Restriction map for pF1KSDA0388
 (Linear) (Six-base) MAPSORT of: KIAA0388.seq  Check: 8578  from: 1  to: 2241



KIAA0388.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1609    1850    2241 
   Size:       1609     241     391

  Fragments arranged by size: 

                1609     391     241
 
AatII G_ACGT'C

Cuts at:      0    2237    2241 
   Size:       2237       4
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     693    1184    1331    1456    1546    2241 
   Size:         79     614     491     147     125      90     695

  Fragments arranged by size: 

                 695     614     491     147     125      90      79
 
AflII C'TTAA_G

Cuts at:      0    1831    2241 
   Size:       1831     410
 
AflIII A'CryG_T

Cuts at:      0    1985    2241 
   Size:       1985     256
 
AleI CACnn'nnGTG

Cuts at:      0    1549    2241 
   Size:       1549     692
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1132    1601    2241 
   Size:       1132     469     640

  Fragments arranged by size: 

                1132     640     469
 
ApaLI G'TGCA_C

Cuts at:      0     816    2241 
   Size:        816    1425
 
ApoI r'AATT_y

Cuts at:      0     132    1623    1909    2051    2241 
   Size:        132    1491     286     142     190

  Fragments arranged by size: 

                1491     286     190     142     132
 
AvaI C'yCGr_G

Cuts at:      0     267    2241 
   Size:        267    1974
 
BamHI G'GATC_C

Cuts at:      0     499    2241 
   Size:        499    1742
 
BanI G'GyrC_C

Cuts at:      0    1091    1339    1876    2241 
   Size:       1091     248     537     365

  Fragments arranged by size: 

                1091     537     365     248
 
BanII G_rGCy'C

Cuts at:      0    1457    1937    2168    2241 
   Size:       1457     480     231      73

  Fragments arranged by size: 

                1457     480     231      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1323    2241 
   Size:       1323     918
 
BbvCI CC'TCA_GC

Cuts at:      0    1125    2241 
   Size:       1125    1116
 
BclI T'GATC_A

Cuts at:      0     449    2241 
   Size:        449    1792
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2143    2241 
   Size:       2143      98
 
BglII A'GATC_T

Cuts at:      0     621    2241 
   Size:        621    1620
 
Bme1580I G_kGCm'C

Cuts at:      0     581     820    1879    2241 
   Size:        581     239    1059     362

  Fragments arranged by size: 

                1059     581     362     239
 
BmgBI CAC'GTC

Cuts at:      0    1391    2241 
   Size:       1391     850
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1194    1313    1689    1791    2241 
   Size:       1194     119     376     102     450

  Fragments arranged by size: 

                1194     450     376     119     102
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1227    1956    2241 
   Size:       1227     729     285

  Fragments arranged by size: 

                1227     729     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    1176    2241 
   Size:        550     626    1065

  Fragments arranged by size: 

                1065     626     550
 
Bpu10I CC'TnA_GC

Cuts at:      0    1125    2241 
   Size:       1125    1116
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2188    2241 
   Size:       2188      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2108    2221    2241 
   Size:       2108     113      20

  Fragments arranged by size: 

                2108     113      20
 
BsaAI yAC'GTr

Cuts at:      0    2211    2241 
   Size:       2211      30
 
BsaBI GATnn'nnATC

Cuts at:      0    2128    2241 
   Size:       2128     113
 
BsaHI Gr'CG_yC

Cuts at:      0    2234    2241 
   Size:       2234       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     398     428    1045    1075    1174    1204    2241 
   Size:        398      30     617      30      99      30    1037

  Fragments arranged by size: 

                1037     617     398      99      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     457     580     583    2241 
   Size:        457     123       3    1658

  Fragments arranged by size: 

                1658     457     123       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856     874    1487    1918    2241 
   Size:        856      18     613     431     323

  Fragments arranged by size: 

                 856     613     431     323      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     820    1072    1457    1781    1937    2168    2241 
   Size:        820     252     385     324     156     231      73

  Fragments arranged by size: 

                 820     385     324     252     231     156      73
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     370    2241 
   Size:        370    1871
 
Bsp1286I G_dGCh'C

Cuts at:      0     581     820    1072    1457    1781    1879    1937    2168 
   Size:        581     239     252     385     324      98      58     231

Cuts at:   2168    2241 
   Size:         73

  Fragments arranged by size: 

                 581     385     324     252     239     231      98      73
                  58
 
BspHI T'CATG_A

Cuts at:      0    1457    1613    2241 
   Size:       1457     156     628

  Fragments arranged by size: 

                1457     628     156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1389    1609    1850    2241 
   Size:       1389     220     241     391

  Fragments arranged by size: 

                1389     391     241     220
 
BsrBI CCG'CTC

Cuts at:      0    1061    2241 
   Size:       1061    1180
 
BsrDI GCAATG_nn'

Cuts at:      0    1185    1717    2241 
   Size:       1185     532     524

  Fragments arranged by size: 

                1185     532     524
 
BsrFI r'CCGG_y

Cuts at:      0     896    2241 
   Size:        896    1345
 
BsrGI T'GTAC_A

Cuts at:      0    1553    2241 
   Size:       1553     688
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     136     870    1007    1845    2241 
   Size:        136     734     137     838     396

  Fragments arranged by size: 

                 838     734     396     137     136
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1377    2125    2241 
   Size:       1377     748     116

  Fragments arranged by size: 

                1377     748     116
 
BstYI r'GATC_y

Cuts at:      0     161     499     621    2129    2241 
   Size:        161     338     122    1508     112

  Fragments arranged by size: 

                1508     338     161     122     112
 
BtgI C'CryG_G

Cuts at:      0     474    1335    2241 
   Size:        474     861     906

  Fragments arranged by size: 

