Restriction map for pF1KSDA0388
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BbeI        BbrPI       
BcgI        BciVI       BlnI        BlpI        Bpu1102I    
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
BspEI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MluI        MluNI       MroI        MscI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII        2233  GACGTC               GACGT^C
AccII        1963  CGCG                 CG^CG
AcyI         2233  GACGTC               G[GA]^CG[CT]C
AflII        1831  CTTAAG               C^TTAAG
AflIII       1985  ACATGT               A^C[GA][CT]GT
Alw44I        816  GTGCAC               G^TGCAC
ApaLI         816  GTGCAC               G^TGCAC
AvaI          267  CCCGGG               C^[CT]CG[GA]G
BamHI         499  GGATCC               G^GATCC
BbsI         1330  GTCTTC               GAAGAC(2/6)
BbvCI        1124  CCTCAGC              CCTCAGC(-5/-2)
BclI          449  TGATCA               T^GATCA
BfaI         1244  CTAG                 C^TAG
BfrI         1831  CTTAAG               C^TTAAG
BglI         2137  GCCAAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         621  AGATCT               A^GATCT
BpuAI        1330  GTCTTC               GAAGAC(2/6)
BsaAI        2209  TACGTG               [CT]AC^GT[GA]
BsaBI        2124  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2233  GACGTC               G[GA]^CG[CT]C
BsmBI         376  GAGACG               CGTCTC(1/5)
BsoBI         267  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1553  TGTACA               T^GTACA
BspDI         829  ATCGAT               AT^CGAT
BspLU11I     1985  ACATGT               A^CATGT
BsrBI        1059  CCGCTC               CCGCTC(-3/-3)
BsrFI         896  GCCGGC               [GA]^CCGG[CT]
BsrGI        1553  TGTACA               T^GTACA
BstUI        1963  CGCG                 CG^CG
CfoI          900  GCGC                 GCG^C
Cfr10I        896  GCCGGC               [GA]^CCGG[CT]
ClaI          829  ATCGAT               AT^CGAT
DraI          907  TTTAAA               TTT^AAA
DraII        1768  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI         1865  TGGCCG               [CT]^GGCC[GA]
EcoO109I     1768  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1909  GAATTC               G^AATTC
FbaI          449  TGATCA               T^GATCA
HaeII         899  GGCGCT               [GA]GCGC^[CT]
HgaI         1961  GACGC                GACGC(5/10)
HhaI          900  GCGC                 GCG^C
Hin1I        2233  GACGTC               G[GA]^CG[CT]C
HinP1I        900  GCGC                 G^CGC
HindIII       184  AAGCTT               A^AGCTT
MaeI         1244  CTAG                 C^TAG
MamI         2124  GATTGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          716  CAATTG               C^AATTG
MslI         1545  CACACAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          716  CAATTG               C^AATTG
MvnI         1963  CGCG                 CG^CG
NaeI          896  GCCGGC               GCC^GGC
NdeI          286  CATATG               CA^TATG
NgoMIV        896  GCCGGC               G^CCGGC
PvuII        1522  CAGCTG               CAG^CTG
ScaI          554  AGTACT               AGT^ACT
SmaI          267  CCCGGG               CCC^GGG
SspBI        1553  TGTACA               T^GTACA
SspI          391  AATATT               AAT^ATT
XcmI         2093  CCAAAGTGGTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         267  CCCGGG               C^CCGGG
XmaI          267  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI        1127  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1596  CAGCACCTG           
AvaII         341  GGTCC                G^G[AT]CC
             1382  GGACC               
BcnI          267  CCCGG                CC^[GC]GG
              268  CCGGG               
BpmI         1191  CTCCAG               CTGGAG(16/14)
              529  CTGGAG              
BsaI         2114  GAGACC               GGTCTC(1/5)
             2227  GAGACC              
BspHI        1457  TCATGA               T^CATGA
             1613  TCATGA              
BsrDI        1178  GCAATG               GCAATG(2/0)
             1710  GCAATG              
BstXI        1370  CCAGGCTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2118  CCATCGGATTGG        
DsaI          474  CCATGG               C^C[GA][CT]GG
             1335  CCATGG              
EcoT14I       474  CCATGG               C^C[AT][AT]GG
             1335  CCATGG              
FauI         1058  CCCGC                CCCGC(4/6)
              858  GCGGG               
MspA1I        856  CAGCGG               C[AC]G^C[GT]G
             1522  CAGCTG              
NciI          267  CCCGG                CC^[GC]GG
              268  CCGGG               
NcoI          474  CCATGG               C^CATGG
             1335  CCATGG              
PshAI        1750  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2229  GACCGACGTC          
PstI         1509  CTGCAG               CTGCA^G
             1812  CTGCAG              
RcaI         1457  TCATGA               T^CATGA
             1613  TCATGA              
SapI         2161  GAAGAGC              GCTCTTC(1/4)
             2166  GCTCTTC             
SfcI         1509  CTGCAG               C^T[GA][CT]AG
             1812  CTGCAG              
SmlI         1831  CTTAAG               C^T[CT][GA]AG
             2203  CTCAAG              
StyI          474  CCATGG               C^C[AT][AT]GG
             1335  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       BanI        BanII       
BbvI        BmyI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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