Restriction map for pF1KSDA0385
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Aor51HI     
AscI        AspI        AviII       AvrII       BbeI        
BcgI        BclI        BfrI        BlnI        BsiEI       
BsiWI       BspDI       BspHI       BsrBI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FbaI        FseI        FspI        HpaI        KasI        
KspI        MfeI        MluI        MunI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmeI        
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        369  GGTACC               G^GTACC
AccIII       3932  TCCGGA               T^CCGGA
AcyI         2527  GGCGTC               G[GA]^CG[CT]C
AhdI         1383  GACCGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        932  GTGCAC               G^TGCAC
ApaLI         932  GTGCAC               G^TGCAC
AseI         3019  ATTAAT               AT^TAAT
AsnI         3019  ATTAAT               AT^TAAT
Asp718I       369  GGTACC               G^GTACC
AspEI        1383  GACCGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1203  GGATCC               G^GATCC
BbrPI        1478  CACGTG               CAC^GTG
BciVI        2280  GGATAC               GTATCC(6/5)
BglI          840  GCCATTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1076  AGATCT               A^GATCT
BsaAI        1478  CACGTG               [CT]AC^GT[GA]
BsaBI        2673  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2527  GGCGTC               G[GA]^CG[CT]C
BseAI        3932  TCCGGA               T^CCGGA
BsmBI        2529  CGTCTC               CGTCTC(1/5)
Bsp1407I     1988  TGTACA               T^GTACA
BspEI        3932  TCCGGA               T^CCGGA
BspLU11I     1536  ACATGT               A^CATGT
BsrFI        1286  ACCGGC               [GA]^CCGG[CT]
BsrGI        1988  TGTACA               T^GTACA
Cfr10I       1286  ACCGGC               [GA]^CCGG[CT]
Eam1105I     1383  GACCGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        3901  GAATTC               G^AATTC
EcoT22I      2635  ATGCAT               ATGCA^T
Hin1I        2527  GGCGTC               G[GA]^CG[CT]C
HincII       1684  GTTGAC               GT[CT]^[GA]AC
HindII       1684  GTTGAC               GT[CT]^[GA]AC
HindIII      1959  AAGCTT               A^AGCTT
KpnI          369  GGTACC               GGTAC^C
MaeII        1479  ACGT                 A^CGT
MamI         2673  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3932  TCCGGA               T^CCGGA
MseI         3020  TTAA                 T^TAA
NsiI         2635  ATGCAT               ATGCA^T
PmaCI        1478  CACGTG               CAC^GTG
PmlI         1478  CACGTG               CAC^GTG
PshBI        3019  ATTAAT               AT^TAAT
SapI          996  GCTCTTC              GCTCTTC(1/4)
ScaI         3431  AGTACT               AGT^ACT
SmaI         3146  CCCGGG               CCC^GGG
SphI         4037  GCATGC               GCATG^C
SspBI        1988  TGTACA               T^GTACA
StuI         2840  AGGCCT               AGG^CCT
Tru9I        3020  TTAA                 T^TAA
XmaCI        3146  CCCGGG               C^CCGGG
XmaI         3146  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

ApaI          134  GGGCCC               GGGCC^C
             2293  GGGCCC              
Asp700I      1956  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3459  GAACATTTTC          
BalI         2936  TGGCCA               TGG^CCA
             2954  TGGCCA              
BlpI         3317  GCTCAGC              GC^T[ACGT]AGC
             3325  GCTGAGC             
Bpu1102I     3317  GCTCAGC              GC^T[ACGT]AGC
             3325  GCTGAGC             
BsaWI        3449  ACCGGA               [AT]^CCGG[AT]
             3932  TCCGGA              
BsmI          776  GCATTC               GAATGC(1/-1)
             1757  GCATTC              
BsrDI         937  CATTGC               GCAATG(2/0)
             1268  GCAATG              
BssSI        1258  CACGAG               CACGAG(-5/-1)
             1414  CACGAG              
CelII        3317  GCTCAGC              GC^T[ACGT]AGC
             3325  GCTGAGC             
DrdI          205  GACCCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1687  GACCGCACAGTC        
EaeI         2936  TGGCCA               [CT]^GGCC[GA]
             2954  TGGCCA              
HgaI         1213  GACGC                GACGC(5/10)
             2528  GCGTC               
MluNI        2936  TGGCCA               TGG^CCA
             2954  TGGCCA              
MscI         2936  TGGCCA               TGG^CCA
             2954  TGGCCA              
NdeI         1230  CATATG               CA^TATG
             3194  CATATG              
PshAI         286  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3802  GACACGGGTC          
SacI         1402  GAGCTC               GAGCT^C
             3328  GAGCTC              
XcmI          121  CCAACTCGAGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              227  CCACTGAGTTGCTGG     
XmnI         1956  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3459  GAACATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PaeR7I      
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoI        XhoII       
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