                 906     861     474
 
BtsI GCAGTG_nn'

Cuts at:      0    1506    1736    2241 
   Size:       1506     230     505

  Fragments arranged by size: 

                1506     505     230
 
ClaI AT'CG_AT

Cuts at:      0     830    2241 
   Size:        830    1411
 
DraI TTT'AAA

Cuts at:      0     909    2241 
   Size:        909    1332
 
EaeI y'GGCC_r

Cuts at:      0    1865    2241 
   Size:       1865     376
 
EarI CTCTTCn'nnn_

Cuts at:      0     479    1545    2005    2156    2173    2241 
   Size:        479    1066     460     151      17      68

  Fragments arranged by size: 

                1066     479     460     151      68      17
 
EcoICRI GAG'CTC

Cuts at:      0    1455    1935    2166    2241 
   Size:       1455     480     231      75

  Fragments arranged by size: 

                1455     480     231      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     550     693    1176    1184    1331    1456    1546 
   Size:         79     471     143     483       8     147     125      90

Cuts at:   1546    2241 
   Size:        695

  Fragments arranged by size: 

                 695     483     471     147     143     125      90      79
                   8
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1769    2241 
   Size:       1769     472
 
EcoRI G'AATT_C

Cuts at:      0    1909    2241 
   Size:       1909     332
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1336    1419    1444    2241 
   Size:       1336      83      25     797

  Fragments arranged by size: 

                1336     797      83      25
 
HaeII r_GCGC'y

Cuts at:      0     903    2241 
   Size:        903    1338
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1174    1206    2241 
   Size:       1174      32    1035

  Fragments arranged by size: 

                1174    1035      32
 
HindIII A'AGCT_T

Cuts at:      0     184    2241 
   Size:        184    2057
 
MfeI C'AATT_G

Cuts at:      0     716    2241 
   Size:        716    1525
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     375    2241 
   Size:        219     156    1866

  Fragments arranged by size: 

                1866     219     156
 
MslI CAynn'nnrTG

Cuts at:      0    1549    2241 
   Size:       1549     692
 
MspA1I CmG'CkG

Cuts at:      0     858    1524    2241 
   Size:        858     666     717

  Fragments arranged by size: 

                 858     717     666
 
NaeI GCC'GGC

Cuts at:      0     898    2241 
   Size:        898    1343
 
NcoI C'CATG_G

Cuts at:      0     474    1335    2241 
   Size:        474     861     906

  Fragments arranged by size: 

                 906     861     474
 
NdeI CA'TA_TG

Cuts at:      0     287    2241 
   Size:        287    1954
 
NgoMIV G'CCGG_C

Cuts at:      0     896    2241 
   Size:        896    1345
 
NspI r_CATG'y

Cuts at:      0      72     655    1989    2241 
   Size:         72     583    1334     252

  Fragments arranged by size: 

                1334     583     252      72
 
PciI A'CATG_T

Cuts at:      0    1985    2241 
   Size:       1985     256
 
PshAI GACnn'nnGTC

Cuts at:      0    1754    2233    2241 
   Size:       1754     479       8

  Fragments arranged by size: 

                1754     479       8
 
PstI C_TGCA'G

Cuts at:      0    1513    1816    2241 
   Size:       1513     303     425

  Fragments arranged by size: 

                1513     425     303
 
PvuII CAG'CTG

Cuts at:      0    1524    2241 
   Size:       1524     717
 
SacI G_AGCT'C

Cuts at:      0    1457    1937    2168    2241 
   Size:       1457     480     231      73

  Fragments arranged by size: 

                1457     480     231      73
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2156    2173    2241 
   Size:       2156      17      68

  Fragments arranged by size: 

                2156      68      17
 
ScaI AGT'ACT

Cuts at:      0     556    2241 
   Size:        556    1685
 
SfcI C'TryA_G

Cuts at:      0    1509    1812    2241 
   Size:       1509     303     429

  Fragments arranged by size: 

                1509     429     303
 
SmaI CCC'GGG

Cuts at:      0     269    2241 
   Size:        269    1972
 
SmlI C'TyrA_G

Cuts at:      0    1831    2203    2241 
   Size:       1831     372      38

  Fragments arranged by size: 

                1831     372      38
 
SspI AAT'ATT

Cuts at:      0     393    2241 
   Size:        393    1848
 
StyI C'CwwG_G

Cuts at:      0     474    1335    2241 
   Size:        474     861     906

  Fragments arranged by size: 

                 906     861     474
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97     837    1592    2241 
   Size:         97     740     755     649

  Fragments arranged by size: 

                 755     740     649      97
 
TatI w'GTAC_w

Cuts at:      0     554    1553    2241 
   Size:        554     999     688

  Fragments arranged by size: 

                 999     688     554
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     398     430    2241 
   Size:        398      32    1811

  Fragments arranged by size: 

                1811     398      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2100    2241 
   Size:       2100     141
 
XmaI C'CCGG_G

Cuts at:      0     267    2241 
   Size:        267    1974
 
XmnI GAAnn'nnTTC

Cuts at:      0     688    1669    1909    2241 
   Size:        688     981     240     332

  Fragments arranged by size: 

                 981     688     332     240
 
ZraI GAC'GTC

Cuts at:      0    2235    2241 
   Size:       2235       6
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII   AflIII     AleI    AlwNI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BclI
     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI    BstXI    BstYI     BtgI     BtsI     ClaI     DraI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NspI     PciI    PshAI     PstI    PvuII     SacI
     SapI     ScaI     SfcI     SmaI     SmlI     SspI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI    BfrBI     BlpI     BmtI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BspEI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI     NarI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI
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