Restriction map for pF1KSDA0420
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        AviII       AvrII       
BalI        BamHI       BbvCI       BciVI       BfrI        
BglI        BglII       BlnI        BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HindIII     HpaI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        520  GGTACC               G^GTACC
Alw44I        154  GTGCAC               G^TGCAC
AlwNI        1128  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         154  GTGCAC               G^TGCAC
Asp700I        72  GAAGCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       520  GGTACC               G^GTACC
AspI         1894  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BclI         1301  TGATCA               T^GATCA
BfaI         1147  CTAG                 C^TAG
BlpI         2025  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2025  GCTCAGC              GC^T[ACGT]AGC
BsiEI        1183  CGGCCG               CG[GA][CT]^CG
BspHI         819  TCATGA               T^CATGA
BsrFI         176  GCCGGC               [GA]^CCGG[CT]
BssSI        1567  CACGAG               CACGAG(-5/-1)
CelII        2025  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        176  GCCGGC               [GA]^CCGG[CT]
EagI         1183  CGGCCG               C^GGCCG
EarI         1486  GAAGAG               CTCTTC(1/4)
EclXI        1183  CGGCCG               C^GGCCG
Eco52I       1183  CGGCCG               C^GGCCG
FbaI         1301  TGATCA               T^GATCA
HincII       1069  GTCAAC               GT[CT]^[GA]AC
HindII       1069  GTCAAC               GT[CT]^[GA]AC
KpnI          520  GGTACC               GGTAC^C
Ksp632I      1486  GAAGAG               CTCTTC(1/4)
MaeI         1147  CTAG                 C^TAG
MslI          714  CACGCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          176  GCCGGC               GCC^GGC
NgoMIV        176  GCCGGC               G^CCGGC
PflFI        1894  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1566  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          819  TCATGA               T^CATGA
SacI          505  GAGCTC               GAGCT^C
SexAI         884  ACCAGGT              A^CC[AT]GGT
SmlI          864  CTTGAG               C^T[CT][GA]AG
StuI         1377  AGGCCT               AGG^CCT
Tsp45I       1810  GTGAC                ^GT[GC]AC
Tsp509I      1231  AATT                 ^AATT
Tth111I      1894  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1566  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           72  GAAGCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI           31  GTCTAC               GT^[AC][GT]AC
             1828  GTCTAC              
AhdI          397  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1242  GACCCTGGGTC         
AspEI         397  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1242  GACCCTGGGTC         
BbeI         1668  GGCGCC               GGCGC^C
             1681  GGCGCC              
BcgI          378  CGAGCAGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              795  GCACATCCTTCG        
BsrBI         641  CCGCTC               CCGCTC(-3/-3)
              166  GAGCGG              
Eam1105I      397  GACATTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1242  GACCCTGGGTC         
EcoNI         831  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              924  CCTGCTGGAGG         
EcoT14I       428  CCTTGG               C^C[AT][AT]GG
             1419  CCTTGG              
KasI         1668  GGCGCC               G^GCGCC
             1681  GGCGCC              
KspI          196  CCGCGG               CCGC^GG
             1554  CCGCGG              
MaeIII        756  GTTAC                ^GT[ACGT]AC
             1810  GTGAC               
NarI         1668  GGCGCC               GG^CGCC
             1681  GGCGCC              
NspI         1056  GCATGT               [GA]CATG^[CT]
             1166  ACATGC              
PstI          333  CTGCAG               CTGCA^G
             1875  CTGCAG              
SacII         196  CCGCGG               CCGC^GG
             1554  CCGCGG              
SfoI         1668  GGCGCC               GGC^GCC
             1681  GGCGCC              
StyI          428  CCTTGG               C^C[AT][AT]GG
             1419  CCTTGG              
TfiI          780  GATTC                G^A[AT]TC
             1999  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbrPI       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PmaCI       PmlI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        TaqI        TseI        TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI        
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