Restriction map for pF1KSDA0420
 (Linear) (Six-base) MAPSORT of: KIAA0420.seq  Check: 5320  from: 1  to: 2088



KIAA0420.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     208    2088 
   Size:        208    1880
 
AccI GT'mk_AC

Cuts at:      0      32    1829    2088 
   Size:         32    1797     259

  Fragments arranged by size: 

                1797     259      32
 
Acc65I G'GTAC_C

Cuts at:      0     520    2088 
   Size:        520    1568
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     886    1959    2088 
   Size:        886    1073     129

  Fragments arranged by size: 

                1073     886     129
 
AhdI GACnn_n'nnGTC

Cuts at:      0     402    1247    2088 
   Size:        402     845     841

  Fragments arranged by size: 

                 845     841     402
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     935    2088 
   Size:        935    1153
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1627    1659    2088 
   Size:       1627      32     429

  Fragments arranged by size: 

                1627     429      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1133    2088 
   Size:       1133     955
 
ApaI G_GGCC'C

Cuts at:      0     623     641    1928    2088 
   Size:        623      18    1287     160

  Fragments arranged by size: 

                1287     623     160      18
 
ApaLI G'TGCA_C

Cuts at:      0     154    2088 
   Size:        154    1934
 
AvaI C'yCGr_G

Cuts at:      0     842    1274    1685    1884    1921    2088 
   Size:        842     432     411     199      37     167

  Fragments arranged by size: 

                 842     432     411     199     167      37
 
BanI G'GyrC_C

Cuts at:      0     520     698    1172    1668    1681    2088 
   Size:        520     178     474     496      13     407

  Fragments arranged by size: 

                 520     496     474     407     178      13
 
BanII G_rGCy'C

Cuts at:      0     509     623     641     819    1272    1563    1804    1928 
   Size:        509     114      18     178     453     291     241     124

Cuts at:   1928    1984    2088 
   Size:         56     104

  Fragments arranged by size: 

                 509     453     291     241     178     124     114     104
                  56      18
 
BbeI G_GCGC'C

Cuts at:      0    1672    1685    2088 
   Size:       1672      13     403

  Fragments arranged by size: 

                1672     403      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      96     228     368    1272    2088 
   Size:         96     132     140     904     816

  Fragments arranged by size: 

                 904     816     140     132      96
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     367     401     784     818    2088 
   Size:        367      34     383      34    1270

  Fragments arranged by size: 

                1270     383     367      34      34
 
BclI T'GATC_A

Cuts at:      0    1301    2088 
   Size:       1301     787
 
BlpI GC'TnA_GC

Cuts at:      0    2026    2088 
   Size:       2026      62
 
Bme1580I G_kGCm'C

Cuts at:      0      91     158     623     641    1928    2088 
   Size:         91      67     465      18    1287     160

  Fragments arranged by size: 

                1287     465     160      91      67      18
 
BmgBI CAC'GTC

Cuts at:      0    1638    2088 
   Size:       1638     450
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      94     547    2088 
   Size:         94     453    1541

  Fragments arranged by size: 

                1541     453      94
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     528    2088 
   Size:        528    1560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     667     949    1174    1822    2067    2088 
   Size:        628      39     282     225     648     245      21

  Fragments arranged by size: 

                 648     628     282     245     225      39      21
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     885    2088 
   Size:        885    1203
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     292     757    1234    1522    2085    2088 
   Size:        292     465     477     288     563       3

  Fragments arranged by size: 

                 563     477     465     292     288       3
 
BsaAI yAC'GTr

Cuts at:      0      86     159     231    2088 
   Size:         86      73      72    1857

  Fragments arranged by size: 

                1857      86      73      72
 
BsaHI Gr'CG_yC

Cuts at:      0     191     544    1669    1682    1826    2088 
   Size:        191     353    1125      13     144     262

  Fragments arranged by size: 

                1125     353     262     191     144      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     586     616    1367    1397    1418    1445    1448    1475 
   Size:        586      30     751      30      21      27       3      27

Cuts at:   1475    1586    1616    2088 
   Size:        111      30     472

  Fragments arranged by size: 

                 751     586     472     111      30      30      30      27
                  27      21       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     268     577     886     946    1054    1504    2037    2040 
   Size:        268     309     309      60     108     450     533       3

Cuts at:   2040    2088 
   Size:         48

  Fragments arranged by size: 

                 533     450     309     309     268     108      60      48
                   3
 
BseYI C'CCAG_C

Cuts at:      0     598    1113    1677    1730    1861    2021    2088 
   Size:        598     515     564      53     131     160      67

  Fragments arranged by size: 

                 598     564     515     160     131      67      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1897    1989    2088 
   Size:        262    1635      92      99

