGCT               AGC^GCT
ApaI          504  GGGCCC               GGGCC^C
AspI         1044  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          202  CCCGAG               C^[CT]CG[GA]G
BbvCI        1255  GCTGAGG              CCTCAGC(-5/-2)
BcgI          204  CGAGGAGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2954  AGATCT               A^GATCT
BlpI         1887  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1887  GCTGAGC              GC^T[ACGT]AGC
BsmBI        1398  GAGACG               CGTCTC(1/5)
BsoBI         202  CCCGAG               C^[CT]CG[GA]G
BspDI         181  ATCGAT               AT^CGAT
BspLU11I     2422  ACATGT               A^CATGT
BsrBI        2980  GAGCGG               CCGCTC(-3/-3)
BsrDI        3353  CATTGC               GCAATG(2/0)
BsrFI          20  GCCGGC               [GA]^CCGG[CT]
BssSI        3652  CTCGTG               CACGAG(-5/-1)
CelII        1887  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         20  GCCGGC               [GA]^CCGG[CT]
ClaI          181  ATCGAT               AT^CGAT
DraI          783  TTTAAA               TTT^AAA
EaeI         1916  CGGCCA               [CT]^GGCC[GA]
Eco47III     2267  AGCGCT               AGC^GCT
EcoNI        2007  CCTTACTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1551  GATATC               GAT^ATC
HaeII        2267  AGCGCT               [GA]GCGC^[CT]
MfeI         3049  CAATTG               C^AATTG
MunI         3049  CAATTG               C^AATTG
NaeI           20  GCCGGC               GCC^GGC
NgoMIV         20  GCCGGC               G^CCGGC
PflFI        1044  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI         2687  AGTACT               AGT^ACT
SmlI          431  CTCAAG               C^T[CT][GA]AG
SspI          950  AATATT               AAT^ATT
Tth111I      1044  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I         91  GGTACC               G^GTACC
             3065  GGTACC              
AccI          296  GTCTAC               GT^[AC][GT]AC
             1003  GTAGAC              
AflIII       2422  ACATGT               A^C[GA][CT]GT
             2944  ACGTGT              
Alw44I       2257  GTGCAC               G^TGCAC
             2914  GTGCAC              
AocI         1734  CCTGAGG              CC^T[ACGT]AGG
             2456  CCTCAGG             
ApaLI        2257  GTGCAC               G^TGCAC
             2914  GTGCAC              
Asp718I        91  GGTACC               G^GTACC
             3065  GGTACC              
BbrPI        2731  CACGTG               CAC^GTG
             2943  CACGTG              
BclI         2127  TGATCA               T^GATCA
             3566  TGATCA              
BsaAI        2731  CACGTG               [CT]AC^GT[GA]
             2943  CACGTG              
BsaWI        2894  ACCGGA               [AT]^CCGG[AT]
             3311  TCCGGT              
Bsp1407I       41  TGTACA               T^GTACA
              233  TGTACA              
BspHI        1407  TCATGA               T^CATGA
             2609  TCATGA              
BsrGI          41  TGTACA               T^GTACA
              233  TGTACA              
BstEII        768  GGTTACC              G^GT[ACGT]ACC
             2991  GGTGACC             
BstPI         768  GGTTACC              G^GT[ACGT]ACC
             2991  GGTGACC             
Bsu36I       1734  CCTGAGG              CC^T[ACGT]AGG
             2456  CCTCAGG             
DraIII         45  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2986  CACATGGTG           
Eco81I       1734  CCTGAGG              CC^T[ACGT]AGG
             2456  CCTCAGG             
EcoO65I       768  GGTTACC              G^GT[ACGT]ACC
             2991  GGTGACC             
EcoRI         564  GAATTC               G^AATTC
             1141  GAATTC              
EcoT22I       185  ATGCAT               ATGCA^T
             2678  ATGCAT              
FauI         2726  CCCGC                CCCGC(4/6)
             3023  GCGGG               
FbaI         2127  TGATCA               T^GATCA
             3566  TGATCA              
KpnI           91  GGTACC               GGTAC^C
             3065  GGTACC              
NcoI         1898  CCATGG               C^CATGG
             2364  CCATGG              
NsiI          185  ATGCAT               ATGCA^T
             2678  ATGCAT              
PmaCI        2731  CACGTG               CAC^GTG
             2943  CACGTG              
PmlI         2731  CACGTG               CAC^GTG
             2943  CACGTG              
RcaI         1407  TCATGA               T^CATGA
             2609  TCATGA              
SapI         2199  GAAGAGC              GCTCTTC(1/4)
             3246  GCTCTTC             
SphI         2462  GCATGC               GCATG^C
             2680  GCATGC              
SspBI          41  TGTACA               T^GTACA
              233  TGTACA              
XbaI          732  TCTAGA               T^CTAGA
             1563  TCTAGA              
XcmI         2364  CCATGGTGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3045  CCAGCAATTGTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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