Restriction map for pF1KSDA0448
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AvaI        AvaII       AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TfiI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII          60  CGCG                 CG^CG
AflIII        423  ACACGT               A^C[GA][CT]GT
AhdI          140  GACACGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI            9  CCTCAGG              CC^T[ACGT]AGG
AspEI         140  GACACGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         626  GTGCTC               G[AT]GC[AT]^C
BalI          666  TGGCCA               TGG^CCA
BamHI         492  GGATCC               G^GATCC
BclI          224  TGATCA               T^GATCA
BcnI          806  CCCGG                CC^[GC]GG
BsaI         1010  GAGACC               GGTCTC(1/5)
BsiHKAI       626  GTGCTC               G[AT]GC[AT]^C
BsmBI         564  GAGACG               CGTCTC(1/5)
BsmFI         108  GTCCC                GGGAC(10/14)
BsmI          679  GAATGC               GAATGC(1/-1)
BspMI         698  GCAGGT               ACCTGC(4/8)
BsrBI         111  CCGCTC               CCGCTC(-3/-3)
BstUI          60  CGCG                 CG^CG
Bsu36I          9  CCTCAGG              CC^T[ACGT]AGG
CfoI          366  GCGC                 GCG^C
Cfr13I        181  GGCCC                G^G[ACGT]CC
EaeI          666  TGGCCA               [CT]^GGCC[GA]
Eam1105I      140  GACACGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I          9  CCTCAGG              CC^T[ACGT]AGG
FauI          110  CCCGC                CCCGC(4/6)
FbaI          224  TGATCA               T^GATCA
HapII         807  CCGG                 C^CGG
HhaI          366  GCGC                 GCG^C
HinP1I        366  GCGC                 G^CGC
HinfI         106  GAGTC                G^A[ACGT]TC
HpaII         807  CCGG                 C^CGG
HphI          222  GGTGA                GGTGA(8/7)
MaeII         425  ACGT                 A^CGT
MluNI         666  TGGCCA               TGG^CCA
MscI          666  TGGCCA               TGG^CCA
MspA1I         37  CAGCTG               C[AC]G^C[GT]G
MspI          807  CCGG                 C^CGG
MvnI           60  CGCG                 CG^CG
NciI          806  CCCGG                CC^[GC]GG
PflMI          93  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          106  GAGTC                GAGTC(4/5)
PvuII          37  CAGCTG               CAG^CTG
SapI           72  GCTCTTC              GCTCTTC(1/4)
Sau96I        181  GGCCC                G^G[ACGT]CC
SfcI          910  CTACAG               C^T[GA][CT]AG
SspI          740  AATATT               AAT^ATT
TspRI         341  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         93  CCAGAAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          631  CCAGAGAAGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          448  AAATTT               [GA]^AATT[CT]
              952  GAATTT              
AlwNI          37  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
               94  CAGAAACTG           
ApoI          448  AAATTT               [GA]^AATT[CT]
              952  GAATTT              
AseI          478  ATTAAT               AT^TAAT
              733  ATTAAT              
AsnI          478  ATTAAT               AT^TAAT
              733  ATTAAT              
Asp700I       739  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              939  GAATGAGTTC          
BanII           4  GGGCTC               G[GA]GC[CT]^C
              983  GAGCCC              
BpmI          629  CTCCAG               CTGGAG(16/14)
              100  CTGGAG              
BseRI           7  CTCCTC               GAGGAG(10/8)
              103  GAGGAG              
BsiYI          93  CCAGAAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              677  CCGAATGCTGG         
BslI           93  CCAGAAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              677  CCGAATGCTGG         
BsrI           99  ACTGG                ACTGG(1/-1)
              340  CCAGT               
BstF5I       1020  CATCC                GGATG(2/0)
              177  GGATG               
BstYI          89  AGATCC               [GA]^GATC[CT]
              492  GGATCC              
Cac8I          42  GCTGGC               GC[ACGT]^[ACGT]GC
              253  GCAAGC              
EarI           73  CTCTTC               CTCTTC(1/4)
              211  GAAGAG              
FokI         1020  CATCC                GGATG(9/13)
              177  GGATG               
Ksp632I        73  CTCTTC               CTCTTC(1/4)
              211  GAAGAG              
MaeIII        558  GTTAC                ^GT[ACGT]AC
              757  GTTAC               
MflI           89  AGATCC               [GA]^GATC[CT]
              492  GGATCC              
MseI          479  TTAA                 T^TAA
              734  TTAA                
NlaIV           5  GGCTCC               GG[ACGT]^[ACGT]CC
              492  GGATCC              
PshBI         478  ATTAAT               AT^TAAT
              733  ATTAAT              
Tru9I         479  TTAA                 T^TAA
              734  TTAA                
XhoII          89  AGATCC               [GA]^GATC[CT]
              492  GGATCC              
XmnI          739  GAATATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              939  GAATGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BfaI        
BmyI        BsaJI       BsmAI       Bsp1286I    BsrDI       
BssKI       BstNI       DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      ItaI        MaeI        MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      Sau3AI      ScrFI       SfaNI       TaqI        
TseI        Tsp509I     TthHB8I     
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