  Fragments arranged by size: 

                1635     262      99      92
 
BsiEI CG_ry'CG

Cuts at:      0    1186    2088 
   Size:       1186     902
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     230     332     509     797    1292    1502    1553 
   Size:        158      72     102     177     288     495     210      51

Cuts at:   1553    1961    2088 
   Size:        408     127

  Fragments arranged by size: 

                 495     408     288     210     177     158     127     102
                  72      51
 
Bsp1286I G_dGCh'C

Cuts at:      0      91     158     230     332     509     623     641     797 
   Size:         91      67      72     102     177     114      18     156

Cuts at:    797     819    1272    1292    1502    1553    1563    1804    1928 
   Size:         22     453      20     210      51      10     241     124

Cuts at:   1928    1961    1984    2088 
   Size:         33      23     104

  Fragments arranged by size: 

                 453     241     210     177     156     124     114     104
                 102      91      72      67      51      33      23      22
                  20      18      10
 
BspHI T'CATG_A

Cuts at:      0     819    2088 
   Size:        819    1269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     208    1147    1841    2088 
   Size:        208     939     694     247

  Fragments arranged by size: 

                 939     694     247     208
 
BsrBI CCG'CTC

Cuts at:      0     168     643    2088 
   Size:        168     475    1445

  Fragments arranged by size: 

                1445     475     168
 
BsrFI r'CCGG_y

Cuts at:      0     176    2088 
   Size:        176    1912
 
BssSI C'ACGA_G

Cuts at:      0    1567    2088 
   Size:       1567     521
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     315     606    1869    2088 
   Size:        315     291    1263     219

  Fragments arranged by size: 

                1263     315     291     219
 
BstYI r'GATC_y

Cuts at:      0      95     891    1583    2088 
   Size:         95     796     692     505

  Fragments arranged by size: 

                 796     692     505      95
 
BtgI C'CryG_G

Cuts at:      0     196     615    1031    1554    1577    2088 
   Size:        196     419     416     523      23     511

  Fragments arranged by size: 

                 523     511     419     416     196      23
 
BtsI GCAGTG_nn'

Cuts at:      0     330    1525    2088 
   Size:        330    1195     563

  Fragments arranged by size: 

                1195     563     330
 
DraIII CAC_nnn'GTG

Cuts at:      0     162     888    1572    2088 
   Size:        162     726     684     516

  Fragments arranged by size: 

                 726     684     516     162
 
EaeI y'GGCC_r

Cuts at:      0     972    1183    1574    2055    2088 
   Size:        972     211     391     481      33

  Fragments arranged by size: 

                 972     481     391     211      33
 
EagI C'GGCC_G

Cuts at:      0    1183    2088 
   Size:       1183     905
 
EarI CTCTTCn'nnn_

Cuts at:      0    1481    2088 
   Size:       1481     607
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     982    1347    2088 
   Size:        982     365     741

  Fragments arranged by size: 

                 982     741     365
 
EcoICRI GAG'CTC

Cuts at:      0     507    2088 
   Size:        507    1581
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     667     886     949    1174    1822    1959    2067 
   Size:        628      39     219      63     225     648     137     108

Cuts at:   2067    2088 
   Size:         21

  Fragments arranged by size: 

                 648     628     225     219     137     108      63      39
                  21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     835     928    2088 
   Size:        835      93    1160

  Fragments arranged by size: 

                1160     835      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     594     620     637    1197    1369    1734    2088 
   Size:        594      26      17     560     172     365     354

  Fragments arranged by size: 

                 594     560     365     354     172      26      17
 
HaeII r_GCGC'y

Cuts at:      0    1048    1672    1685    2033    2088 
   Size:       1048     624      13     348      55

  Fragments arranged by size: 

                1048     624     348      55      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     420     586     618    1233    1265    1391    1423 
   Size:        388      32     166      32     615      32     126      32

Cuts at:   1423    1445    1477    1586    1618    2088 
   Size:         22      32     109      32     470

  Fragments arranged by size: 

                 615     470     388     166     126     109      32      32
                  32      32      32      32      22
 
HincII GTy'rAC

Cuts at:      0    1071    2088 
   Size:       1071    1017
 
KasI G'GCGC_C

Cuts at:      0    1668    1681    2088 
   Size:       1668      13     407

  Fragments arranged by size: 

                1668     407      13
 
KpnI G_GTAC'C

Cuts at:      0     524    2088 
   Size:        524    1564
 
MslI CAynn'nnrTG

Cuts at:      0     718    2088 
   Size:        718    1370
 
MspA1I CmG'CkG

Cuts at:      0     198    1113    1512    1556    1709    1716    2012    2088 
   Size:        198     915     399      44     153       7     296      76

