Restriction map for pF1KSDA0398
 (Linear) (Six-base) MAPSORT of: KIAA0398.seq  Check: 1710  from: 1  to: 1428



KIAA0398.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AccI GT'mk_AC

Cuts at:      0     158    1169    1428 
   Size:        158    1011     259

  Fragments arranged by size: 

                1011     259     158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     451     974    1428 
   Size:        314     137     523     454

  Fragments arranged by size: 

                 523     454     314     137
 
AflII C'TTAA_G

Cuts at:      0    1365    1428 
   Size:       1365      63
 
AleI CACnn'nnGTG

Cuts at:      0     825    1428 
   Size:        825     603
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     448    1428 
   Size:        115     333     980

  Fragments arranged by size: 

                 980     333     115
 
ApoI r'AATT_y

Cuts at:      0       6      66     556     766     808    1030    1108    1189 
   Size:          6      60     490     210      42     222      78      81

Cuts at:   1189    1428 
   Size:        239

  Fragments arranged by size: 

                 490     239     222     210      81      78      60      42
                   6
 
AseI AT'TA_AT

Cuts at:      0      95    1428 
   Size:         95    1333
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      74    1428 
   Size:         74    1354
 
BglI GCCn_nnn'nGGC

Cuts at:      0     932    1428 
   Size:        932     496
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     131    1428 
   Size:        131    1297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1428 
   Size:        377    1051
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1428 
   Size:        412    1016
 
BsaHI Gr'CG_yC

Cuts at:      0     978    1428 
   Size:        978     450
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    1428 
   Size:        782     646
 
BsmI GAATG_Cn'

Cuts at:      0    1247    1428 
   Size:       1247     181
 
BsrDI GCAATG_nn'

Cuts at:      0    1126    1428 
   Size:       1126     302
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      10    1428 
   Size:         10    1418
 
BstEII G'GTnAC_C

Cuts at:      0    1349    1428 
   Size:       1349      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     235    1428 
   Size:        235    1193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     377     451     974    1428 
   Size:        314      63      74     523     454

  Fragments arranged by size: 

                 523     454     314      74      63
 
EcoRI G'AATT_C

Cuts at:      0      66    1108    1189    1428 
   Size:         66    1042      81     239

  Fragments arranged by size: 

                1042     239      81      66
 
EcoRV GAT'ATC

Cuts at:      0     594    1428 
   Size:        594     834
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     211    1370    1428 
   Size:        211    1159      58

  Fragments arranged by size: 

                1159     211      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249    1428 
   Size:        217      32    1179

  Fragments arranged by size: 

                1179     217      32
 
HindIII A'AGCT_T

Cuts at:      0     986    1428 
   Size:        986     442
 
MslI CAynn'nnrTG

Cuts at:      0     458     825    1428 
   Size:        458     367     603

  Fragments arranged by size: 

                 603     458     367
 
MspA1I CmG'CkG

Cuts at:      0     714    1428 
   Size:        714     714
 
NspI r_CATG'y

Cuts at:      0    1318    1428 
   Size:       1318     110
 
PasI CC'CwG_GG

Cuts at:      0     928    1428 
   Size:        928     500
 
PshAI GACnn'nnGTC

Cuts at:      0     142    1428 
   Size:        142    1286
 
PsiI TTA'TAA

Cuts at:      0     772    1428 
   Size:        772     656
 
PstI C_TGCA'G

Cuts at:      0     844    1428 
   Size:        844     584
 
SfcI C'TryA_G

Cuts at:      0     147     840    1428 
   Size:        147     693     588

  Fragments arranged by size: 

                 693     588     147
 
SmlI C'TyrA_G

Cuts at:      0     391    1365    1428 
   Size:        391     974      63

  Fragments arranged by size: 

                 974     391      63
 
SpeI A'CTAG_T

Cuts at:      0    1164    1428 
   Size:       1164     264
 
SspI AAT'ATT

Cuts at:      0      93    1428 
   Size:         93    1335
 
StuI AGG'CCT

Cuts at:      0    1249    1428 
   Size:       1249     179
 
StyI C'CwwG_G

Cuts at:      0    1250    1428 
   Size:       1250     178
 
TatI w'GTAC_w

Cuts at:      0     673    1151    1325    1428 
   Size:        673     478     174     103

  Fragments arranged by size: 

                 673     478     174     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     525     557    1428 
   Size:        525      32     871

  Fragments arranged by size: 

                 871     525      32
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AleI    AlwNI     ApoI     AseI     BbsI
     BglI     BmrI     BpmI    BpuEI    BsaHI     BsgI     BsmI    BsrDI
   BstAPI   BstEII     EarI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I
  HindIII     MslI   MspA1I     NspI     PasI    PshAI     PsiI     PstI
     SfcI     SmlI     SpeI     SspI     StuI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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