################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccI AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BbeI BbvCI BcgI BclI BlnI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HpaI KasI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 3278 GGTACC G^GTACC AccIII 762 TCCGGA T^CCGGA AflII 1045 CTTAAG C^TTAAG ApaI 3108 GGGCCC GGGCC^C Asp718I 3278 GGTACC G^GTACC AspI 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2347 CACGTG CAC^GTG BciVI 3560 GTATCC GTATCC(6/5) BfrI 1045 CTTAAG C^TTAAG BsaAI 2347 CACGTG [CT]AC^GT[GA] BsaWI 762 TCCGGA [AT]^CCGG[AT] BseAI 762 TCCGGA T^CCGGA BsiEI 930 CGACCG CG[GA][CT]^CG BsmBI 2704 CGTCTC CGTCTC(1/5) BspEI 762 TCCGGA T^CCGGA EcoT22I 903 ATGCAT ATGCA^T HaeII 1586 AGCGCC [GA]GCGC^[CT] HincII 814 GTCAAC GT[CT]^[GA]AC HindII 814 GTCAAC GT[CT]^[GA]AC HindIII 2182 AAGCTT A^AGCTT KpnI 3278 GGTACC GGTAC^C KspI 264 CCGCGG CCGC^GG MroI 762 TCCGGA T^CCGGA MseI 1046 TTAA T^TAA NsiI 903 ATGCAT ATGCA^T PflFI 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2347 CACGTG CAC^GTG PmlI 2347 CACGTG CAC^GTG SacI 490 GAGCTC GAGCT^C SacII 264 CCGCGG CCGC^GG SbfI 2856 CCTGCAGG CCTGCA^GG Sse8387I 2856 CCTGCAGG CCTGCA^GG Tru9I 1046 TTAA T^TAA Tth111I 1066 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3324 TCTAGA T^CTAGA XcmI 756 CCAGGATCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 1447 GGCGTC G[GA]^CG[CT]C 2617 GACGCC AocI 2448 CCTGAGG CC^T[ACGT]AGG 2976 CCTGAGG Asp700I 273 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 791 GAAAGCGTTC AvaI 335 CCCGGG C^[CT]CG[GA]G 1796 CCCGGG BalI 1052 TGGCCA TGG^CCA 1244 TGGCCA BamHI 752 GGATCC G^GATCC 759 GGATCC BbsI 2193 GAAGAC GAAGAC(2/6) 3385 GTCTTC BglI 2777 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3128 GCCCAGAGGGC BpuAI 2193 GAAGAC GAAGAC(2/6) 3385 GTCTTC BsaHI 1447 GGCGTC G[GA]^CG[CT]C 2617 GACGCC BsoBI 335 CCCGGG C^[CT]CG[GA]G 1796 CCCGGG BsrDI 125 GCAATG GCAATG(2/0) 1250 GCAATG BstXI 1997 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3428 CCATCCTTCTGG Bsu36I 2448 CCTGAGG CC^T[ACGT]AGG 2976 CCTGAGG DraIII 466 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 874 CACACGGTG DrdI 1801 GACGGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2866 GACACAGTGGTC EaeI 1052 TGGCCA [CT]^GGCC[GA] 1244 TGGCCA Eco81I 2448 CCTGAGG CC^T[ACGT]AGG 2976 CCTGAGG EcoNI 645 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1095 CCTCCGCCAGG Hin1I 1447 GGCGTC G[GA]^CG[CT]C 2617 GACGCC MluNI 1052 TGGCCA TGG^CCA 1244 TGGCCA MscI 1052 TGGCCA TGG^CCA 1244 TGGCCA NcoI 221 CCATGG C^CATGG 980 CCATGG NspI 2274 GCATGT [GA]CATG^[CT] 2786 GCATGT SmaI 335 CCCGGG CCC^GGG 1796 CCCGGG TaqI 1856 TCGA T^CGA 2053 TCGA TthHB8I 1856 TCGA T^CGA 2053 TCGA XmaCI 335 CCCGGG C^CCGGG 1796 CCCGGG XmaI 335 CCCGGG C^CCGGG 1796 CCCGGG XmnI 273 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 791 GAAAGCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I Tsp509I TspRI Van91I XhoII