  Fragments arranged by size: 

                 915     399     296     198     153      76      44       7
            
 
NaeI GCC'GGC

Cuts at:      0     178    2088 
   Size:        178    1910
 
NarI GG'CG_CC

Cuts at:      0    1669    1682    2088 
   Size:       1669      13     406

  Fragments arranged by size: 

                1669     406      13
 
NgoMIV G'CCGG_C

Cuts at:      0     176    2088 
   Size:        176    1912
 
NspI r_CATG'y

Cuts at:      0    1060    1170    2088 
   Size:       1060     110     918

  Fragments arranged by size: 

                1060     918     110
 
PasI CC'CwG_GG

Cuts at:      0     516     633    1245    2088 
   Size:        516     117     612     843

  Fragments arranged by size: 

                 843     612     516     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1572    2088 
   Size:       1572     516
 
PfoI T'CCnGG_A

Cuts at:      0    1132    1586    2088 
   Size:       1132     454     502

  Fragments arranged by size: 

                1132     502     454
 
PmlI CAC'GTG

Cuts at:      0      86     159     231    2088 
   Size:         86      73      72    1857

  Fragments arranged by size: 

                1857      86      73      72
 
PpuMI rG'GwC_Cy

Cuts at:      0     594    1369    1734    2088 
   Size:        594     775     365     354

  Fragments arranged by size: 

                 775     594     365     354
 
PspOMI G'GGCC_C

Cuts at:      0     619     637    1924    2088 
   Size:        619      18    1287     164

  Fragments arranged by size: 

                1287     619     164      18
 
PstI C_TGCA'G

Cuts at:      0     337    1879    2088 
   Size:        337    1542     209

  Fragments arranged by size: 

                1542     337     209
 
PvuII CAG'CTG

Cuts at:      0    1113    1512    1716    2088 
   Size:       1113     399     204     372

  Fragments arranged by size: 

                1113     399     372     204
 
SacI G_AGCT'C

Cuts at:      0     509    2088 
   Size:        509    1579
 
SacII CC_GC'GG

Cuts at:      0     199    1557    2088 
   Size:        199    1358     531

  Fragments arranged by size: 

                1358     531     199
 
SanDI GG'GwC_CC

Cuts at:      0     594    1369    2088 
   Size:        594     775     719

  Fragments arranged by size: 

                 775     719     594
 
SexAI A'CCwGG_T

Cuts at:      0     884    2088 
   Size:        884    1204
 
SfcI C'TryA_G

Cuts at:      0     333    1347    1875    2088 
   Size:        333    1014     528     213

  Fragments arranged by size: 

                1014     528     333     213
 
SfoI GGC'GCC

Cuts at:      0    1670    1683    2088 
   Size:       1670      13     405

  Fragments arranged by size: 

                1670     405      13
 
SmaI CCC'GGG

Cuts at:      0     844    1687    1886    1923    2088 
   Size:        844     843     199      37     165

  Fragments arranged by size: 

                 844     843     199     165      37
 
SmlI C'TyrA_G

Cuts at:      0     864    2088 
   Size:        864    1224
 
StuI AGG'CCT

Cuts at:      0    1379    2088 
   Size:       1379     709
 
StyI C'CwwG_G

Cuts at:      0     428    1419    2088 
   Size:        428     991     669

  Fragments arranged by size: 

                 991     669     428
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     392     603    1590    2088 
   Size:        392     211     987     498

  Fragments arranged by size: 

                 987     498     392     211
 
TatI w'GTAC_w

Cuts at:      0     452    2088 
   Size:        452    1636
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1367    1399    1418    1450    1498    1530    2088 
   Size:       1367      32      19      32      48      32     558

  Fragments arranged by size: 

                1367     558      48      32      32      32      19
 
Tth111I GACn'n_nGTC

Cuts at:      0    1897    2088 
   Size:       1897     191
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     315     967    1992    2088 
   Size:        315     652    1025      96

  Fragments arranged by size: 

                1025     652     315      96
 
XmaI C'CCGG_G

Cuts at:      0     842    1685    1884    1921    2088 
   Size:        842     843     199      37     167

  Fragments arranged by size: 

                 843     842     199     167      37
 
XmnI GAAnn'nnTTC

Cuts at:      0      76    2088 
   Size:         76    2012
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AlfI     AloI    AlwNI
     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BcgI
     BclI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BssSI    BstXI
    BstYI     BtgI     BtsI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI
     KpnI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII    SanDI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AleI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI
    BfrBI     BglI    BglII     BmtI   Bpu10I    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI     SapI     SbfI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XhoI     ZraI
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